MymaA.00760.b

ATPase involved in chromosome partitioning (Soj family) (Rv3918c ortholog)

CENTER ID: MymaA.00760.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00760.b.B1.GE37193 full length 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2686
RefSeq: YP_001850829.1
UniProt: B2HRU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAGLPAQAD AAVRLSCDVH PDVLHNASRS GMNDHPDSGV SVGLTGRPPR AIPDPKPLSS HGPAKVVAMC NQKGGVGKTT STINLGAALA EYDRRVLLVD MDPQGALSAG LGVAHYELEK TIHNVLVEPR VSVDDVLIHT RVKNLDLVPS NIDLSAAEIQ LVNEVGREQT LGRALYPVLD RYDYVLIDCQ PSLGLLTVNG LACADGVVIP TECEYFSLRG LALLTDTVEK VRDRLNPRLD ISGILLTRYD PRTVNSREVM ARVVERFGDL VFDTVITRTV RFPETSVAGE PITTWAPKSA GALAYRVLAR EFIDRFGV
NT Sequence
ttgcctgcgg gtctcccggc gcaggcggac gcagccgtta gactttcctg cgatgtccac cctgacgtgc ttcacaacgc atcgcgatcg ggcatgaacg atcacccaga cagcggtgtc tcggtcggct tgaccggtag gccaccgcgg gctatcccag accccaagcc gctgagctca cacgggcccg ccaaggtcgt tgcgatgtgc aaccagaagg gcggggtggg caagaccacg tcgaccatca acctgggcgc cgcgctggcc gagtacgacc gacgagtact gctggtggac atggatcccc agggtgcgct gtcggcgggc ctgggagtgg cccactacga gctggaaaag accatccaca acgtgctggt ggagccccgg gtgtccgtcg atgacgtgct catccatacc cgggtgaaga acctggacct ggtgcccagc aacatcgacc tgtcggccgc cgagattcag ctggtcaacg aggtgggccg ggaacagacg ctggggcggg cgctctaccc ggtgctggat cgctacgact atgtgctgat cgactgccag ccgtccctgg ggctgcttac cgtcaatggg ctggcctgcg ccgacggtgt cgtcatcccg accgaatgcg agtacttctc gctgcgtggc ctggcgctgc tgaccgacac cgtggagaag gtgcgcgacc ggctgaaccc caggctggac atcagcggca tcctgctcac ccgctatgac ccgcgcacgg tcaactcccg ggaggtcatg gcccgcgtcg tcgaacgatt cggtgaccta gtgttcgata ccgtgatcac ccgcacggtt cgtttcccgg agaccagcgt cgccggcgaa cccattacga cgtgggcccc caagtccgct ggcgcgttgg cctatcgcgt gttggcccgc gagttcatcg accgattcgg cgtgtga
Details for MymaA.00760.b.B1.GE37193
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML PAGLPAQADA AVRLSCDVHP DVLHNASRSG MNDHPDSGVS VGLTGRPPRA IPDPKPLSSH GPAKVVAMCN QKGGVGKTTS TINLGAALAE YDRRVLLVDM DPQGALSAGL GVAHYELEKT IHNVLVEPRV SVDDVLIHTR VKNLDLVPSN IDLSAAEIQL VNEVGREQTL GRALYPVLDR YDYVLIDCQP SLGLLTVNGL ACADGVVIPT ECEYFSLRGL ALLTDTVEKV RDRLNPRLDI SGILLTRYDP RTVNSREVMA RVVERFGDLV FDTVITRTVR FPETSVAGEP ITTWAPKSAG ALAYRVLARE FI
Validated NT Sequence
gtcgatgaac tcgcgggcca acacgcgata ggccaacgcg ccagcggact tgggggccca cgtcgtaatg ggttcgccgg cgacgctggt ctccgggaaa cgaaccgtgc gggtgatcac ggtatcgaac actaggtcac cgaatcgttc gacgacgcgg gccatgacct cccgggagtt gaccgtgcgc gggtcatagc gggtgagcag gatgccgctg atgtccagcc tggggttcag ccggtcgcgc accttctcca cggtgtcggt cagcagcgcc aggccacgca gcgagaagta ctcgcattcg gtcgggatga cgacaccgtc ggcgcaggcc agcccattga cggtaagcag ccccagggac ggctggcagt cgatcagcac atagtcgtag cgatccagca ccgggtagag cgcccgcccc agcgtctgtt cccggcccac ctcgttgacc agctgaatct cggcggccga caggtcgatg ttgctgggca ccaggtccag gttcttcacc cgggtatgga tgagcacgtc atcgacggac acccggggct ccaccagcac gttgtggatg gtcttttcca gctcgtagtg ggccactccc aggcccgccg acagcgcacc ctggggatcc atgtccacca gcagtactcg tcggtcgtac tcggccagcg cggcgcccag gttgatggtc gacgtggtct tgcccacccc gcccttctgg ttgcacatcg caacgacctt ggcgggcccg tgtgagctca gcggcttggg gtctgggata gcccgcggtg gcctaccggt caagccgacc gagacaccgc tgtctgggtg atcgttcatg cccgatcgcg atgcgttgtg aagcacgtca gggtggacat cgcaggaaag tctaacggct gcgtccgcct gcgccgggag acccgcaggc aacatatggt ggtggtggtg gtgagccatg gtat
Expected Protein Sequence
MAHHHHHHMP AGLPAQADAA VRLSCDVHPD VLHNASRSGM NDHPDSGVSV GLTGRPPRAI PDPKPLSSHG PAKVVAMCNQ KGGVGKTTST INLGAALAEY DRRVLLVDMD PQGALSAGLG VAHYELEKTI HNVLVEPRVS VDDVLIHTRV KNLDLVPSNI DLSAAEIQLV NEVGREQTLG RALYPVLDRY DYVLIDCQPS LGLLTVNGLA CADGVVIPTE CEYFSLRGLA LLTDTVEKVR DRLNPRLDIS GILLTRYDPR TVNSREVMAR VVERFGDLVF DTVITRTVRF PETSVAGEPI TTWAPKSAGA LAYRVLAREF IDRFGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcctg cgggtctccc ggcgcaggcg gacgcagccg ttagactttc ctgcgatgtc caccctgacg tgcttcacaa cgcatcgcga tcgggcatga acgatcaccc agacagcggt gtctcggtcg gcttgaccgg taggccaccg cgggctatcc cagaccccaa gccgctgagc tcacacgggc ccgccaaggt cgttgcgatg tgcaaccaga agggcggggt gggcaagacc acgtcgacca tcaacctggg cgccgcgctg gccgagtacg accgacgagt actgctggtg gacatggatc cccagggtgc gctgtcggcg ggcctgggag tggcccacta cgagctggaa aagaccatcc acaacgtgct ggtggagccc cgggtgtccg tcgatgacgt gctcatccat acccgggtga agaacctgga cctggtgccc agcaacatcg acctgtcggc cgccgagatt cagctggtca acgaggtggg ccgggaacag acgctggggc gggcgctcta cccggtgctg gatcgctacg actatgtgct gatcgactgc cagccgtccc tggggctgct taccgtcaat gggctggcct gcgccgacgg tgtcgtcatc ccgaccgaat gcgagtactt ctcgctgcgt ggcctggcgc tgctgaccga caccgtggag aaggtgcgcg accggctgaa ccccaggctg gacatcagcg gcatcctgct cacccgctat gacccgcgca cggtcaactc ccgggaggtc atggcccgcg tcgtcgaacg attcggtgac ctagtgttcg ataccgtgat cacccgcacg gttcgtttcc cggagaccag cgtcgccggc gaacccatta cgacgtgggc ccccaagtcc gctggcgcgt tggcctatcg cgtgttggcc cgcgagttca tcgaccgatt cggcgtgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at