SSGCID: Target Page

MymaA.01530.b

Enoyl-CoA hydratase EchA11_1

CENTER ID:	MymaA.01530.b
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01530.b.A1.GE30185	Full length( MymaA.01530.b )		1	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.4310
RefSeq:	YP_001852285.1
UniProt:	B2HQD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPGDAFDTIG YELDGHTATI TLNRPHALNA LSPHMITELR AAYAEAEGND RVWLTIVTGT GRAFCTGADV KAIPGDGKVI YERPYLATYE QWEAPQEGTP PFRSMAKPVL TAVNGICCGA GLDWVTTSDI VIASEAATFF DPHVSIGLVA GRELVRLARV LPRSVALRMA LVGKHERMDA RRAFDLGLIS EVVEHDRLLA RAHEIADMVN RNAPLAVRGT RLAILKGIDL PLPEAEMLAE AFRERVLRTR DALEGPRAFA EKRDPDWQCQ

NT Sequence

ATGCCTGGCG ACGCGTTCGA CACCATCGGC TACGAGCTCG ACGGACACAC CGCCACCATC ACGCTCAACC GACCTCACGC CCTCAACGCG CTGAGTCCGC ACATGATCAC CGAGTTGCGA GCCGCCTACG CCGAAGCCGA AGGTAACGAC CGGGTGTGGC TGACCATCGT CACCGGCACG GGTCGCGCCT TCTGCACAGG GGCGGACGTG AAGGCCATTC CAGGCGACGG CAAGGTGATC TACGAGCGGC CCTACCTGGC AACCTACGAA CAGTGGGAGG CGCCCCAAGA AGGCACGCCG CCATTTCGCA GCATGGCCAA ACCCGTACTG ACCGCAGTAA ACGGCATCTG CTGCGGCGCC GGACTGGACT GGGTCACTAC ATCGGACATC GTCATCGCGT CGGAGGCGGC AACGTTCTTC GACCCACACG TCAGCATCGG CCTGGTGGCC GGGCGTGAGC TGGTAAGGCT GGCCCGAGTG CTACCGCGAT CGGTCGCGCT GAGGATGGCG CTGGTCGGCA AGCACGAGCG GATGGATGCG AGGCGCGCCT TCGACCTCGG CTTGATCAGC GAGGTGGTCG AGCACGACCG GCTGCTCGCG CGGGCACACG AGATCGCCGA CATGGTCAAC CGCAATGCCC CGCTGGCCGT CCGGGGTACC CGCCTGGCCA TTCTCAAAGG GATCGACCTG CCGCTACCCG AAGCCGAGAT GCTCGCCGAG GCATTCCGCG AGCGGGTACT GCGGACCCGG GATGCGCTCG AAGGCCCCAG AGCCTTCGCG GAGAAGCGAG ACCCGGATTG GCAATGCCAG

Details for MymaA.01530.b.A1.GE30185

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	31kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPGDAFDTI GYELDGHTAT ITLNRPHALN ALSPHMITEL RAAYAEAEGN DRVWLTIVTG TGRAFCTGAD VKAIPGDGKV IYERPYLATY EQWEAPQEGT PPFRSMAKPV LTAVNGICCG AGLDWVTTSD IVIASEAATF FDPHVSIGLV AGRELVRLAR VLPRSVALRM ALVGKHERMD ARRAFDLGLI SEVVEHDRLL ARAHEIADMV NRNAPLAVRG TRLAILKGID LPLPEAEMLA EAFRERVLRT RDALEGPRAF AEKRDPDWQC Q

Validated NT Sequence

ttattactgg cattgccaat ccgggtctcg cttctccgcg aaggctctgg ggccttcgag cgcatcccgg gtccgcagta cccgctcgcg gaatgcctcg gcgagcatct cggcttcggg tagcggcagg tcgatccctt tgagaatggc caggcgggta ccccggacgg ccagcggggc attgcggttg accatgtcgg cgatctcgtg tgcccgcgcg agcagccggt cgtgctcgac cacctcgctg atcaagccga ggtcgaaggc gcgcctcgca tccatccgct cgtgcttgcc gaccagcgcc atcctcagcg cgaccgatcg cggtagcact cgggccagcc ttaccagctc acgcccggcc accaggccga tgctgacgtg tgggtcgaag aacgttgccg cctccgacgc gatgacgatg tccgatgtag tgacccagtc cagtccggcg ccgcagcaga tgccgtttac tgcggtcagt acgggtttgg ccatgctgcg aaatggcggc gtgccttctt ggggcgcctc ccactgttcg taggttgcca ggtagggccg ctcgtagatc accttgccgt cgcctggaat ggccttcacg tccgcccctg tgcagaaggc gcgacccgtg ccggtgacga tggtcagcca cacccggtcg ttaccttcgg cttcggcgta ggcggctcgc aactcggtga tcatgtgcgg actcagcgcg ttgagggcgt gaggtcggtt gagcgtgatg gtggcggtgt gtccgtcgag ctcgtagccg atggtgtcga acgcgtcgcc aggcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctggcgac gcgttcgaca ccatcggcta cgagctcgac ggacacaccg ccaccatcac gctcaaccga cctcacgccc tcaacgcgct gagtccgcac atgatcaccg agttgcgagc cgcctacgcc gaagccgaag gtaacgaccg ggtgtggctg accatcgtca ccggcacggg tcgcgccttc tgcacagggg cggacgtgaa ggccattcca ggcgacggca aggtgatcta cgagcggccc tacctggcaa cctacgaaca gtgggaggcg ccccaagaag gcacgccgcc atttcgcagc atggccaaac ccgtactgac cgcagtaaac ggcatctgct gcggcgccgg actggactgg gtcactacat cggacatcgt catcgcgtcg gaggcggcaa cgttcttcga cccacacgtc agcatcggcc tggtggccgg gcgtgagctg gtaaggctgg cccgagtgct accgcgatcg gtcgcgctga ggatggcgct ggtcggcaag cacgagcgga tggatgcgag gcgcgccttc gacctcggct tgatcagcga ggtggtcgag cacgaccggc tgctcgcgcg ggcacacgag atcgccgaca tggtcaaccg caatgccccg ctggccgtcc ggggtacccg cctggccatt ctcaaaggga tcgacctgcc gctacccgaa gccgagatgc tcgccgaggc attccgcgag cgggtactgc ggacccggga tgcgctcgaa ggccccagag ccttcgcgga gaagcgagac ccggattggc aatgccagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa