MysmA.01530.c

Enoyl-CoA hydratase

CENTER ID: MysmA.01530.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01530.c.A1.GE28942 Full length( MysmA.01530.c ) 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01530.c.A1.PS00771 Full length( MysmA.01530.c ) 1 266
MysmA.01530.c.A1.PW30072 Full length( MysmA.01530.c ) 1 266

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5755
RefSeq: YP_890141.1
UniProt: A0R4Q3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETGKGPDA LVEQRGHTLI VTLNRPEARN ALSGEMLSIM VEAWDRVDND PEIRTCILTG AGGYFCSGMD LKGADKKPPG DSFKDGSYDP SKIPGLLKGR RLTKPLIAAV EGPAIAGGTE ILQGTDIRVA GESAKFGISE AKWSLYPMGG SAVRLVRQIP YTIACDMLLT GRHITAAEAK EYGLIGYVVP DGTALDKALE IAETINNNGP LAVQAILKTI RETEGMHEDE AFGPDTRNGI PVFLSEDAKE GPRAFKEKRA PNFQMK
NT Sequence
GTGAGTGAGA CCGGCAAGGG GCCTGACGCC CTCGTTGAGC AGCGCGGACA CACCCTCATC GTCACGTTGA ACCGTCCTGA GGCGCGCAAC GCTCTCTCGG GCGAGATGCT CTCGATCATG GTCGAAGCAT GGGACCGGGT CGACAACGAT CCGGAGATCC GCACCTGCAT CCTGACCGGC GCCGGCGGTT ACTTCTGCTC GGGCATGGAC CTCAAGGGCG CGGACAAGAA GCCGCCGGGT GACTCGTTCA AGGACGGCAG CTACGACCCG TCGAAGATTC CAGGCCTGCT CAAGGGCCGC CGTCTGACCA AGCCGCTGAT CGCCGCGGTC GAGGGTCCCG CCATCGCGGG CGGCACCGAG ATCCTGCAGG GCACCGACAT CCGCGTGGCC GGTGAGAGCG CCAAGTTCGG GATCTCCGAG GCCAAGTGGA GCCTGTACCC GATGGGCGGT TCGGCCGTGC GCCTGGTGCG CCAGATCCCC TACACCATCG CGTGCGACAT GCTGCTCACC GGTCGCCACA TCACCGCGGC CGAGGCCAAG GAGTACGGCC TGATCGGGTA CGTCGTTCCC GACGGCACCG CGCTGGACAA GGCGCTGGAG ATCGCCGAGA CCATCAACAA CAACGGTCCG CTCGCCGTGC AGGCGATCCT CAAGACCATC CGTGAGACCG AGGGCATGCA CGAGGACGAG GCGTTCGGCC CCGACACCCG CAACGGCATC CCGGTGTTCC TGTCCGAGGA CGCCAAGGAG GGGCCGCGTG CGTTCAAGGA GAAGCGCGCA CCGAACTTCC AGATGAAG
Details for MysmA.01530.c.A1.GE28942
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMX XLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Validated NT Sequence
ttattacttc atctggaagt tcggtgcgcg cttctccttg aacgcacgcg gcccctcctt ggcgtcctcg gacaggaaca ccgggatgcc gttgcgggtg tcggggccga acgcctcgtc ctcgtgcatg ccctcggtct cacggatggt cttgaggatc gcctgcacgg cgagcggacc gttgttgttg atggtctcgg cgatctccag cgccttgtcc agcgcggtgc cgtcgggaac gacgtacccg atcaggccgt actccttggc ctcggccgcg gtgatgtggc gaccggtgag cagcatgtcg cacgcgatgg tgtaggggat ctggcgcacc aggcgcacgg ccgaaccgcc catcgggtac aggctccact tggcctcgga gatcccgaac ttggcgctct caccggccac gcggatgtcg gtgccctgca ggatctcggt gccgcccgcg atggcgggac cctcgaccgc ggcgatcagc ggcttggtca gacggcggcc cttgagcagg cctggaatct tcgacgggtc gtagctgccg tccttgaacg agtcacccgg cggcttcttg tccgcgccct tgaggtccat gcccgagcag aagtaaccgc cggcgccggt caggatgcag gtgcggatct ccggatcgtt gtcgacccgg tcccatgctt cgaccatgat cgagagcatc tcgcccgaga gagcgttgcg cgcctcagga cggttcaacg tgacgatgag ggtgtgtccg cgctgctcaa cgagggcgtc aggccccttg ccggtctcac tcaccatcga accaggaccc tgggtctgag cttccagggn nnccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgag accggcaagg ggcctgacgc cctcgttgag cagcgcggac acaccctcat cgtcacgttg aaccgtcctg aggcgcgcaa cgctctctcg ggcgagatgc tctcgatcat ggtcgaagca tgggaccggg tcgacaacga tccggagatc cgcacctgca tcctgaccgg cgccggcggt tacttctgct cgggcatgga cctcaagggc gcggacaaga agccgccggg tgactcgttc aaggacggca gctacgaccc gtcgaagatt ccaggcctgc tcaagggccg ccgtctgacc aagccgctga tcgccgcggt cgagggtccc gccatcgcgg gcggcaccga gatcctgcag ggcaccgaca tccgcgtggc cggtgagagc gccaagttcg ggatctccga ggccaagtgg agcctgtacc cgatgggcgg ttcggccgtg cgcctggtgc gccagatccc ctacaccatc gcgtgcgaca tgctgctcac cggtcgccac atcaccgcgg ccgaggccaa ggagtacggc ctgatcgggt acgtcgttcc cgacggcacc gcgctggaca aggcgctgga gatcgccgag accatcaaca acaacggtcc gctcgccgtg caggcgatcc tcaagaccat ccgtgagacc gagggcatgc acgaggacga ggcgttcggc cccgacaccc gcaacggcat cccggtgttc ctgtccgagg acgccaagga ggggccgcgt gcgttcaagg agaagcgcgc accgaacttc cagatgaaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.01530.c.A1.PS00771
PURIFICATION DATe: 9/16/2010
CONCENTRATION: 32.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX XLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Validated NT Sequence
ttattacttc atctggaagt tcggtgcgcg cttctccttg aacgcacgcg gcccctcctt ggcgtcctcg gacaggaaca ccgggatgcc gttgcgggtg tcggggccga acgcctcgtc ctcgtgcatg ccctcggtct cacggatggt cttgaggatc gcctgcacgg cgagcggacc gttgttgttg atggtctcgg cgatctccag cgccttgtcc agcgcggtgc cgtcgggaac gacgtacccg atcaggccgt actccttggc ctcggccgcg gtgatgtggc gaccggtgag cagcatgtcg cacgcgatgg tgtaggggat ctggcgcacc aggcgcacgg ccgaaccgcc catcgggtac aggctccact tggcctcgga gatcccgaac ttggcgctct caccggccac gcggatgtcg gtgccctgca ggatctcggt gccgcccgcg atggcgggac cctcgaccgc ggcgatcagc ggcttggtca gacggcggcc cttgagcagg cctggaatct tcgacgggtc gtagctgccg tccttgaacg agtcacccgg cggcttcttg tccgcgccct tgaggtccat gcccgagcag aagtaaccgc cggcgccggt caggatgcag gtgcggatct ccggatcgtt gtcgacccgg tcccatgctt cgaccatgat cgagagcatc tcgcccgaga gagcgttgcg cgcctcagga cggttcaacg tgacgatgag ggtgtgtccg cgctgctcaa cgagggcgtc aggccccttg ccggtctcac tcaccatcga accaggaccc tgggtctgag cttccagggn nnccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTGAG ACCGGCAAGG GGCCTGACGC CCTCGTTGAG CAGCGCGGAC ACACCCTCAT CGTCACGTTG AACCGTCCTG AGGCGCGCAA CGCTCTCTCG GGCGAGATGC TCTCGATCAT GGTCGAAGCA TGGGACCGGG TCGACAACGA TCCGGAGATC CGCACCTGCA TCCTGACCGG CGCCGGCGGT TACTTCTGCT CGGGCATGGA CCTCAAGGGC GCGGACAAGA AGCCGCCGGG TGACTCGTTC AAGGACGGCA GCTACGACCC GTCGAAGATT CCAGGCCTGC TCAAGGGCCG CCGTCTGACC AAGCCGCTGA TCGCCGCGGT CGAGGGTCCC GCCATCGCGG GCGGCACCGA GATCCTGCAG GGCACCGACA TCCGCGTGGC CGGTGAGAGC GCCAAGTTCG GGATCTCCGA GGCCAAGTGG AGCCTGTACC CGATGGGCGG TTCGGCCGTG CGCCTGGTGC GCCAGATCCC CTACACCATC GCGTGCGACA TGCTGCTCAC CGGTCGCCAC ATCACCGCGG CCGAGGCCAA GGAGTACGGC CTGATCGGGT ACGTCGTTCC CGACGGCACC GCGCTGGACA AGGCGCTGGA GATCGCCGAG ACCATCAACA ACAACGGTCC GCTCGCCGTG CAGGCGATCC TCAAGACCAT CCGTGAGACC GAGGGCATGC ACGAGGACGA GGCGTTCGGC CCCGACACCC GCAACGGCAT CCCGGTGTTC CTGTCCGAGG ACGCCAAGGA GGGGCCGCGT GCGTTCAAGG AGAAGCGCGC ACCGAACTTC CAGATGAAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MysmA.01530.c.A1.PW30072
PURIFICATION DATe: 8/2/2010
CONCENTRATION: 23.66mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX XLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Validated NT Sequence
ttattacttc atctggaagt tcggtgcgcg cttctccttg aacgcacgcg gcccctcctt ggcgtcctcg gacaggaaca ccgggatgcc gttgcgggtg tcggggccga acgcctcgtc ctcgtgcatg ccctcggtct cacggatggt cttgaggatc gcctgcacgg cgagcggacc gttgttgttg atggtctcgg cgatctccag cgccttgtcc agcgcggtgc cgtcgggaac gacgtacccg atcaggccgt actccttggc ctcggccgcg gtgatgtggc gaccggtgag cagcatgtcg cacgcgatgg tgtaggggat ctggcgcacc aggcgcacgg ccgaaccgcc catcgggtac aggctccact tggcctcgga gatcccgaac ttggcgctct caccggccac gcggatgtcg gtgccctgca ggatctcggt gccgcccgcg atggcgggac cctcgaccgc ggcgatcagc ggcttggtca gacggcggcc cttgagcagg cctggaatct tcgacgggtc gtagctgccg tccttgaacg agtcacccgg cggcttcttg tccgcgccct tgaggtccat gcccgagcag aagtaaccgc cggcgccggt caggatgcag gtgcggatct ccggatcgtt gtcgacccgg tcccatgctt cgaccatgat cgagagcatc tcgcccgaga gagcgttgcg cgcctcagga cggttcaacg tgacgatgag ggtgtgtccg cgctgctcaa cgagggcgtc aggccccttg ccggtctcac tcaccatcga accaggaccc tgggtctgag cttccagggn nnccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSETGKGP DALVEQRGHT LIVTLNRPEA RNALSGEMLS IMVEAWDRVD NDPEIRTCIL TGAGGYFCSG MDLKGADKKP PGDSFKDGSY DPSKIPGLLK GRRLTKPLIA AVEGPAIAGG TEILQGTDIR VAGESAKFGI SEAKWSLYPM GGSAVRLVRQ IPYTIACDML LTGRHITAAE AKEYGLIGYV VPDGTALDKA LEIAETINNN GPLAVQAILK TIRETEGMHE DEAFGPDTRN GIPVFLSEDA KEGPRAFKEK RAPNFQMK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTGAG ACCGGCAAGG GGCCTGACGC CCTCGTTGAG CAGCGCGGAC ACACCCTCAT CGTCACGTTG AACCGTCCTG AGGCGCGCAA CGCTCTCTCG GGCGAGATGC TCTCGATCAT GGTCGAAGCA TGGGACCGGG TCGACAACGA TCCGGAGATC CGCACCTGCA TCCTGACCGG CGCCGGCGGT TACTTCTGCT CGGGCATGGA CCTCAAGGGC GCGGACAAGA AGCCGCCGGG TGACTCGTTC AAGGACGGCA GCTACGACCC GTCGAAGATT CCAGGCCTGC TCAAGGGCCG CCGTCTGACC AAGCCGCTGA TCGCCGCGGT CGAGGGTCCC GCCATCGCGG GCGGCACCGA GATCCTGCAG GGCACCGACA TCCGCGTGGC CGGTGAGAGC GCCAAGTTCG GGATCTCCGA GGCCAAGTGG AGCCTGTACC CGATGGGCGG TTCGGCCGTG CGCCTGGTGC GCCAGATCCC CTACACCATC GCGTGCGACA TGCTGCTCAC CGGTCGCCAC ATCACCGCGG CCGAGGCCAA GGAGTACGGC CTGATCGGGT ACGTCGTTCC CGACGGCACC GCGCTGGACA AGGCGCTGGA GATCGCCGAG ACCATCAACA ACAACGGTCC GCTCGCCGTG CAGGCGATCC TCAAGACCAT CCGTGAGACC GAGGGCATGC ACGAGGACGA GGCGTTCGGC CCCGACACCC GCAACGGCAT CCCGGTGTTC CTGTCCGAGG ACGCCAAGGA GGGGCCGCGT GCGTTCAAGG AGAAGCGCGC ACCGAACTTC CAGATGAAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A