SSGCID: Target Page

MyleA.11416.b

Possible regulatory protein (Rv3918c ortholog)

CENTER ID:	MyleA.11416.b
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.11416.b.B1.GE37463	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2528
RefSeq:	YP_002503605.1
UniProt:	A0A0H3MR08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIDHLDSVVE IGLTGRPPRA IPEPRPRSSH GPAKVVAMCN QKGGVGKTTS TINLGAALTE FGRRVLLVDI DPQGALSAGL GVPHYELDRT IHNLMVEPLV SIDDVLIHTR VRYLDLVPSN IDLSAAEIQL VNEVGREQTL ARALHPVLDR YDYVLIDCQP SLGLLTVNGL ACAEGVVIPT ECEFFSLRGL ALLTDTVDKV RDRLNPKLEI SGILITRYDP RTVNAREVMA RVVERFGDLV FDTVITRTVR FPETSVAGEP ITTWAPKSGG ARAYRALACE FIDRFGA

NT Sequence

atgatcgacc acctcgatag cgttgtagag atcgggctga ccggacggcc gccacgggcc atcccagaac ccaggccgcg tagctcgcac ggtccggcca aggtcgtcgc gatgtgcaac cagaagggtg gcgtcgggaa aaccacgtcg acgatcaacc tgggtgccgc cctcaccgaa ttcggccgga gggtgctgct ggtggatata gacccgcagg gcgcactgtc ggcaggcctt ggcgtaccgc attacgagct agaccggaca atccacaacc taatggtgga accactggta tcgatcgacg acgtgctgat ccacacacgg gtcagatact tagatttggt acccagcaat atcgacctgt cggctgccga gatccagttg gttaacgagg tggggcgaga gcagaccttg gcgcgggcgt tgcacccggt actggaccgc tacgattatg tgctgattga ctgccagcct tcgctgggcc tactcaccgt taacggactg gcttgtgcag agggcgtagt tatcccgacg gaatgcgaat tcttctcgct gcgtgggctg gcgttgctta ccgacaccgt agacaaggtg cgtgatcggc tcaacccgaa gttggaaatc agcggcatcc tgattactcg atatgacccg cgcactgtca acgcacgcga ggttatggca cgtgtcgtag aacggttcgg cgacctggtc tttgacactg tgatcacccg tacggtccgg tttcctgaga ccagtgtcgc gggtgaaccc atcaccacgt gggcaccgaa atcgggcggc gccagggcct atcgcgcatt ggcctgtgaa ttcatcgacc gcttcggcgc gtga

Details for MyleA.11416.b.B1.GE37463

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI DHLDSVVEIG LTGRPPRAIP EPRPRSSHGP AKVVAMCNQK GGVGKTTSTI NLGAALTEFG RRVLLVDIDP QGALSAGLGV PHYELDRTIH NLMVEPLVSI DDVLIHTRVR YLDLVPSNID LSAAEIQLVN EVGREQTLAR ALHPVLDRYD YVLIDCQPSL GLLTVNGLAC AEGVVIPTEC EFFSLRGLAL LTDTVDKVRD RLNPKLEISG ILITRYDPRT VNAREVMARV VERFGDLVFD TVITRTVRFP ETSVAGEPIT TWAPKSGGAX AYRALACEFI DRXXX

Validated NT Sequence

tnnnttnnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgatcga ccacctcgat agcgttgtag agatcgggct gaccggacgg ccgccacggg ccatcccaga acccaggccg cgtagctcgc acggtccggc caaggtcgtc gcgatgtgca accagaaggg tggcgtcggg aaaaccacgt cgacgatcaa cctgggtgcc gccctcaccg aattcggccg gagggtgctg ctggtggata tagacccgca gggcgcactg tcggcaggcc ttggcgtacc gcattacgag ctagaccgga caatccacaa cctaatggtg gaaccactgg tatcgatcga cgacgtgctg atccacacac gggtcagata cttagatttg gtacccagca atatcgacct gtcggctgcc gagatccagt tggttaacga ggtggggcga gagcagacct tggcgcgggc gttgcacccg gtactggacc gctacgatta tgtgctgatt gactgccagc cttcgctggg cctactcacc gttaacggac tggcttgtgc agagggcgta gttatcccga cggaatgcga attcttctcg ctgcgtgggc tggcgttgct taccgacacc gtagacaagg tgcgtgatcg gctcaacccg aagttggaaa tcagcggcat cctgattact cgatatgacc cgcgcactgt caacgcacgc gaggttatgg cacgtgtcgt agaacggttc ggcgacctgg tctttgacac tgtgatcacc cgtacggtcc ggtttcctga gaccagtgtc gcgggtgaac ccatcaccac gtgggcaccg aaatcgggcg gcgccngggc ctatcgcgca ttggcctgtg aattcatcga ccgctnnnnn ncgtaagtga ntananagna ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntnann nnnnnnnnna nnnnnnntnn naggntnncn nnnnaataaa nanannnnnn nnnnnnancn ctngnnnnnn nnnnnngnnn nnngnnnagn cnaannnnnn nnnnnnnnnn nnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg accacctcga tagcgttgta gagatcgggc tgaccggacg gccgccacgg gccatcccag aacccaggcc gcgtagctcg cacggtccgg ccaaggtcgt cgcgatgtgc aaccagaagg gtggcgtcgg gaaaaccacg tcgacgatca acctgggtgc cgccctcacc gaattcggcc ggagggtgct gctggtggat atagacccgc agggcgcact gtcggcaggc cttggcgtac cgcattacga gctagaccgg acaatccaca acctaatggt ggaaccactg gtatcgatcg acgacgtgct gatccacaca cgggtcagat acttagattt ggtacccagc aatatcgacc tgtcggctgc cgagatccag ttggttaacg aggtggggcg agagcagacc ttggcgcggg cgttgcaccc ggtactggac cgctacgatt atgtgctgat tgactgccag ccttcgctgg gcctactcac cgttaacgga ctggcttgtg cagagggcgt agttatcccg acggaatgcg aattcttctc gctgcgtggg ctggcgttgc ttaccgacac cgtagacaag gtgcgtgatc ggctcaaccc gaagttggaa atcagcggca tcctgattac tcgatatgac ccgcgcactg tcaacgcacg cgaggttatg gcacgtgtcg tagaacggtt cggcgacctg gtctttgaca ctgtgatcac ccgtacggtc cggtttcctg agaccagtgt cgcgggtgaa cccatcacca cgtgggcacc gaaatcgggc ggcgccaggg cctatcgcgc attggcctgt gaattcatcg accgcttcgg cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat