MysmA.00781.a

Beta-ketoadipyl CoA thiolase

CENTER ID: MysmA.00781.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00781.a.A1.GE28843 Full length( MysmA.00781.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00781.a.A1.PS00608 Full length( MysmA.00781.a ) 1 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5144
RefSeq: YP_889519.1
UniProt: A0R2Y1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAVIVATA RSPIGRAGKG SLVEMRPDDL AAQMVKAVLD KVPALDPRDI DDLIMGCGQP GGEAGFNIGR AVAVQLGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGH AFISAGVETV SRFAKGNADG WPDTKNPLYA DAMKRSDEAA AGTEEWHDPR EDGALPDVYI AMGQTAENVA LHTGISREDQ DHWGVRSQNR AEEAINSGFF EREITPVTLP DGTVVSKDDG PRAGTTYEKI SQLKPVFRPN GTVTAGNACP LNDGAAALVV MSDVRAKELG LTPLARIVST GVSGLSPEIM GLGPIEAVKK ALANANMTIG DIDLYEINEA FAVQVLGSAR ALGMDEDKLN VSGGAIALGH PFGMTGARIT ATLLNNLQTH DKTFGIETMC VGGGQGMAMV VERLS
NT Sequence
ATGCCTGAAG CCGTAATCGT TGCCACTGCC CGCTCCCCGA TCGGTCGCGC CGGTAAGGGG TCGCTCGTCG AGATGCGCCC CGACGACCTG GCCGCCCAGA TGGTCAAGGC CGTGCTCGAC AAGGTTCCCG CGCTCGATCC GCGCGACATC GACGACCTGA TCATGGGCTG CGGCCAGCCC GGCGGCGAGG CCGGGTTCAA CATCGGCCGT GCGGTCGCGG TGCAGCTCGG TTACGACTTC CTGCCCGGCA CCACGGTCAA CCGGTACTGC TCGTCGTCGC TGCAGACCAC CCGGATGGCA TTCCACGCCA TCAAGGCCGG TGAGGGGCAC GCGTTCATCT CCGCGGGCGT CGAGACCGTC TCGCGCTTCG CCAAGGGCAA CGCCGACGGC TGGCCCGACA CCAAGAACCC GCTGTACGCC GACGCCATGA AGCGTTCCGA TGAGGCGGCC GCGGGTACCG AGGAATGGCA CGACCCGCGT GAGGACGGCG CGCTGCCCGA CGTCTACATC GCCATGGGCC AGACCGCCGA GAACGTCGCG CTGCACACCG GCATCAGCCG TGAGGACCAG GACCACTGGG GCGTCCGCTC GCAGAACCGC GCCGAGGAGG CCATCAACAG CGGCTTCTTC GAGCGTGAGA TCACGCCGGT GACGCTGCCC GACGGCACCG TGGTGTCCAA GGACGACGGC CCGCGCGCCG GCACCACCTA CGAGAAGATC AGCCAGCTCA AGCCGGTGTT CCGGCCCAAC GGCACGGTCA CCGCGGGCAA CGCCTGCCCG CTCAACGACG GCGCCGCGGC GCTCGTGGTG ATGTCCGATG TGCGCGCCAA GGAACTCGGC CTGACACCGC TGGCCCGCAT CGTGTCGACG GGTGTTTCGG GGCTGTCGCC CGAGATCATG GGCCTCGGCC CGATCGAGGC CGTCAAGAAG GCCCTCGCCA ACGCGAACAT GACGATCGGC GACATCGACC TCTACGAGAT CAACGAGGCG TTCGCGGTGC AGGTCCTCGG CTCGGCACGC GCGCTCGGCA TGGACGAGGA CAAGCTCAAC GTCTCGGGCG GTGCGATCGC GCTCGGCCAT CCGTTCGGGA TGACCGGTGC CCGCATCACC GCGACGCTGC TCAACAATCT GCAGACCCAC GACAAGACCT TCGGCATCGA GACCATGTGT GTCGGTGGCG GTCAGGGCAT GGCAATGGTC GTGGAGCGCC TCTCC
Details for MysmA.00781.a.A1.GE28843
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVAT ARSPIGRAGK GSLVEMRPDD LAAQMVKAVL DKVPALDPRD IDDLIMGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFAKGNAD GWPDTKNPLY ADAMKRSDEA AAGTEEWHDP REDGALPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEEAINSGF FEREITPVTL PDGTVVSKDD GPRAGTTYEK ISQLKPVFRP NGTVTAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK KALANANMTI GDIDLYEINE AFAVQVLGSA RALGMDEDKL NVSXXAIALX HPFGMTGXRI TXTLXNNXQT XDKTFGIEX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctg aagccgtaat cgttgccact gcccgctccc cgatcggtcg cgccggtaag gggtcgctcg tcgagatgcg ccccgacgac ctggccgccc agatggtcaa ggccgtgctc gacaaggttc ccgcgctcga tccgcgcgac atcgacgacc tgatcatggg ctgcggccag cccggcggcg aggccgggtt caacatcggc cgtgcggtcg cggtgcagct cggttacgac ttcctgcccg gcaccacggt caaccggtac tgctcgtcgt cgctgcagac cacccggatg gcattccacg ccatcaaggc cggtgagggg cacgcgttca tctccgcggg cgtcgagacc gtctcgcgct tcgccaaggg caacgccgac ggctggcccg acaccaagaa cccgctgtac gccgacgcca tgaagcgttc cgatgaggcg gccgcgggta ccgaggaatg gcacgacccg cgtgaggacg gcgcgctgcc cgacgtctac atcgccatgg gccagaccgc cgagaacgtc gcgctgcaca ccggcatcag ccgtgaggac caggaccact ggggcgtccg ctcgcagaac cgcgccgagg aggccatcaa cagcggcttc ttcgagcgtg agatcacgcc ggtgacgctg cccgacggca ccgtggtgtc caaggacgac ggcccgcgcg ccggcaccac ctacgagaag atcagccagc tcaagccggt gttccggccc aacggcacgg tcaccgcggg caacgcctgc ccgctcaacg acggcgccgc ggcgctcgtg gtgatgtccg atgtgcgcgc caaggaactc ggcctgacac cgctggcccg catcgtgtcg acgggtgttt cggggctgtc gcccgagatc atgggcctcg gcccgatcga ggccgtcaag aaggccctcg ccaacgcgaa catgacgatc ggcgacatcg acctctacga gatcaacgag gcgttcgcgg tgcaggtcct cggctcggca cgcgcgctcg gcatggacga ggacaagctc aacgtctcnn nnnnngcgat cgcgctcgnc catccgttcg ggatgaccgg tncccgcatc accgngacgc tgntcaacaa tnngcagacc cangacaaga ccttcggcat cgagncca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVAT ARSPIGRAGK GSLVEMRPDD LAAQMVKAVL DKVPALDPRD IDDLIMGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFAKGNAD GWPDTKNPLY ADAMKRSDEA AAGTEEWHDP REDGALPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEEAINSGF FEREITPVTL PDGTVVSKDD GPRAGTTYEK ISQLKPVFRP NGTVTAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK KALANANMTI GDIDLYEINE AFAVQVLGSA RALGMDEDKL NVSGGAIALG HPFGMTGARI TATLLNNLQT HDKTFGIETM CVGGGQGMAM VVERLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgaagcc gtaatcgttg ccactgcccg ctccccgatc ggtcgcgccg gtaaggggtc gctcgtcgag atgcgccccg acgacctggc cgcccagatg gtcaaggccg tgctcgacaa ggttcccgcg ctcgatccgc gcgacatcga cgacctgatc atgggctgcg gccagcccgg cggcgaggcc gggttcaaca tcggccgtgc ggtcgcggtg cagctcggtt acgacttcct gcccggcacc acggtcaacc ggtactgctc gtcgtcgctg cagaccaccc ggatggcatt ccacgccatc aaggccggtg aggggcacgc gttcatctcc gcgggcgtcg agaccgtctc gcgcttcgcc aagggcaacg ccgacggctg gcccgacacc aagaacccgc tgtacgccga cgccatgaag cgttccgatg aggcggccgc gggtaccgag gaatggcacg acccgcgtga ggacggcgcg ctgcccgacg tctacatcgc catgggccag accgccgaga acgtcgcgct gcacaccggc atcagccgtg aggaccagga ccactggggc gtccgctcgc agaaccgcgc cgaggaggcc atcaacagcg gcttcttcga gcgtgagatc acgccggtga cgctgcccga cggcaccgtg gtgtccaagg acgacggccc gcgcgccggc accacctacg agaagatcag ccagctcaag ccggtgttcc ggcccaacgg cacggtcacc gcgggcaacg cctgcccgct caacgacggc gccgcggcgc tcgtggtgat gtccgatgtg cgcgccaagg aactcggcct gacaccgctg gcccgcatcg tgtcgacggg tgtttcgggg ctgtcgcccg agatcatggg cctcggcccg atcgaggccg tcaagaaggc cctcgccaac gcgaacatga cgatcggcga catcgacctc tacgagatca acgaggcgtt cgcggtgcag gtcctcggct cggcacgcgc gctcggcatg gacgaggaca agctcaacgt ctcgggcggt gcgatcgcgc tcggccatcc gttcgggatg accggtgccc gcatcaccgc gacgctgctc aacaatctgc agacccacga caagaccttc ggcatcgaga ccatgtgtgt cggtggcggt cagggcatgg caatggtcgt ggagcgcctc tccaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00781.a.A1.PS00608
PURIFICATION DATe: 5/18/2010
CONCENTRATION: 23.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVAT ARSPIGRAGK GSLVEMRPDD LAAQMVKAVL DKVPALDPRD IDDLIMGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFAKGNAD GWPDTKNPLY ADAMKRSDEA AAGTEEWHDP REDGALPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEEAINSGF FEREITPVTL PDGTVVSKDD GPRAGTTYEK ISQLKPVFRP NGTVTAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK KALANANMTI GDIDLYEINE AFAVQVLGSA RALGMDEDKL NVSXXAIALX HPFGMTGXRI TXTLXNNXQT XDKTFGIEX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctg aagccgtaat cgttgccact gcccgctccc cgatcggtcg cgccggtaag gggtcgctcg tcgagatgcg ccccgacgac ctggccgccc agatggtcaa ggccgtgctc gacaaggttc ccgcgctcga tccgcgcgac atcgacgacc tgatcatggg ctgcggccag cccggcggcg aggccgggtt caacatcggc cgtgcggtcg cggtgcagct cggttacgac ttcctgcccg gcaccacggt caaccggtac tgctcgtcgt cgctgcagac cacccggatg gcattccacg ccatcaaggc cggtgagggg cacgcgttca tctccgcggg cgtcgagacc gtctcgcgct tcgccaaggg caacgccgac ggctggcccg acaccaagaa cccgctgtac gccgacgcca tgaagcgttc cgatgaggcg gccgcgggta ccgaggaatg gcacgacccg cgtgaggacg gcgcgctgcc cgacgtctac atcgccatgg gccagaccgc cgagaacgtc gcgctgcaca ccggcatcag ccgtgaggac caggaccact ggggcgtccg ctcgcagaac cgcgccgagg aggccatcaa cagcggcttc ttcgagcgtg agatcacgcc ggtgacgctg cccgacggca ccgtggtgtc caaggacgac ggcccgcgcg ccggcaccac ctacgagaag atcagccagc tcaagccggt gttccggccc aacggcacgg tcaccgcggg caacgcctgc ccgctcaacg acggcgccgc ggcgctcgtg gtgatgtccg atgtgcgcgc caaggaactc ggcctgacac cgctggcccg catcgtgtcg acgggtgttt cggggctgtc gcccgagatc atgggcctcg gcccgatcga ggccgtcaag aaggccctcg ccaacgcgaa catgacgatc ggcgacatcg acctctacga gatcaacgag gcgttcgcgg tgcaggtcct cggctcggca cgcgcgctcg gcatggacga ggacaagctc aacgtctcnn nnnnngcgat cgcgctcgnc catccgttcg ggatgaccgg tncccgcatc accgngacgc tgntcaacaa tnngcagacc cangacaaga ccttcggcat cgagncca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVAT ARSPIGRAGK GSLVEMRPDD LAAQMVKAVL DKVPALDPRD IDDLIMGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFAKGNAD GWPDTKNPLY ADAMKRSDEA AAGTEEWHDP REDGALPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEEAINSGF FEREITPVTL PDGTVVSKDD GPRAGTTYEK ISQLKPVFRP NGTVTAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK KALANANMTI GDIDLYEINE AFAVQVLGSA RALGMDEDKL NVSGGAIALG HPFGMTGARI TATLLNNLQT HDKTFGIETM CVGGGQGMAM VVERLS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTGAAGCC GTAATCGTTG CCACTGCCCG CTCCCCGATC GGTCGCGCCG GTAAGGGGTC GCTCGTCGAG ATGCGCCCCG ACGACCTGGC CGCCCAGATG GTCAAGGCCG TGCTCGACAA GGTTCCCGCG CTCGATCCGC GCGACATCGA CGACCTGATC ATGGGCTGCG GCCAGCCCGG CGGCGAGGCC GGGTTCAACA TCGGCCGTGC GGTCGCGGTG CAGCTCGGTT ACGACTTCCT GCCCGGCACC ACGGTCAACC GGTACTGCTC GTCGTCGCTG CAGACCACCC GGATGGCATT CCACGCCATC AAGGCCGGTG AGGGGCACGC GTTCATCTCC GCGGGCGTCG AGACCGTCTC GCGCTTCGCC AAGGGCAACG CCGACGGCTG GCCCGACACC AAGAACCCGC TGTACGCCGA CGCCATGAAG CGTTCCGATG AGGCGGCCGC GGGTACCGAG GAATGGCACG ACCCGCGTGA GGACGGCGCG CTGCCCGACG TCTACATCGC CATGGGCCAG ACCGCCGAGA ACGTCGCGCT GCACACCGGC ATCAGCCGTG AGGACCAGGA CCACTGGGGC GTCCGCTCGC AGAACCGCGC CGAGGAGGCC ATCAACAGCG GCTTCTTCGA GCGTGAGATC ACGCCGGTGA CGCTGCCCGA CGGCACCGTG GTGTCCAAGG ACGACGGCCC GCGCGCCGGC ACCACCTACG AGAAGATCAG CCAGCTCAAG CCGGTGTTCC GGCCCAACGG CACGGTCACC GCGGGCAACG CCTGCCCGCT CAACGACGGC GCCGCGGCGC TCGTGGTGAT GTCCGATGTG CGCGCCAAGG AACTCGGCCT GACACCGCTG GCCCGCATCG TGTCGACGGG TGTTTCGGGG CTGTCGCCCG AGATCATGGG CCTCGGCCCG ATCGAGGCCG TCAAGAAGGC CCTCGCCAAC GCGAACATGA CGATCGGCGA CATCGACCTC TACGAGATCA ACGAGGCGTT CGCGGTGCAG GTCCTCGGCT CGGCACGCGC GCTCGGCATG GACGAGGACA AGCTCAACGT CTCGGGCGGT GCGATCGCGC TCGGCCATCC GTTCGGGATG ACCGGTGCCC GCATCACCGC GACGCTGCTC AACAATCTGC AGACCCACGA CAAGACCTTC GGCATCGAGA CCATGTGTGT CGGTGGCGGT CAGGGCATGG CAATGGTCGT GGAGCGCCTC TCCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA