SSGCID: Target Page

MythA.00358.d

Enoyl-CoA hydratase (A0QXQ8 homolog)

CENTER ID:	MythA.00358.d
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00358.d.A1.GE28730	Full length( MythA.00358.d )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2874
RefSeq:	WP_003926407.1
UniProt:	G7CH33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDYGRFAPTL LVTKDGPVRI LTMNRPDDLN SFNDDLHSAM RRIWDALIDD VDARAVVLTG AGRAFSSGGY LPNFARNNTD PVARRKDIRE AERLAKAMLE CELPIVAAVN GLAVGLGGSL AVMSDLVVMA EDAFISDPHV SVGLVAGDGG ALTWPFMMSM LKAKEYILLG ERITASEAER LGLANRVTPR ERVVEEAVAL AHRLADQPAQ ALRDTKRAMN LHLLNAANMI LPFALATESE TFASEDVRRS VEKFLSKDAS R

NT Sequence

ATGGACTACG GACGCTTCGC CCCCACACTG CTGGTGACCA AGGACGGCCC GGTGCGGATT CTCACCATGA ACCGCCCGGA CGATCTGAAC TCGTTCAATG ATGATCTGCA CAGTGCGATG CGGCGGATCT GGGATGCGCT CATCGACGAC GTGGACGCGC GAGCGGTGGT CCTGACCGGG GCCGGTCGTG CGTTCAGTTC CGGCGGCTAC CTGCCCAACT TCGCGCGGAA CAACACCGAT CCGGTGGCGC GCCGCAAGGA TATCCGTGAG GCGGAGCGGC TGGCCAAGGC CATGCTCGAA TGTGAATTGC CCATCGTCGC CGCGGTCAAC GGACTCGCGG TCGGACTGGG CGGATCCCTG GCCGTGATGT CCGACCTGGT CGTGATGGCC GAGGACGCGT TCATCAGCGA TCCGCACGTC AGCGTCGGGT TGGTCGCCGG CGACGGGGGA GCGCTCACCT GGCCGTTCAT GATGAGCATG CTCAAGGCCA AGGAGTACAT CCTGCTCGGG GAACGCATCA CGGCGTCCGA GGCGGAGCGG TTGGGCCTCG CCAACCGGGT GACGCCGCGC GAACGGGTGG TCGAGGAGGC GGTGGCCCTG GCGCATCGGC TGGCCGATCA GCCGGCGCAG GCGCTGCGCG ACACCAAGCG CGCGATGAAT CTGCACCTGC TCAACGCCGC GAACATGATC CTGCCGTTCG CGCTCGCGAC GGAGTCGGAG ACGTTCGCCA GTGAGGATGT TCGGCGAAGT GTCGAGAAGT TCCTCAGCAA GGATGCCTCC CGA

Details for MythA.00358.d.A1.GE28730

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDYGRFAPT LLVTKDGPVR ILTMNRPDDL NSFNDDLHSA MRRIWDALID DVDARAVVLT GAGRAFSSGG YLPNFARNNT DPVARRKDIR EAERLAKAML ECELPIVAAV NGLAVGLGGS LAVMSDLVVM AEDAFISDPH VSVGLVAGDG GALTWPFMMS MLKAKEYILL GERITASEAE RLGLANRVTP RERVVEEAVA LAHRLADQPA QALRDTKRAM NLHLLNAANM ILPFALATES ETFASEDVRR SVEKFLSKDA SR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggact acggacgctt cgcccccaca ctgctggtga ccaaggacgg cccggtgcgg attctcacca tgaaccgccc ggacgatctg aactcgttca atgatgatct gcacagtgcg atgcggcgga tctgggatgc gctcatcgac gacgtggacg cgcgagcggt ggtcctgacc ggggccggtc gtgcgttcag ttccggcggc tacctgccca acttcgcgcg gaacaacacc gatccggtgg cgcgccgcaa ggatatccgt gaggcggagc ggctggccaa ggccatgctc gaatgtgaat tgcccatcgt cgccgcggtc aacggactcg cggtcggact gggcggatcc ctggccgtga tgtccgacct ggtcgtgatg gccgaggacg cgttcatcag cgatccgcac gtcagcgtcg ggttggtcgc cggcgacggg ggagcgctca cctggccgtt catgatgagc atgctcaagg ccaaggagta catcctgctc ggggaacgca tcacggcgtc cgaggcggag cggttgggcc tcgccaaccg ggtgacgccg cgcgaacggg tggtcgagga ggcggtggcc ctggcgcatc ggctggccga tcagccggcg caggcgctgc gcgacaccaa gcgcgcgatg aatctgcacc tgctcaacgc cgcgaacatg atcctgccgt tcgcgctcgc gacggagtcg gagacgttcg ccagtgagga tgttcggcga agtgtcgaga agttcctcag caaggatgcc tcccgataat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacgga cgcttcgccc ccacactgct ggtgaccaag gacggcccgg tgcggattct caccatgaac cgcccggacg atctgaactc gttcaatgat gatctgcaca gtgcgatgcg gcggatctgg gatgcgctca tcgacgacgt ggacgcgcga gcggtggtcc tgaccggggc cggtcgtgcg ttcagttccg gcggctacct gcccaacttc gcgcggaaca acaccgatcc ggtggcgcgc cgcaaggata tccgtgaggc ggagcggctg gccaaggcca tgctcgaatg tgaattgccc atcgtcgccg cggtcaacgg actcgcggtc ggactgggcg gatccctggc cgtgatgtcc gacctggtcg tgatggccga ggacgcgttc atcagcgatc cgcacgtcag cgtcgggttg gtcgccggcg acgggggagc gctcacctgg ccgttcatga tgagcatgct caaggccaag gagtacatcc tgctcgggga acgcatcacg gcgtccgagg cggagcggtt gggcctcgcc aaccgggtga cgccgcgcga acgggtggtc gaggaggcgg tggccctggc gcatcggctg gccgatcagc cggcgcaggc gctgcgcgac accaagcgcg cgatgaatct gcacctgctc aacgccgcga acatgatcct gccgttcgcg ctcgcgacgg agtcggagac gttcgccagt gaggatgttc ggcgaagtgt cgagaagttc ctcagcaagg atgcctcccg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa