MymaA.00829.c

Enoyl-CoA hydratase

CENTER ID: MymaA.00829.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00829.c.A1.GE30081 Full length( MymaA.00829.c ) 1 255
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5143
RefSeq: YP_001853049.1
UniProt: B2HG82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYDMPTEIDV RAEGALRIIT LNRPDALNAV NDNLHVGLAG LWRRLSEDRS ARAAVLTGAG RAFSAGGDFA YLAELSQDAD LRAKTIAHGR DIVLGMARCR VPVVAAVNGP AVGLGCSLVA LSDIVYIAPD AYLADPHVQV GLVAADGGPL TWPLHISLML AKEYALTGQR IAAQRAVELG LVNHVAADPV AEAVACAQRI MKLPQQAVES TKRVLNIHLE RAVLASLDYA LSAEHQSFTT EDFRSIVTRL TAPKN
NT Sequence
ATGTATGACA TGCCAACAGA AATCGACGTT CGCGCCGAGG GGGCGCTGCG CATCATCACC CTGAACCGGC CCGATGCGCT CAATGCGGTC AATGACAATC TGCATGTTGG GCTCGCAGGG CTGTGGCGGC GGTTGAGCGA GGATCGCAGC GCGCGCGCCG CGGTGCTCAC GGGCGCCGGG CGAGCCTTCT CGGCCGGCGG CGATTTCGCG TATCTGGCCG AACTCTCCCA AGACGCCGAC CTGCGGGCGA AGACCATCGC GCATGGCCGA GACATCGTGC TGGGCATGGC CCGCTGCCGG GTGCCGGTGG TGGCGGCGGT CAACGGCCCG GCGGTGGGGC TCGGCTGCAG CCTGGTCGCC CTGAGTGACA TCGTCTACAT CGCGCCGGAT GCCTATCTTG CCGACCCACA TGTGCAAGTG GGTCTGGTCG CCGCCGACGG CGGTCCGTTG ACCTGGCCAT TGCACATCAG CCTGATGCTG GCCAAGGAAT ACGCGTTGAC CGGTCAGCGG ATTGCCGCGC AGCGCGCCGT CGAGCTGGGC CTGGTCAACC ATGTGGCCGC CGATCCGGTG GCCGAGGCGG TGGCGTGCGC CCAGCGGATC ATGAAATTGC CTCAGCAGGC GGTCGAAAGC ACCAAACGGG TGCTCAATAT TCACCTCGAG CGGGCCGTGC TCGCCAGTCT GGACTACGCG CTGTCGGCCG AGCACCAGTC GTTCACCACC GAGGACTTCC GCTCAATCGT GACCAGGTTG ACCGCTCCGA AGAAC
Details for MymaA.00829.c.A1.GE30081
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMYDMPTE IDVRAEGALR IITLNRPDAL NAVNDNLHVG LAGLWRRLSE DRSARAAVLT GAGRAFSAGG DFAYLAELSQ DADLRAKTIA HGRDIVLGMA RCRVPVVAAV NGPAVGLGCS LVALSDIVYI APDAYLADPH VQVGLVAADG GPLTWPLHIS LMLAKEYALT GQRIAAQRAV ELGLVNHVAA DPVAEAVACA QRIMKLPQQA VESTKRVLNI HLERAVLASL DYALSAEHQS FTTEDFRSIV TRLTAPK
Validated NT Sequence
ttattacttc ggagcggtca acctggtcac gattgagcgg aagtcctcgg tggtgaacga ctggtgctcg gccgacagcg cgtagtccag actggcgagc acggcccgct cgaggtgaat attgagcacc cgtttggtgc tttcgaccgc ctgctgaggc aatttcatga tccgctgggc gcacgccacc gcctcggcca ccggatcggc ggccacatgg ttgaccaggc ccagctcgac ggcgcgctgc gcggcaatcc gctgaccggt caacgcgtat tccttggcca gcatcaggct gatgtgcaat ggccaggtca acggaccgcc gtcggcggcg accagaccca cttgcacatg tgggtcggca agataggcat ccggcgcgat gtagacgatg tcactcaggg cgaccaggct gcagccgagc cccaccgccg ggccgttgac cgccgccacc accggcaccc ggcagcgggc catgcccagc acgatgtctc ggccatgcgc gatggtcttc gcccgcaggt cggcgtcttg ggagagttcg gccagatacg cgaaatcgcc gccggccgag aaggctcgcc cggcgcccgt gagcaccgcg gcgcgcgcgc tgcgatcctc gctcaaccgc cgccacagcc ctgcgagccc aacatgcaga ttgtcattga ccgcattgag cgcatcgggc cggttcaggg tgatgatgcg cagcgccccc tcggcgcgaa cgtcgatttc tgttggcatg tcatacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYDMPTEID VRAEGALRII TLNRPDALNA VNDNLHVGLA GLWRRLSEDR SARAAVLTGA GRAFSAGGDF AYLAELSQDA DLRAKTIAHG RDIVLGMARC RVPVVAAVNG PAVGLGCSLV ALSDIVYIAP DAYLADPHVQ VGLVAADGGP LTWPLHISLM LAKEYALTGQ RIAAQRAVEL GLVNHVAADP VAEAVACAQR IMKLPQQAVE STKRVLNIHL ERAVLASLDY ALSAEHQSFT TEDFRSIVTR LTAPK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtatgacatg ccaacagaaa tcgacgttcg cgccgagggg gcgctgcgca tcatcaccct gaaccggccc gatgcgctca atgcggtcaa tgacaatctg catgttgggc tcgcagggct gtggcggcgg ttgagcgagg atcgcagcgc gcgcgccgcg gtgctcacgg gcgccgggcg agccttctcg gccggcggcg atttcgcgta tctggccgaa ctctcccaag acgccgacct gcgggcgaag accatcgcgc atggccgaga catcgtgctg ggcatggccc gctgccgggt gccggtggtg gcggcggtca acggcccggc ggtggggctc ggctgcagcc tggtcgccct gagtgacatc gtctacatcg cgccggatgc ctatcttgcc gacccacatg tgcaagtggg tctggtcgcc gccgacggcg gtccgttgac ctggccattg cacatcagcc tgatgctggc caaggaatac gcgttgaccg gtcagcggat tgccgcgcag cgcgccgtcg agctgggcct ggtcaaccat gtggccgccg atccggtggc cgaggcggtg gcgtgcgccc agcggatcat gaaattgcct cagcaggcgg tcgaaagcac caaacgggtg ctcaatattc acctcgagcg ggccgtgctc gccagtctgg actacgcgct gtcggccgag caccagtcgt tcaccaccga ggacttccgc tcaatcgtga ccaggttgac cgctccgaag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa