MymaA.01530.e
Enoyl-CoA hydratase EchA19
| CENTER ID: | MymaA.01530.e |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01530.e.A1.GE30188 | Full length( MymaA.01530.e ) | 1 | 274 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01530.e.A1.PS00838 | Full length( MymaA.01530.e ) | 1 | 274 |
Structures
4F47
DEPOSITED: 5/10/2012
DETERMINATION: XRay
CLONE: MymaA.01530.e.A1.GE30188
PROTEIN: MymaA.01530.e.A1.PS00838
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216594.6.peg.5361 |
| RefSeq: | YP_001853261.1 |
| UniProt: | B2HI06 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPNTSETPA NGESGPDALV EQRGHTLIVT MNRPSRRNAL SGEMMQIMVE AWDRVDNDPD IRCCILTGAG GYFCAGMDLK AATKKPPGDS FKDGSYDPSR IDALLKGRRL KKPLIAAVEG PAIAGGTEIL QGTDIRVAAE SAKFGISEAK WSLYPMGGSA VRLVRQIPYT VACDLLLTGR HITAAEAKEM GLVGHVVPDG QALTKALEIA EIIAANGPLA VQAILRTIRE TEGMHENEAF KIDTRIGIEV FLSDDAKEGP QAFAQKRKPN FQNR
NT Sequence
GTGGCCCCGA ATACTTCCGA GACGCCAGCA AACGGGGAGT CCGGACCAGA CGCGCTGGTG GAGCAGCGCG GGCACACCCT GATCGTGACG ATGAATCGCC CGTCCCGGCG TAACGCGCTT AGTGGCGAAA TGATGCAAAT CATGGTCGAG GCCTGGGATC GCGTCGACAA CGATCCCGAC ATCCGCTGCT GCATCCTGAC CGGCGCGGGC GGCTACTTCT GCGCTGGGAT GGACCTCAAG GCGGCGACCA AGAAGCCGCC GGGCGACTCA TTCAAGGACG GCAGCTACGA CCCGTCGCGC ATCGACGCGC TGCTGAAGGG CCGCAGGCTG AAAAAGCCGC TGATCGCCGC CGTCGAGGGC CCCGCGATCG CCGGCGGAAC CGAGATTCTG CAAGGTACCG ACATTCGCGT CGCCGCCGAG AGCGCCAAGT TCGGCATCTC GGAGGCCAAG TGGAGCCTGT ACCCGATGGG AGGCTCGGCG GTGCGACTGG TCCGCCAGAT CCCCTACACG GTGGCCTGCG ATCTCCTGCT GACCGGACGG CACATCACTG CCGCGGAGGC CAAGGAGATG GGGCTGGTGG GCCACGTCGT GCCCGACGGC CAGGCGCTGA CCAAGGCGCT GGAAATTGCG GAGATCATCG CGGCCAACGG GCCTCTTGCG GTCCAGGCGA TCCTGCGCAC CATCCGCGAA ACCGAGGGCA TGCACGAGAA CGAGGCGTTC AAGATCGACA CTCGGATCGG CATCGAGGTG TTCTTGTCCG ACGACGCCAA AGAAGGTCCG CAGGCGTTCG CGCAGAAGCG CAAACCCAAC TTCCAGAACC GC
Details for MymaA.01530.e.A1.GE30188
| HARVESTED ON: | 8/15/2010 |
| SEQUENCED ON: | 8/25/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPNTSET PANGESGPDA LVEQRGHTLI VTMNRPSRRN ALSGEMMQIM VEAWDRVDND PDIRCCILTG AGGYFCAGMD LKAATKKPPG DSFKDGSYDP SRIDALLKGR RLKKPLIAAV EGPAIAGGTE ILQGTDIRVA AESAKFGISE AKWSLYPMGG SAVRLVRQIP YTVACDLLLT GRHITAAEAK EMGLVGHVVP DGQALTKALE IAEIIAANGP LAVQAILRTI RETEGMHENE AFKIDTRIGI EVFLSDDAKE GPQAFAQKRK PNFQ
Validated NT Sequence
tctggaagtt gggtttgcgc ttctgcgcga acgcctgcgg accttctttg gcgtcgtcgg acaagaacac ctcgatgccg atccgagtgt cgatcttgaa cgcctcgttc tcgtgcatgc cctcggtttc gcggatggtg cgcaggatcg cctggaccgc aagaggcccg ttggccgcga tgatctccgc aatttccagc gccttggtca gcgcctggcc gtcgggcacg acgtggccca ccagccccat ctccttggcc tccgcggcag tgatgtgccg tccggtcagc aggagatcgc aggccaccgt gtaggggatc tggcggacca gtcgcaccgc cgagcctccc atcgggtaca ggctccactt ggcctccgag atgccgaact tggcgctctc ggcggcgacg cgaatgtcgg taccttgcag aatctcggtt ccgccggcga tcgcggggcc ctcgacggcg gcgatcagcg gctttttcag cctgcggccc ttcagcagcg cgtcgatgcg cgacgggtcg tagctgccgt ccttgaatga gtcgcccggc ggcttcttgg tcgccgcctt gaggtccatc ccagcgcaga agtagccgcc cgcgccggtc aggatgcagc agcggatgtc gggatcgttg tcgacgcgat cccaggcctc gaccatgatt tgcatcattt cgccactaag cgcgttacgc cgggacgggc gattcatcgt cacgatcagg gtgtgcccgc gctgctccac cagcgcgtct ggtccggact ccccgtttgc tggcgtctcg gaagtattcg gggccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPNTSET PANGESGPDA LVEQRGHTLI VTMNRPSRRN ALSGEMMQIM VEAWDRVDND PDIRCCILTG AGGYFCAGMD LKAATKKPPG DSFKDGSYDP SRIDALLKGR RLKKPLIAAV EGPAIAGGTE ILQGTDIRVA AESAKFGISE AKWSLYPMGG SAVRLVRQIP YTVACDLLLT GRHITAAEAK EMGLVGHVVP DGQALTKALE IAEIIAANGP LAVQAILRTI RETEGMHENE AFKIDTRIGI EVFLSDDAKE GPQAFAQKRK PNFQNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccccg aatacttccg agacgccagc aaacggggag tccggaccag acgcgctggt ggagcagcgc gggcacaccc tgatcgtgac gatgaatcgc ccgtcccggc gtaacgcgct tagtggcgaa atgatgcaaa tcatggtcga ggcctgggat cgcgtcgaca acgatcccga catccgctgc tgcatcctga ccggcgcggg cggctacttc tgcgctggga tggacctcaa ggcggcgacc aagaagccgc cgggcgactc attcaaggac ggcagctacg acccgtcgcg catcgacgcg ctgctgaagg gccgcaggct gaaaaagccg ctgatcgccg ccgtcgaggg ccccgcgatc gccggcggaa ccgagattct gcaaggtacc gacattcgcg tcgccgccga gagcgccaag ttcggcatct cggaggccaa gtggagcctg tacccgatgg gaggctcggc ggtgcgactg gtccgccaga tcccctacac ggtggcctgc gatctcctgc tgaccggacg gcacatcact gccgcggagg ccaaggagat ggggctggtg ggccacgtcg tgcccgacgg ccaggcgctg accaaggcgc tggaaattgc ggagatcatc gcggccaacg ggcctcttgc ggtccaggcg atcctgcgca ccatccgcga aaccgagggc atgcacgaga acgaggcgtt caagatcgac actcggatcg gcatcgaggt gttcttgtcc gacgacgcca aagaaggtcc gcaggcgttc gcgcagaagc gcaaacccaa cttccagaac cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.01530.e.A1.PS00838
| PURIFICATION DATe: | 11/3/2010 |
| CONCENTRATION: | 123.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPNTSET PANGESGPDA LVEQRGHTLI VTMNRPSRRN ALSGEMMQIM VEAWDRVDND PDIRCCILTG AGGYFCAGMD LKAATKKPPG DSFKDGSYDP SRIDALLKGR RLKKPLIAAV EGPAIAGGTE ILQGTDIRVA AESAKFGISE AKWSLYPMGG SAVRLVRQIP YTVACDLLLT GRHITAAEAK EMGLVGHVVP DGQALTKALE IAEIIAANGP LAVQAILRTI RETEGMHENE AFKIDTRIGI EVFLSDDAKE GPQAFAQKRK PNFQ
Validated NT Sequence
tctggaagtt gggtttgcgc ttctgcgcga acgcctgcgg accttctttg gcgtcgtcgg acaagaacac ctcgatgccg atccgagtgt cgatcttgaa cgcctcgttc tcgtgcatgc cctcggtttc gcggatggtg cgcaggatcg cctggaccgc aagaggcccg ttggccgcga tgatctccgc aatttccagc gccttggtca gcgcctggcc gtcgggcacg acgtggccca ccagccccat ctccttggcc tccgcggcag tgatgtgccg tccggtcagc aggagatcgc aggccaccgt gtaggggatc tggcggacca gtcgcaccgc cgagcctccc atcgggtaca ggctccactt ggcctccgag atgccgaact tggcgctctc ggcggcgacg cgaatgtcgg taccttgcag aatctcggtt ccgccggcga tcgcggggcc ctcgacggcg gcgatcagcg gctttttcag cctgcggccc ttcagcagcg cgtcgatgcg cgacgggtcg tagctgccgt ccttgaatga gtcgcccggc ggcttcttgg tcgccgcctt gaggtccatc ccagcgcaga agtagccgcc cgcgccggtc aggatgcagc agcggatgtc gggatcgttg tcgacgcgat cccaggcctc gaccatgatt tgcatcattt cgccactaag cgcgttacgc cgggacgggc gattcatcgt cacgatcagg gtgtgcccgc gctgctccac cagcgcgtct ggtccggact ccccgtttgc tggcgtctcg gaagtattcg gggccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPNTSET PANGESGPDA LVEQRGHTLI VTMNRPSRRN ALSGEMMQIM VEAWDRVDND PDIRCCILTG AGGYFCAGMD LKAATKKPPG DSFKDGSYDP SRIDALLKGR RLKKPLIAAV EGPAIAGGTE ILQGTDIRVA AESAKFGISE AKWSLYPMGG SAVRLVRQIP YTVACDLLLT GRHITAAEAK EMGLVGHVVP DGQALTKALE IAEIIAANGP LAVQAILRTI RETEGMHENE AFKIDTRIGI EVFLSDDAKE GPQAFAQKRK PNFQNR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCCCG AATACTTCCG AGACGCCAGC AAACGGGGAG TCCGGACCAG ACGCGCTGGT GGAGCAGCGC GGGCACACCC TGATCGTGAC GATGAATCGC CCGTCCCGGC GTAACGCGCT TAGTGGCGAA ATGATGCAAA TCATGGTCGA GGCCTGGGAT CGCGTCGACA ACGATCCCGA CATCCGCTGC TGCATCCTGA CCGGCGCGGG CGGCTACTTC TGCGCTGGGA TGGACCTCAA GGCGGCGACC AAGAAGCCGC CGGGCGACTC ATTCAAGGAC GGCAGCTACG ACCCGTCGCG CATCGACGCG CTGCTGAAGG GCCGCAGGCT GAAAAAGCCG CTGATCGCCG CCGTCGAGGG CCCCGCGATC GCCGGCGGAA CCGAGATTCT GCAAGGTACC GACATTCGCG TCGCCGCCGA GAGCGCCAAG TTCGGCATCT CGGAGGCCAA GTGGAGCCTG TACCCGATGG GAGGCTCGGC GGTGCGACTG GTCCGCCAGA TCCCCTACAC GGTGGCCTGC GATCTCCTGC TGACCGGACG GCACATCACT GCCGCGGAGG CCAAGGAGAT GGGGCTGGTG GGCCACGTCG TGCCCGACGG CCAGGCGCTG ACCAAGGCGC TGGAAATTGC GGAGATCATC GCGGCCAACG GGCCTCTTGC GGTCCAGGCG ATCCTGCGCA CCATCCGCGA AACCGAGGGC ATGCACGAGA ACGAGGCGTT CAAGATCGAC ACTCGGATCG GCATCGAGGT GTTCTTGTCC GACGACGCCA AAGAAGGTCC GCAGGCGTTC GCGCAGAAGC GCAAACCCAA CTTCCAGAAC CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA