MysmA.00458.c

Acyl-CoA thioesterase

CENTER ID: MysmA.00458.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00458.c.A1.GE28642 Full length( MysmA.00458.c ) 1 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: YP_886809.1
UniProt: A0QV71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSELPRLLDL DEHDDEDTWT GPASGPDGKR AFGGLRAAQS LAAAARTVGD DRAPTNMHMQ FLRGGDAGEP ARYRVHRVYD GRTASARRVD AHEHGRLLTT ATVSFATPLP GPEHGRRTSV PHDPEALPRT GPPGPAPSLP LDEFDIRIAD DTAGGGFTRR FWWRVTTALP DDALLHTMVA LYITDLYGID PALGVHGLTM RSRSHRSGTT DSSMWFHRRV RADEWNLLEQ TSPAAARGRG IITSSLVQLD GSVVATLVQE GLMAERTTD
NT Sequence
GTGAGCGAAC TGCCACGGCT GCTCGACCTC GACGAGCACG ACGACGAGGA CACCTGGACC GGGCCTGCGA GCGGGCCAGA CGGGAAACGC GCATTCGGTG GGCTGCGCGC GGCGCAGAGC CTGGCCGCCG CGGCTCGCAC CGTGGGCGAT GACCGCGCGC CGACCAACAT GCACATGCAG TTCCTGCGCG GCGGCGACGC CGGCGAGCCC GCCCGCTACC GCGTGCACCG CGTCTACGAC GGACGCACCG CGTCGGCGCG TCGCGTCGAC GCCCACGAGC ACGGCCGGTT GCTCACCACG GCCACGGTGT CGTTCGCGAC GCCGCTGCCC GGGCCGGAGC ACGGCCGCCG CACGAGCGTG CCGCACGATC CCGAGGCGTT GCCGCGCACG GGCCCGCCGG GGCCCGCGCC GTCGCTGCCG CTCGACGAGT TCGACATCCG CATCGCCGAC GACACGGCCG GCGGTGGATT CACCCGGCGG TTCTGGTGGC GGGTCACCAC GGCGCTGCCC GACGATGCGC TGCTGCACAC CATGGTGGCG CTGTACATCA CCGATCTGTA CGGGATCGAT CCCGCGCTCG GGGTGCACGG CCTGACGATG CGTTCACGCA GCCACCGTAG CGGCACGACC GATTCGTCGA TGTGGTTCCA CCGGCGGGTG CGCGCCGACG AGTGGAACCT GCTTGAGCAG ACGTCACCGG CCGCGGCCCG TGGCCGCGGC ATCATCACCT CGAGTCTCGT GCAGCTCGAC GGCAGCGTCG TCGCCACGCT CGTGCAGGAG GGTTTGATGG CCGAGCGGAC GACGGAC
Details for MysmA.00458.c.A1.GE28642
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 31kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MVSELPRLLD LDEHDDEDTW TGPASGPDGK RAFGGLRAAQ SLAAAARTVG DDRAPTNMHM QFLRGGDAGE PARYRVHRVY DGRTASARRV DAHEHGRLLT TATVSFATPL PGPEHGRRTS VPHDPEALPR TGPPGPAPSL PLDEFDIRIA DDTAGGGFTR RFWWRVTTAL PDDALLHTMV ALYITDLYGI DPALGVHGLT MRSRSHRSGX TDSSMWFHRR VRADEWXPA
Validated NT Sequence
annncnannn nnnnggnnac cctggaagct cagacccagg gtcctggttc gatggtgagc gaactgccac ggctgctcga cctcgacgag cacgacgacg aggacacctg gaccgggcct gcgagcgggc cagacgggaa acgcgcattc ggtgggctgc gcgcggcgca gagcctggcc gccgcggctc gcaccgtggg cgatgaccgc gcgccgacca acatgcacat gcagttcctg cgcggcggcg acgccggcga gcccgcccgc taccgcgtgc accgcgtcta cgacggacgc accgcgtcgg cgcgtcgcgt cgacgcccac gagcacggcc ggttgctcac cacggccacg gtgtcgttcg cgacgccgct gcccgggccg gagcacggcc gccgcacgag cgtgccgcac gatcccgagg cgttgccgcg cacgggcccg ccggggcccg cgccgtcgct gccgctcgac gagttcgaca tccgcatcgc cgacgacacg gccggcggtg gattcacccg gcggttctgg tggcgggtca ccacggcgct gcccgacgat gcgctgctgc acaccatggt ggcgctgtac atcaccgatc tgtacgggat cgatcccgcg ctcggggtgc acggnctgac gatgcgttca cgcagccacc gtagcggcnc gaccgattcg tcgatgtggt tccaccggcg ggtgcgcgcc gacgagtggn aacctgcttg agcagacgtc accggccgcg gnccgtggcc gcggnntnat cacctcgagt ctcgtgcagc tcgacggnng cgtcntcncc acgctcgtgc annagggttt natggncnan cgnacnacgg antaataaac nnnnnnaaca agttntgcag nnnnnnttct cgannnncng nntgctnana aanccnaaag nnnntgannt ggctgctnnn accgntganc aatnacnnnc ataaccccnt ggggcaacgg gnntngannn nttttncnnn nnggaggann nnnnancnnn nnnnccnngg anngnnnnnn nnnncnnnnn ngnnnnntcn nnnnngnnnn nagnnnnann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnanngnnnn nnnnnnnnnn tnnntcnnnn nnnnnnnnnn ntnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn annnnnnnnn nncna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSELPRLL DLDEHDDEDT WTGPASGPDG KRAFGGLRAA QSLAAAARTV GDDRAPTNMH MQFLRGGDAG EPARYRVHRV YDGRTASARR VDAHEHGRLL TTATVSFATP LPGPEHGRRT SVPHDPEALP RTGPPGPAPS LPLDEFDIRI ADDTAGGGFT RRFWWRVTTA LPDDALLHTM VALYITDLYG IDPALGVHGL TMRSRSHRSG TTDSSMWFHR RVRADEWNLL EQTSPAAARG RGIITSSLVQ LDGSVVATLV QEGLMAERTT D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgaa ctgccacggc tgctcgacct cgacgagcac gacgacgagg acacctggac cgggcctgcg agcgggccag acgggaaacg cgcattcggt gggctgcgcg cggcgcagag cctggccgcc gcggctcgca ccgtgggcga tgaccgcgcg ccgaccaaca tgcacatgca gttcctgcgc ggcggcgacg ccggcgagcc cgcccgctac cgcgtgcacc gcgtctacga cggacgcacc gcgtcggcgc gtcgcgtcga cgcccacgag cacggccggt tgctcaccac ggccacggtg tcgttcgcga cgccgctgcc cgggccggag cacggccgcc gcacgagcgt gccgcacgat cccgaggcgt tgccgcgcac gggcccgccg gggcccgcgc cgtcgctgcc gctcgacgag ttcgacatcc gcatcgccga cgacacggcc ggcggtggat tcacccggcg gttctggtgg cgggtcacca cggcgctgcc cgacgatgcg ctgctgcaca ccatggtggc gctgtacatc accgatctgt acgggatcga tcccgcgctc ggggtgcacg gcctgacgat gcgttcacgc agccaccgta gcggcacgac cgattcgtcg atgtggttcc accggcgggt gcgcgccgac gagtggaacc tgcttgagca gacgtcaccg gccgcggccc gtggccgcgg catcatcacc tcgagtctcg tgcagctcga cggcagcgtc gtcgccacgc tcgtgcagga gggtttgatg gccgagcgga cgacggacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa