SSGCID: Target Page

MyulA.01530.g

Enoyl-CoA hydratase EchA19

CENTER ID:	MyulA.01530.g
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01530.g.A1.GE29901	Full length( MyulA.01530.g )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01530.g.A1.PW29767	Full length( MyulA.01530.g )		1	284

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.4739
RefSeq:	YP_907596.1
UniProt:	A0PUV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPDVMRKAVL VAPNTSETPA NGESGPDALV EQRGHTLIVT MNRPSRRNAL SGEMMQIMVE AWDRVDNDPD IRCCILTGAG DYFCAGMDLK AATKKPPGDS FKDGSYDPSR IDALLKGRRL KKPLIAAVEG PAIAGGTEIL QGTDIRVAAE SAKFGISEAE WSLYPMGGSA VRLVRQIPYT VACDLLLTGR HITAAEAKEM GLVGHVVPDG QALTKALEIA EIIAANGPLA VQAILRTIRE TEGMHENEAF KIDTRIGIEV FLSDDAKEGP QAFAQKRKPN FQNR

NT Sequence

ATGCCCGATG TCATGCGAAA GGCGGTGCTC GTGGCCCCGA ATACTTCCGA GACGCCAGCA AACGGGGAGT CCGGACCAGA CGCGCTGGTG GAGCAGCGCG GGCACACCCT GATCGTGACG ATGAATCGCC CGTCCCGACG TAACGCGCTT AGTGGCGAAA TGATGCAAAT CATGGTCGAG GCCTGGGATC GCGTCGACAA CGATCCCGAC ATCCGCTGCT GCATCCTGAC CGGCGCGGGC GACTACTTCT GCGCTGGGAT GGACCTCAAG GCGGCAACCA AGAAGCCGCC GGGCGACTCA TTCAAGGACG GCAGCTACGA CCCGTCGCGC ATCGACGCGC TGTTGAAGGG CCGCCGGCTG AAAAAGCCGC TGATCGCCGC CGTCGAGGGC CCCGCGATCG CCGGCGGAAC CGAGATTCTG CAAGGTACCG ACATTCGCGT CGCCGCCGAG AGCGCCAAGT TCGGCATCTC GGAGGCCGAG TGGAGCCTGT ACCCGATGGG CGGCTCGGCG GTGCGGCTGG TCCGCCAGAT CCCCTACACG GTGGCCTGCG ATCTGCTGCT GACCGGACGG CACATCACTG CCGCGGAGGC CAAGGAGATG GGGCTGGTGG GCCACGTCGT GCCCGACGGC CAGGCGCTGA CCAAGGCGCT GGAAATTGCG GAGATCATCG CGGCCAACGG GCCTCTTGCG GTCCAGGCGA TCTTGCGCAC CATCCGCGAA ACCGAGGGCA TGCACGAGAA CGAGGCGTTC AAGATCGACA CTCGAATCGG CATCGAGGTG TTCTTGTCCG ACGACGCCAA AGAAGGTCCG CAGGCGTTCG CGCAGAAGCG CAAACCCAAC TTCCAGAACC GC

Details for MyulA.01530.g.A1.GE29901

HARVESTED ON:	7/20/2010
SEQUENCED ON:	8/2/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	92

Validated AA Sequence

MPDVMRKXVL VAPNTSETPA NGESGPDALV EQRGHTLIVT MNRPSRRNAL SGEMMQIMVE AWDRVDNDPD IRCCILTGAG DYFCAGMDLK AATKKPPGDS FKDGSYDPSR IDALLKGRRL KKPLIAAVEG PAIAGGTEIL QGTDIRVAAE SAKFGISEAE WSLYPMGGSA VRLVRQIPYT VACDLLLTGR HITAAEAKEM GLVGHVVPDG QALTKALEIA EIIAANGPLA VQAILRTIRE TEGMHENEAF KIDTRIGIEV FLSDDAKEGP QAFAQKRKPN

Validated NT Sequence

aagttgggtt tgcgcttctg cgcgaacgcc tgcggacctt ctttggcgtc gtcggacaag aacacctcga tgccgattcg agtgtcgatc ttgaacgcct cgttctcgtg catgccctcg gtttcgcgga tggtgcgcaa gatcgcctgg accgcaagag gcccgttggc cgcgatgatc tccgcaattt ccagcgcctt ggtcagcgcc tggccgtcgg gcacgacgtg gcccaccagc cccatctcct tggcctccgc ggcagtgatg tgccgtccgg tcagcagcag atcgcaggcc accgtgtagg ggatctggcg gaccagccgc accgccgagc cgcccatcgg gtacaggctc cactcggcct ccgagatgcc gaacttggcg ctctcggcgg cgacgcgaat gtcggtacct tgcagaatct cggttccgcc ggcgatcgcg gggccctcga cggcggcgat cagcggcttt ttcagccggc ggcccttcaa cagcgcgtcg atgcgcgacg ggtcgtagct gccgtccttg aatgagtcgc ccggcggctt cttggttgcc gccttgaggt ccatcccagc gcagaagtag tcgcccgcgc cggtcaggat gcagcagcgg atgtcgggat cgttgtcgac gcgatcccag gcctcgacca tgatttgcat catttcgcca ctaagcgcgt tacgtcggga cgggcgattc atcgtcacga tcagggtgtg cccgcgctgc tccaccagcg cgtctggtcc ggactccccg tttgctggcg tctcggaagt attcggggcc acgagcaccg nctttcgcat gacatcgggc atcgaacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPDVMRKAV LVAPNTSETP ANGESGPDAL VEQRGHTLIV TMNRPSRRNA LSGEMMQIMV EAWDRVDNDP DIRCCILTGA GDYFCAGMDL KAATKKPPGD SFKDGSYDPS RIDALLKGRR LKKPLIAAVE GPAIAGGTEI LQGTDIRVAA ESAKFGISEA EWSLYPMGGS AVRLVRQIPY TVACDLLLTG RHITAAEAKE MGLVGHVVPD GQALTKALEI AEIIAANGPL AVQAILRTIR ETEGMHENEA FKIDTRIGIE VFLSDDAKEG PQAFAQKRKP NFQN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgatgtc atgcgaaagg cggtgctcgt ggccccgaat acttccgaga cgccagcaaa cggggagtcc ggaccagacg cgctggtgga gcagcgcggg cacaccctga tcgtgacgat gaatcgcccg tcccgacgta acgcgcttag tggcgaaatg atgcaaatca tggtcgaggc ctgggatcgc gtcgacaacg atcccgacat ccgctgctgc atcctgaccg gcgcgggcga ctacttctgc gctgggatgg acctcaaggc ggcaaccaag aagccgccgg gcgactcatt caaggacggc agctacgacc cgtcgcgcat cgacgcgctg ttgaagggcc gccggctgaa aaagccgctg atcgccgccg tcgagggccc cgcgatcgcc ggcggaaccg agattctgca aggtaccgac attcgcgtcg ccgccgagag cgccaagttc ggcatctcgg aggccgagtg gagcctgtac ccgatgggcg gctcggcggt gcggctggtc cgccagatcc cctacacggt ggcctgcgat ctgctgctga ccggacggca catcactgcc gcggaggcca aggagatggg gctggtgggc cacgtcgtgc ccgacggcca ggcgctgacc aaggcgctgg aaattgcgga gatcatcgcg gccaacgggc ctcttgcggt ccaggcgatc ttgcgcacca tccgcgaaac cgagggcatg cacgagaacg aggcgttcaa gatcgacact cgaatcggca tcgaggtgtt cttgtccgac gacgccaaag aaggtccgca ggcgttcgcg cagaagcgca aacccaactt ccagaacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MyulA.01530.g.A1.PW29767

PURIFICATION DATe:	9/15/2010
CONCENTRATION:	46.43mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGATGTC ATGCGAAAGG CGGTGCTCGT GGCCCCGAAT ACTTCCGAGA CGCCAGCAAA CGGGGAGTCC GGACCAGACG CGCTGGTGGA GCAGCGCGGG CACACCCTGA TCGTGACGAT GAATCGCCCG TCCCGACGTA ACGCGCTTAG TGGCGAAATG ATGCAAATCA TGGTCGAGGC CTGGGATCGC GTCGACAACG ATCCCGACAT CCGCTGCTGC ATCCTGACCG GCGCGGGCGA CTACTTCTGC GCTGGGATGG ACCTCAAGGC GGCAACCAAG AAGCCGCCGG GCGACTCATT CAAGGACGGC AGCTACGACC CGTCGCGCAT CGACGCGCTG TTGAAGGGCC GCCGGCTGAA AAAGCCGCTG ATCGCCGCCG TCGAGGGCCC CGCGATCGCC GGCGGAACCG AGATTCTGCA AGGTACCGAC ATTCGCGTCG CCGCCGAGAG CGCCAAGTTC GGCATCTCGG AGGCCGAGTG GAGCCTGTAC CCGATGGGCG GCTCGGCGGT GCGGCTGGTC CGCCAGATCC CCTACACGGT GGCCTGCGAT CTGCTGCTGA CCGGACGGCA CATCACTGCC GCGGAGGCCA AGGAGATGGG GCTGGTGGGC CACGTCGTGC CCGACGGCCA GGCGCTGACC AAGGCGCTGG AAATTGCGGA GATCATCGCG GCCAACGGGC CTCTTGCGGT CCAGGCGATC TTGCGCACCA TCCGCGAAAC CGAGGGCATG CACGAGAACG AGGCGTTCAA GATCGACACT CGAATCGGCA TCGAGGTGTT CTTGTCCGAC GACGCCAAAG AAGGTCCGCA GGCGTTCGCG CAGAAGCGCA AACCCAACTT CCAGAACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA