MyabA.00760.a

Putative uncharacterized protein (Rv3918c ortholog)

CENTER ID: MyabA.00760.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00760.a.B1.GE37295 full length 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00760.a.B1.PW37411 full length 1 293

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2410
RefSeq: YP_001703102.1
UniProt: B1MB27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDGSLDSDF PADDGDLDLT GRPPKDIPEP KPLTSHGPAK VIAMCNQKGG VGKTTSTINL GAALAGYGRR VLLVDLDPQG ALSAGLGIAH HELETTVHNL LVEPRVSVDD VLMRTRVEGL DLIPSNIDLS AAEIQLVNEV GREHSLARAL HPVLDRYDYV LIDCQPSLGL LTVNALACSE GVVIPMECAF FSLRGLALLT DTVAKVRDRL NPKLAVSGIV ITMFDARTLH AREVMARVIE VFGDQVFHTV ITRTVRFPET SVAGEPITTW APKSSGAQAY ISLAREVIDR FET
NT Sequence
gtgacggacg gcagtctcga ctctgacttc cccgccgacg acggcgacct tgatctcacc ggccgccccc cgaaagacat ccccgagccc aagccgctga ccagtcacgg gccggccaag gtgatcgcga tgtgcaacca gaagggcggc gtggggaaga ccacgtcgac catcaatctg ggcgccgcgc tggccggata cggccgccgg gtgctgctcg tcgacctcga tccacaaggt gcgctatcgg ccggtctggg catcgcccac cacgagctgg aaaccactgt gcacaacctg ctggtggagc ccagggtgtc ggtggacgac gtgctgatgc gcacgcgcgt cgagggactg gacctgatcc cgagcaatat cgacctctcc gccgccgaga ttcagctggt caacgaggtg gggcgcgagc attcactggc gcgggcgctg caccctgtgc tcgatcgcta cgactacgtg ctcatcgact gccagccgtc gctgggactg ttgacggtga acgcgctggc ctgctccgag ggcgtcgtca tcccgatgga atgtgcgttc ttctcgctgc gcgggctggc cctgctcacc gacaccgtgg ccaaggtgcg cgaccggctc aacccgaaac tggcggtgtc cggaatcgtc atcaccatgt tcgatgcccg gaccctgcac gcgcgtgagg tgatggcccg ggtcatcgag gtgttcggcg accaggtctt ccacaccgtc atcactcgca ccgtccgctt cccggagacc agcgtggcgg gggagccgat caccacgtgg gcaccgaagt cttcgggtgc ccaggcgtac atctcgttgg ctcgcgaggt aatcgaccgg ttcgaaacgt ga
Details for MyabA.00760.a.B1.GE37295
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TDGSLDSDFP ADDGDLDLTG RPPKDIPEPK PLTSHGPAKV IAMCNQKGGV GKTTSTINLG AALAGYGRRV LLVDLDPQGA LSAGLGIAHH ELETTVHNLL VEPRVSVDDV LMRTRVEGLD LIPSNIDLSA AEIQLVNEVG REHSLARALH PVLDRYDYVL IDCQPSLGLL TVNALACSEG VVIPMECAFF SLRGLALLTD TVAKVRDRLN PKLAVSGIVI TMFDARTLHA REVMARVIEV FGDQVFHTVI TRTVRFPETS VAGEPITTWA PKSSGAQAYI SLAREVIDRF ET
Validated NT Sequence
tactcactta cgtttcgaac cggtcgatta cctcgcgagc caacgagatg tacgcctggg cacccgaaga cttcggtgcc cacgtggtga tcggctcccc cgccacgctg gtctccggga agcggacggt gcgagtgatg acggtgtgga agacctggtc gccgaacacc tcgatgaccc gggccatcac ctcacgcgcg tgcagggtcc gggcatcgaa catggtgatg acgattccgg acaccgccag tttcgggttg agccggtcgc gcaccttggc cacggtgtcg gtgagcaggg ccagcccgcg cagcgagaag aacgcacatt ccatcgggat gacgacgccc tcggagcagg ccagcgcgtt caccgtcaac agtcccagcg acggctggca gtcgatgagc acgtagtcgt agcgatcgag cacagggtgc agcgcccgcg ccagtgaatg ctcgcgcccc acctcgttga ccagctgaat ctcggcggcg gagaggtcga tattgctcgg gatcaggtcc agtccctcga cgcgcgtgcg catcagcacg tcgtccaccg acaccctggg ctccaccagc aggttgtgca cagtggtttc cagctcgtgg tgggcgatgc ccagaccggc cgatagcgca ccttgtggat cgaggtcgac gagcagcacc cggcggccgt atccggccag cgcggcgccc agattgatgg tcgacgtggt cttccccacg ccgcccttct ggttgcacat cgcgatcacc ttggccggcc cgtgactggt cagcggcttg ggctcgggga tgtctttcgg ggggcggccg gtgagatcaa ggtcgccgtc gtcggcgggg aagtcagagt cgagactgcc gtccgtcacc atatggtggt ggtggtggtg agccatggta tntctcnttc ttaa
Expected Protein Sequence
MAHHHHHHMT DGSLDSDFPA DDGDLDLTGR PPKDIPEPKP LTSHGPAKVI AMCNQKGGVG KTTSTINLGA ALAGYGRRVL LVDLDPQGAL SAGLGIAHHE LETTVHNLLV EPRVSVDDVL MRTRVEGLDL IPSNIDLSAA EIQLVNEVGR EHSLARALHP VLDRYDYVLI DCQPSLGLLT VNALACSEGV VIPMECAFFS LRGLALLTDT VAKVRDRLNP KLAVSGIVIT MFDARTLHAR EVMARVIEVF GDQVFHTVIT RTVRFPETSV AGEPITTWAP KSSGAQAYIS LAREVIDRFE T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgg acggcagtct cgactctgac ttccccgccg acgacggcga ccttgatctc accggccgcc ccccgaaaga catccccgag cccaagccgc tgaccagtca cgggccggcc aaggtgatcg cgatgtgcaa ccagaagggc ggcgtgggga agaccacgtc gaccatcaat ctgggcgccg cgctggccgg atacggccgc cgggtgctgc tcgtcgacct cgatccacaa ggtgcgctat cggccggtct gggcatcgcc caccacgagc tggaaaccac tgtgcacaac ctgctggtgg agcccagggt gtcggtggac gacgtgctga tgcgcacgcg cgtcgaggga ctggacctga tcccgagcaa tatcgacctc tccgccgccg agattcagct ggtcaacgag gtggggcgcg agcattcact ggcgcgggcg ctgcaccctg tgctcgatcg ctacgactac gtgctcatcg actgccagcc gtcgctggga ctgttgacgg tgaacgcgct ggcctgctcc gagggcgtcg tcatcccgat ggaatgtgcg ttcttctcgc tgcgcgggct ggccctgctc accgacaccg tggccaaggt gcgcgaccgg ctcaacccga aactggcggt gtccggaatc gtcatcacca tgttcgatgc ccggaccctg cacgcgcgtg aggtgatggc ccgggtcatc gaggtgttcg gcgaccaggt cttccacacc gtcatcactc gcaccgtccg cttcccggag accagcgtgg cgggggagcc gatcaccacg tgggcaccga agtcttcggg tgcccaggcg tacatctcgt tggctcgcga ggtaatcgac cggttcgaaa cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MyabA.00760.a.B1.PW37411
PURIFICATION DATe: 12/2/2013
CONCENTRATION: 38.1mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TDGSLDSDFP ADDGDLDLTG RPPKDIPEPK PLTSHGPAKV IAMCNQKGGV GKTTSTINLG AALAGYGRRV LLVDLDPQGA LSAGLGIAHH ELETTVHNLL VEPRVSVDDV LMRTRVEGLD LIPSNIDLSA AEIQLVNEVG REHSLARALH PVLDRYDYVL IDCQPSLGLL TVNALACSEG VVIPMECAFF SLRGLALLTD TVAKVRDRLN PKLAVSGIVI TMFDARTLHA REVMARVIEV FGDQVFHTVI TRTVRFPETS VAGEPITTWA PKSSGAQAYI SLAREVIDRF ET
Validated NT Sequence
tactcactta cgtttcgaac cggtcgatta cctcgcgagc caacgagatg tacgcctggg cacccgaaga cttcggtgcc cacgtggtga tcggctcccc cgccacgctg gtctccggga agcggacggt gcgagtgatg acggtgtgga agacctggtc gccgaacacc tcgatgaccc gggccatcac ctcacgcgcg tgcagggtcc gggcatcgaa catggtgatg acgattccgg acaccgccag tttcgggttg agccggtcgc gcaccttggc cacggtgtcg gtgagcaggg ccagcccgcg cagcgagaag aacgcacatt ccatcgggat gacgacgccc tcggagcagg ccagcgcgtt caccgtcaac agtcccagcg acggctggca gtcgatgagc acgtagtcgt agcgatcgag cacagggtgc agcgcccgcg ccagtgaatg ctcgcgcccc acctcgttga ccagctgaat ctcggcggcg gagaggtcga tattgctcgg gatcaggtcc agtccctcga cgcgcgtgcg catcagcacg tcgtccaccg acaccctggg ctccaccagc aggttgtgca cagtggtttc cagctcgtgg tgggcgatgc ccagaccggc cgatagcgca ccttgtggat cgaggtcgac gagcagcacc cggcggccgt atccggccag cgcggcgccc agattgatgg tcgacgtggt cttccccacg ccgcccttct ggttgcacat cgcgatcacc ttggccggcc cgtgactggt cagcggcttg ggctcgggga tgtctttcgg ggggcggccg gtgagatcaa ggtcgccgtc gtcggcgggg aagtcagagt cgagactgcc gtccgtcacc atatggtggt ggtggtggtg agccatggta tntctcnttc ttaa
Expressed Protein Sequence
MAHHHHHHMT DGSLDSDFPA DDGDLDLTGR PPKDIPEPKP LTSHGPAKVI AMCNQKGGVG KTTSTINLGA ALAGYGRRVL LVDLDPQGAL SAGLGIAHHE LETTVHNLLV EPRVSVDDVL MRTRVEGLDL IPSNIDLSAA EIQLVNEVGR EHSLARALHP VLDRYDYVLI DCQPSLGLLT VNALACSEGV VIPMECAFFS LRGLALLTDT VAKVRDRLNP KLAVSGIVIT MFDARTLHAR EVMARVIEVF GDQVFHTVIT RTVRFPETSV AGEPITTWAP KSSGAQAYIS LAREVIDRFE T
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACGG ACGGCAGTCT CGACTCTGAC TTCCCCGCCG ACGACGGCGA CCTTGATCTC ACCGGCCGCC CCCCGAAAGA CATCCCCGAG CCCAAGCCGC TGACCAGTCA CGGGCCGGCC AAGGTGATCG CGATGTGCAA CCAGAAGGGC GGCGTGGGGA AGACCACGTC GACCATCAAT CTGGGCGCCG CGCTGGCCGG ATACGGCCGC CGGGTGCTGC TCGTCGACCT CGATCCACAA GGTGCGCTAT CGGCCGGTCT GGGCATCGCC CACCACGAGC TGGAAACCAC TGTGCACAAC CTGCTGGTGG AGCCCAGGGT GTCGGTGGAC GACGTGCTGA TGCGCACGCG CGTCGAGGGA CTGGACCTGA TCCCGAGCAA TATCGACCTC TCCGCCGCCG AGATTCAGCT GGTCAACGAG GTGGGGCGCG AGCATTCACT GGCGCGGGCG CTGCACCCTG TGCTCGATCG CTACGACTAC GTGCTCATCG ACTGCCAGCC GTCGCTGGGA CTGTTGACGG TGAACGCGCT GGCCTGCTCC GAGGGCGTCG TCATCCCGAT GGAATGTGCG TTCTTCTCGC TGCGCGGGCT GGCCCTGCTC ACCGACACCG TGGCCAAGGT GCGCGACCGG CTCAACCCGA AACTGGCGGT GTCCGGAATC GTCATCACCA TGTTCGATGC CCGGACCCTG CACGCGCGTG AGGTGATGGC CCGGGTCATC GAGGTGTTCG GCGACCAGGT CTTCCACACC GTCATCACTC GCACCGTCCG CTTCCCGGAG ACCAGCGTGG CGGGGGAGCC GATCACCACG TGGGCACCGA AGTCTTCGGG TGCCCAGGCG TACATCTCGT TGGCTCGCGA GGTAATCGAC CGGTTCGAAA CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT