MyavA.00829.a

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MyavA.00829.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00829.a.A1.GE29027 Full length( MyavA.00829.a ) 1 254
MyavA.00829.a.AE1.GE43231 Full length( MyavA.00829.a ) 1 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00829.a.A1.PW28932 Full length( MyavA.00829.a ) 1 254
MyavA.00829.a.AE1.PW38719 Full length( MyavA.00829.a ) 1 254

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.836
RefSeq: YP_880135.1
UniProt: A0A0H2ZT88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYDMPTEIDV RADGALRIIT LNRPDSLNSV NDDLHVGLAR LWQRLTDDPT ARAAVITGAG RAFSAGGDFG YLKELSADAD LRAKTIRDGR EIVLGMARCR IPVVAAVNGP AVGLGCSLVA LSDIVYIAEN AYLADPHVQV GLVAADGGPL TWPLHISLLL AKEYALTGTR ISAQRAVELG LANHVADDPV AEAIACAQKI LELPQQAVES TKRVLNIHLE RAVLASLDYA LSAESQSFVT EDFRSIVTKL ADKN
NT Sequence
GTGTACGACA TGCCTACCGA AATCGATGTC CGGGCCGACG GTGCGCTGCG CATCATCACG CTGAACCGGC CGGACTCGCT CAACTCGGTC AACGACGACC TGCACGTGGG GCTGGCGCGG CTGTGGCAGC GGTTGACCGA TGACCCCACC GCGCGTGCCG CCGTGATCAC CGGTGCCGGC CGGGCTTTTT CGGCGGGCGG CGATTTCGGG TATCTCAAGG AGTTGTCCGC CGACGCCGAC CTGCGTGCCA AGACCATCCG GGACGGGCGC GAGATCGTGC TGGGCATGGC GCGCTGCCGG ATTCCGGTGG TGGCGGCCGT CAATGGCCCC GCGGTAGGGC TGGGCTGCAG CCTGGTGGCG CTGAGCGACA TCGTCTACAT CGCCGAGAAC GCCTACCTGG CCGACCCGCA TGTACAGGTG GGTCTGGTGG CCGCCGACGG TGGGCCGTTG ACCTGGCCGC TGCACATCAG CCTGTTGCTG GCCAAGGAGT ACGCGCTGAC CGGCACCCGG ATCAGCGCGC AGCGCGCCGT CGAGCTCGGG TTGGCCAATC ACGTGGCCGA CGATCCCGTC GCCGAGGCAA TCGCCTGCGC CCAAAAGATT TTGGAGCTGC CGCAGCAGGC GGTGGAAAGC ACCAAGCGGG TGCTCAACAT CCATCTGGAG CGGGCCGTGC TGGCCAGTCT GGACTATGCG TTGTCGGCCG AGAGCCAGTC GTTCGTCACC GAGGATTTCC GGTCGATCGT CACCAAACTG GCGGACAAGA AC
Details for MyavA.00829.a.A1.GE29027
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAQ KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLADKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacgac atgcctaccg aaatcgatgt ccgggccgac ggtgcgctgc gcatcatcac gctgaaccgg ccggactcgc tcaactcggt caacgacgac ctgcacgtgg ggctggcgcg gctgtggcag cggttgaccg atgaccccac cgcgcgtgcc gccgtgatca ccggtgccgg ccgggctttt tcggcgggcg gcgatttcgg gtatctcaag gagttgtccg ccgacgccga cctgcgtgcc aagaccatcc gggacgggcg cgagatcgtg ctgggcatgg cgcgctgccg gattccggtg gtggcggccg tcaatggccc cgcggtaggg ctgggctgca gcctggtggc gctgagcgac atcgtctaca tcgccgagaa cgcctacctg gccgacccgc atgtacaggt gggtctggtg gccgccgacg gtgggccgtt gacctggccg ctgcacatca gcctgttgct ggccaaggag tacgcgctga ccggcacccg gatcagcgcg cagcgcgccg tcgagctcgg gttggccaat cacgtggccg acgatcccgt cgccgaggca atcgcctgcg cccaaaagat tttggagctg ccgcagcagg cggtggaaag caccaagcgg gtgctcaaca tccatctgga gcgggccgtg ctggccagtc tggactatgc gttgtcggcc gagagccagt cgttcgtcac cgaggatttc cggtcgatcg tcaccaaact ggcggacaag aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyavA.00829.a.AE1.GE43231
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 7
PERCENT COVERAGE: 117
Validated AA Sequence
MXSNDSXRXV LRLHLRPXXX PPRRRTRRDA RAGARVGHLH LSAPPGRAAR XXKXXXGXXA SCSWXGVCGP AXSRADRXHP SRCPGXXSRR TRXXXXRXXS XSXXGXXXXX XXIRTXXPCE XXXIVXXSRW IAVSRXXXXX XXXXGXXXXX CWXXXPXXXD GSXXXXPTXX XXPPFLXGXX LXXPXXXXXX TXXXXXXXXV XXGXXXPPXX XXXXXXRQXX GXXXXXXXXX XXXXXKTXGX XXXXXXXXXY XXXXXXXXXX XXXXXXXXXP XXXXXXXXXP XXXXXXXXXX XXXXXGXXXX XXXXXXX
Validated NT Sequence
tnctttcgng ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga gctcgaattc ggatccgtta atgatgatga tggtggtgcc cagctctgtc cggatccggg tggangacga gcnnnggatc ctgctcgtgg nncngcgact nnnnnttgcc tactncgccn ngtccagacc ggccgtctcg acccgntnca ggaggatgta gagcaccncc ttgatgattt ccaccgcctg ctgggnnttn tncatcaagt ntttggnnan acgatggcct cggtgangac gacgatttcc catgtgntcg aatgactcga gncgagnggt tctgcgcctg catctgcgac ccngcatnng acctcctcgg cggcgaacac ggcgagatgc ccgtgcaggt gcacgagtgg gacatctgca cctatcagct ccacctgggc gtgccgcccg tcnnnacaag nnccnccngg gagannaagc aagttgctca tggnccggtg tgtgcggccc agccncatcg cgggcngata ggcnccaccc atcccgntgc ccgggcngcn aatcacgtcg aactcgatgn cntnnangta ganaatgntc ttngagtnan nntggnntcn nccannnnnc antgagnatc cgcacagnnn ccccctgcga gnnnnnnnag attgtcntgn natcnagatg gatcgcngtg tcgcggncnc nnancnncnc tgncnnnnnc ncgggnnccc nnnggnggng gtgctggntg nnatntcccc nntannaaga tgggtcaann tgnngntanc ccacctgnan nntncncccc ccgttccttn anggnnntna gctctnaang cccnnnnnnn nnnncnnnnn taccgnntnn cnnntcnacn tctnnanggt gncnganggg gntnnnantc ctccangnng annngnanga tntnnnngaa gacagcnntn ggggancntc ntnngnnnnn nnngncnnng gncnanatnt nnnnnnaaaa cnnntgggtn nagnnntann nnancnnnnn tgcnnnanta cttnnnnngn nnntnnnann nnnnnnnnnn nncnagnntn nnanannnnn nnnnnnnncc nnnntncntn nnnnnnnnnn nncnnntncc gngnnnnnnn nnnnnnnnnn nntnnnnnga nnannnnnnn gngannggga nnnnnncnnn nnnnnnnnnn nnntnnnnnt nnnc
Expected Protein Sequence
MYDMPTEIDV RADGALRIIT LNRPDSLNSV NDDLHVGLAR LWQRLTDDPT ARAAVITGAG RAFSAGGDFG YLKELSADAD LRAKTIRDGR EIVLGMARCR IPVVAAVNGP AVGLGCSLVA LSDIVYIAEN AYLADPHVQV GLVAADGGPL TWPLHISLLL AKEYALTGTR ISAQRAVELG LANHVADDPV AEAIACAQKI LELPQQAVES TKRVLNIHLE RAVLASLDYA LSAESQSFVT EDFRSIVTKL ADKNGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gtacgacatg cctaccgaaa tcgatgtccg ggccgacggt gcgctgcgca tcatcacgct gaaccggccg gactcgctca actcggtcaa cgacgacctg cacgtggggc tggcgcggct gtggcagcgg ttgaccgatg accccaccgc gcgtgccgcc gtgatcaccg gtgccggccg ggctttttcg gcgggcggcg atttcgggta tctcaaggag ttgtccgccg acgccgacct gcgtgccaag accatccggg acgggcgcga gatcgtgctg ggcatggcgc gctgccggat tccggtggtg gcggccgtca atggccccgc ggtagggctg ggctgcagcc tggtggcgct gagcgacatc gtctacatcg ccgagaacgc ctacctggcc gacccgcatg tacaggtggg tctggtggcc gccgacggtg ggccgttgac ctggccgctg cacatcagcc tgttgctggc caaggagtac gcgctgaccg gcacccggat cagcgcgcag cgcgccgtcg agctcgggtt ggccaatcac gtggccgacg atcccgtcgc cgaggcaatc gcctgcgccc aaaagatttt ggagctgccg cagcaggcgg tggaaagcac caagcgggtg ctcaacatcc atctggagcg ggccgtgctg gccagtctgg actatgcgtt gtcggccgag agccagtcgt tcgtcaccga ggatttccgg tcgatcgtca ccaaactggc ggacaagaac gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for MyavA.00829.a.A1.PW28932
PURIFICATION DATe: 7/12/2010
CONCENTRATION: 25.93mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYDMPTEI DVRADGALRI ITLNRPDSLN SVNDDLHVGL ARLWQRLTDD PTARAAVITG AGRAFSAGGD FGYLKELSAD ADLRAKTIRD GREIVLGMAR CRIPVVAAVN GPAVGLGCSL VALSDIVYIA ENAYLADPHV QVGLVAADGG PLTWPLHISL LLAKEYALTG TRISAQRAVE LGLANHVADD PVAEAIACAQ KILELPQQAV ESTKRVLNIH LERAVLASLD YALSAESQSF VTEDFRSIVT KLADKN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTACGAC ATGCCTACCG AAATCGATGT CCGGGCCGAC GGTGCGCTGC GCATCATCAC GCTGAACCGG CCGGACTCGC TCAACTCGGT CAACGACGAC CTGCACGTGG GGCTGGCGCG GCTGTGGCAG CGGTTGACCG ATGACCCCAC CGCGCGTGCC GCCGTGATCA CCGGTGCCGG CCGGGCTTTT TCGGCGGGCG GCGATTTCGG GTATCTCAAG GAGTTGTCCG CCGACGCCGA CCTGCGTGCC AAGACCATCC GGGACGGGCG CGAGATCGTG CTGGGCATGG CGCGCTGCCG GATTCCGGTG GTGGCGGCCG TCAATGGCCC CGCGGTAGGG CTGGGCTGCA GCCTGGTGGC GCTGAGCGAC ATCGTCTACA TCGCCGAGAA CGCCTACCTG GCCGACCCGC ATGTACAGGT GGGTCTGGTG GCCGCCGACG GTGGGCCGTT GACCTGGCCG CTGCACATCA GCCTGTTGCT GGCCAAGGAG TACGCGCTGA CCGGCACCCG GATCAGCGCG CAGCGCGCCG TCGAGCTCGG GTTGGCCAAT CACGTGGCCG ACGATCCCGT CGCCGAGGCA ATCGCCTGCG CCCAAAAGAT TTTGGAGCTG CCGCAGCAGG CGGTGGAAAG CACCAAGCGG GTGCTCAACA TCCATCTGGA GCGGGCCGTG CTGGCCAGTC TGGACTATGC GTTGTCGGCC GAGAGCCAGT CGTTCGTCAC CGAGGATTTC CGGTCGATCG TCACCAAACT GGCGGACAAG AACAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for MyavA.00829.a.AE1.PW38719
PURIFICATION DATe: 10/16/2019
CONCENTRATION: 32.5mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 7
PERCENT COVERAGE: 117
Protocol Notes
notes unavailable
Validated AA Sequence
MXSNDSXRXV LRLHLRPXXX PPRRRTRRDA RAGARVGHLH LSAPPGRAAR XXKXXXGXXA SCSWXGVCGP AXSRADRXHP SRCPGXXSRR TRXXXXRXXS XSXXGXXXXX XXIRTXXPCE XXXIVXXSRW IAVSRXXXXX XXXXGXXXXX CWXXXPXXXD GSXXXXPTXX XXPPFLXGXX LXXPXXXXXX TXXXXXXXXV XXGXXXPPXX XXXXXXRQXX GXXXXXXXXX XXXXXKTXGX XXXXXXXXXY XXXXXXXXXX XXXXXXXXXP XXXXXXXXXP XXXXXXXXXX XXXXXGXXXX XXXXXXX
Validated NT Sequence
tnctttcgng ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga gctcgaattc ggatccgtta atgatgatga tggtggtgcc cagctctgtc cggatccggg tggangacga gcnnnggatc ctgctcgtgg nncngcgact nnnnnttgcc tactncgccn ngtccagacc ggccgtctcg acccgntnca ggaggatgta gagcaccncc ttgatgattt ccaccgcctg ctgggnnttn tncatcaagt ntttggnnan acgatggcct cggtgangac gacgatttcc catgtgntcg aatgactcga gncgagnggt tctgcgcctg catctgcgac ccngcatnng acctcctcgg cggcgaacac ggcgagatgc ccgtgcaggt gcacgagtgg gacatctgca cctatcagct ccacctgggc gtgccgcccg tcnnnacaag nnccnccngg gagannaagc aagttgctca tggnccggtg tgtgcggccc agccncatcg cgggcngata ggcnccaccc atcccgntgc ccgggcngcn aatcacgtcg aactcgatgn cntnnangta ganaatgntc ttngagtnan nntggnntcn nccannnnnc antgagnatc cgcacagnnn ccccctgcga gnnnnnnnag attgtcntgn natcnagatg gatcgcngtg tcgcggncnc nnancnncnc tgncnnnnnc ncgggnnccc nnnggnggng gtgctggntg nnatntcccc nntannaaga tgggtcaann tgnngntanc ccacctgnan nntncncccc ccgttccttn anggnnntna gctctnaang cccnnnnnnn nnnncnnnnn taccgnntnn cnnntcnacn tctnnanggt gncnganggg gntnnnantc ctccangnng annngnanga tntnnnngaa gacagcnntn ggggancntc ntnngnnnnn nnngncnnng gncnanatnt nnnnnnaaaa cnnntgggtn nagnnntann nnancnnnnn tgcnnnanta cttnnnnngn nnntnnnann nnnnnnnnnn nncnagnntn nnanannnnn nnnnnnnncc nnnntncntn nnnnnnnnnn nncnnntncc gngnnnnnnn nnnnnnnnnn nntnnnnnga nnannnnnnn gngannggga nnnnnncnnn nnnnnnnnnn nnntnnnnnt nnnc
Expressed Protein Sequence
MYDMPTEIDV RADGALRIIT LNRPDSLNSV NDDLHVGLAR LWQRLTDDPT ARAAVITGAG RAFSAGGDFG YLKELSADAD LRAKTIRDGR EIVLGMARCR IPVVAAVNGP AVGLGCSLVA LSDIVYIAEN AYLADPHVQV GLVAADGGPL TWPLHISLLL AKEYALTGTR ISAQRAVELG LANHVADDPV AEAIACAQKI LELPQQAVES TKRVLNIHLE RAVLASLDYA LSAESQSFVT EDFRSIVTKL ADKNGHHHHH H
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCGT GTACGACATG CCTACCGAAA TCGATGTCCG GGCCGACGGT GCGCTGCGCA TCATCACGCT GAACCGGCCG GACTCGCTCA ACTCGGTCAA CGACGACCTG CACGTGGGGC TGGCGCGGCT GTGGCAGCGG TTGACCGATG ACCCCACCGC GCGTGCCGCC GTGATCACCG GTGCCGGCCG GGCTTTTTCG GCGGGCGGCG ATTTCGGGTA TCTCAAGGAG TTGTCCGCCG ACGCCGACCT GCGTGCCAAG ACCATCCGGG ACGGGCGCGA GATCGTGCTG GGCATGGCGC GCTGCCGGAT TCCGGTGGTG GCGGCCGTCA ATGGCCCCGC GGTAGGGCTG GGCTGCAGCC TGGTGGCGCT GAGCGACATC GTCTACATCG CCGAGAACGC CTACCTGGCC GACCCGCATG TACAGGTGGG TCTGGTGGCC GCCGACGGTG GGCCGTTGAC CTGGCCGCTG CACATCAGCC TGTTGCTGGC CAAGGAGTAC GCGCTGACCG GCACCCGGAT CAGCGCGCAG CGCGCCGTCG AGCTCGGGTT GGCCAATCAC GTGGCCGACG ATCCCGTCGC CGAGGCAATC GCCTGCGCCC AAAAGATTTT GGAGCTGCCG CAGCAGGCGG TGGAAAGCAC CAAGCGGGTG CTCAACATCC ATCTGGAGCG GGCCGTGCTG GCCAGTCTGG ACTATGCGTT GTCGGCCGAG AGCCAGTCGT TCGTCACCGA GGATTTCCGG TCGATCGTCA CCAAACTGGC GGACAAGAAC GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT