MypaA.11416.a

Putative uncharacterized protein (Rv3918c ortholog)

CENTER ID: MypaA.11416.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.11416.a.B1.GE37478 full length 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1484
RefSeq: NP_960344.1
UniProt: Q740E1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAGRRVSRD EPVRLSDDVH LPNTPAMTER PDNGVELGLT GRPPRAIPEP QPRTSHGPAK VVAMCNQKGG VGKTTSTINL GAALAEYGRR VLLVDMDPQG ALSAGLGVPH YELEKTIHNV LVGPRVSIDD VLLQTRVKHM DLVPSNIDLS AAEIQLVNEV GREQTLGRAL HPVLDRYDYV LIDCQPSLGL LTVNGLACSD GVVIPTECEY FSLRGLALLT DTVDKVRDRL NPKLEISGIL LTRYDPRTVN AREVMARVVE RFGDLVFDTV ITRTVRFPET SVAGEPITTW APRSTGAIAY RALAREFIDR FGA
NT Sequence
ttgcctgcgg gtcgccgggt ttcgcgcgac gaacccgtta gactttccga cgatgttcac ctcccgaaca cccccgccat gaccgagcgg ccggacaacg gcgtcgagct cggcctgacc gggcggccgc cgcgggcgat cccggagcca cagccgcgca cctcgcacgg cccggccaaa gtcgtcgcca tgtgcaacca gaagggcggc gtcgggaaga ccacgtccac catcaacctg ggtgcggcgc tcgccgagta cggtcgccgg gtgctgctgg tggacatgga cccgcagggc gcgctgtcgg cgggcctggg tgtgccgcac tacgagctgg agaagaccat ccacaacgtg ctggtcgggc cgcgcgtgtc gatcgacgac gtgctgctgc agacccgggt caagcacatg gacctggtgc ccagcaacat cgacctgtcg gcggccgaga tccagctggt caacgaggtc ggccgcgagc agacgctggg ccgggcgctg cacccggtgc tggaccgcta cgactacgtg ctgatcgact gccagccgtc gctgggcctg ctcaccgtga acgggctggc ctgctccgac ggtgtggtga tccccaccga gtgcgagtac ttctcgctgc gcggcctggc gttgctcacc gacaccgtgg acaaggtgcg cgaccggctc aacccgaagc tggaaatcag cgggatcctg ctcacccggt acgacccgcg gaccgtcaac gcgcgcgagg tgatggcccg cgtcgtcgaa cgcttcggcg acctggtctt cgacaccgtc atcacccgca ccgtccggtt cccggagacc agcgtggcag gcgagcccat caccacctgg gccccgcgct ccaccggggc catcgcttac cgcgcgctgg cccgcgagtt catcgaccga ttcggcgcgt ga
Details for MypaA.11416.a.B1.GE37478
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML PAGRRVSRDE PVRLSDDVHL PNTPAMTERP DNGVELGLTG RPPRAIPEPQ PRTSHGPAKV VAMCNQKGGV GKTTSTINLG AALAEYGRRV LLVDMDPQGA LSAGLGVPHY ELEKTIHNVL VGPRVSIDDV LLQTRVKHMD LVPSNIDLSA AEIQLVNEVG REQTLGRALH PVLDRYDYVL IDCQPSLGLL TVNGLACSDG VVIPTECEYF SLRGLALLTD TVDKVRDRLN PKLEISGILL TRYDPRTVNA REVMARVVER FGDLVFDTVI TRTVRFPETS VAGEPITTWA PRSTGAIAYR ALAREFIDRF GA
Validated NT Sequence
tcttactcac ttacgcgccg aatcggtcga tgaactcgcg ggccagcgcg cggtaagcga tggccccggt ggagcgcggg gcccaggtgg tgatgggctc gcctgccacg ctggtctccg ggaaccggac ggtgcgggtg atgacggtgt cgaagaccag gtcgccgaag cgttcgacga cgcgggccat cacctcgcgc gcgttgacgg tccgcgggtc gtaccgggtg agcaggatcc cgctgatttc cagcttcggg ttgagccggt cgcgcacctt gtccacggtg tcggtgagca acgccaggcc gcgcagcgag aagtactcgc actcggtggg gatcaccaca ccgtcggagc aggccagccc gttcacggtg agcaggccca gcgacggctg gcagtcgatc agcacgtagt cgtagcggtc cagcaccggg tgcagcgccc ggcccagcgt ctgctcgcgg ccgacctcgt tgaccagctg gatctcggcc gccgacaggt cgatgttgct gggcaccagg tccatgtgct tgacccgggt ctgcagcagc acgtcgtcga tcgacacgcg cggcccgacc agcacgttgt ggatggtctt ctccagctcg tagtgcggca cacccaggcc cgccgacagc gcgccctgcg ggtccatgtc caccagcagc acccggcgac cgtactcggc gagcgccgca cccaggttga tggtggacgt ggtcttcccg acgccgccct tctggttgca catggcgacg actttggccg ggccgtgcga ggtgcgcggc tgtggctccg ggatcgcccg cggcggccgc ccggtcaggc cgagctcgac gccgttgtcc ggccgctcgg tcatggcggg ggtgttcggg aggtgaacat cgtcggaaag tctaacgggt tcgtcgcgcg aaacccggcg acccgcaggc aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMP AGRRVSRDEP VRLSDDVHLP NTPAMTERPD NGVELGLTGR PPRAIPEPQP RTSHGPAKVV AMCNQKGGVG KTTSTINLGA ALAEYGRRVL LVDMDPQGAL SAGLGVPHYE LEKTIHNVLV GPRVSIDDVL LQTRVKHMDL VPSNIDLSAA EIQLVNEVGR EQTLGRALHP VLDRYDYVLI DCQPSLGLLT VNGLACSDGV VIPTECEYFS LRGLALLTDT VDKVRDRLNP KLEISGILLT RYDPRTVNAR EVMARVVERF GDLVFDTVIT RTVRFPETSV AGEPITTWAP RSTGAIAYRA LAREFIDRFG A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcctg cgggtcgccg ggtttcgcgc gacgaacccg ttagactttc cgacgatgtt cacctcccga acacccccgc catgaccgag cggccggaca acggcgtcga gctcggcctg accgggcggc cgccgcgggc gatcccggag ccacagccgc gcacctcgca cggcccggcc aaagtcgtcg ccatgtgcaa ccagaagggc ggcgtcggga agaccacgtc caccatcaac ctgggtgcgg cgctcgccga gtacggtcgc cgggtgctgc tggtggacat ggacccgcag ggcgcgctgt cggcgggcct gggtgtgccg cactacgagc tggagaagac catccacaac gtgctggtcg ggccgcgcgt gtcgatcgac gacgtgctgc tgcagacccg ggtcaagcac atggacctgg tgcccagcaa catcgacctg tcggcggccg agatccagct ggtcaacgag gtcggccgcg agcagacgct gggccgggcg ctgcacccgg tgctggaccg ctacgactac gtgctgatcg actgccagcc gtcgctgggc ctgctcaccg tgaacgggct ggcctgctcc gacggtgtgg tgatccccac cgagtgcgag tacttctcgc tgcgcggcct ggcgttgctc accgacaccg tggacaaggt gcgcgaccgg ctcaacccga agctggaaat cagcgggatc ctgctcaccc ggtacgaccc gcggaccgtc aacgcgcgcg aggtgatggc ccgcgtcgtc gaacgcttcg gcgacctggt cttcgacacc gtcatcaccc gcaccgtccg gttcccggag accagcgtgg caggcgagcc catcaccacc tgggccccgc gctccaccgg ggccatcgct taccgcgcgc tggcccgcga gttcatcgac cgattcggcg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat