MymaA.00829.b
Enoyl-CoA hydratase EchA15
| CENTER ID: | MymaA.00829.b |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00829.b.A1.GE30080 | Full length( MymaA.00829.b ) | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3QK8
DEPOSITED: 1/31/2011
DETERMINATION: XRay
CLONE: MymaA.00829.b.A1.GE30080
PROTEIN: MymaA.00829.b.A1.PS00862
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171365 |
| PATRIC ID: | fig|216594.6.peg.2177 |
| RefSeq: | YP_001850341.1 |
| UniProt: | B2HM22 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLTYQDFPS LRFEPGEHGV LNLVLDSPGL NSVGPQMHRD LADVWPVIDR DPDVRVVLVR GEGKAFSSGG SFELIDETIG DYEGRIRIMR EARDLVLNLV NLDKPVVSAI RGPAVGAGLV VALLADISVA SATAKIIDGH TKLGVAAGDH AAICWPLLVG MAKAKYYLLT CETLSGEEAE RIGLVSTCVD DDEVLPTATR LAENLAQGAQ NAIRWTKRSL NHWYRMFGPT FETSLGLEFL GFTGPDVQEG LAAHRQKRPA RFTDRTEGP
NT Sequence
ATGCCACTCA CCTACCAAGA TTTCCCCAGC CTTCGCTTCG AGCCGGGTGA GCACGGCGTG CTGAATCTGG TGCTGGACTC CCCCGGGCTG AATTCGGTCG GACCGCAGAT GCACCGCGAC CTCGCTGACG TCTGGCCGGT GATCGACCGT GACCCCGACG TGCGGGTGGT GCTGGTTCGT GGTGAGGGCA AAGCGTTTTC GTCCGGGGGC AGTTTTGAGC TCATCGACGA AACGATCGGC GACTACGAAG GCCGAATCCG CATCATGCGC GAGGCCCGTG ACCTGGTGCT CAACCTGGTC AACCTCGACA AGCCGGTGGT TTCGGCGATT CGCGGCCCGG CCGTTGGCGC CGGTCTGGTG GTAGCGCTGC TCGCCGATAT CTCGGTGGCA AGCGCAACCG CGAAAATCAT CGACGGCCAC ACCAAGCTCG GGGTGGCGGC CGGTGACCAC GCCGCGATCT GCTGGCCGCT GCTGGTCGGC ATGGCCAAGG CCAAGTACTA CCTACTCACC TGCGAGACAC TGTCCGGCGA AGAAGCCGAA CGCATCGGCC TGGTCTCGAC GTGCGTCGAT GACGATGAGG TGCTGCCCAC CGCAACCCGG CTGGCCGAGA ATCTGGCGCA GGGGGCGCAA AACGCGATCC GCTGGACCAA GCGCAGCCTC AATCACTGGT ACCGGATGTT CGGGCCGACC TTCGAAACGT CGCTTGGCCT GGAGTTTCTC GGGTTCACCG GCCCCGATGT GCAGGAAGGC CTGGCCGCGC ACCGGCAGAA GCGTCCCGCC CGGTTTACCG ACCGCACCGA GGGGCCC
Details for MymaA.00829.b.A1.GE30080
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPLTYQDFP SLRFEPGEHG VLNLVLDSPG LNSVGPQMHR DLADVWPVID RDPDVRVVLV RGEGKAFSSG GSFELIDETI GDYEGRIRIM REARDLVLNL VNLDKPVVSA IRGPAVGAGL VVALLADISV ASATAKIIDG HTKLGVAAGD HAAICWPLLV GMAKAKYYLL TCETLSGEEA ERIGLVSTCV DDDEVLPTAT RLAENLAQGA QNAIRWTKRS LNHWYRMFGP TFETSLGLEF LGFTGPDVQE GLAAHRQKRP ARFTDRT
Validated NT Sequence
cggtgcggtc ggtaaaccgg gcgggacgct tctgccggtg cgcggccagg ccttcctgca catcggggcc ggtgaacccg agaaactcca ggccaagcga cgtttcgaag gtcggcccga acatccggta ccagtgattg aggctgcgct tggtccagcg gatcgcgttt tgcgccccct gcgccagatt ctcggccagc cgggttgcgg tgggcagcac ctcatcgtca tcgacgcacg tcgagaccag gccgatgcgt tcggcttctt cgccggacag tgtctcgcag gtgagtaggt agtacttggc cttggccatg ccgaccagca gcggccagca gatcgcggcg tggtcaccgg ccgccacccc gagcttggtg tggccgtcga tgattttcgc ggttgcgctt gccaccgaga tatcggcgag cagcgctacc accagaccgg cgccaacggc cgggccgcga atcgccgaaa ccaccggctt gtcgaggttg accaggttga gcaccaggtc acgggcctcg cgcatgatgc ggattcggcc ttcgtagtcg ccgatcgttt cgtcgatgag ctcaaaactg cccccggacg aaaacgcttt gccctcacca cgaaccagca ccacccgcac gtcggggtca cggtcgatca ccggccagac gtcagcgagg tcgcggtgca tctgcggtcc gaccgaattc agcccggggg agtccagcac cagattcagc acgccgtgct cacccggctc gaagcgaagg ctggggaaat cttggtaggt gagtggcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPLTYQDFP SLRFEPGEHG VLNLVLDSPG LNSVGPQMHR DLADVWPVID RDPDVRVVLV RGEGKAFSSG GSFELIDETI GDYEGRIRIM REARDLVLNL VNLDKPVVSA IRGPAVGAGL VVALLADISV ASATAKIIDG HTKLGVAAGD HAAICWPLLV GMAKAKYYLL TCETLSGEEA ERIGLVSTCV DDDEVLPTAT RLAENLAQGA QNAIRWTKRS LNHWYRMFGP TFETSLGLEF LGFTGPDVQE GLAAHRQKRP ARFTDRTEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccactcacc taccaagatt tccccagcct tcgcttcgag ccgggtgagc acggcgtgct gaatctggtg ctggactccc ccgggctgaa ttcggtcgga ccgcagatgc accgcgacct cgctgacgtc tggccggtga tcgaccgtga ccccgacgtg cgggtggtgc tggttcgtgg tgagggcaaa gcgttttcgt ccgggggcag ttttgagctc atcgacgaaa cgatcggcga ctacgaaggc cgaatccgca tcatgcgcga ggcccgtgac ctggtgctca acctggtcaa cctcgacaag ccggtggttt cggcgattcg cggcccggcc gttggcgccg gtctggtggt agcgctgctc gccgatatct cggtggcaag cgcaaccgcg aaaatcatcg acggccacac caagctcggg gtggcggccg gtgaccacgc cgcgatctgc tggccgctgc tggtcggcat ggccaaggcc aagtactacc tactcacctg cgagacactg tccggcgaag aagccgaacg catcggcctg gtctcgacgt gcgtcgatga cgatgaggtg ctgcccaccg caacccggct ggccgagaat ctggcgcagg gggcgcaaaa cgcgatccgc tggaccaagc gcagcctcaa tcactggtac cggatgttcg ggccgacctt cgaaacgtcg cttggcctgg agtttctcgg gttcaccggc cccgatgtgc aggaaggcct ggccgcgcac cggcagaagc gtcccgcccg gtttaccgac cgcaccgagg ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa