SSGCID: Target Page

MyfoA.11416.a

Cobyrinic acid a,c-diamide synthase (Rv3918c ortholog)

CENTER ID:	MyfoA.11416.a
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.11416.a.B1.GE40122	full length		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.11416.a.B1.PS37980	full length		1	294

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.2620
RefSeq:	WP_003884494.1
UniProt:	K0VQU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDDSAHDGL FEHSSPELGL TGRPPRDIPE PKARTVHGPA KVIAMCNQKG GVGKTTSTIN LGASLAEYGR RVLLVDLDPQ GALSAGLGVP HYELEHTVHN LLVEPRVSID DVVIKTRVSG MDLVPSNIDL SAAEIQLVNE VGREQSLARA LYPVLDRYDY VLIDCQPSLG LLTVNGLACA DGVIIPTECE YFSLRGLALL TDTVDKVHDR LNPKLSISGI LVTRYDPRTV NAREVMARVV ERFGDLVFDT VITRTVRFPE TSVAGEPITT WAPKSGGAIA YRSLAREVID RFGA

NT Sequence

atgagcgacg acagcgcaca cgacggcctc ttcgagcaca gctcacccga gctcggtttg accgggcggc cgccgcgcga catccccgaa ccgaaggccc gcacagtgca tggcccggcc aaggtcatcg cgatgtgcaa ccagaagggc ggggtcggta agaccacgtc gaccatcaac ctgggcgcca gcctggccga gtacggccgc cgcgtcctgc tggtggacct ggacccccaa ggcgcgctgt cggcgggcct cggcgtgccg cactacgaac tcgagcacac ggtgcacaac ctgctggtcg aaccgcgggt gtccatcgac gacgtcgtca tcaagacccg cgtcagcggc atggatctgg tgcccagcaa catcgacctg tcggccgcgg agatccagct ggtcaacgag gtgggccgcg agcagtccct ggcccgtgcc ctgtatccgg tgctggaccg ctacgactac gtgctgatcg actgccagcc gtcgctgggc ctgctgacag tcaacgggct ggcctgcgcc gacggtgtga tcatccccac cgaatgcgag tacttctcgc tgcgcgggct ggccctgctg accgacaccg tggacaaggt gcacgaccgg ctcaacccga agctgtcgat cagcggcatc ctggtgaccc gctacgaccc gcgcaccgtc aacgcccgcg aggtcatggc tcgcgtcgtg gagcggttcg gtgacctggt gttcgacacg gtgatcaccc ggaccgtgcg gttcccggag accagcgtgg ccggcgagcc gatcaccacc tgggcgccca agtcgggtgg cgccatcgcc tatcgatcgc tggcacgcga agtcatcgac cggttcggcg cgtga

Details for MyfoA.11416.a.B1.GE40122

HARVESTED ON:	4/6/2016
SEQUENCED ON:	4/13/2016
EXPECTED MW:	33kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS DDSAHDGLFE HSSPELGLTG RPPRDIPEPK ALTVHGPAKV IAMCNQKGGV GKTTSTINLG ASLAEYGRRV LLVDLDPQGA LSAGLGVPHY ELEHTVHNLL VEPRVSIDDV VIKTRVSGMD LVPSNIDLSA AEIQLVNEVG REQSLARALY PVLDRYDYVL IDCQPSLGLL TVNGLACAYG VIIPTECEYF SLRGLALLTD TVDKVHDRLN PKLSISGILV TRYDPRTVNA REVMARVVER FGDLVFDTVI TRTVRFPETS VAGEPITTWA PKSGGAIAYR SLAREXXDRF GA

Validated NT Sequence

tcttactcac ttacgcgccg aaccggtcgn nnnnttcgcg tgccagcgat cgataggcga tggcgccacc cgacttgggc gcccaggtgg tgatcggctc gccggccacg ctggtctccg ggaaccgcac ggtccgggtg atcaccgtgt cgaacaccag gtcaccgaac cgctccacga cgcgagccat gacctcgcgg gcgttgacgg tgcgcgggtc gtagcgggtc accaggatgc cgctgatcga cagcttcggg ttgagccggt cgtgcacctt gtccacggtg tcggtcagca gggccagccc gcgcagcgag aagtactcgc attcggtggg gatgatcaca ccgtaggcgc aggccagccc gttgactgtc agcaggccca gcgacggctg gcagtcgatc agcacgtagt cgtagcggtc cagcaccgga tacagggcac gggccaggga ctgctcgcgg cccacctcgt tgaccagctg gatctccgcg gccgacaggt cgatgttgct gggcaccaga tccatgccgc tgacgcgggt cttgatgacg acgtcgtcga tggacacccg cggttcgacc agcaggttgt gcaccgtgtg ctcgagttcg tagtgcggca cgccgaggcc cgccgacagc gcgccttggg ggtccaggtc caccagcagg acgcggcggc cgtactcggc caggctggcg cccaggttga tggtcgacgt ggtcttaccg accccgccct tctggttgca catcgcgatg accttggccg ggccatgcac tgtgagggcc ttcggttcgg ggatgtcgcg cggcggccgc ccggtcaaac cgagctcggg tgagctgtgc tcgaagaggc cgtcgtgtgc gctgtcgtcg ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS DDSAHDGLFE HSSPELGLTG RPPRDIPEPK ARTVHGPAKV IAMCNQKGGV GKTTSTINLG ASLAEYGRRV LLVDLDPQGA LSAGLGVPHY ELEHTVHNLL VEPRVSIDDV VIKTRVSGMD LVPSNIDLSA AEIQLVNEVG REQSLARALY PVLDRYDYVL IDCQPSLGLL TVNGLACADG VIIPTECEYF SLRGLALLTD TVDKVHDRLN PKLSISGILV TRYDPRTVNA REVMARVVER FGDLVFDTVI TRTVRFPETS VAGEPITTWA PKSGGAIAYR SLAREVIDRF GA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acgacagcgc acacgacggc ctcttcgagc acagctcacc cgagctcggt ttgaccgggc ggccgccgcg cgacatcccc gaaccgaagg cccgcacagt gcatggcccg gccaaggtca tcgcgatgtg caaccagaag ggcggggtcg gtaagaccac gtcgaccatc aacctgggcg ccagcctggc cgagtacggc cgccgcgtcc tgctggtgga cctggacccc caaggcgcgc tgtcggcggg cctcggcgtg ccgcactacg aactcgagca cacggtgcac aacctgctgg tcgaaccgcg ggtgtccatc gacgacgtcg tcatcaagac ccgcgtcagc ggcatggatc tggtgcccag caacatcgac ctgtcggccg cggagatcca gctggtcaac gaggtgggcc gcgagcagtc cctggcccgt gccctgtatc cggtgctgga ccgctacgac tacgtgctga tcgactgcca gccgtcgctg ggcctgctga cagtcaacgg gctggcctgc gccgacggtg tgatcatccc caccgaatgc gagtacttct cgctgcgcgg gctggccctg ctgaccgaca ccgtggacaa ggtgcacgac cggctcaacc cgaagctgtc gatcagcggc atcctggtga cccgctacga cccgcgcacc gtcaacgccc gcgaggtcat ggctcgcgtc gtggagcggt tcggtgacct ggtgttcgac acggtgatca cccggaccgt gcggttcccg gagaccagcg tggccggcga gccgatcacc acctgggcgc ccaagtcggg tggcgccatc gcctatcgat cgctggcacg cgaagtcatc gaccggttcg gcgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyfoA.11416.a.B1.PS37980

PURIFICATION DATe:	7/19/2016
CONCENTRATION:	30mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACGACAGCGC ACACGACGGC CTCTTCGAGC ACAGCTCACC CGAGCTCGGT TTGACCGGGC GGCCGCCGCG CGACATCCCC GAACCGAAGG CCCGCACAGT GCATGGCCCG GCCAAGGTCA TCGCGATGTG CAACCAGAAG GGCGGGGTCG GTAAGACCAC GTCGACCATC AACCTGGGCG CCAGCCTGGC CGAGTACGGC CGCCGCGTCC TGCTGGTGGA CCTGGACCCC CAAGGCGCGC TGTCGGCGGG CCTCGGCGTG CCGCACTACG AACTCGAGCA CACGGTGCAC AACCTGCTGG TCGAACCGCG GGTGTCCATC GACGACGTCG TCATCAAGAC CCGCGTCAGC GGCATGGATC TGGTGCCCAG CAACATCGAC CTGTCGGCCG CGGAGATCCA GCTGGTCAAC GAGGTGGGCC GCGAGCAGTC CCTGGCCCGT GCCCTGTATC CGGTGCTGGA CCGCTACGAC TACGTGCTGA TCGACTGCCA GCCGTCGCTG GGCCTGCTGA CAGTCAACGG GCTGGCCTGC GCCGACGGTG TGATCATCCC CACCGAATGC GAGTACTTCT CGCTGCGCGG GCTGGCCCTG CTGACCGACA CCGTGGACAA GGTGCACGAC CGGCTCAACC CGAAGCTGTC GATCAGCGGC ATCCTGGTGA CCCGCTACGA CCCGCGCACC GTCAACGCCC GCGAGGTCAT GGCTCGCGTC GTGGAGCGGT TCGGTGACCT GGTGTTCGAC ACGGTGATCA CCCGGACCGT GCGGTTCCCG GAGACCAGCG TGGCCGGCGA GCCGATCACC ACCTGGGCGC CCAAGTCGGG TGGCGCCATC GCCTATCGAT CGCTGGCACG CGAAGTCATC GACCGGTTCG GCGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT