MythA.01566.a

Enoyl-CoA hydratase/isomerase family protein (A0QUI2 homolog)

CENTER ID:	MythA.01566.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01566.a.A1.GE28791	Full length( MythA.01566.a )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01566.a.A1.PW28693	Full length( MythA.01566.a )		1	303

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.3746
RefSeq:	WP_003927271.1
UniProt:	G7CLB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKRAWRRSG TAAPRSSRAD DPLTESAEHM SDTSYDTIKY EVDGHTATIT LNRPDALNAL SPHMITELRT AYDEAENDDN VWLLVVTGTG RAFCSGADVK EIPEDGKVIN ERPYLSTYEQ WEAPQEGXXX XXXXXXXXXX XXXXXXXXXC CGAGLDWVTT GDIVIASERA TFFDPHVSIG LVSGREVVRL ARVLPRSVAL RMAIMGRHER MSAQRAYELG MVSEVVEHDR LLERAHEIAD IVTRNAPLAV RGTRLAIVKG LDLPLHEAEI LAEAFRERNL HTEDSLEGPR AFTEKREPRW QCR

NT Sequence

ATTTCAAAGA GGGCCTGGCG GCGTTCCGGG ACCGCCGCGC CCCGGAGTTC CAGGGCCGAT GATCCGCTGA CAGAAAGCGC AGAGCACATG TCCGACACGT CGTACGACAC CATCAAATAC GAGGTCGACG GGCACACCGC GACCATCACG TTGAATCGGC CCGACGCCCT CAACGCGCTG AGCCCGCACA TGATCACCGA ACTGCGAACG GCCTATGACG AGGCCGAGAA CGACGACAAC GTGTGGCTGC TGGTGGTCAC CGGCACCGGT CGGGCGTTCT GCTCCGGCGC CGACGTCAAG GAGATCCCCG AGGACGGCAA GGTCATCAAC GAACGGCCCT ACCTGTCGAC CTACGAGCAG TGGGAGGCTC CGCAGGAGGG CANNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTGT TGTGGCGCCG GCCTGGACTG GGTGACCACC GGTGACATCG TGATCGCCTC CGAGCGGGCG ACGTTCTTCG ATCCGCATGT GAGCATCGGG CTGGTGTCCG GCCGGGAAGT GGTCCGGTTG GCCCGGGTGC TGCCCCGGTC GGTGGCGCTG CGGATGGCCA TCATGGGCAG ACACGAACGG ATGAGCGCCC AGCGCGCCTA CGAACTCGGG ATGGTCAGCG AGGTCGTCGA GCACGACAGG CTGCTGGAAC GCGCCCACGA GATCGCCGAC ATCGTCACCC GCAACGCCCC GCTGGCGGTG CGCGGCACCC GGTTGGCCAT CGTCAAGGGC CTGGACCTGC CGCTGCACGA GGCGGAGATC CTCGCCGAGG CGTTCCGCGA ACGCAACCTG CACACCGAGG ACTCGCTGGA GGGTCCGCGG GCGTTCACGG AGAAGCGCGA ACCCCGGTGG CAGTGTCGA

Details for MythA.01566.a.A1.GE28791

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	94
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMISKRAW RRSGTAAPRS SRADDPLTES AEHMSDTSYD TIKYEVDGHT ATITLNRPDA LNALSPHMIT ELRTAYDEAE NDDNVWLLVV TGTGRAFCSG ADVKEIPEDG KVINERPYLS TYEQWEAPQE GTPPFRTMAK PVLAAINGIC CGAGLDWVTT GDIVIASERA TFFDPHVSIG LVSGREVVRL ARVLPRSVAL RMAIMGRHER MSAQRAYELG MVSEVVEHDR LLERAHEIAD IVTRNAPLAV RGTRLAIVKG LDLPLHEAEI LAEAFRERNL HTEDSLEGPR AFTEKREPRW QCR

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatttc aaagagggcc tggcggcgtt ccgggaccgc cgcgccccgg agttccaggg ccgatgatcc gctgacagaa agcgcagagc acatgtccga cacgtcgtac gacaccatca aatacgaggt cgacgggcac accgcgacca tcacgttgaa tcggcccgac gccctcaacg cgctgagccc gcacatgatc accgaactgc gaacggccta tgacgaggcc gagaacgacg acaacgtgtg gctgctggtg gtcaccggca ccggtcgggc gttctgctcc ggcgccgacg tcaaggagat ccccgaggac ggcaaggtca tcaacgaacg gccctacctg tcgacctacg agcagtggga ggctccgcag gagggcaccc cgccgttccg caccatggcc aaaccggtgc tggcggcgat caacgggatc tgttgtggcg ccggcctgga ctgggtgacc accggtgaca tcgtgatcgc ctccgagcgg gcgacgttct tcgatccgca tgtgagcatc gggctggtgt ccggccggga agtggtccgg ttggcccggg tgctgccccg gtcggtggcg ctgcggatgg ccatcatggg cagacacgaa cggatgagcg cccagcgcgc ctacgaactc gggatggtca gcgaggtcgt cgagcacgac aggctgctgg aacgcgccca cgagatcgcc gacatcgtca cccgcaacgc cccgctggcg gtgcgcggca cccggttggc catcgtcaag ggcctggacc tgccgctgca cgaggcggag atcctcgccg aggcgttccg cgaacgcaac ctgcacaccg aggactcgct ggagggtccg cgggcgttca cggagaagcg cgaaccccgg tggcagtgtc gataataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMISKRAWRR SGTAAPRSSR ADDPLTESAE HMSDTSYDTI KYEVDGHTAT ITLNRPDALN ALSPHMITEL RTAYDEAEND DNVWLLVVTG TGRAFCSGAD VKEIPEDGKV INERPYLSTY EQWEAPQEGX XXXXXXXXXX XXXXXXXXXX XCCGAGLDWV TTGDIVIASE RATFFDPHVS IGLVSGREVV RLARVLPRSV ALRMAIMGRH ERMSAQRAYE LGMVSEVVEH DRLLERAHEI ADIVTRNAPL AVRGTRLAIV KGLDLPLHEA EILAEAFRER NLHTEDSLEG PRAFTEKREP RWQCR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatttcaaag agggcctggc ggcgttccgg gaccgccgcg ccccggagtt ccagggccga tgatccgctg acagaaagcg cagagcacat gtccgacacg tcgtacgaca ccatcaaata cgaggtcgac gggcacaccg cgaccatcac gttgaatcgg cccgacgccc tcaacgcgct gagcccgcac atgatcaccg aactgcgaac ggcctatgac gaggccgaga acgacgacaa cgtgtggctg ctggtggtca ccggcaccgg tcgggcgttc tgctccggcg ccgacgtcaa ggagatcccc gaggacggca aggtcatcaa cgaacggccc tacctgtcga cctacgagca gtgggaggct ccgcaggagg gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ttgtggcgcc ggcctggact gggtgaccac cggtgacatc gtgatcgcct ccgagcgggc gacgttcttc gatccgcatg tgagcatcgg gctggtgtcc ggccgggaag tggtccggtt ggcccgggtg ctgccccggt cggtggcgct gcggatggcc atcatgggca gacacgaacg gatgagcgcc cagcgcgcct acgaactcgg gatggtcagc gaggtcgtcg agcacgacag gctgctggaa cgcgcccacg agatcgccga catcgtcacc cgcaacgccc cgctggcggt gcgcggcacc cggttggcca tcgtcaaggg cctggacctg ccgctgcacg aggcggagat cctcgccgag gcgttccgcg aacgcaacct gcacaccgag gactcgctgg agggtccgcg ggcgttcacg gagaagcgcg aaccccggtg gcagtgtcga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MythA.01566.a.A1.PW28693

PURIFICATION DATe:	7/6/2010
CONCENTRATION:	13.96mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTCAAAG AGGGCCTGGC GGCGTTCCGG GACCGCCGCG CCCCGGAGTT CCAGGGCCGA TGATCCGCTG ACAGAAAGCG CAGAGCACAT GTCCGACACG TCGTACGACA CCATCAAATA CGAGGTCGAC GGGCACACCG CGACCATCAC GTTGAATCGG CCCGACGCCC TCAACGCGCT GAGCCCGCAC ATGATCACCG AACTGCGAAC GGCCTATGAC GAGGCCGAGA ACGACGACAA CGTGTGGCTG CTGGTGGTCA CCGGCACCGG TCGGGCGTTC TGCTCCGGCG CCGACGTCAA GGAGATCCCC GAGGACGGCA AGGTCATCAA CGAACGGCCC TACCTGTCGA CCTACGAGCA GTGGGAGGCT CCGCAGGAGG GCANNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG TTGTGGCGCC GGCCTGGACT GGGTGACCAC CGGTGACATC GTGATCGCCT CCGAGCGGGC GACGTTCTTC GATCCGCATG TGAGCATCGG GCTGGTGTCC GGCCGGGAAG TGGTCCGGTT GGCCCGGGTG CTGCCCCGGT CGGTGGCGCT GCGGATGGCC ATCATGGGCA GACACGAACG GATGAGCGCC CAGCGCGCCT ACGAACTCGG GATGGTCAGC GAGGTCGTCG AGCACGACAG GCTGCTGGAA CGCGCCCACG AGATCGCCGA CATCGTCACC CGCAACGCCC CGCTGGCGGT GCGCGGCACC CGGTTGGCCA TCGTCAAGGG CCTGGACCTG CCGCTGCACG AGGCGGAGAT CCTCGCCGAG GCGTTCCGCG AACGCAACCT GCACACCGAG GACTCGCTGG AGGGTCCGCG GGCGTTCACG GAGAAGCGCG AACCCCGGTG GCAGTGTCGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA