SSGCID: Target Page

MyulA.00829.c

Enoyl-CoA hydratase EchA15

CENTER ID:	MyulA.00829.c
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00829.c.A1.GE29745	Full length( MyulA.00829.c )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.3853
RefSeq:	YP_906975.1
UniProt:	A0PT35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLTYQDFPS LRFEPGERGV LNLVLDSPGL NSVGPQMHRD LTDVWPVIDR DPDVRVVLVR GEGKAFSSGG SFELIDETIG DYEGRIRIMR EARDLVLSLV NLDKPVVSAI RGPAVGARLV VALLADISVA SATAKIIDGH TKLGVAAGDH AAICWPLLVG MAKAKYYLLT CETLSGEEAE RISLVSTCVD DDEVLPTATR LAEDLAQGAQ NAIRWTKRSL NHWYRMFGPT FETSLGLEFL GFTGPDVQEG LAAHRQKRPA RFTDRTEGP

NT Sequence

ATGCCACTCA CCTACCAAGA TTTCCCCAGC CTTCGCTTCG AGCCGGGTGA GCGCGGCGTG CTGAATCTGG TGCTGGACTC CCCCGGGCTG AATTCGGTCG GACCGCAGAT GCACCGCGAC CTCACTGACG TCTGGCCGGT GATCGACCGT GACCCCGACG TGCGGGTGGT GCTGGTTCGT GGTGAGGGCA AAGCGTTTTC GTCCGGGGGC AGTTTTGAGC TCATCGACGA AACGATCGGC GACTACGAAG GCCGAATCCG CATCATGCGC GAGGCCCGTG ACCTGGTGCT CAGCCTGGTC AACCTCGACA AGCCGGTGGT TTCGGCGATT CGCGGCCCGG CCGTTGGCGC CCGTCTGGTG GTAGCGCTGC TCGCCGATAT CTCGGTGGCA AGCGCAACCG CGAAAATCAT CGACGGCCAC ACCAAGCTCG GGGTGGCGGC CGGTGACCAC GCCGCGATCT GCTGGCCGCT GCTGGTCGGC ATGGCCAAGG CCAAGTACTA CCTACTCACC TGCGAGACAC TGTCCGGCGA AGAAGCTGAA CGCATCAGCC TGGTCTCGAC GTGCGTCGAT GACGATGAGG TGCTGCCCAC CGCAACCCGG CTGGCCGAGG ATCTGGCGCA GGGGGCGCAA AACGCGATCC GCTGGACCAA GCGCAGCCTC AATCACTGGT ACCGGATGTT CGGGCCGACC TTCGAAACGT CGCTTGGCCT GGAGTTTCTC GGGTTCACCG GCCCCGATGT GCAGGAAGGC CTGGCCGCGC ACCGGCAGAA GCGTCCCGCC CGGTTTACCG ACCGCACCGA GGGGCCC

Details for MyulA.00829.c.A1.GE29745

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	31kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPLTYQDFP SLRFEPGERG VLNLVLDSPG LNSVGPQMHR DLTDVWPVID RDPDVRVVLV RGEGKAFSSG GSFELIDETI GDYEGRIRIM REARDLVLSL VNLDKPVVSA IRGPAVGARL VVALLADISV ASATAKIIDG HTKLGVAAGD HAAICWPLLV GMAKAKYYLL TCETLSGEEA ERISLVSTCV DDDEVLPTAT RLAEDLAQGA QNAIRWTKRS LNHWYRMFGP TFETSLXLXF LGFTGPDVQX GLAAHRQKRP ARFTDRTEGP

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccac tcacctacca agatttcccc agccttcgct tcgagccggg tgagcgcggc gtgctgaatc tggtgctgga ctcccccggg ctgaattcgg tcggaccgca gatgcaccgc gacctcactg acgtctggcc ggtgatcgac cgtgaccccg acgtgcgggt ggtgctggtt cgtggtgagg gcaaagcgtt ttcgtccggg ggcagttttg agctcatcga cgaaacgatc ggcgactacg aaggccgaat ccgcatcatg cgcgaggccc gtgacctggt gctcagcctg gtcaacctcg acaagccggt ggtttcggcg attcgcggcc cggccgttgg cgcccgtctg gtggtagcgc tgctcgccga tatctcggtg gcaagcgcaa ccgcgaaaat catcgacggc cacaccaagc tcggggtggc ggccggtgac cacgccgcga tctgctggcc gctgctggtc ggcatggcca aggccaagta ctacctactc acctgcgaga cactgtccgg cgaagaagct gaacgcatca gcctggtctc gacgtgcgtc gatgacgatg aggtgctgcc caccgcaacc cggctggccg aggatctggc gcagggggcg caaaacgcga tccgctggac caagcgcagc ctcaatcact ggtaccggat gttcgggccg accttcgaaa cgtcgcttgn nctnnngttt ctcgggttca ccggccccga tgtgcagnaa ggnctggccg cgcaccggca gaagcgtccc gcccggttta ccgaccgcac cgaggggccc taataaacag cacgaacnag tnctgcagcc aagcttctcg aggatccngc tgctancnaa gcccgaaagg aagctgantt ggctgctgcc nccnnnnnnn nnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccactcacc taccaagatt tccccagcct tcgcttcgag ccgggtgagc gcggcgtgct gaatctggtg ctggactccc ccgggctgaa ttcggtcgga ccgcagatgc accgcgacct cactgacgtc tggccggtga tcgaccgtga ccccgacgtg cgggtggtgc tggttcgtgg tgagggcaaa gcgttttcgt ccgggggcag ttttgagctc atcgacgaaa cgatcggcga ctacgaaggc cgaatccgca tcatgcgcga ggcccgtgac ctggtgctca gcctggtcaa cctcgacaag ccggtggttt cggcgattcg cggcccggcc gttggcgccc gtctggtggt agcgctgctc gccgatatct cggtggcaag cgcaaccgcg aaaatcatcg acggccacac caagctcggg gtggcggccg gtgaccacgc cgcgatctgc tggccgctgc tggtcggcat ggccaaggcc aagtactacc tactcacctg cgagacactg tccggcgaag aagctgaacg catcagcctg gtctcgacgt gcgtcgatga cgatgaggtg ctgcccaccg caacccggct ggccgaggat ctggcgcagg gggcgcaaaa cgcgatccgc tggaccaagc gcagcctcaa tcactggtac cggatgttcg ggccgacctt cgaaacgtcg cttggcctgg agtttctcgg gttcaccggc cccgatgtgc aggaaggcct ggccgcgcac cggcagaagc gtcccgcccg gtttaccgac cgcaccgagg ggcccaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa