MytuD.00458.a

PROBABLE ACYL-CoA THIOESTERASE II TESB2 (TEII)

CENTER ID: MytuD.00458.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00458.a.A1.GE25843 Full length( MytuD.00458.a ) 1 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00458.a.A1.PS00295 Full length( MytuD.00458.a ) 1 281

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2915
RefSeq: NP_217121.1
TubercuList: Rv2605c
MTB Network Portal: Rv2605c
UniProt: I6X4S7

Orthologues for Rv2605c

Species % Identity % Coverage Clones Proteins Structures
avium 87 99 1 0 0
marinum 85 99 1 1 1
ulcerans 85 99 1 2 0
smegmatis 80 98 0 0 0
thermoresistibile 78 98 1 1 0
abscessus 66 97 1 2 0
smegmatis 37 90 1 1 0
avium 34 97 1 1 1
smegmatis 32 96 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIEEILDLE QLEVNIYRGS VFSPESGFLQ RTFGGHVAGQ SLVSAVRTVD PRYMVHSLHG YFLRPGDAKE RTVFLVERIR DGGSFCTRRV NAVQHGETIF SMAASFQTEQ EGITHQDVMP AAPPPDGLPG LNSIKVFDDA GFRQFDEWDV CIVPRERLRL LPGKASQQQV WLRHRDPLPD DPVLHICALA YMSDLTLLGS AQVNHLDVRD QLQVASLDHA MWFMRPFRAD EWLLYDQSSP SASGGRALTR GEIFTRSGEM VAAVMQEGLT RHRRGHRSVG Q
NT Sequence
GTGTCGATCG AAGAGATCCT TGATCTTGAG CAACTTGAGG TCAACATCTA CCGAGGAAGC GTGTTCAGCC CCGAATCGGG CTTCCTGCAA CGCACTTTCG GTGGTCATGT AGCCGGCCAG TCGCTGGTGT CGGCGGTGCG CACCGTCGAC CCGCGGTACA TGGTGCACTC GCTGCACGGC TATTTTCTTC GGCCTGGAGA TGCCAAGGAG CGCACGGTTT TTCTGGTCGA ACGCATCCGC GACGGTGGAT CGTTCTGCAC CAGGCGGGTC AACGCAGTCC AGCACGGGGA AACCATATTC AGCATGGCGG CGTCGTTCCA GACCGAACAG GAGGGCATTA CCCACCAAGA CGTTATGCCA GCGGCGCCGC CGCCCGACGG CCTGCCGGGG TTGAACTCGA TCAAGGTTTT CGATGATGCC GGGTTCCGGC AGTTCGACGA GTGGGACGTG TGCATCGTGC CCCGGGAGCG CCTGCGGCTT TTGCCGGGCA AGGCTTCCCA GCAGCAGGTG TGGCTTCGCC ACCGTGACCC GTTGCCCGAC GACCCGGTGT TGCACATCTG TGCACTCGCC TACATGAGCG ACCTCACGCT GCTGGGATCG GCACAAGTCA ACCACCTCGA TGTGCGCGAC CAGCTGCAGG TGGCATCGCT GGACCATGCG ATGTGGTTCA TGCGACCCTT CCGCGCCGAC GAGTGGTTGC TGTACGACCA GTCGTCGCCG TCGGCCAGCG GCGGTCGTGC GCTGACTCGG GGCGAGATTT TCACACGGTC CGGGGAGATG GTCGCGGCGG TCATGCAGGA GGGGCTGACC CGCCACCGGC GCGGTCACCG GTCGGTGGGG CAG
Details for MytuD.00458.a.A1.GE25843
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYMVHSL HGYFLRPGDA KERTVFLVER IRDGGSFCTR RVNAVQHGET IFSMAASFQT EQEGITHQDV MPAAPPPDGL PGLNSIKVFD DAGFRQFDEW DVCIVPRERL RLLPGKASQQ QVWLRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVNHLDV RDQLQVASLD HAMWFMRPFR ADEWLLYDQS SPSASGGRAL TRGEIFTRSG EMVAAVMQEG LTRHRRGHRS VGQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt cgatcgaaga gatccttgat cttgagcaac ttgaggtcaa catctaccga ggaagcgtgt tcagccccga atcgggcttc ctgcaacgca ctttcggtgg tcatgtagcc ggccagtcgc tggtgtcggc ggtgcgcacc gtcgacccgc ggtacatggt gcactcgctg cacggctatt ttcttcggcc tggagatgcc aaggagcgca cggtttttct ggtcgaacgc atccgcgacg gtggatcgtt ctgcaccagg cgggtcaacg cagtccagca cggggaaacc atattcagca tggcggcgtc gttccagacc gaacaggagg gcattaccca ccaagacgtt atgccagcgg cgccgccgcc cgacggcctg ccggggttga actcgatcaa ggttttcgat gatgccgggt tccggcagtt cgacgagtgg gacgtgtgca tcgtgccccg ggagcgcctg cggcttttgc cgggcaaggc ttcccagcag caggtgtggc ttcgccaccg tgacccgttg cccgacgacc cggtgttgca catctgtgca ctcgcctaca tgagcgacct cacgctgctg ggatcggcac aagtcaacca cctcgatgtg cgcgaccagc tgcaggtggc atcgctggac catgcgatgt ggttcatgcg acccttccgc gccgacgagt ggttgctgta cgaccagtcg tcgccgtcgg ccagcggcgg tcgtgcgctg actcggggcg agattttcac acggtccggg gagatggtcg cggcggtcat gcaggagggg ctgacccgcc accggcgcgg tcaccggtcg gtggggcagt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYMVHSL HGYFLRPGDA KERTVFLVER IRDGGSFCTR RVNAVQHGET IFSMAASFQT EQEGITHQDV MPAAPPPDGL PGLNSIKVFD DAGFRQFDEW DVCIVPRERL RLLPGKASQQ QVWLRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVNHLDV RDQLQVASLD HAMWFMRPFR ADEWLLYDQS SPSASGGRAL TRGEIFTRSG EMVAAVMQEG LTRHRRGHRS VGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcgatc gaagagatcc ttgatcttga gcaacttgag gtcaacatct accgaggaag cgtgttcagc cccgaatcgg gcttcctgca acgcactttc ggtggtcatg tagccggcca gtcgctggtg tcggcggtgc gcaccgtcga cccgcggtac atggtgcact cgctgcacgg ctattttctt cggcctggag atgccaagga gcgcacggtt tttctggtcg aacgcatccg cgacggtgga tcgttctgca ccaggcgggt caacgcagtc cagcacgggg aaaccatatt cagcatggcg gcgtcgttcc agaccgaaca ggagggcatt acccaccaag acgttatgcc agcggcgccg ccgcccgacg gcctgccggg gttgaactcg atcaaggttt tcgatgatgc cgggttccgg cagttcgacg agtgggacgt gtgcatcgtg ccccgggagc gcctgcggct tttgccgggc aaggcttccc agcagcaggt gtggcttcgc caccgtgacc cgttgcccga cgacccggtg ttgcacatct gtgcactcgc ctacatgagc gacctcacgc tgctgggatc ggcacaagtc aaccacctcg atgtgcgcga ccagctgcag gtggcatcgc tggaccatgc gatgtggttc atgcgaccct tccgcgccga cgagtggttg ctgtacgacc agtcgtcgcc gtcggccagc ggcggtcgtg cgctgactcg gggcgagatt ttcacacggt ccggggagat ggtcgcggcg gtcatgcagg aggggctgac ccgccaccgg cgcggtcacc ggtcggtggg gcagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.00458.a.A1.PS00295
PURIFICATION DATe: 4/15/2009
CONCENTRATION: 5.86mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYMVHSL HGYFLRPGDA KERTVFLVER IRDGGSFCTR RVNAVQHGET IFSMAASFQT EQEGITHQDV MPAAPPPDGL PGLNSIKVFD DAGFRQFDEW DVCIVPRERL RLLPGKASQQ QVWLRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVNHLDV RDQLQVASLD HAMWFMRPFR ADEWLLYDQS SPSASGGRAL TRGEIFTRSG EMVAAVMQEG LTRHRRGHRS VGQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt cgatcgaaga gatccttgat cttgagcaac ttgaggtcaa catctaccga ggaagcgtgt tcagccccga atcgggcttc ctgcaacgca ctttcggtgg tcatgtagcc ggccagtcgc tggtgtcggc ggtgcgcacc gtcgacccgc ggtacatggt gcactcgctg cacggctatt ttcttcggcc tggagatgcc aaggagcgca cggtttttct ggtcgaacgc atccgcgacg gtggatcgtt ctgcaccagg cgggtcaacg cagtccagca cggggaaacc atattcagca tggcggcgtc gttccagacc gaacaggagg gcattaccca ccaagacgtt atgccagcgg cgccgccgcc cgacggcctg ccggggttga actcgatcaa ggttttcgat gatgccgggt tccggcagtt cgacgagtgg gacgtgtgca tcgtgccccg ggagcgcctg cggcttttgc cgggcaaggc ttcccagcag caggtgtggc ttcgccaccg tgacccgttg cccgacgacc cggtgttgca catctgtgca ctcgcctaca tgagcgacct cacgctgctg ggatcggcac aagtcaacca cctcgatgtg cgcgaccagc tgcaggtggc atcgctggac catgcgatgt ggttcatgcg acccttccgc gccgacgagt ggttgctgta cgaccagtcg tcgccgtcgg ccagcggcgg tcgtgcgctg actcggggcg agattttcac acggtccggg gagatggtcg cggcggtcat gcaggagggg ctgacccgcc accggcgcgg tcaccggtcg gtggggcagt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYMVHSL HGYFLRPGDA KERTVFLVER IRDGGSFCTR RVNAVQHGET IFSMAASFQT EQEGITHQDV MPAAPPPDGL PGLNSIKVFD DAGFRQFDEW DVCIVPRERL RLLPGKASQQ QVWLRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVNHLDV RDQLQVASLD HAMWFMRPFR ADEWLLYDQS SPSASGGRAL TRGEIFTRSG EMVAAVMQEG LTRHRRGHRS VGQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGATC GAAGAGATCC TTGATCTTGA GCAACTTGAG GTCAACATCT ACCGAGGAAG CGTGTTCAGC CCCGAATCGG GCTTCCTGCA ACGCACTTTC GGTGGTCATG TAGCCGGCCA GTCGCTGGTG TCGGCGGTGC GCACCGTCGA CCCGCGGTAC ATGGTGCACT CGCTGCACGG CTATTTTCTT CGGCCTGGAG ATGCCAAGGA GCGCACGGTT TTTCTGGTCG AACGCATCCG CGACGGTGGA TCGTTCTGCA CCAGGCGGGT CAACGCAGTC CAGCACGGGG AAACCATATT CAGCATGGCG GCGTCGTTCC AGACCGAACA GGAGGGCATT ACCCACCAAG ACGTTATGCC AGCGGCGCCG CCGCCCGACG GCCTGCCGGG GTTGAACTCG ATCAAGGTTT TCGATGATGC CGGGTTCCGG CAGTTCGACG AGTGGGACGT GTGCATCGTG CCCCGGGAGC GCCTGCGGCT TTTGCCGGGC AAGGCTTCCC AGCAGCAGGT GTGGCTTCGC CACCGTGACC CGTTGCCCGA CGACCCGGTG TTGCACATCT GTGCACTCGC CTACATGAGC GACCTCACGC TGCTGGGATC GGCACAAGTC AACCACCTCG ATGTGCGCGA CCAGCTGCAG GTGGCATCGC TGGACCATGC GATGTGGTTC ATGCGACCCT TCCGCGCCGA CGAGTGGTTG CTGTACGACC AGTCGTCGCC GTCGGCCAGC GGCGGTCGTG CGCTGACTCG GGGCGAGATT TTCACACGGT CCGGGGAGAT GGTCGCGGCG GTCATGCAGG AGGGGCTGAC CCGCCACCGG CGCGGTCACC GGTCGGTGGG GCAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA