SSGCID: Target Page

MypaA.01530.e

EchA19

CENTER ID:	MypaA.01530.e
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01530.e.A1.GE29612	Full length( MypaA.01530.e )		1	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.581
RefSeq:	NP_959483.1
UniProt:	Q743P2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEASANEKT GPDALVEQRG HTLIVTLNRP HARNALSTEM MEIMVQAWDR VDNDDDIRCC ILTGAGGYFC AGMDLKAATK KPPGESFKDG SYDPSRIDAL LKGRRLTKPL IAAVEGPAIA GGTEILQGTD IRVAGESAKF GISEAKWSLY PMGGSAVRLV RQIPYTVACD LLLTGRHITA AEAKEMGLIG HVVPDGQALA KALEIAEVIE NNGPLAVQAI LRAIRETEGM HENEAFKIDT QIGIKVFLSE DAKEGPRAFA EKRKPNFQNR

NT Sequence

GTGGCCGAGG CGTCAGCAAA CGAAAAAACC GGTCCCGACG CGCTGGTCGA GCAGCGCGGT CACACCCTCA TCGTGACGCT AAACCGGCCG CACGCGCGCA ACGCGCTGAG CACCGAAATG ATGGAGATCA TGGTGCAGGC CTGGGACCGC GTCGACAACG ACGACGACAT CCGCTGCTGC ATCCTGACCG GCGCCGGTGG CTACTTCTGC GCCGGCATGG ACCTCAAGGC GGCGACCAAG AAACCGCCGG GCGAATCCTT CAAGGACGGC AGCTACGACC CGTCGCGAAT CGATGCCCTG CTGAAGGGCC GGCGGCTGAC CAAGCCGTTG ATCGCGGCCG TCGAGGGCCC CGCGATCGCC GGCGGCACCG AGATCCTGCA GGGCACCGAC ATCCGGGTGG CCGGCGAGAG CGCCAAGTTC GGCATCTCGG AGGCCAAGTG GAGCCTGTAC CCGATGGGCG GCTCGGCGGT GCGGCTGGTG CGCCAGATCC CCTACACGGT GGCCTGCGAC CTGCTGCTGA CCGGGCGGCA CATCACCGCC GCCGAGGCCA AGGAGATGGG GCTGATCGGG CACGTCGTCC CCGACGGGCA GGCGCTGGCC AAGGCCCTCG AGATCGCCGA GGTGATCGAG AACAACGGGC CGCTGGCCGT GCAGGCGATC CTGCGAGCGA TCCGCGAGAC CGAGGGCATG CACGAGAACG AGGCGTTCAA GATCGACACG CAGATCGGCA TCAAGGTGTT CCTGTCCGAG GACGCCAAGG AAGGCCCGCG CGCATTCGCC GAGAAGCGCA AACCCAACTT CCAAAACCGC

Details for MypaA.01530.e.A1.GE29612

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	31kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAEASANE KTGPDALVEQ RGHTLIVTLN RPHARNALST EMMEIMVQAW DRVDNDDDIR CCILTGAGGY FCAGMDLKAA TKKPPGESFK DGSYDPSRID ALLKGRRLTK PLIAAVEGPA IAGGTEILQG TDIRVAGESA KFGISEAKWS LYPMGGSAVR LVRQIPYTVA CDLLLTGRHI TAAEAKEMGL IGHVVPDGQA LAKALEIAEV IENNGPLAVQ AILRAIRETE GMHENEAFKI DTQIGIKVFL SEDAKEXPAR IRRKRKPNFQ NR

Validated NT Sequence

ttttgtttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ccgaggcgtc agcaaacgaa aaaaccggtc ccgacgcgct ggtcgagcag cgcggtcaca ccctcatcgt gacgctaaac cggccgcacg cgcgcaacgc gctgagcacc gaaatgatgg agatcatggt gcaggcctgg gaccgcgtcg acaacgacga cgacatccgc tgctgcatcc tgaccggcgc cggtggctac ttctgcgccg gcatggacct caaggcggcg accaagaaac cgccgggcga atccttcaag gacggcagct acgacccgtc gcgaatcgat gccctgctga agggccggcg gctgaccaag ccgttgatcg cggccgtcga gggccccgcg atcgccggcg gcaccgagat cctgcagggc accgacatcc gggtggccgg cgagagcgcc aagttcggca tctcggaggc caagtggagc ctgtacccga tgggcggctc ggcggtgcgg ctggtgcgcc agatccccta cacggtggcc tgcgacctgc tgctgaccgg gcggcacatc accgccgccg aggccaagga gatggggctg atcgggcacg tcgtccccga cgggcaggcg ctggccaagg ccctcgagat cgccgaggtg atcgagaaca acgggccgct ggccgtgcag gcgatcctgc gagcgatccg cgagaccgag ggcatgcacg agaacgaggc gttcaagatc gacacgcaga tcggcatcaa ggtgttcctg tccgaggacg ccaaggaang gcccgcgcgc attcgccgaa agcgcaaacc caacttccaa aaccgctaat aaacagcacg aacaagtnct gcagccaagc ttctcgagga tccgnntgct aacnaagccc gaaaggaagc tganttggnt gntgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgag gcgtcagcaa acgaaaaaac cggtcccgac gcgctggtcg agcagcgcgg tcacaccctc atcgtgacgc taaaccggcc gcacgcgcgc aacgcgctga gcaccgaaat gatggagatc atggtgcagg cctgggaccg cgtcgacaac gacgacgaca tccgctgctg catcctgacc ggcgccggtg gctacttctg cgccggcatg gacctcaagg cggcgaccaa gaaaccgccg ggcgaatcct tcaaggacgg cagctacgac ccgtcgcgaa tcgatgccct gctgaagggc cggcggctga ccaagccgtt gatcgcggcc gtcgagggcc ccgcgatcgc cggcggcacc gagatcctgc agggcaccga catccgggtg gccggcgaga gcgccaagtt cggcatctcg gaggccaagt ggagcctgta cccgatgggc ggctcggcgg tgcggctggt gcgccagatc ccctacacgg tggcctgcga cctgctgctg accgggcggc acatcaccgc cgccgaggcc aaggagatgg ggctgatcgg gcacgtcgtc cccgacgggc aggcgctggc caaggccctc gagatcgccg aggtgatcga gaacaacggg ccgctggccg tgcaggcgat cctgcgagcg atccgcgaga ccgagggcat gcacgagaac gaggcgttca agatcgacac gcagatcggc atcaaggtgt tcctgtccga ggacgccaag gaaggcccgc gcgcattcgc cgagaagcgc aaacccaact tccaaaaccg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa