SSGCID: Target Page

MyulA.01530.d

Enoyl-CoA hydratase

CENTER ID:	MyulA.01530.d
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01530.d.A1.GE29898	Full length( MyulA.01530.d )		1	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.1378
RefSeq:	YP_905175.1
UniProt:	A0PMY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLERDGHVL LIGLNRPQKR NSFDRAMFAD LSRAYALLET DDSVRAGVLF AHGDHFTGGL DLVDVAPSIA AGQSPFPADG RDPWRLDGPW RTPLIAVAHG WCMTLGIELL LASDIRIAAA GTRFSQLEVQ RGIYPFGGAT IRLTRDAGWG NAMRWLLTGD EFDAAEAHRI GLVQEVADDA ATALTRAREI AQTIADRAAP LGVRATLASV HLARREGEAA AIERLVPDIR ALFTSADAAE GVQSFIERRQ ARFTGR

NT Sequence

GTGTCCCTCG AGCGGGATGG CCACGTCCTG CTGATCGGCC TGAATCGGCC GCAGAAACGC AATTCGTTCG ACCGGGCAAT GTTTGCTGAC CTGTCGCGCG CCTACGCGCT GCTGGAAACC GACGACTCGG TGCGGGCCGG CGTCCTGTTC GCCCATGGTG ACCACTTCAC CGGCGGCCTG GATCTGGTTG ACGTTGCGCC CAGCATCGCG GCCGGACAGT CCCCGTTCCC CGCGGATGGT CGCGACCCGT GGCGGCTGGA CGGCCCGTGG CGCACACCGC TGATCGCTGT CGCACACGGC TGGTGCATGA CGTTGGGCAT CGAATTGCTG CTGGCCTCCG ACATTCGCAT CGCGGCGGCC GGAACCCGAT TCAGTCAACT CGAAGTACAG CGCGGGATCT ACCCGTTCGG AGGCGCGACG ATCCGGCTGA CCCGTGACGC CGGGTGGGGT AACGCGATGC GCTGGTTGCT CACCGGCGAC GAGTTCGATG CCGCCGAAGC GCATCGCATC GGGCTGGTCC AAGAGGTGGC CGACGATGCG GCGACGGCTC TGACCCGGGC TCGGGAGATC GCGCAGACCA TCGCCGACCG TGCCGCACCG TTGGGCGTGC GAGCCACCCT CGCGTCGGTG CATCTGGCTC GCCGGGAAGG TGAGGCCGCG GCGATCGAGC GCCTGGTCCC CGATATTCGG GCGCTATTCA CCAGCGCAGA TGCCGCCGAG GGAGTGCAGT CTTTCATCGA ACGCCGACAG GCCCGATTCA CCGGCCGC

Details for MyulA.01530.d.A1.GE29898

HARVESTED ON:	7/20/2010
SEQUENCED ON:	8/2/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSLERDGH VLLIGLNRPQ KRNSFDRAMF ADLSRAYALL ETDDSVRAGV LFAHGDHFTG GLDLVDVAPS IAAGQSPFPA DGRDPWRLDG PWRTPLIAVA HGWCMTLGIE LLLASDIRIA AAGTRFSQLE VQRGIYPFGG ATIRLTRDAG WGNAMRWLLT GDEFDAAEAH RIGLVQEVAD DAATALTRAR EIAQTIADRA APLGVRATLA SVHLARREGE AAAIERLVPD IRALFTSADA AEGVQSFIER RXARFTG

Validated NT Sequence

tttgttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgtcc ctcgagcggg atggccacgt cctgctgatc ggcctgaatc ggccgcagaa acgcaattcg ttcgaccggg caatgtttgc tgacctgtcg cgcgcctacg cgctgctgga aaccgacgac tcggtgcggg ccggcgtcct gttcgcccat ggtgaccact tcaccggcgg cctggatctg gttgacgttg cgcccagcat cgcggccgga cagtccccgt tccccgcgga tggtcgcgac ccgtggcggc tggacggccc gtggcgcaca ccgctgatcg ctgtcgcaca cggctggtgc atgacgttgg gcatcgaatt gctgctggcc tccgacattc gcatcgcggc ggccggaacc cgattcagtc aactcgaagt acagcgcggg atctacccgt tcggaggcgc gacgatccgg ctgacccgtg acgccgggtg gggtaacgcg atgcgctggt tgctcaccgg cgacgagttc gatgccgccg aagcgcatcg catcgggctg gtccaagagg tggccgacga tgcggcgacg gctctgaccc gggctcggga gatcgcgcag accatcgccg accgtgccgc accgttgggc gtgcgagcca ccctcgcgtc ggtgcatctg gctcgccggg aaggtgaggc cgcggcgatc gagcgcctgg tccccgatat tcgggcgcta ttcaccagcg cagatgccgc cgagggagtg cagtctttca tcgaacgccg acnggcccga ttcaccggnt aataanagca cgaanaagtt ctgcagccaa gcttctcgag gatccngctg ctnacaaagc nnganaggaa gctnanttgg ctgctgccnc cgctgnnnnn ntnantannn aaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccctc gagcgggatg gccacgtcct gctgatcggc ctgaatcggc cgcagaaacg caattcgttc gaccgggcaa tgtttgctga cctgtcgcgc gcctacgcgc tgctggaaac cgacgactcg gtgcgggccg gcgtcctgtt cgcccatggt gaccacttca ccggcggcct ggatctggtt gacgttgcgc ccagcatcgc ggccggacag tccccgttcc ccgcggatgg tcgcgacccg tggcggctgg acggcccgtg gcgcacaccg ctgatcgctg tcgcacacgg ctggtgcatg acgttgggca tcgaattgct gctggcctcc gacattcgca tcgcggcggc cggaacccga ttcagtcaac tcgaagtaca gcgcgggatc tacccgttcg gaggcgcgac gatccggctg acccgtgacg ccgggtgggg taacgcgatg cgctggttgc tcaccggcga cgagttcgat gccgccgaag cgcatcgcat cgggctggtc caagaggtgg ccgacgatgc ggcgacggct ctgacccggg ctcgggagat cgcgcagacc atcgccgacc gtgccgcacc gttgggcgtg cgagccaccc tcgcgtcggt gcatctggct cgccgggaag gtgaggccgc ggcgatcgag cgcctggtcc ccgatattcg ggcgctattc accagcgcag atgccgccga gggagtgcag tctttcatcg aacgccgaca ggcccgattc accggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa