SSGCID: Target Page

MythA.11416.b

Cobyrinic acid a,c-diamide synthase (Rv3918c ortholog)

CENTER ID:	MythA.11416.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.11416.b.B1.GE37650	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.3883
RefSeq:	WP_003927407.1
UniProt:	G7CLP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDDAVTAEV VGLTGRPPRP IPEPPPKSTH GPAKVIAMCN QKGGVGKTTS TINLGASLAE YGRRVLLVDL DPQGALSAGL GVPHYDLDHT VYNLLVEPRV SIDEVLVSTR VRNLDLVPSN IDLSAAEIQL VTEVGREQAL ARALHPVLDR YDYVLIDCQP SLGLLTVNGL ACSDGVIIPT ECEYFSLRGL ALLTDTVDKV RDRLNPKLEI SGILVTRYDP RTVNSREVMA RVVERFDDLV FDTVITRTVR FPETSVAGEP ITTWAPKSTG AEAYRSLARE VIHRFGA

NT Sequence

atgaccgacg acgcagtcac cgcagaagtc gtcggcctga ccggccggcc accacgaccg ataccggaac cgccgccgaa atccacccac ggaccggcca aggtcatcgc gatgtgcaac cagaagggcg gcgtcggcaa gaccacctcg acgatcaacc tcggcgccag cctggccgaa tacgggcgcc gggtgctgct ggtggacctc gatccgcagg gcgccctgtc ggccggcctc ggggtgccgc actacgacct cgaccacacc gtctacaacc tgctggtcga accccgggtg tcgatcgacg aggtgctggt ctccacccgg gtccgcaacc tggacctggt gcccagcaac atcgacctgt ccgccgccga gatccagctg gtcaccgagg tgggccgcga gcaggccctg gcccgcgccc tgcacccggt gctggaccgc tacgactacg tgctgatcga ctgccaaccc tcgctggggc tgctcaccgt gaacgggttg gcctgctccg acggggtcat catcccgacc gaatgcgaat acttctcgct gcgcggtctg gcgctgctga ccgacaccgt cgacaaggtc cgcgaccggc tcaacccgaa gctggagatc agcggcatcc tggtcacccg ctacgatccg cgcaccgtca actcccgcga ggtgatggcc cgcgtggtgg agcggttcga cgatctggtg ttcgacacgg tcatcacccg gaccgtgcga ttcccggaga ccagcgtcgc cggcgaaccg atcaccacct gggcgccgaa gtccaccggc gccgaggcct accggtcgct ggcgcgtgag gtgattcacc ggttcggcgc gtga

Details for MythA.11416.b.B1.GE37650

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DDAVTAEVVG LTGRPPRPIP EPPPKSTHGP AKVIAMCNQK GGVGKTTSTI NLGASLAEYG RRVLLVDLDP QGALSAGLGV PHYDLDHTVY NLLVEPRVSI DEVLVSTRVR NLDLVPSNID LSAAEIQLVT EVGREQALAR ALHPVLDRYD YVLIDCQPSL GLLTVNGLAC SDGVIIPTEC EYFSLRGLAL LTDTVDKVRD RLNPKLEISG ILVTRYDPRT VNSREVMARV VERFDDLVFD TVITRTVRFP ETSVAGEPIT TWAPKSTGAE AYRSLAREVI HRFGA

Validated NT Sequence

cnctagaatn nttttnttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgacc gacgacgcag tcaccgcaga agtcgtcggc ctgaccggcc ggccaccacg accgataccg gaaccgccgc cgaaatccac ccacggaccg gccaaggtca tcgcgatgtg caaccagaag ggcggcgtcg gcaagaccac ctcgacgatc aacctcggcg ccagcctggc cgaatacggg cgccgggtgc tgctggtgga cctcgatccg cagggcgccc tgtcggccgg cctcggggtg ccgcactacg acctcgacca caccgtctac aacctgctgg tcgaaccccg ggtgtcgatc gacgaggtgc tggtctccac ccgggtccgc aacctggacc tggtgcccag caacatcgac ctgtccgccg ccgagatcca gctggtcacc gaggtgggcc gcgagcaggc cctggcccgc gccctgcacc cggtgctgga ccgctacgac tacgtgctga tcgactgcca accctcgctg gggctgctca ccgtgaacgg gttggcctgc tccgacgggg tcatcatccc gaccgaatgc gaatacttct cgctgcgcgg tctggcgctg ctgaccgaca ccgtcgacaa ggtccgcgac cggctcaacc cgaagctgga gatcagcggc atcctggtca cccgctacga tccgcgcacc gtcaactccc gcgaggtgat ggcccgcgtg gtggagcggt tcgacgatct ggtgttcgac acggtcatca cccggaccgt gcgattcccg gagaccagcg tcgccggcga accgatcacc acctgggcgc cgaagtccac cggcgccgag gcctaccggt cgctggcgcg tgaggtgatt caccggttcg gcgcgtaagt gagtanatag gatccnntgt annanncccc ccnnanaagn nnntccgnca nnnnnnaaan nctgggtctg tccacccnat tcgangnccc ccncatnagt annncgnnnn nnncacaccg ataannttgt gncatctntn nt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acgacgcagt caccgcagaa gtcgtcggcc tgaccggccg gccaccacga ccgataccgg aaccgccgcc gaaatccacc cacggaccgg ccaaggtcat cgcgatgtgc aaccagaagg gcggcgtcgg caagaccacc tcgacgatca acctcggcgc cagcctggcc gaatacgggc gccgggtgct gctggtggac ctcgatccgc agggcgccct gtcggccggc ctcggggtgc cgcactacga cctcgaccac accgtctaca acctgctggt cgaaccccgg gtgtcgatcg acgaggtgct ggtctccacc cgggtccgca acctggacct ggtgcccagc aacatcgacc tgtccgccgc cgagatccag ctggtcaccg aggtgggccg cgagcaggcc ctggcccgcg ccctgcaccc ggtgctggac cgctacgact acgtgctgat cgactgccaa ccctcgctgg ggctgctcac cgtgaacggg ttggcctgct ccgacggggt catcatcccg accgaatgcg aatacttctc gctgcgcggt ctggcgctgc tgaccgacac cgtcgacaag gtccgcgacc ggctcaaccc gaagctggag atcagcggca tcctggtcac ccgctacgat ccgcgcaccg tcaactcccg cgaggtgatg gcccgcgtgg tggagcggtt cgacgatctg gtgttcgaca cggtcatcac ccggaccgtg cgattcccgg agaccagcgt cgccggcgaa ccgatcacca cctgggcgcc gaagtccacc ggcgccgagg cctaccggtc gctggcgcgt gaggtgattc accggttcgg cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat