MythA.00781.c

Beta-ketoadipyl CoA thiolase (A0R2Y1 homolog)

CENTER ID: MythA.00781.c
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00781.c.A1.GE28761 Full length( MythA.00781.c ) 1 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3188
RefSeq: WP_003926720.1
UniProt: G7CHZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQRRRGVTN QGATGLLRGR AFGSPPHTEA LWTSEHVPHD QVGDGGRLHG SVDFDGVPHD QVHRVIPHLD GIDDFRAHSD SATRDHRSQK ADLVQPVVEA HSGVFDAVVV LAQPGDQREC QKAVCDRRAE RALAFRSLDI HVDPLVIDGE LGELVDACLV HLEPPTDTEL LAYRLERLID GTDRFHANSC QPPNLYLFNG ILCKSTKGAR LGLSDDPGRN MTSRXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXGAV DFDDLVLAES LQGGGDSARY IAVNLGLTAI PGIAVNRQCA SSLSAIAMAA GQVAAGMSRA VLAGGMESCS TTPTMRKRKP FTSGTAPEDY EDPWLPFSHP PTDDAPALDM SITVGHNCAV QYGVTREDQD EWALRSHQRA VRAIDAGLFV DEIVPVKVPQ ADGSTTVFAQ DEHPRRDTNR DVLAGLKVLH PEIEGFTVTA GNSSGINDAA AVVALARPGT NHPVLANVLS WATVGVEPKC TGSGPISAIL KTLDLARRKI KDVALFEINE AFAAQSVACA RELGLDEEIV NVYGSGISLG HPIAATGARM VTSAIYELRR RGGGLGVLSM CAGGGMGAAM LIEVA
NT Sequence
ATCGCCCAGC GCCGTCGAGG CGTCACCAAT CAGGGTGCCA CCGGGCTTCT CCGCGGCCGG GCATTCGGCA GTCCGCCCCA CACCGAAGCG TTGTGGACAT CAGAACACGT ACCGCACGAT CAAGTCGGCG ACGGCGGCCG GCTTCACGGA TCCGTCGATT TCGACGGTGT GCCGCACGAT CAAGTTCACC GTGTCATCCC CCACCTTGAC GGCATCGACG ACTTCCGAGC GCACTCGGAC TCGGCTACCC GCGATCACCG GAGCCAGAAA GCGGACCTTG TTCAGCCCGT AGTTGAGGCC CATTCTGGCG TTTTCGACGC GGTAGTTGTC CTTGCTCAAC CCGGGGATCA GCGAGAGTGT CAGAAAGCCG TGTGCGATCG TCGCGCCGAA AGGGCTTTGG CTTTTCGCTC GCTCGACATC CACGTGGATC CACTGGTGAT CGACGGTGAG CTCGGCGAAC TGGTCGATGC GTGCCTGGTC CACCTCGAAC CACCGACTGA CACCGAGCTC CTCGCCTATC GCCTTGAGCG CCTCATTGAC GGAACCGATC GTTTTCATGC AAACTCCTGC CAACCGCCAA ATCTATATCT ATTTAACGGT ATACTCTGCA AAAGTACCAA AGGCGCCCGT TTGGGCCTGT CCGACGACCC CGGGAGAAAT ATGACATCGC GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGGGCGGTC GACTTCGACG ACCTTGTGCT TGCGGAAAGC CTGCAGGGCG GCGGGGACAG CGCCCGCTAT ATCGCTGTCA ACCTGGGCCT GACGGCGATT CCAGGGATAG CCGTCAACCG GCAGTGCGCC TCGAGCCTGT CCGCGATCGC GATGGCTGCA GGGCAGGTGG CCGCCGGAAT GAGCCGTGCC GTGCTTGCCG GGGGCATGGA GTCGTGTTCA ACCACACCGA CAATGCGCAA GCGCAAGCCC TTTACCTCGG GTACAGCGCC GGAAGACTAT GAGGACCCGT GGTTGCCGTT CTCACATCCA CCGACCGACG ACGCGCCCGC CCTGGACATG TCGATCACGG TGGGGCACAA CTGCGCAGTC CAGTACGGCG TTACCCGCGA GGACCAAGAC GAATGGGCGC TGCGCAGCCA TCAGCGCGCG GTGAGGGCAA TCGATGCCGG CCTATTCGTC GACGAGATCG TGCCAGTCAA GGTGCCGCAG GCCGACGGAT CGACGACCGT CTTTGCACAG GACGAGCATC CGCGCCGCGA CACCAACCGA GATGTGCTAG CCGGGCTAAA GGTGCTTCAC CCGGAAATCG AGGGCTTCAC GGTGACGGCC GGGAACTCGT CGGGCATCAA CGATGCTGCA GCTGTGGTCG CACTGGCCAG GCCCGGCACC AATCATCCCG TCCTGGCTAA CGTGCTGTCG TGGGCGACGG TCGGCGTCGA ACCGAAATGC ACCGGCAGCG GCCCGATTTC GGCCATTCTC AAGACGCTCG ACCTCGCCCG TCGCAAGATC AAGGACGTCG CGCTCTTTGA GATCAACGAG GCGTTCGCTG CCCAGTCGGT GGCCTGCGCC CGAGAACTCG GTCTCGATGA GGAGATCGTG AACGTGTACG GATCCGGTAT CAGCCTGGGC CACCCGATCG CGGCCACTGG AGCGCGGATG GTGACGTCGG CAATCTACGA GTTGCGCCGC CGCGGTGGTG GACTCGGAGT CCTGTCGATG TGCGCAGGCG GCGGAATGGG AGCGGCAATG TTAATCGAGG TGGCA
Details for MythA.00781.c.A1.GE28761
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIAQRRRGV TNQGATGLLR GRAFGSPPHT EALWTSEHVP HDQVGDGGRL HGSVDFDGVP HDQVHRVIPH LDGIDDFRAH SDSATRDHRS QKADLVQPVV EAHSGVFDAV VVLAQPGDQR ECQKAVCDRR AERALAFRSL DIHVDPLVID GELGELVDAC LVHLEPPTDT ELLAYRLERL IDGTDRFHAN SCQPPNLYLF NGILCKSTKG ARLGLSDDPG RNMTSXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXG AVDFDDLVLA ESLQGGGDSA RYIAVNLGLT AIPGIAVNRQ CASSLSAIAM AAGQVAAGMS RAVLAGGMES CSTTPTMRKR KPFTSGTAPE DYEDPWLPFS HPPTDDAPAL DMSITVGHNC AVQYGVTRED QDEWALRSHQ RAVRAIDAGL FVDEIVPVKV PQADGSTTVF AQDEHPRRDT NRDVLAGLKV LHPEIEGFTV TAGNSSGIND AAAVVALARP GTNHPVLANV LSWATVGVEP KCTGSGPISA ILKTLDLARR KIKDVALFEI NEAFAAQSVA CARELGLDEE IVNVYGSGIS LGHPIAATGA RMVTSAIYEL RRRGGGLGVL SMCAGGGMGA AMLIEVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgcccag cgccgtcgag gcgtcaccaa tcagggtgcc accgggcttc tccgcggccg ggcattcggc agtccgcccc acaccgaagc gttgtggaca tcagaacacg taccgcacga tcaagtcggc gacggcggcc ggcttcacgg atccgtcgat ttcgacggtg tgccgcacga tcaagttcac cgtgtcatcc cccaccttga cggcatcgac gacttccgag cgcactcgga ctcggctacc cgcgatcacc ggagccagaa agcggacctt gttcagcccg tagttgaggc ccattctggc gttttcgacg cggtagttgt ccttgctcaa cccggggatc agcgagagtg tcagaaagcc gtgtgcgatc gtcgcgccga aagggctttg gcttttcgct cgctcgacat ccacgtggat ccactggtga tcgacggtga gctcggcgaa ctggtcgatg cgtgcctggt ccacctcgaa ccaccgactg acaccgagct cctcgcctat cgccttgagc gcctcattga cggaaccgat cgttttcatg caaactcctg ccaaccgcca aatctatatc tatttaacgg tatactctgc aaaagtacca aaggcgcccg tttgggcctg tccgacgacc ccgggagaaa tatgacatcg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggggcggt cgacttcgac gaccttgtgc ttgcggaaag cctgcagggc ggcggggaca gcgcccgcta tatcgctgtc aacctgggcc tgacggcgat tccagggata gccgtcaacc ggcagtgcgc ctcgagcctg tccgcgatcg cgatggctgc agggcaggtg gccgccggaa tgagccgtgc cgtgcttgcc gggggcatgg agtcgtgttc aaccacaccg acaatgcgca agcgcaagcc ctttacctcg ggtacagcgc cggaagacta tgaggacccg tggttgccgt tctcacatcc accgaccgac gacgcgcccg ccctggacat gtcgatcacg gtggggcaca actgcgcagt ccagtacggc gttacccgcg aggaccaaga cgaatgggcg ctgcgcagcc atcagcgcgc ggtgagggca atcgatgccg gcctattcgt cgacgagatc gtgccagtca aggtgccgca ggccgacgga tcgacgaccg tctttgcaca ggacgagcat ccgcgccgcg acaccaaccg agatgtgcta gccgggctaa aggtgcttca cccggaaatc gagggcttca cggtgacggc cgggaactcg tcgggcatca acgatgctgc agctgtggtc gcactggcca ggcccggcac caatcatccc gtcctggcta acgtgctgtc gtgggcgacg gtcggcgtcg aaccgaaatg caccggcagc ggcccgattt cggccattct caagacgctc gacctcgccc gtcgcaagat caaggacgtc gcgctctttg agatcaacga ggcgttcgct gcccagtcgg tggcctgcgc ccgagaactc ggtctcgatg aggagatcgt gaacgtgtac ggatccggta tcagcctggg ccacccgatc gcggccactg gagcgcggat ggtgacgtcg gcaatctacg agttgcgccg ccgcggtggt ggactcggag tcctgtcgat gtgcgcaggc ggcggaatgg gagcggcaat gttaatcgag gtggcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa