MyavA.00829.e
Enoyl-CoA hydratase
| CENTER ID: | MyavA.00829.e |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00829.e.A1.GE29031 | Full length( MyavA.00829.e ) | 1 | 273 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00829.e.A1.PW28936 | Full length( MyavA.00829.e ) | 1 | 273 |
Structures
3Q1T
DEPOSITED: 12/17/2010
DETERMINATION: XRay
CLONE: MyavA.00829.e.A1.GE29031
PROTEIN: MyavA.00829.e.A1.PW28936
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171365 |
| PATRIC ID: | fig|243243.7.peg.3494 |
| RefSeq: | YP_882751.1 |
| UniProt: | A0A0H2ZRU2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVSHPPVDY HDFPSLRCEL GDDGVLTVVL DSPGLNSVGP QMHRDLADIW PVIDRDPAVR AVLVRGEGKA FSSGGSFDLI DETIGDYQGR VRIMREARDL VHNMINCDTP VVSAIRGPAV GAGLVVALLA DISVAGRTAK LIDGHTKLGV AAGDHAAICW PLLVGMAKAK YYLLTCETLL GEEAERIGLV SLCVDDDDVL STAAGIAGKL AQGAQHAIQW TKRSLNHWYR TMGPTFETSV GLEFLSFSGP DVQEGLAAHR EKRAARFTGG SAT
NT Sequence
GTGCCAGTCA GCCACCCGCC GGTCGATTAC CACGACTTCC CCAGCCTGCG CTGCGAACTC GGCGACGACG GGGTGCTGAC GGTGGTGCTC GACTCGCCGG GTCTGAATTC GGTGGGGCCG CAGATGCACC GCGACCTGGC CGACATCTGG CCGGTGATCG ACCGCGACCC GGCGGTGCGC GCGGTGCTGG TCCGCGGTGA GGGCAAGGCC TTCTCCTCCG GCGGCAGCTT CGACCTGATC GACGAGACCA TCGGCGACTA CCAGGGCCGG GTCCGCATCA TGCGGGAAGC CCGCGACCTG GTGCACAACA TGATCAACTG CGACACCCCG GTGGTGTCGG CGATCCGCGG GCCGGCCGTG GGCGCGGGCC TGGTGGTGGC CCTGCTCGCC GACATCTCGG TGGCCGGCCG CACCGCCAAG CTGATCGACG GGCACACCAA GCTGGGCGTG GCCGCCGGCG ACCACGCCGC CATCTGCTGG CCGCTGCTGG TCGGCATGGC CAAGGCCAAG TACTACCTGC TGACCTGCGA GACGCTGCTC GGCGAGGAGG CCGAGCGGAT CGGCCTGGTG TCGCTGTGCG TCGACGACGA CGACGTGCTG TCCACCGCCG CGGGCATCGC CGGGAAACTG GCGCAGGGCG CCCAGCACGC CATCCAATGG ACCAAGCGCA GCCTCAACCA CTGGTACCGC ACGATGGGCC CCACCTTCGA GACCTCGGTG GGGCTGGAAT TCCTCAGTTT CAGCGGGCCC GACGTGCAGG AAGGCCTGGC CGCGCACCGG GAGAAACGCG CCGCCCGCTT CACCGGCGGA TCTGCCACC
Details for MyavA.00829.e.A1.GE29031
| HARVESTED ON: | 4/9/2010 |
| SEQUENCED ON: | 4/29/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSHPPV DYHDFPSLRC ELGDDGVLTV VLDSPGLNSV GPQMHRDLAD IWPVIDRDPA VRAVLVRGEG KAFSSGGSFD LIDETIGDYQ GRIRIMREAR DLVHNMINCD TPVVSAIRGP AVGAGLVVAL LADISVAGRT AKLIDGHTKL GVAAGDHAAI CWPLLVGMAK AKYYLLTCET LLGEEAERIG LVSLCVDDDD VLSTAAGIAG KLAQGAQHAI QWTKRSLNHW YRMMGPTFET SVGLEFLSFS GPDVQEGLAA HREKRAARFT GGSAT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc cagtcagcca cccgccggtc gattaccacg acttccccag cctgcgctgc gaactcggcg acgacggggt gctgacggtg gtgctcgact cgccgggtct gaattcggtg gggccgcaga tgcaccgcga cctggccgac atctggccgg tgatcgaccg cgacccggcg gtgcgcgcgg tgctggtccg cggtgagggc aaggccttct cctccggcgg cagcttcgac ctgatcgacg agaccatcgg cgattaccag ggccggatcc gcatcatgcg ggaggcccgc gacctagtgc acaacatgat caactgcgac accccggtgg tgtcggcgat ccgcgggccg gccgtgggcg cgggcctggt ggtggccctg ctcgccgaca tctcggtggc cggccgcacc gccaagctga tcgacgggca caccaagctg ggcgtggccg ccggcgacca cgccgccatc tgctggccgc tgctggtcgg catggccaag gccaagtact acctgctgac ctgcgagacg ctgctcggcg aggaggctga gcggatcggc ctggtgtcgc tgtgcgtcga cgacgacgac gtgctgtcca ccgccgcggg catcgccggg aaactggcgc agggcgccca gcacgccatc caatggacca agcgcagcct caatcactgg taccgcatga tgggccccac cttcgagacc tcggtggggc tggaattcct cagtttcagc gggcccgacg tgcaggaagg cctggccgcg caccgggaaa aacgcgccgc ccgcttcacc ggcggatctg ccacctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSHPPV DYHDFPSLRC ELGDDGVLTV VLDSPGLNSV GPQMHRDLAD IWPVIDRDPA VRAVLVRGEG KAFSSGGSFD LIDETIGDYQ GRVRIMREAR DLVHNMINCD TPVVSAIRGP AVGAGLVVAL LADISVAGRT AKLIDGHTKL GVAAGDHAAI CWPLLVGMAK AKYYLLTCET LLGEEAERIG LVSLCVDDDD VLSTAAGIAG KLAQGAQHAI QWTKRSLNHW YRTMGPTFET SVGLEFLSFS GPDVQEGLAA HREKRAARFT GGSAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccagtc agccacccgc cggtcgatta ccacgacttc cccagcctgc gctgcgaact cggcgacgac ggggtgctga cggtggtgct cgactcgccg ggtctgaatt cggtggggcc gcagatgcac cgcgacctgg ccgacatctg gccggtgatc gaccgcgacc cggcggtgcg cgcggtgctg gtccgcggtg agggcaaggc cttctcctcc ggcggcagct tcgacctgat cgacgagacc atcggcgact accagggccg ggtccgcatc atgcgggaag cccgcgacct ggtgcacaac atgatcaact gcgacacccc ggtggtgtcg gcgatccgcg ggccggccgt gggcgcgggc ctggtggtgg ccctgctcgc cgacatctcg gtggccggcc gcaccgccaa gctgatcgac gggcacacca agctgggcgt ggccgccggc gaccacgccg ccatctgctg gccgctgctg gtcggcatgg ccaaggccaa gtactacctg ctgacctgcg agacgctgct cggcgaggag gccgagcgga tcggcctggt gtcgctgtgc gtcgacgacg acgacgtgct gtccaccgcc gcgggcatcg ccgggaaact ggcgcagggc gcccagcacg ccatccaatg gaccaagcgc agcctcaacc actggtaccg cacgatgggc cccaccttcg agacctcggt ggggctggaa ttcctcagtt tcagcgggcc cgacgtgcag gaaggcctgg ccgcgcaccg ggagaaacgc gccgcccgct tcaccggcgg atctgccacc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyavA.00829.e.A1.PW28936
| PURIFICATION DATe: | 7/12/2010 |
| CONCENTRATION: | 23.94mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSHPPV DYHDFPSLRC ELGDDGVLTV VLDSPGLNSV GPQMHRDLAD IWPVIDRDPA VRAVLVRGEG KAFSSGGSFD LIDETIGDYQ GRIRIMREAR DLVHNMINCD TPVVSAIRGP AVGAGLVVAL LADISVAGRT AKLIDGHTKL GVAAGDHAAI CWPLLVGMAK AKYYLLTCET LLGEEAERIG LVSLCVDDDD VLSTAAGIAG KLAQGAQHAI QWTKRSLNHW YRMMGPTFET SVGLEFLSFS GPDVQEGLAA HREKRAARFT GGSAT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc cagtcagcca cccgccggtc gattaccacg acttccccag cctgcgctgc gaactcggcg acgacggggt gctgacggtg gtgctcgact cgccgggtct gaattcggtg gggccgcaga tgcaccgcga cctggccgac atctggccgg tgatcgaccg cgacccggcg gtgcgcgcgg tgctggtccg cggtgagggc aaggccttct cctccggcgg cagcttcgac ctgatcgacg agaccatcgg cgattaccag ggccggatcc gcatcatgcg ggaggcccgc gacctagtgc acaacatgat caactgcgac accccggtgg tgtcggcgat ccgcgggccg gccgtgggcg cgggcctggt ggtggccctg ctcgccgaca tctcggtggc cggccgcacc gccaagctga tcgacgggca caccaagctg ggcgtggccg ccggcgacca cgccgccatc tgctggccgc tgctggtcgg catggccaag gccaagtact acctgctgac ctgcgagacg ctgctcggcg aggaggctga gcggatcggc ctggtgtcgc tgtgcgtcga cgacgacgac gtgctgtcca ccgccgcggg catcgccggg aaactggcgc agggcgccca gcacgccatc caatggacca agcgcagcct caatcactgg taccgcatga tgggccccac cttcgagacc tcggtggggc tggaattcct cagtttcagc gggcccgacg tgcaggaagg cctggccgcg caccgggaaa aacgcgccgc ccgcttcacc ggcggatctg ccacctaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSHPPV DYHDFPSLRC ELGDDGVLTV VLDSPGLNSV GPQMHRDLAD IWPVIDRDPA VRAVLVRGEG KAFSSGGSFD LIDETIGDYQ GRVRIMREAR DLVHNMINCD TPVVSAIRGP AVGAGLVVAL LADISVAGRT AKLIDGHTKL GVAAGDHAAI CWPLLVGMAK AKYYLLTCET LLGEEAERIG LVSLCVDDDD VLSTAAGIAG KLAQGAQHAI QWTKRSLNHW YRTMGPTFET SVGLEFLSFS GPDVQEGLAA HREKRAARFT GGSAT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCAGTC AGCCACCCGC CGGTCGATTA CCACGACTTC CCCAGCCTGC GCTGCGAACT CGGCGACGAC GGGGTGCTGA CGGTGGTGCT CGACTCGCCG GGTCTGAATT CGGTGGGGCC GCAGATGCAC CGCGACCTGG CCGACATCTG GCCGGTGATC GACCGCGACC CGGCGGTGCG CGCGGTGCTG GTCCGCGGTG AGGGCAAGGC CTTCTCCTCC GGCGGCAGCT TCGACCTGAT CGACGAGACC ATCGGCGACT ACCAGGGCCG GGTCCGCATC ATGCGGGAAG CCCGCGACCT GGTGCACAAC ATGATCAACT GCGACACCCC GGTGGTGTCG GCGATCCGCG GGCCGGCCGT GGGCGCGGGC CTGGTGGTGG CCCTGCTCGC CGACATCTCG GTGGCCGGCC GCACCGCCAA GCTGATCGAC GGGCACACCA AGCTGGGCGT GGCCGCCGGC GACCACGCCG CCATCTGCTG GCCGCTGCTG GTCGGCATGG CCAAGGCCAA GTACTACCTG CTGACCTGCG AGACGCTGCT CGGCGAGGAG GCCGAGCGGA TCGGCCTGGT GTCGCTGTGC GTCGACGACG ACGACGTGCT GTCCACCGCC GCGGGCATCG CCGGGAAACT GGCGCAGGGC GCCCAGCACG CCATCCAATG GACCAAGCGC AGCCTCAACC ACTGGTACCG CACGATGGGC CCCACCTTCG AGACCTCGGT GGGGCTGGAA TTCCTCAGTT TCAGCGGGCC CGACGTGCAG GAAGGCCTGG CCGCGCACCG GGAGAAACGC GCCGCCCGCT TCACCGGCGG ATCTGCCACC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA