MymaA.00458.a
Acyl-CoA thioesterase
| CENTER ID: | MymaA.00458.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00458.a.A1.GE30028 | Full length( MymaA.00458.a ) | 1 | 281 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00458.a.A1.PS00828 | Full length( MymaA.00458.a ) | 1 | 281 |
Structures
3U0A
DEPOSITED: 9/28/2011
DETERMINATION: XRay
CLONE: MymaA.00458.a.A1.GE30028
PROTEIN: MymaA.00458.a.A1.PS00828
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216594.6.peg.2238 |
| RefSeq: | YP_001850400.1 |
| UniProt: | B2HN47 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIEEILDLE QLEVNIYRGS VFSPESGFLQ RTFGGHVAGQ SLVSAVRTVD PRYQVHSLHG YFLRSGDAQE PTVFLVERTR DGGSFVTRRV NAVQHGEVIF SMGASFQTAQ NGISHQDAMP AAPPPDDLPG LRSVRVFDDA GFRQFEEWDV RIVPRDLLAP LPGKASQQQV WFRHRDPLPD DPVLHICALA YMSDLTLLGS AQVTHLAERE HLQVASLDHA MWFMRGFRAD EWLLYDQSSP SAGGGRALTH GKIFTQGGEL VAAVMQEGLT RYPSGYQPGE K
NT Sequence
GTGGCAATCG AGGAGATTCT TGATCTCGAG CAACTCGAGG TCAACATCTA TCGCGGTAGT GTCTTCAGCC CGGAATCAGG CTTTCTGCAG CGCACATTCG GCGGCCATGT GGCCGGGCAG TCACTGGTGT CGGCGGTGCG CACGGTCGAC CCGCGCTATC AGGTCCATTC GTTGCACGGG TACTTTCTGC GCTCCGGGGA CGCCCAGGAG CCCACGGTTT TTCTGGTCGA GCGCACTCGC GATGGCGGAT CGTTTGTGAC CAGGCGGGTC AACGCCGTTC AGCACGGCGA GGTCATCTTC AGCATGGGGG CTTCGTTTCA GACCGCGCAG AACGGCATCA GCCACCAGGA CGCGATGCCG GCGGCGCCAC CGCCCGACGA TCTACCCGGA TTGAGGTCGG TGCGGGTCTT CGACGACGCC GGATTCCGGC AGTTCGAGGA GTGGGATGTG CGCATCGTGC CGCGGGACCT GCTCGCGCCG CTACCGGGTA AGGCATCCCA GCAGCAGGTG TGGTTTCGCC ATCGCGACCC GCTGCCCGAT GACCCGGTGC TGCACATCTG TGCCTTGGCC TACATGAGCG ATCTGACTCT GCTGGGTTCG GCGCAGGTCA CCCACCTGGC CGAGCGTGAG CATCTGCAGG TGGCGTCCCT GGACCACGCG ATGTGGTTCA TGCGGGGGTT TCGGGCCGAC GAGTGGCTGC TCTACGACCA GTCATCGCCG TCGGCAGGCG GGGGTCGCGC ACTTACGCAC GGCAAGATCT TCACCCAGGG AGGCGAGTTG GTGGCCGCGG TCATGCAGGA AGGGCTGACC CGCTACCCAA GCGGATATCA GCCGGGCGAA AAG
Details for MymaA.00458.a.A1.GE30028
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GE
Validated NT Sequence
ttattattcg cccggctgat atccgcttgg gtagcgggtc agcccttcct gcatgaccgc ggccaccaac tcgcctccct gggtgaagat cttgccgtgc gtaagtgcgc gacccccgcc tgccgacggc gatgactggt cgtagagcag ccactcgtcg gcccgaaacc cccgcatgaa ccacatcgcg tggtccaggg acgccacctg cagatgctca cgctcggcca ggtgggtgac ctgcgccgaa cccagcagag tcagatcgct catgtaggcc aaggcacaga tgtgcagcac cgggtcatcg ggcagcgggt cgcgatggcg aaaccacacc tgctgctggg atgccttacc cggtagcggc gcgagcaggt cccgcggcac gatgcgcaca tcccactcct cgaactgccg gaatccggcg tcgtcgaaga cccgcaccga cctcaatccg ggtagatcgt cgggcggtgg cgccgccggc atcgcgtcct ggtggctgat gccgttctgc gcggtctgaa acgaagcccc catgctgaag atgacctcgc cgtgctgaac ggcgttgacc cgcctggtca caaacgatcc gccatcgcga gtgcgctcga ccagaaaaac cgtgggctcc tgggcgtccc cggagcgcag aaagtacccg tgcaacgaat ggacctgata gcgcgggtcg accgtgcgca ccgccgacac cagtgactgc ccggccacat ggccgccgaa tgtgcgctgc agaaagcctg attccgggct gaagacacta ccgcgataga tgttgacctc gagttgctcg agatcaagaa tctcctcgat tgccaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcaatc gaggagattc ttgatctcga gcaactcgag gtcaacatct atcgcggtag tgtcttcagc ccggaatcag gctttctgca gcgcacattc ggcggccatg tggccgggca gtcactggtg tcggcggtgc gcacggtcga cccgcgctat caggtccatt cgttgcacgg gtactttctg cgctccgggg acgcccagga gcccacggtt tttctggtcg agcgcactcg cgatggcgga tcgtttgtga ccaggcgggt caacgccgtt cagcacggcg aggtcatctt cagcatgggg gcttcgtttc agaccgcgca gaacggcatc agccaccagg acgcgatgcc ggcggcgcca ccgcccgacg atctacccgg attgaggtcg gtgcgggtct tcgacgacgc cggattccgg cagttcgagg agtgggatgt gcgcatcgtg ccgcgggacc tgctcgcgcc gctaccgggt aaggcatccc agcagcaggt gtggtttcgc catcgcgacc cgctgcccga tgacccggtg ctgcacatct gtgccttggc ctacatgagc gatctgactc tgctgggttc ggcgcaggtc acccacctgg ccgagcgtga gcatctgcag gtggcgtccc tggaccacgc gatgtggttc atgcgggggt ttcgggccga cgagtggctg ctctacgacc agtcatcgcc gtcggcaggc gggggtcgcg cacttacgca cggcaagatc ttcacccagg gaggcgagtt ggtggccgcg gtcatgcagg aagggctgac ccgctaccca agcggatatc agccgggcga aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.00458.a.A1.PS00828
| PURIFICATION DATe: | 10/26/2010 |
| CONCENTRATION: | 46.87mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GE
Validated NT Sequence
ttattattcg cccggctgat atccgcttgg gtagcgggtc agcccttcct gcatgaccgc ggccaccaac tcgcctccct gggtgaagat cttgccgtgc gtaagtgcgc gacccccgcc tgccgacggc gatgactggt cgtagagcag ccactcgtcg gcccgaaacc cccgcatgaa ccacatcgcg tggtccaggg acgccacctg cagatgctca cgctcggcca ggtgggtgac ctgcgccgaa cccagcagag tcagatcgct catgtaggcc aaggcacaga tgtgcagcac cgggtcatcg ggcagcgggt cgcgatggcg aaaccacacc tgctgctggg atgccttacc cggtagcggc gcgagcaggt cccgcggcac gatgcgcaca tcccactcct cgaactgccg gaatccggcg tcgtcgaaga cccgcaccga cctcaatccg ggtagatcgt cgggcggtgg cgccgccggc atcgcgtcct ggtggctgat gccgttctgc gcggtctgaa acgaagcccc catgctgaag atgacctcgc cgtgctgaac ggcgttgacc cgcctggtca caaacgatcc gccatcgcga gtgcgctcga ccagaaaaac cgtgggctcc tgggcgtccc cggagcgcag aaagtacccg tgcaacgaat ggacctgata gcgcgggtcg accgtgcgca ccgccgacac cagtgactgc ccggccacat ggccgccgaa tgtgcgctgc agaaagcctg attccgggct gaagacacta ccgcgataga tgttgacctc gagttgctcg agatcaagaa tctcctcgat tgccaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCAATC GAGGAGATTC TTGATCTCGA GCAACTCGAG GTCAACATCT ATCGCGGTAG TGTCTTCAGC CCGGAATCAG GCTTTCTGCA GCGCACATTC GGCGGCCATG TGGCCGGGCA GTCACTGGTG TCGGCGGTGC GCACGGTCGA CCCGCGCTAT CAGGTCCATT CGTTGCACGG GTACTTTCTG CGCTCCGGGG ACGCCCAGGA GCCCACGGTT TTTCTGGTCG AGCGCACTCG CGATGGCGGA TCGTTTGTGA CCAGGCGGGT CAACGCCGTT CAGCACGGCG AGGTCATCTT CAGCATGGGG GCTTCGTTTC AGACCGCGCA GAACGGCATC AGCCACCAGG ACGCGATGCC GGCGGCGCCA CCGCCCGACG ATCTACCCGG ATTGAGGTCG GTGCGGGTCT TCGACGACGC CGGATTCCGG CAGTTCGAGG AGTGGGATGT GCGCATCGTG CCGCGGGACC TGCTCGCGCC GCTACCGGGT AAGGCATCCC AGCAGCAGGT GTGGTTTCGC CATCGCGACC CGCTGCCCGA TGACCCGGTG CTGCACATCT GTGCCTTGGC CTACATGAGC GATCTGACTC TGCTGGGTTC GGCGCAGGTC ACCCACCTGG CCGAGCGTGA GCATCTGCAG GTGGCGTCCC TGGACCACGC GATGTGGTTC ATGCGGGGGT TTCGGGCCGA CGAGTGGCTG CTCTACGACC AGTCATCGCC GTCGGCAGGC GGGGGTCGCG CACTTACGCA CGGCAAGATC TTCACCCAGG GAGGCGAGTT GGTGGCCGCG GTCATGCAGG AAGGGCTGAC CCGCTACCCA AGCGGATATC AGCCGGGCGA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA