SSGCID: Target Page

MysmA.11416.a

SpoOJ regulator protein (Rv3918c ortholog)

CENTER ID:	MysmA.11416.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.11416.a.B1.GE37507	full length		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.11416.a.B1.PS01981	full length		1	277

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3684
RefSeq:	YP_888042.1
UniProt:	A0QYQ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLTGRPPRE IPEPRPRETH GPAKVIAMCN QKGGVGKTTS TINLGASLAE YGRRVLLVDL DPQGALSAGL GVPHYELDNT VHNLLVEPRV SIDDVLIKTR VSGMDLVPSN IDLSAAEIQL VNEVGREQSL ARALYPVLDR YDYVLIDCQP SLGLLTINGL ACADGVVIPT ECEYFSLRGL ALLTDTVDKV HDRLNPKLSI SGILVTRYDP RTVNAREVMA RVVERFGDLV FDTVITRTVR FPETSVAGEP ITTWAPKSSG AAAYRSLARE VIHRFGV

NT Sequence

gtgggcctga cgggccggcc tccccgcgag atccccgagc cgcggccgcg tgagacgcac ggcccggcga aggtcatcgc gatgtgcaac cagaagggcg gcgtcggcaa gaccacgtcg accatcaacc tgggagcgag cctggccgag tacggcaggc gggtgctgct ggtcgacctc gacccgcagg gtgcactgtc ggcgggcctg ggtgttccgc actacgaact cgacaacacc gtgcacaacc tgctcgtgga gccgcgggtg tccatcgacg acgtgctgat caagacgcgg gtcagcggca tggacctggt gcccagcaac atcgacctgt cggctgccga gatccagctg gtcaacgagg tgggccgcga gcagtcactc gcgcgtgcgc tgtacccggt gctcgaccgc tacgactacg tgctgatcga ctgccagccg tcgctgggcc tgctcaccat caacggtctg gcgtgcgcgg acggcgtcgt catccccacc gaatgcgagt acttctcgct acgcggcctg gcgctgctca ccgacaccgt cgacaaggtg cacgaccggc tgaaccccaa gctgtcgatc agcggaatcc tggtgacccg ttacgacccg cgcacggtca acgcgcgtga ggtgatggcg cgggtggtcg aacggttcgg cgatctggtg ttcgacaccg tcatcacgcg caccgtccgg ttccccgaga ccagcgtcgc gggtgaaccg atcaccacat gggcgccgaa gtccagcggc gcggccgcct accggtcgct cgcgcgggaa gtcatccacc ggttcggcgt gtga

Details for MysmA.11416.a.B1.GE37507

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	31kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV GLTGRPPREI PEPRPRETHG PAKVIAMCNQ KGGVGKTTST INLGASLAEY GRRVLLVDLD PQGALSAGLG VPHYELDNTV HNLLVEPRVS IDDVLIKTRV SGMDLVPSNI DLSAAEIQLV NEVGREQSLA RALYPVLDRY DYVLIDCQPS LGLLTINGLA CADGVVIPTE CEYFSLRGLA LLTDTVDKVH DRLNPKLSIS GILVTRYDPR TVNAREVMAR VVERFGDLVF DTVITRTVRF PETSVAGEPI TTWAPKSSGA AAYRSLAREV IHRFGV

Validated NT Sequence

tcttactcac ttacacgccg aaccggtgga tgacttcccg cgcgagcgac cggtaggcgg ccgcgccgct ggacttcggc gcccatgtgg tgatcggttc acccgcgacg ctggtctcgg ggaaccggac ggtgcgcgtg atgacggtgt cgaacaccag atcgccgaac cgttcgacca cccgcgccat cacctcacgc gcgttgaccg tgcgcgggtc gtaacgggtc accaggattc cgctgatcga cagcttgggg ttcagccggt cgtgcacctt gtcgacggtg tcggtgagca gcgccaggcc gcgtagcgag aagtactcgc attcggtggg gatgacgacg ccgtccgcgc acgccagacc gttgatggtg agcaggccca gcgacggctg gcagtcgatc agcacgtagt cgtagcggtc gagcaccggg tacagcgcac gcgcgagtga ctgctcgcgg cccacctcgt tgaccagctg gatctcggca gccgacaggt cgatgttgct gggcaccagg tccatgccgc tgacccgcgt cttgatcagc acgtcgtcga tggacacccg cggctccacg agcaggttgt gcacggtgtt gtcgagttcg tagtgcggaa cacccaggcc cgccgacagt gcaccctgcg ggtcgaggtc gaccagcagc acccgcctgc cgtactcggc caggctcgct cccaggttga tggtcgacgt ggtcttgccg acgccgccct tctggttgca catcgcgatg accttcgccg ggccgtgcgt ctcacgcggc cgcggctcgg ggatctcgcg gggaggccgg cccgtcaggc ccaccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMG LTGRPPREIP EPRPRETHGP AKVIAMCNQK GGVGKTTSTI NLGASLAEYG RRVLLVDLDP QGALSAGLGV PHYELDNTVH NLLVEPRVSI DDVLIKTRVS GMDLVPSNID LSAAEIQLVN EVGREQSLAR ALYPVLDRYD YVLIDCQPSL GLLTINGLAC ADGVVIPTEC EYFSLRGLAL LTDTVDKVHD RLNPKLSISG ILVTRYDPRT VNAREVMARV VERFGDLVFD TVITRTVRFP ETSVAGEPIT TWAPKSSGAA AYRSLAREVI HRFGV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgggcc tgacgggccg gcctccccgc gagatccccg agccgcggcc gcgtgagacg cacggcccgg cgaaggtcat cgcgatgtgc aaccagaagg gcggcgtcgg caagaccacg tcgaccatca acctgggagc gagcctggcc gagtacggca ggcgggtgct gctggtcgac ctcgacccgc agggtgcact gtcggcgggc ctgggtgttc cgcactacga actcgacaac accgtgcaca acctgctcgt ggagccgcgg gtgtccatcg acgacgtgct gatcaagacg cgggtcagcg gcatggacct ggtgcccagc aacatcgacc tgtcggctgc cgagatccag ctggtcaacg aggtgggccg cgagcagtca ctcgcgcgtg cgctgtaccc ggtgctcgac cgctacgact acgtgctgat cgactgccag ccgtcgctgg gcctgctcac catcaacggt ctggcgtgcg cggacggcgt cgtcatcccc accgaatgcg agtacttctc gctacgcggc ctggcgctgc tcaccgacac cgtcgacaag gtgcacgacc ggctgaaccc caagctgtcg atcagcggaa tcctggtgac ccgttacgac ccgcgcacgg tcaacgcgcg tgaggtgatg gcgcgggtgg tcgaacggtt cggcgatctg gtgttcgaca ccgtcatcac gcgcaccgtc cggttccccg agaccagcgt cgcgggtgaa ccgatcacca catgggcgcc gaagtccagc ggcgcggccg cctaccggtc gctcgcgcgg gaagtcatcc accggttcgg cgtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MysmA.11416.a.B1.PS01981

PURIFICATION DATe:	1/29/2014
CONCENTRATION:	31.41mg/ml
OBSERVED MW:	29kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGGCC TGACGGGCCG GCCTCCCCGC GAGATCCCCG AGCCGCGGCC GCGTGAGACG CACGGCCCGG CGAAGGTCAT CGCGATGTGC AACCAGAAGG GCGGCGTCGG CAAGACCACG TCGACCATCA ACCTGGGAGC GAGCCTGGCC GAGTACGGCA GGCGGGTGCT GCTGGTCGAC CTCGACCCGC AGGGTGCACT GTCGGCGGGC CTGGGTGTTC CGCACTACGA ACTCGACAAC ACCGTGCACA ACCTGCTCGT GGAGCCGCGG GTGTCCATCG ACGACGTGCT GATCAAGACG CGGGTCAGCG GCATGGACCT GGTGCCCAGC AACATCGACC TGTCGGCTGC CGAGATCCAG CTGGTCAACG AGGTGGGCCG CGAGCAGTCA CTCGCGCGTG CGCTGTACCC GGTGCTCGAC CGCTACGACT ACGTGCTGAT CGACTGCCAG CCGTCGCTGG GCCTGCTCAC CATCAACGGT CTGGCGTGCG CGGACGGCGT CGTCATCCCC ACCGAATGCG AGTACTTCTC GCTACGCGGC CTGGCGCTGC TCACCGACAC CGTCGACAAG GTGCACGACC GGCTGAACCC CAAGCTGTCG ATCAGCGGAA TCCTGGTGAC CCGTTACGAC CCGCGCACGG TCAACGCGCG TGAGGTGATG GCGCGGGTGG TCGAACGGTT CGGCGATCTG GTGTTCGACA CCGTCATCAC GCGCACCGTC CGGTTCCCCG AGACCAGCGT CGCGGGTGAA CCGATCACCA CATGGGCGCC GAAGTCCAGC GGCGCGGCCG CCTACCGGTC GCTCGCGCGG GAAGTCATCC ACCGGTTCGG CGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT