MytuD.00760.a

Uncharacterized protein Rv1708/MT1749

CENTER ID: MytuD.00760.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00760.a.B1.GE37756 full length 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1901
RefSeq: NP_216224.1
RefSeq: YP_006515103.1
TubercuList: Rv1708
MTB Network Portal: Rv1708
UniProt: P9WLT1

Orthologues for Rv1708

Species % Identity % Coverage Clones Proteins Structures
avium 90 90 1 1 0
leprae 90 90 1 0 0
marinum 89 100 1 0 0
ulcerans 89 100 0 0 0
fortuitum 89 87 1 2 0
smegmatis 89 87 1 1 0
thermoresistibile 86 90 1 0 0
paratuberculosis 85 100 1 0 0
abscessus 79 89 1 1 0
marinum 26 65 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAGLPGQAS VAVRLSCDVP PDARHHEPRP GMTDHPDTGN GIGLTGRPPR AIPDPAPRSS HGPAKVIAMC NQKGGVGKTT STINLGAALG EYGRRVLLVD MDPQGALSAG LGVPHYELDK TIHNVLVEPR VSIDDVLIHS RVKNMDLVPS NIDLSAAEIQ LVNEVGREQT LARALYPVLD RYDYVLIDCQ PSLGLLTVNG LACTDGVIIP TECEFFSLRG LALLTDTVDK VRDRLNPKLD ISGILITRYD PRTVNSREVM ARVVERFGDL VFDTVITRTV RFPETSVAGE PITTWAPKSA GALAYRALAR ELIDRFGM
NT Sequence
ttgcctgcgg gtctcccggg ccaggcgtcg gtagccgtta gactttcctg cgatgtcccc cctgacgccc gtcaccacga gccacgaccg ggtatgaccg accaccccga caccggcaac gggatcggcc tcaccggacg gccaccacgg gcaatccctg accccgcgcc gcgcagctcg cacggcccgg ccaaggtcat cgcgatgtgc aaccagaagg gtggcgtcgg gaagacgacg tcgacgatta acctgggtgc cgcgctcggt gagtatggcc ggcgggtgct gctggtggat atggatccgc aaggagcgct gtccgcgggc ctgggcgtgc cgcactacga gctggacaag accatccaca acgtgctggt ggagccccgg gtgtcgatcg acgacgtgct gatccactcc cgggtgaaaa acatggatct ggtccccagc aatatcgatc tgtccgcggc ggagatccaa ctggtcaacg aggtgggtcg cgagcagacg ttggcccggg cgctgtaccc ggtgctggac cgctacgact atgtgctgat cgactgccag ccgtcgctgg gcctgctcac cgtcaacggg ctggcctgca cggacggcgt gataattccg accgagtgcg agttcttctc gctgcgcggc ctggcattgc tcaccgacac cgtcgataag gtgcgcgacc ggcttaatcc gaagctggat atcagcggaa tcctgatcac ccgctacgat ccgcggaccg tcaactcgcg agaggtcatg gcccgtgtcg tggaacggtt cggtgactta gtgtttgaca ccgtgatcac ccgcacggtt cgtttcccgg agaccagcgt cgcaggcgaa cccattacca cctgggcgcc gaagtcggcg ggtgccctgg cctaccgtgc gctggctcgc gagttgatcg accgatttgg catgtga
Details for MytuD.00760.a.B1.GE37756
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MLPAGLPGQA SVAVRLSCDV PPDARHHEPR PGMTDHPDTG NGIGLTGRPP RAIPDPAPRS SHGPAKVIAM CNQKGGVGKT TSTINLGAAL GEYGRRVLLV DMDPQGALSA GLGVPHYELD KTIHNVLVEP RVSIDDVLIH SRVKNMDLVP SNIDLSAAEI QLVNEVGREQ TLARALYPVL DRYDYVLIDC QPSLGLLTVN GLACTDGVII PTECEFFSLR GLALLTDTVD KVRDRLNPKL DISGILITRY DPRTVNSREV MARVVERFGD LVFDTVITRT VRFPETSVAG EPITTWAPKS AGALAYRALA RELIDRFGM
Validated NT Sequence
tcttactcac ttacatgcca aatcggtcga tcaactcgcg agccagcgca cggtaggcca gggcacccgc cgacttcggc gcccaggtgg taatgggttc gcctgcgacg ctggtctccg ggaaacgaac cgtgcgggtg atcacggtgt caaacactaa gtcaccgaac cgttccacga cacgggccat gacctctcgc gagttgacgg tccgcggatc gtagcgggtg atcaggattc cgctgatatc cagcttcgga ttaagccggt cgcgcacctt atcgacggtg tcggtgagca atgccaggcc gcgcagcgag aagaactcgc actcggtcgg aattatcacg ccgtccgtgc aggccagccc gttgacggtg agcaggccca gcgacggctg gcagtcgatc agcacatagt cgtagcggtc cagcaccggg tacagcgccc gggccaacgt ctgctcgcga cccacctcgt tgaccagttg gatctccgcc gcggacagat cgatattgct ggggaccaga tccatgtttt tcacccggga gtggatcagc acgtcgtcga tcgacacccg gggctccacc agcacgttgt ggatggtctt gtccagctcg tagtgcggca cgcccaggcc cgcggacagc gctccttgcg gatccatatc caccagcagc acccgccggc catactcacc gagcgcggca cccaggttaa tcgtcgacgt cgtcttcccg acgccaccct tctggttgca catcgcgatg accttggccg ggccgtgcga gctgcgcggc gcggggtcag ggattgcccg tggtggccgt ccggtgaggc cgatcccgtt gccggtgtcg gggtggtcgg tcatacccgg tcgtggctcg tggtgacggg cgtcaggggg gacatcgcag gaaagtctaa cggctaccga cgcctggccc gggagacccg caggcaacat atg
Expected Protein Sequence
MAHHHHHHMP AGLPGQASVA VRLSCDVPPD ARHHEPRPGM TDHPDTGNGI GLTGRPPRAI PDPAPRSSHG PAKVIAMCNQ KGGVGKTTST INLGAALGEY GRRVLLVDMD PQGALSAGLG VPHYELDKTI HNVLVEPRVS IDDVLIHSRV KNMDLVPSNI DLSAAEIQLV NEVGREQTLA RALYPVLDRY DYVLIDCQPS LGLLTVNGLA CTDGVIIPTE CEFFSLRGLA LLTDTVDKVR DRLNPKLDIS GILITRYDPR TVNSREVMAR VVERFGDLVF DTVITRTVRF PETSVAGEPI TTWAPKSAGA LAYRALAREL IDRFGM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcctg cgggtctccc gggccaggcg tcggtagccg ttagactttc ctgcgatgtc ccccctgacg cccgtcacca cgagccacga ccgggtatga ccgaccaccc cgacaccggc aacgggatcg gcctcaccgg acggccacca cgggcaatcc ctgaccccgc gccgcgcagc tcgcacggcc cggccaaggt catcgcgatg tgcaaccaga agggtggcgt cgggaagacg acgtcgacga ttaacctggg tgccgcgctc ggtgagtatg gccggcgggt gctgctggtg gatatggatc cgcaaggagc gctgtccgcg ggcctgggcg tgccgcacta cgagctggac aagaccatcc acaacgtgct ggtggagccc cgggtgtcga tcgacgacgt gctgatccac tcccgggtga aaaacatgga tctggtcccc agcaatatcg atctgtccgc ggcggagatc caactggtca acgaggtggg tcgcgagcag acgttggccc gggcgctgta cccggtgctg gaccgctacg actatgtgct gatcgactgc cagccgtcgc tgggcctgct caccgtcaac gggctggcct gcacggacgg cgtgataatt ccgaccgagt gcgagttctt ctcgctgcgc ggcctggcat tgctcaccga caccgtcgat aaggtgcgcg accggcttaa tccgaagctg gatatcagcg gaatcctgat cacccgctac gatccgcgga ccgtcaactc gcgagaggtc atggcccgtg tcgtggaacg gttcggtgac ttagtgtttg acaccgtgat cacccgcacg gttcgtttcc cggagaccag cgtcgcaggc gaacccatta ccacctgggc gccgaagtcg gcgggtgccc tggcctaccg tgcgctggct cgcgagttga tcgaccgatt tggcatgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at