MythA.00781.a

Beta-ketoadipyl CoA thiolase (A0R2Y1 homolog)

CENTER ID: MythA.00781.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00781.a.A1.GE28759 Full length( MythA.00781.a ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.583
RefSeq: WP_003924038.1
UniProt: G7CC97

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRANKGSLV DMRPDDLAAQ MVRAALDKVP QLDPRDIDDL IMGCGQPAGE AGNNIARVVA VQLGYDFLPG TTVNRYCSSS LQTSRMAFHA IKAGEGHAFI SAGVETVSRF GKGTADGWPD TKNPKFAEAM KRTEQAAAGG EEWHDPREDG NLPDIYIAMG QTAENVALYT GISREDQDHW GVRSQNRAEE AIKNGFFERE ITPVTLPDGT VVSTDDGPRP GTTYEKVSQL KPVFRPNGTV TAGNACPLND GAAALVIMSD VKARELGLTP LARIVSTGVS GLSPEIMGLG PIEATKKALA NAGMTINDVD LYEINEAFAV QVLGSARELG MDLDRLNVSG GAIALGHPFG MTGARITATL INNLQTHDKQ FGLETMCVGG GQGMAMIIER LS
NT Sequence
ATCGGCCGCG CCAACAAGGG TTCGCTGGTC GACATGCGAC CCGACGATCT GGCCGCCCAG ATGGTGCGGG CGGCGTTGGA CAAGGTGCCG CAGCTCGATC CGCGTGACAT CGACGACCTG ATCATGGGCT GCGGTCAGCC CGCGGGCGAG GCCGGCAACA ACATCGCCCG AGTGGTGGCC GTGCAGCTGG GTTACGACTT CCTGCCCGGC ACCACGGTCA ACCGGTACTG CTCGTCGTCG CTGCAGACCA GCCGGATGGC GTTCCACGCG ATCAAGGCGG GCGAGGGCCA CGCCTTCATC TCGGCGGGAG TGGAGACCGT GTCGCGGTTC GGCAAGGGCA CCGCCGACGG CTGGCCGGAC ACCAAGAACC CGAAGTTCGC CGAGGCGATG AAGCGCACCG AGCAGGCCGC GGCGGGCGGC GAGGAGTGGC ACGATCCTCG CGAGGACGGC AACCTGCCCG ACATCTACAT CGCGATGGGG CAGACCGCGG AGAACGTCGC GCTGTACACC GGCATCAGCC GCGAGGACCA GGACCACTGG GGTGTGCGGT CGCAGAACCG GGCCGAGGAG GCCATCAAGA ACGGCTTCTT CGAACGGGAG ATCACCCCGG TCACGTTGCC GGACGGCACG GTGGTCAGCA CCGATGACGG ACCGCGGCCC GGCACCACCT ACGAGAAGGT CAGCCAGCTC AAGCCGGTGT TCCGGCCCAA CGGCACCGTC ACCGCCGGCA ACGCGTGCCC GCTCAACGAC GGGGCCGCCG CGCTGGTGAT CATGTCCGAC GTGAAGGCCC GCGAGTTGGG GTTGACGCCG CTGGCGCGAA TCGTCTCGAC CGGGGTGAGC GGCCTGTCGC CCGAGATCAT GGGTCTCGGC CCGATCGAAG CGACCAAGAA GGCGCTCGCC AACGCCGGCA TGACGATCAA CGACGTCGAC CTCTACGAGA TCAACGAGGC GTTCGCGGTA CAGGTGCTGG GCTCGGCGCG CGAACTCGGC ATGGATCTGG ACCGGCTGAA CGTCTCGGGC GGCGCCATCG CCCTCGGTCA CCCGTTCGGG ATGACCGGTG CCCGCATCAC CGCGACGCTG ATCAACAACC TGCAGACCCA CGACAAGCAG TTCGGGTTGG AGACCATGTG CGTCGGTGGT GGCCAGGGCA TGGCGATGAT CATCGAGCGG TTGTCC
Details for MythA.00781.a.A1.GE28759
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGRANKGS LVDMRPDDLA AQMVRAALDK VPQLDPRDID DLIMGCGQPA GEAGNNIARV VAVQLGYDFL PGTTVNRYCS SSLQTSRMAF HAIKAGEGHA FISAGVETVS RFGKGTADGW PDTKNPKFAE AMKRTEQAAA GGEEWHDPRE DGNLPDIYIA MGQTAENVAL YTGISREDQD HWGVRSQNRA EEAIKNGFFE REITPVTLPD GTVVSTDDGP RPGTTYEKVS QLKPVFRPNG TVTAGNACPL NDGAAALVIM SDVKARELGL TPLARIVSTG VSGLSPEIMG LGPIEATKKA LANAGMTIND VDLYEINEAF AVQVLGSARE LGMDLDRLNV SGGAIALGHP FGMTGARITA TLINNLQTHD KQFGLETMCV GGGQGMAMII ERLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcggccgc gccaacaagg gttcgctggt cgacatgcga cccgacgatc tggccgccca gatggtgcgg gcggcgttgg acaaggtgcc gcagctcgat ccgcgtgaca tcgacgacct gatcatgggc tgcggtcagc ccgcgggcga ggccggcaac aacatcgccc gagtggtggc cgtgcagctg ggttacgact tcctgcccgg caccacggtc aaccggtact gctcgtcgtc gctgcagacc agccggatgg cgttccacgc gatcaaggcg ggcgagggcc acgccttcat ctcggcggga gtggagaccg tgtcgcggtt cggcaagggc accgccgacg gctggccgga caccaagaac ccgaagttcg ccgaggcgat gaagcgcacc gagcaggccg cggcgggcgg cgaggagtgg cacgatcctc gcgaggacgg caacctgccc gacatctaca tcgcgatggg gcagaccgcg gagaacgtcg cgctgtacac cggcatcagc cgcgaggacc aggaccactg gggtgtgcgg tcgcagaacc gggccgagga ggccatcaag aacggcttct tcgaacggga gatcaccccg gtcacgttgc cggacggcac ggtggtcagc accgatgacg gaccgcggcc cggcaccacc tacgagaagg tcagccagct caagccggtg ttccggccca acggcaccgt caccgccggc aacgcgtgcc cgctcaacga cggggccgcc gcgctggtga tcatgtccga cgtgaaggcc cgcgagttgg ggttgacgcc gctggcgcga atcgtctcga ccggggtgag cggcctgtcg cccgagatca tgggtctcgg cccgatcgaa gcgaccaaga aggcgctcgc caacgccggc atgacgatca acgacgtcga cctctacgag atcaacgagg cgttcgcggt acaggtgctg ggctcggcgc gcgaactcgg catggatctg gaccggctga acgtctcggg cggcgccatc gccctcggtc acccgttcgg gatgaccggt gcccgcatca ccgcgacgct gatcaacaac ctgcagaccc acgacaagca gttcgggttg gagaccatgt gcgtcggtgg tggccagggc atggcgatga tcatcgagcg gttgtccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa