MypaA.00829.b
EchA15
| CENTER ID: | MypaA.00829.b |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00829.b.A1.GE29514 | Full length( MypaA.00829.b ) | 1 | 273 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7M3W
DEPOSITED: 3/19/2021
DETERMINATION: XRay
CLONE: MypaA.00829.b.A1.GE29514
PROTEIN: MypaA.00829.b.A1.PS00744
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|262316.17.peg.2966 |
| RefSeq: | NP_961734.1 |
| UniProt: | Q73W60 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVSHPPVDY HDFPSLRCEL GDDGVLTVVL DSPGLNSVGP QMHRDLADIW PVIDRDPAVR AVLVRGEGKA FSSGGSFDLI DETIGDYQGR VRIMREARDL VHNMINCDTP VVSAIRGPAV GAGLVVALLA DISVAGRTAK LIDGHTKLGV AAGDHAAICW PLLVGMAKAK YYLLTCETLL GEEAERIGLV SLCVDDDDVL STAAGIAGKL AQGAQHAIQW TKRSLNHWYR MMGPTFETSV GLEFLSFSGP DVQEGLAAHR EKRAARFTGG SAT
NT Sequence
GTGCCAGTCA GCCACCCGCC GGTCGATTAC CACGACTTCC CCAGCCTGCG CTGCGAACTC GGCGACGACG GGGTGCTGAC GGTGGTGCTC GACTCGCCGG GTCTGAATTC GGTGGGGCCG CAGATGCACC GCGACCTGGC CGACATCTGG CCGGTGATCG ACCGCGACCC GGCGGTGCGC GCGGTGCTGG TCCGCGGTGA GGGCAAGGCC TTCTCCTCCG GCGGCAGCTT CGATTTGATC GACGAGACCA TCGGCGACTA CCAGGGCCGG GTCCGCATCA TGCGGGAGGC GCGCGACCTG GTGCACAACA TGATCAACTG CGACACCCCG GTGGTGTCGG CGATCCGCGG GCCGGCCGTG GGCGCGGGCC TGGTGGTGGC CCTGCTCGCC GACATCTCGG TGGCCGGCCG CACCGCCAAG CTGATCGACG GGCACACCAA GCTGGGCGTG GCCGCCGGCG ACCACGCCGC CATCTGCTGG CCGCTGCTGG TCGGCATGGC CAAGGCCAAG TACTACCTGC TGACCTGCGA GACGCTGCTC GGCGAGGAGG CCGAGCGGAT CGGCCTGGTG TCGCTGTGCG TCGACGACGA CGACGTGCTG TCCACCGCCG CGGGCATCGC CGGGAAACTG GCGCAGGGCG CCCAGCACGC CATCCAATGG ACCAAGCGCA GCCTCAACCA CTGGTACCGC ATGATGGGCC CCACCTTCGA GACCTCGGTG GGGCTGGAAT TCCTCAGTTT CAGCGGGCCC GACGTGCAGG AAGGCCTGGC CGCGCACCGG GAGAAACGCG CCGCCCGCTT CACCGGCGGA TCTGCCACC
Details for MypaA.00829.b.A1.GE29514
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVPVSHP PVDYHDFPSL RCELGDDGVL TVVLDSPGLN SVGPQMHRDL ADIWPVIDRD PAVRAVLVRG EGKAFSSGGS FDLIDETIGD YQGRVRIMRE ARDLVHNMIN CDTPVVSAIR GPAVGAGLVV ALLADISVAG RTAKLIDGHT KLGVAAGDHA AICWPLLVGM AKAKYYLLTC ETLLGEEAER IGLVSLCVDD DDVLSTAAGI AGKLAQGAQH AIQWTKRSLN HWYRMMGPTF ETSVGLEFLS FSGPDVQEGL AAHREKRAAR FTGGSAT
Validated NT Sequence
ttattaggtg gcagatccgc cggtgaagcg ggcggcgcgt ttctcccggt gcgcggccag gccttcctgc acgtcgggcc cgctgaaact gaggaattcc agccccaccg aggtctcgaa ggtggggccc atcatgcggt accagtggtt gaggctgcgc ttggtccatt ggatggcgtg ctgggcgccc tgcgccagtt tcccggcgat gcccgcggcg gtggacagca cgtcgtcgtc gtcgacgcac agcgacacca ggccgatccg ctcggcctcc tcgccgagca gcgtctcgca ggtcagcagg tagtacttgg ccttggccat gccgaccagc agcggccagc agatggcggc gtggtcgccg gcggccacgc ccagcttggt gtgcccgtcg atcagcttgg cggtgcggcc ggccaccgag atgtcggcga gcagggccac caccaggccc gcgcccacgg ccggcccgcg gatcgccgac accaccgggg tgtcgcagtt gatcatgttg tgcaccaggt cgcgcgcctc ccgcatgatg cggacccggc cctggtagtc gccgatggtc tcgtcgatca aatcgaagct gccgccggag gagaaggcct tgccctcacc gcggaccagc accgcgcgca ccgccgggtc gcggtcgatc accggccaga tgtcggccag gtcgcggtgc atctgcggcc ccaccgaatt cagacccggc gagtcgagca ccaccgtcag caccccgtcg tcgccgagtt cgcagcgcag gctggggaag tcgtggtaat cgaccggcgg gtggctgact ggcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSHPPV DYHDFPSLRC ELGDDGVLTV VLDSPGLNSV GPQMHRDLAD IWPVIDRDPA VRAVLVRGEG KAFSSGGSFD LIDETIGDYQ GRVRIMREAR DLVHNMINCD TPVVSAIRGP AVGAGLVVAL LADISVAGRT AKLIDGHTKL GVAAGDHAAI CWPLLVGMAK AKYYLLTCET LLGEEAERIG LVSLCVDDDD VLSTAAGIAG KLAQGAQHAI QWTKRSLNHW YRMMGPTFET SVGLEFLSFS GPDVQEGLAA HREKRAARFT GGSAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccagtc agccacccgc cggtcgatta ccacgacttc cccagcctgc gctgcgaact cggcgacgac ggggtgctga cggtggtgct cgactcgccg ggtctgaatt cggtggggcc gcagatgcac cgcgacctgg ccgacatctg gccggtgatc gaccgcgacc cggcggtgcg cgcggtgctg gtccgcggtg agggcaaggc cttctcctcc ggcggcagct tcgatttgat cgacgagacc atcggcgact accagggccg ggtccgcatc atgcgggagg cgcgcgacct ggtgcacaac atgatcaact gcgacacccc ggtggtgtcg gcgatccgcg ggccggccgt gggcgcgggc ctggtggtgg ccctgctcgc cgacatctcg gtggccggcc gcaccgccaa gctgatcgac gggcacacca agctgggcgt ggccgccggc gaccacgccg ccatctgctg gccgctgctg gtcggcatgg ccaaggccaa gtactacctg ctgacctgcg agacgctgct cggcgaggag gccgagcgga tcggcctggt gtcgctgtgc gtcgacgacg acgacgtgct gtccaccgcc gcgggcatcg ccgggaaact ggcgcagggc gcccagcacg ccatccaatg gaccaagcgc agcctcaacc actggtaccg catgatgggc cccaccttcg agacctcggt ggggctggaa ttcctcagtt tcagcgggcc cgacgtgcag gaaggcctgg ccgcgcaccg ggagaaacgc gccgcccgct tcaccggcgg atctgccacc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa