MymaA.00781.a

Beta-ketoacyl CoA thiolase FadA3

CENTER ID: MymaA.00781.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00781.a.A1.GE34948 Full length( MymaA.00781.a ) 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4722
RefSeq: YP_001852655.1
UniProt: B2HT79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGAVMPEAV IVSTARSPIG RAMKGSLVSM RPDDLAVQMV RAALDKVPAL NPHQIDDLIM GCGLPGGESG FNIGRVVAVA LGYDFLPGTT VNRYCSSSLQ TTRMAFHAIK AGEGDAFISA GVETVSRFGK GNSDSLPDTK NPLFDEAQER TAAAAAGAEE WHDPRADGNL PDVYIAMGQT AENVAIISGI SREDQDHWGV RSQNRAEEAI KSGFFEREIS PVTLPDGTTV STDDGPRAGT TYEKVSQLKP VFRPNGTVTA GNACPLNDGA AAVVITSDTK AKELGLTPLA RIVSTGVSGL SPEIMGLGPI EACKKALQRA GMSISDIDLV EINEAFAVQV LGSARELGID EDKLNVSGGA IALGHPFGMT GARITTTLLN NLQTHDKTFG LETMCVGGGQ GMAMVIERLS
NT Sequence
ATGGAGGGAG CTGTTATGCC AGAAGCCGTC ATCGTCTCAA CAGCCCGCTC GCCGATCGGC CGCGCCATGA AAGGGTCGCT GGTCAGCATG CGCCCCGACG ACTTGGCCGT GCAGATGGTG CGCGCCGCGT TGGACAAAGT GCCCGCGCTG AACCCGCACC AGATCGACGA TCTGATCATG GGCTGCGGCC TCCCGGGAGG TGAGTCCGGC TTCAATATCG GGCGCGTCGT CGCCGTGGCG CTGGGCTACG ACTTTCTGCC GGGGACCACG GTCAACCGGT ACTGCTCGTC TTCACTGCAG ACCACCCGGA TGGCGTTTCA TGCGATCAAG GCCGGCGAGG GCGACGCGTT CATCTCCGCG GGCGTAGAGA CCGTGTCCCG GTTCGGCAAG GGCAACTCCG ACTCGCTGCC CGACACCAAG AACCCCCTCT TCGATGAGGC CCAGGAACGC ACCGCCGCCG CGGCCGCGGG TGCCGAGGAA TGGCATGACC CGCGCGCTGA CGGAAATCTG CCTGACGTCT ATATCGCCAT GGGCCAGACC GCTGAAAACG TCGCGATCAT TTCGGGCATC AGCCGCGAAG ACCAAGACCA CTGGGGTGTG CGCAGCCAAA ACCGTGCCGA AGAGGCGATC AAGAGCGGCT TTTTCGAACG GGAGATCTCC CCGGTGACGC TTCCCGACGG CACCACGGTC AGCACCGATG ATGGCCCGCG CGCGGGTACC ACCTACGAAA AGGTCAGCCA ACTCAAGCCG GTGTTTCGGC CCAACGGCAC AGTGACCGCC GGCAACGCCT GTCCACTCAA CGACGGCGCC GCGGCAGTGG TGATCACCAG CGACACCAAG GCCAAGGAGC TGGGCCTGAC GCCGTTGGCG CGCATCGTGT CCACCGGCGT GAGCGGTCTG TCCCCGGAGA TCATGGGCCT TGGCCCCATC GAGGCTTGCA AGAAGGCGCT ACAGCGGGCG GGTATGTCCA TCAGCGACAT CGACTTGGTC GAGATCAATG AAGCGTTCGC GGTGCAGGTG CTGGGCTCGG CCCGGGAGTT GGGCATCGAC GAGGACAAGC TGAACGTCTC TGGCGGAGCG ATCGCCCTGG GGCACCCGTT CGGCATGACC GGCGCCCGCA TCACCACCAC CTTGCTCAAC AATCTGCAGA CCCACGACAA GACGTTCGGC CTGGAGACCA TGTGCGTCGG TGGCGGCCAG GGCATGGCCA TGGTCATCGA GCGACTGAGC
Details for MymaA.00781.a.A1.GE34948
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 64
Validated AA Sequence
MGTLEAQTQG PGSMEGAVMP EAVIVSTARS PIGRAMKGSL VSMRPDDLAV QMVRAALDKV PALNPHQIDD LIMGCGLPGG ESGFNIGRVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGDAF ISAGVETVSR FGKGNSDSLP DTKNPLFDEA QERTAAAAAG AEEWHDPRAD GNLPDVYIAM GQTAENVAII SGISREDQDH WGVRSXNRAE EAIKSGFFER EISPVTLPDG TTVSTDDGPR AGTTYEKXSQ LXPVFRPNGT VTXGNA
Validated NT Sequence
ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggagggagc tgttatgcca gaagccgtca tcgtctcaac agcccgctcg ccgatcggcc gcgccatgaa agggtcgctg gtcagcatgc gccccgacga cttggccgtg cagatggtgc gcgccgcgtt ggacaaagtg cccgcgctga acccgcacca gatcgacgat ctgatcatgg gctgcggcct cccgggaggt gagtccggct tcaatatcgg gcgcgtcgtc gccgtggcgc tgggctacga ctttctgccg gggaccacgg tcaaccggta ctgctcgtct tcactgcaga ccacccggat ggcgtttcat gcgatcaagg ccggcgaggg cgacgcgttc atctccgcgg gcgtagagac cgtgtcccgg ttcggcaagg gcaactccga ctcgctgccc gacaccaaga accccctctt cgatgaggcc caggaacgca ccgccgccgc ggccgcgggt gccgaggaat ggcatgaccc gcgcgctgac ggaaatctgc ctgacgtcta tatcgccatg ggccagaccg ctgaaaacgt cgcgatcatt tcgggcatca gccgcgaaga ccaagaccac tggggtgtgc gcagccnaaa ccgtgccgaa gaggcgatca agagcggctt tttcgaacgg gagatctccc cggtgacgct tcccgacggc accacggtca gcaccgatga tggcccgcgc gcgggtacca cctacgaaaa ggncagccaa ctcnagccgg tgtttcggcc caacggcaca gtgaccnccg gnaacgcctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGAVMPEA VIVSTARSPI GRAMKGSLVS MRPDDLAVQM VRAALDKVPA LNPHQIDDLI MGCGLPGGES GFNIGRVVAV ALGYDFLPGT TVNRYCSSSL QTTRMAFHAI KAGEGDAFIS AGVETVSRFG KGNSDSLPDT KNPLFDEAQE RTAAAAAGAE EWHDPRADGN LPDVYIAMGQ TAENVAIISG ISREDQDHWG VRSQNRAEEA IKSGFFEREI SPVTLPDGTT VSTDDGPRAG TTYEKVSQLK PVFRPNGTVT AGNACPLNDG AAAVVITSDT KAKELGLTPL ARIVSTGVSG LSPEIMGLGP IEACKKALQR AGMSISDIDL VEINEAFAVQ VLGSARELGI DEDKLNVSGG AIALGHPFGM TGARITTTLL NNLQTHDKTF GLETMCVGGG QGMAMVIERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagggagct gttatgccag aagccgtcat cgtctcaaca gcccgctcgc cgatcggccg cgccatgaaa gggtcgctgg tcagcatgcg ccccgacgac ttggccgtgc agatggtgcg cgccgcgttg gacaaagtgc ccgcgctgaa cccgcaccag atcgacgatc tgatcatggg ctgcggcctc ccgggaggtg agtccggctt caatatcggg cgcgtcgtcg ccgtggcgct gggctacgac tttctgccgg ggaccacggt caaccggtac tgctcgtctt cactgcagac cacccggatg gcgtttcatg cgatcaaggc cggcgagggc gacgcgttca tctccgcggg cgtagagacc gtgtcccggt tcggcaaggg caactccgac tcgctgcccg acaccaagaa ccccctcttc gatgaggccc aggaacgcac cgccgccgcg gccgcgggtg ccgaggaatg gcatgacccg cgcgctgacg gaaatctgcc tgacgtctat atcgccatgg gccagaccgc tgaaaacgtc gcgatcattt cgggcatcag ccgcgaagac caagaccact ggggtgtgcg cagccaaaac cgtgccgaag aggcgatcaa gagcggcttt ttcgaacggg agatctcccc ggtgacgctt cccgacggca ccacggtcag caccgatgat ggcccgcgcg cgggtaccac ctacgaaaag gtcagccaac tcaagccggt gtttcggccc aacggcacag tgaccgccgg caacgcctgt ccactcaacg acggcgccgc ggcagtggtg atcaccagcg acaccaaggc caaggagctg ggcctgacgc cgttggcgcg catcgtgtcc accggcgtga gcggtctgtc cccggagatc atgggccttg gccccatcga ggcttgcaag aaggcgctac agcgggcggg tatgtccatc agcgacatcg acttggtcga gatcaatgaa gcgttcgcgg tgcaggtgct gggctcggcc cgggagttgg gcatcgacga ggacaagctg aacgtctctg gcggagcgat cgccctgggg cacccgttcg gcatgaccgg cgcccgcatc accaccacct tgctcaacaa tctgcagacc cacgacaaga cgttcggcct ggagaccatg tgcgtcggtg gcggccaggg catggccatg gtcatcgagc gactgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa