MytuD.01530.a

Enoyl-CoA hydratase

CENTER ID: MytuD.01530.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01530.a.A1.GE25970 Full length( MytuD.01530.a ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01530.a.A1.PS00181 Full length( MytuD.01530.a ) 1 263

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3926
RefSeq: NP_218033.1
TubercuList: Rv3516
MTB Network Portal: Rv3516
UniProt: O53561

Orthologues for Rv3516

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 92 100 1 0 0
marinum 91 100 1 1 1
ulcerans 90 100 1 1 0
smegmatis 84 99 1 2 0
abscessus 79 98 0 0 0
abscessus 48 100 1 1 1
smegmatis 35 97 1 1 0
marinum 35 96 1 0 0
marinum 34 97 0 0 0
ulcerans 34 97 1 0 0
ulcerans 34 96 0 0 0
paratuberculosis 34 95 1 1 1
smegmatis 33 96 0 0 0
abscessus 32 97 1 1 1
avium 31 80 1 2 0
thermoresistibile 30 95 1 1 0
abscessus 28 95 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESGPDALVE RRGHTLIVTM NRPAARNALS TEMMRIMVQA WDRVDNDPDI RCCILTGAGG YFCAGMDLKA ATQKPPGDSF KDGSYGPSRI DALLKGRRLT KPLIAAVEGP AIAGGTEILQ GTDIRVAGES AKFGISEAKW SLYPMGGSAV RLVRQIPYTL ACDLLLTGRH ITAAEAKEMG LIGHVVPDGQ ALTKALELAD AISANGPLAV QAILRSIRET ECMPENEAFK IDTQIGIKVF LSDDAKEGPR AFAEKRAPNF QNR
NT Sequence
GTGGAATCCG GACCCGACGC GCTGGTGGAG CGGCGCGGCC ACACCCTGAT CGTGACCATG AACCGGCCGG CCGCCCGCAA CGCGCTGAGC ACCGAAATGA TGCGAATCAT GGTGCAGGCC TGGGATCGCG TCGACAACGA TCCCGACATC CGTTGCTGCA TCCTCACCGG AGCCGGTGGC TACTTTTGCG CCGGCATGGA CCTCAAGGCG GCAACCCAGA AACCGCCGGG CGACTCTTTC AAGGACGGCA GCTACGGCCC GTCGCGCATC GATGCCCTGC TCAAAGGGCG CCGCTTGACC AAACCGCTGA TCGCCGCCGT CGAGGGGCCC GCGATCGCCG GCGGCACCGA GATCCTGCAG GGCACCGACA TCCGGGTCGC CGGTGAAAGT GCGAAGTTCG GCATCTCCGA GGCCAAGTGG AGCCTGTACC CGATGGGCGG CTCGGCCGTG CGGCTGGTCC GGCAGATCCC CTACACTCTG GCCTGCGACC TGCTGCTGAC CGGACGGCAC ATTACCGCCG CCGAGGCCAA GGAAATGGGC CTGATCGGCC ACGTGGTGCC CGACGGCCAG GCGCTGACCA AGGCTCTAGA ACTTGCCGAC GCCATCTCGG CTAACGGACC CCTGGCCGTG CAGGCCATCC TGCGGTCCAT CCGCGAGACC GAGTGCATGC CCGAAAACGA GGCGTTCAAG ATCGACACCC AGATCGGCAT CAAGGTCTTC CTGTCCGACG ACGCCAAGGA AGGCCCGCGC GCGTTCGCCG AGAAGCGCGC ACCCAACTTC CAGAACCGC
Details for MytuD.01530.a.A1.GE25970
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVESGPDAL VERRGHTLIV TMNRPAARNA LSTEMMRIMV QAWDRVDNDP DIRCCILTGA GGYFCAGMDL KAATQKPPGD SFKDGSYGPS RIDALLKGRR LTKPLIAAVE GPAIAGGTEI LQGTDIRVAG ESAKFGISEA KWSLYPMGGS AVRLVRQIPY TLACDLLLTG RHITAAEAKE MGLIGHVVPD GQALTKALEL ADAISANGPL AVQAILRSIR ETECMPENEA FKIDTQIGIK VFLSDDAKEG PRAFAEKRAP NFQ
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggaa tccggacccg acgcgctggt ggagcggcgc ggccacaccc tgatcgtgac catgaaccgg ccggccgccc gcaacgcgct gagcaccgaa atgatgcgaa tcatggtgca ggcctgggat cgcgtcgaca acgatcccga catccgttgc tgcatcctca ccggagccgg tggctacttt tgcgccggca tggacctcaa ggcggcaacc cagaaaccgc cgggcgactc tttcaaggac ggcagctacg gcccgtcgcg catcgatgcc ctgctcaaag ggcgccgctt gaccaaaccg ctgatcgccg ccgtcgaggg gcccgcgatc gccggcggca ccgagatcct gcagggcacc gacatccggg tcgccggtga aagtgcgaag ttcggcatct ccgaggccaa gtggagcctg tacccgatgg gcggctcggc cgtgcggctg gtccggcaga tcccctacac tctggcctgc gacctgctgc tgaccggacg gcacattacc gccgccgagg ccaaggaaat gggcctgatc ggccacgtgg tgcccgacgg ccaggcgctg accaaggctc tagaacttgc cgacgccatc tcggctaacg gacccctggc cgtgcaggcc atcctgcggt ccatccgcga gaccgagtgc atgcccgaaa acgaggcgtt caagatcgac acccagatcg gcatcaaggt cttcctgtcc gacgacgcca aggaaggccc gcgcgcgttc gccgagaagc gcgcacccaa cttccaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVESGPDAL VERRGHTLIV TMNRPAARNA LSTEMMRIMV QAWDRVDNDP DIRCCILTGA GGYFCAGMDL KAATQKPPGD SFKDGSYGPS RIDALLKGRR LTKPLIAAVE GPAIAGGTEI LQGTDIRVAG ESAKFGISEA KWSLYPMGGS AVRLVRQIPY TLACDLLLTG RHITAAEAKE MGLIGHVVPD GQALTKALEL ADAISANGPL AVQAILRSIR ETECMPENEA FKIDTQIGIK VFLSDDAKEG PRAFAEKRAP NFQNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaatcc ggacccgacg cgctggtgga gcggcgcggc cacaccctga tcgtgaccat gaaccggccg gccgcccgca acgcgctgag caccgaaatg atgcgaatca tggtgcaggc ctgggatcgc gtcgacaacg atcccgacat ccgttgctgc atcctcaccg gagccggtgg ctacttttgc gccggcatgg acctcaaggc ggcaacccag aaaccgccgg gcgactcttt caaggacggc agctacggcc cgtcgcgcat cgatgccctg ctcaaagggc gccgcttgac caaaccgctg atcgccgccg tcgaggggcc cgcgatcgcc ggcggcaccg agatcctgca gggcaccgac atccgggtcg ccggtgaaag tgcgaagttc ggcatctccg aggccaagtg gagcctgtac ccgatgggcg gctcggccgt gcggctggtc cggcagatcc cctacactct ggcctgcgac ctgctgctga ccggacggca cattaccgcc gccgaggcca aggaaatggg cctgatcggc cacgtggtgc ccgacggcca ggcgctgacc aaggctctag aacttgccga cgccatctcg gctaacggac ccctggccgt gcaggccatc ctgcggtcca tccgcgagac cgagtgcatg cccgaaaacg aggcgttcaa gatcgacacc cagatcggca tcaaggtctt cctgtccgac gacgccaagg aaggcccgcg cgcgttcgcc gagaagcgcg cacccaactt ccagaaccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MytuD.01530.a.A1.PS00181
PURIFICATION DATe: 2/25/2009
CONCENTRATION: 51.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVESGPDAL VERRGHTLIV TMNRPAARNA LSTEMMRIMV QAWDRVDNDP DIRCCILTGA GGYFCAGMDL KAATQKPPGD SFKDGSYGPS RIDALLKGRR LTKPLIAAVE GPAIAGGTEI LQGTDIRVAG ESAKFGISEA KWSLYPMGGS AVRLVRQIPY TLACDLLLTG RHITAAEAKE MGLIGHVVPD GQALTKALEL ADAISANGPL AVQAILRSIR ETECMPENEA FKIDTQIGIK VFLSDDAKEG PRAFAEKRAP NFQ
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggaa tccggacccg acgcgctggt ggagcggcgc ggccacaccc tgatcgtgac catgaaccgg ccggccgccc gcaacgcgct gagcaccgaa atgatgcgaa tcatggtgca ggcctgggat cgcgtcgaca acgatcccga catccgttgc tgcatcctca ccggagccgg tggctacttt tgcgccggca tggacctcaa ggcggcaacc cagaaaccgc cgggcgactc tttcaaggac ggcagctacg gcccgtcgcg catcgatgcc ctgctcaaag ggcgccgctt gaccaaaccg ctgatcgccg ccgtcgaggg gcccgcgatc gccggcggca ccgagatcct gcagggcacc gacatccggg tcgccggtga aagtgcgaag ttcggcatct ccgaggccaa gtggagcctg tacccgatgg gcggctcggc cgtgcggctg gtccggcaga tcccctacac tctggcctgc gacctgctgc tgaccggacg gcacattacc gccgccgagg ccaaggaaat gggcctgatc ggccacgtgg tgcccgacgg ccaggcgctg accaaggctc tagaacttgc cgacgccatc tcggctaacg gacccctggc cgtgcaggcc atcctgcggt ccatccgcga gaccgagtgc atgcccgaaa acgaggcgtt caagatcgac acccagatcg gcatcaaggt cttcctgtcc gacgacgcca aggaaggccc gcgcgcgttc gccgagaagc gcgcacccaa cttccaga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVESGPDAL VERRGHTLIV TMNRPAARNA LSTEMMRIMV QAWDRVDNDP DIRCCILTGA GGYFCAGMDL KAATQKPPGD SFKDGSYGPS RIDALLKGRR LTKPLIAAVE GPAIAGGTEI LQGTDIRVAG ESAKFGISEA KWSLYPMGGS AVRLVRQIPY TLACDLLLTG RHITAAEAKE MGLIGHVVPD GQALTKALEL ADAISANGPL AVQAILRSIR ETECMPENEA FKIDTQIGIK VFLSDDAKEG PRAFAEKRAP NFQNR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGAATCC GGACCCGACG CGCTGGTGGA GCGGCGCGGC CACACCCTGA TCGTGACCAT GAACCGGCCG GCCGCCCGCA ACGCGCTGAG CACCGAAATG ATGCGAATCA TGGTGCAGGC CTGGGATCGC GTCGACAACG ATCCCGACAT CCGTTGCTGC ATCCTCACCG GAGCCGGTGG CTACTTTTGC GCCGGCATGG ACCTCAAGGC GGCAACCCAG AAACCGCCGG GCGACTCTTT CAAGGACGGC AGCTACGGCC CGTCGCGCAT CGATGCCCTG CTCAAAGGGC GCCGCTTGAC CAAACCGCTG ATCGCCGCCG TCGAGGGGCC CGCGATCGCC GGCGGCACCG AGATCCTGCA GGGCACCGAC ATCCGGGTCG CCGGTGAAAG TGCGAAGTTC GGCATCTCCG AGGCCAAGTG GAGCCTGTAC CCGATGGGCG GCTCGGCCGT GCGGCTGGTC CGGCAGATCC CCTACACTCT GGCCTGCGAC CTGCTGCTGA CCGGACGGCA CATTACCGCC GCCGAGGCCA AGGAAATGGG CCTGATCGGC CACGTGGTGC CCGACGGCCA GGCGCTGACC AAGGCTCTAG AACTTGCCGA CGCCATCTCG GCTAACGGAC CCCTGGCCGT GCAGGCCATC CTGCGGTCCA TCCGCGAGAC CGAGTGCATG CCCGAAAACG AGGCGTTCAA GATCGACACC CAGATCGGCA TCAAGGTCTT CCTGTCCGAC GACGCCAAGG AAGGCCCGCG CGCGTTCGCC GAGAAGCGCG CACCCAACTT CCAGAACCGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA