MypaA.00829.c
enoyl-CoA hydratase/isomerase
| CENTER ID: | MypaA.00829.c |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00829.c.A1.GE29515 | Full length( MypaA.00829.c ) | 1 | 254 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00829.c.A1.PW29413 | Full length( MypaA.00829.c ) | 1 | 254 |
Structures
3RRV
DEPOSITED: 4/30/2011
DETERMINATION: XRay
CLONE: MypaA.00829.c.A1.GE29515
PROTEIN: MypaA.00829.c.A1.PW29413
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129422 |
| PATRIC ID: | fig|262316.17.peg.718 |
| RefSeq: | NP_959617.1 |
| UniProt: | Q743A2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYDMPTEIDV RADGALRIIT LNRPDSLNSV NDDLHVGLAR LWQRLTDDPT ARAAVITGAG RAFSAGGDFG YLKELSADAD LRAKTIRDGR EIVLGMARCR IPVVAAVNGP AVGLGCSLVA LSDIVYIAEN AYLADPHVQV GLVAADGGPL TWPLHISLLL AKEYALTGTR ISAQRAVELG LANHVADDPV AEAIACAKKI LELPQQAVES TKRVLNIHLE RAVLASLDYA LSAESQSFVT EDFRSIVTKL ADKN
NT Sequence
GTGTACGACA TGCCTACCGA AATCGATGTC CGGGCCGACG GTGCGCTGCG CATCATCACG CTGAACCGGC CGGACTCGCT CAACTCGGTC AACGACGACC TGCACGTGGG GCTGGCGCGG CTGTGGCAGC GGTTGACCGA TGACCCCACC GCGCGTGCCG CCGTGATCAC CGGTGCCGGC CGGGCTTTTT CGGCGGGCGG CGATTTCGGG TATCTCAAGG AGCTGTCCGC CGACGCCGAC CTGCGCGCCA AGACGATCCG GGACGGGCGC GAGATCGTGC TGGGCATGGC GCGCTGCCGG ATTCCGGTGG TAGCGGCCGT CAATGGCCCC GCGGTAGGGC TGGGCTGCAG CCTGGTGGCG CTGAGCGACA TCGTCTACAT CGCCGAGAAC GCCTACCTGG CCGACCCGCA TGTGCAGGTG GGTCTGGTGG CCGCCGACGG TGGGCCGTTG ACCTGGCCGC TGCACATCAG CCTGTTGCTG GCCAAGGAGT ACGCGCTGAC CGGCACCCGG ATCAGCGCGC AGCGCGCCGT CGAGCTCGGG TTGGCCAATC ACGTGGCCGA CGATCCCGTC GCCGAGGCAA TCGCCTGCGC CAAAAAGATT TTGGAGCTGC CGCAGCAGGC GGTGGAAAGC ACCAAGCGGG TGCTCAACAT CCATCTGGAG CGGGCCGTGC TGGCCAGTCT GGACTATGCG TTGTCGGCCG AGAGCCAGTC GTTCGTCACC GAGGATTTCC GGTCGATCGT CACCAAACTG GCGGACAAGA AC
Details for MypaA.00829.c.A1.GE29515
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAK KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLAD
Validated NT Sequence
tgtccgccag tttggtgacg atcgaccgga aatcctcggt gacgaacgac tggctctcgg ccgacaacgc atagtccaga ctggccagca cggcccgctc cagatggatg ttgagcaccc gcttggtgct ttccaccgcc tgctgcggca gctccaaaat ctttttggcg caggcgattg cctcggcgac gggatcgtcg gccacgtgat tggccaaccc gagctcgacg gcgcgctgcg cgctgatccg ggtgccggtc agcgcgtact ccttggccag caacaggctg atgtgcagcg gccaggtcaa cggcccaccg tcggcggcca ccagacccac ctgcacatgc gggtcggcca ggtaggcgtt ctcggcgatg tagacgatgt cgctcagcgc caccaggctg cagcccagcc ctaccgcggg gccattgacg gccgctacca ccggaatccg gcagcgcgcc atgcccagca cgatctcgcg cccgtcccgg atcgtcttgg cgcgcaggtc ggcgtcggcg gacagctcct tgagataccc gaaatcgccg cccgccgaaa aagcccggcc ggcaccggtg atcacggcgg cacgcgcggt ggggtcatcg gtcaaccgct gccacagccg cgccagcccc acgtgcaggt cgtcgttgac cgagttgagc gagtccggcc ggttcagcgt gatgatgcgc agcgcaccgt cggcccggac atcgatttcg gtaggcatgt cgtacaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAK KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLADKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacgac atgcctaccg aaatcgatgt ccgggccgac ggtgcgctgc gcatcatcac gctgaaccgg ccggactcgc tcaactcggt caacgacgac ctgcacgtgg ggctggcgcg gctgtggcag cggttgaccg atgaccccac cgcgcgtgcc gccgtgatca ccggtgccgg ccgggctttt tcggcgggcg gcgatttcgg gtatctcaag gagctgtccg ccgacgccga cctgcgcgcc aagacgatcc gggacgggcg cgagatcgtg ctgggcatgg cgcgctgccg gattccggtg gtagcggccg tcaatggccc cgcggtaggg ctgggctgca gcctggtggc gctgagcgac atcgtctaca tcgccgagaa cgcctacctg gccgacccgc atgtgcaggt gggtctggtg gccgccgacg gtgggccgtt gacctggccg ctgcacatca gcctgttgct ggccaaggag tacgcgctga ccggcacccg gatcagcgcg cagcgcgccg tcgagctcgg gttggccaat cacgtggccg acgatcccgt cgccgaggca atcgcctgcg ccaaaaagat tttggagctg ccgcagcagg cggtggaaag caccaagcgg gtgctcaaca tccatctgga gcgggccgtg ctggccagtc tggactatgc gttgtcggcc gagagccagt cgttcgtcac cgaggatttc cggtcgatcg tcaccaaact ggcggacaag aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MypaA.00829.c.A1.PW29413
| PURIFICATION DATe: | 1/14/2011 |
| CONCENTRATION: | 25.67mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAK KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLAD
Validated NT Sequence
tgtccgccag tttggtgacg atcgaccgga aatcctcggt gacgaacgac tggctctcgg ccgacaacgc atagtccaga ctggccagca cggcccgctc cagatggatg ttgagcaccc gcttggtgct ttccaccgcc tgctgcggca gctccaaaat ctttttggcg caggcgattg cctcggcgac gggatcgtcg gccacgtgat tggccaaccc gagctcgacg gcgcgctgcg cgctgatccg ggtgccggtc agcgcgtact ccttggccag caacaggctg atgtgcagcg gccaggtcaa cggcccaccg tcggcggcca ccagacccac ctgcacatgc gggtcggcca ggtaggcgtt ctcggcgatg tagacgatgt cgctcagcgc caccaggctg cagcccagcc ctaccgcggg gccattgacg gccgctacca ccggaatccg gcagcgcgcc atgcccagca cgatctcgcg cccgtcccgg atcgtcttgg cgcgcaggtc ggcgtcggcg gacagctcct tgagataccc gaaatcgccg cccgccgaaa aagcccggcc ggcaccggtg atcacggcgg cacgcgcggt ggggtcatcg gtcaaccgct gccacagccg cgccagcccc acgtgcaggt cgtcgttgac cgagttgagc gagtccggcc ggttcagcgt gatgatgcgc agcgcaccgt cggcccggac atcgatttcg gtaggcatgt cgtacaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAK KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLADKN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTACGAC ATGCCTACCG AAATCGATGT CCGGGCCGAC GGTGCGCTGC GCATCATCAC GCTGAACCGG CCGGACTCGC TCAACTCGGT CAACGACGAC CTGCACGTGG GGCTGGCGCG GCTGTGGCAG CGGTTGACCG ATGACCCCAC CGCGCGTGCC GCCGTGATCA CCGGTGCCGG CCGGGCTTTT TCGGCGGGCG GCGATTTCGG GTATCTCAAG GAGCTGTCCG CCGACGCCGA CCTGCGCGCC AAGACGATCC GGGACGGGCG CGAGATCGTG CTGGGCATGG CGCGCTGCCG GATTCCGGTG GTAGCGGCCG TCAATGGCCC CGCGGTAGGG CTGGGCTGCA GCCTGGTGGC GCTGAGCGAC ATCGTCTACA TCGCCGAGAA CGCCTACCTG GCCGACCCGC ATGTGCAGGT GGGTCTGGTG GCCGCCGACG GTGGGCCGTT GACCTGGCCG CTGCACATCA GCCTGTTGCT GGCCAAGGAG TACGCGCTGA CCGGCACCCG GATCAGCGCG CAGCGCGCCG TCGAGCTCGG GTTGGCCAAT CACGTGGCCG ACGATCCCGT CGCCGAGGCA ATCGCCTGCG CCAAAAAGAT TTTGGAGCTG CCGCAGCAGG CGGTGGAAAG CACCAAGCGG GTGCTCAACA TCCATCTGGA GCGGGCCGTG CTGGCCAGTC TGGACTATGC GTTGTCGGCC GAGAGCCAGT CGTTCGTCAC CGAGGATTTC CGGTCGATCG TCACCAAACT GGCGGACAAG AACAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA