MysmA.00458.b

Palmitoyl-CoA hydrolase

CENTER ID: MysmA.00458.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00458.b.A1.GE28641 Full length( MysmA.00458.b ) 1 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00458.b.A1.PS00561 Full length( MysmA.00458.b ) 1 265

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6046
RefSeq: YP_890427.1
UniProt: A0R5I9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPSWIKGL LAFERDGDVF CIREPEPNTI ERLFGGLIAA QSLGAAGATV DDDKVPQSLH AYFVRGGRYD TDVEFRVTRI RDGRAFATRQ VSASQDGKVI LEMIASFHAP EPGVDWQPRT EPRLEFDAAV PKDPEIDFSD AFEVRTRTSD DSEFAVPPFW IRTREPVEDD PLVRACALTY MSDLGPVPTA LPPTVPLRPD LGFATSLDHS IWFHRPFRPD IWHRYELRAA NHNDSRGLTT GSLIDESGTL IASVCQEALW RLDIS
NT Sequence
GTGAGCGCGC CGAGTTGGAT CAAGGGTCTT CTGGCATTCG AGCGCGACGG TGACGTGTTC TGTATCCGCG AGCCCGAGCC CAACACCATC GAGCGCCTGT TCGGCGGTCT GATCGCCGCG CAGTCGCTGG GCGCCGCGGG TGCCACCGTC GACGACGACA AGGTCCCACA GTCGCTGCAT GCCTACTTCG TCCGCGGCGG CCGGTACGAC ACCGACGTCG AGTTCCGCGT GACGCGCATC CGCGACGGCC GCGCGTTCGC CACGCGCCAG GTCAGTGCCA GCCAGGACGG CAAGGTGATC CTGGAGATGA TCGCGTCGTT CCACGCACCG GAGCCGGGTG TGGACTGGCA GCCCCGGACC GAGCCGCGGC TCGAGTTCGA CGCGGCCGTG CCGAAGGATC CGGAGATCGA CTTCAGCGAC GCGTTCGAGG TTCGCACCCG CACCAGCGAC GATTCTGAGT TCGCGGTGCC GCCGTTCTGG ATCCGCACGC GTGAGCCGGT CGAGGATGAC CCGCTGGTCC GGGCGTGCGC GTTGACCTAC ATGTCCGACC TCGGCCCGGT GCCCACCGCC CTGCCCCCGA CGGTTCCGCT GCGGCCCGAT CTGGGGTTCG CGACGAGCCT CGACCACTCG ATCTGGTTCC ACCGCCCGTT CCGGCCGGAC ATCTGGCACC GCTACGAGTT GCGAGCGGCC AACCACAACG ACTCCCGCGG CCTCACCACG GGCTCGCTGA TCGACGAATC GGGCACGCTG ATCGCCAGTG TGTGCCAGGA GGCGCTGTGG CGCCTTGACA TCTCT
Details for MysmA.00458.b.A1.GE28641
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPSWIK GLLAFERDGD VFCIREPEPN TIERLFGGLI AAQSLGAAGA TVDDDKVPQS LHAYFVRGGR YDTDVEFRVT RIRDGRAFAT RQVSASQDGK VILEMIASFH APEPGVDWQP RTEPRLEFDA AVPKDPEIDF SDAFEVRTRT SDDSEFAVPP FWIRTREPVE DDPLVRACAL TYMSDLGPVP TALPPTVPLR PDLGFATSLD HSIWFHRPFR PDIWHRYELR AAXHNDSRGL TTGSLIDESG TLIASVCQEA LWR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgcgccgag ttggatcaag ggtcttctgg cattcgagcg cgacggtgac gtgttctgta tccgcgagcc cgagcccaac accatcgagc gcctgttcgg cggtctgatc gccgcgcagt cgctgggcgc cgcgggtgcc accgtcgacg acgacaaggt cccacagtcg ctgcatgcct acttcgtccg cggcggccgg tacgacaccg acgtcgagtt ccgcgtgacg cgcatccgcg acggccgcgc gttcgccacg cgccaggtca gtgccagcca ggacggcaag gtgatcctgg agatgatcgc gtcgttccac gcaccggagc cgggtgtgga ctggcagccc cggaccgagc cgcggctcga gttcgacgcg gccgtgccga aggatccgga gatcgacttc agcgacgcgt tcgaggttcg cacccgcacc agcgacgatt ctgagttcgc ggtgccgccg ttctggatcc gcacgcgtga gccggtcgag gatgacccgc tggtccgggc gtgcgcgttg acctacatgt ccgacctcgg cccggtgccc accgccctgc ccccgacggt tccgctgcgg cccgatctgg ggttcgcgac gagcctcgac cactcgatct ggttccaccg cccgttccgg ccggacatct ggcaccgcta cgagttgcga gcggccnacc acaacgactc ccgcggcctc accacgggct cgctgatcga cgaatcgggc acgctgatcg ccagtgtgtg ccaggaggcg ctgtggcgcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPSWIK GLLAFERDGD VFCIREPEPN TIERLFGGLI AAQSLGAAGA TVDDDKVPQS LHAYFVRGGR YDTDVEFRVT RIRDGRAFAT RQVSASQDGK VILEMIASFH APEPGVDWQP RTEPRLEFDA AVPKDPEIDF SDAFEVRTRT SDDSEFAVPP FWIRTREPVE DDPLVRACAL TYMSDLGPVP TALPPTVPLR PDLGFATSLD HSIWFHRPFR PDIWHRYELR AANHNDSRGL TTGSLIDESG TLIASVCQEA LWRLDIS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgcg ccgagttgga tcaagggtct tctggcattc gagcgcgacg gtgacgtgtt ctgtatccgc gagcccgagc ccaacaccat cgagcgcctg ttcggcggtc tgatcgccgc gcagtcgctg ggcgccgcgg gtgccaccgt cgacgacgac aaggtcccac agtcgctgca tgcctacttc gtccgcggcg gccggtacga caccgacgtc gagttccgcg tgacgcgcat ccgcgacggc cgcgcgttcg ccacgcgcca ggtcagtgcc agccaggacg gcaaggtgat cctggagatg atcgcgtcgt tccacgcacc ggagccgggt gtggactggc agccccggac cgagccgcgg ctcgagttcg acgcggccgt gccgaaggat ccggagatcg acttcagcga cgcgttcgag gttcgcaccc gcaccagcga cgattctgag ttcgcggtgc cgccgttctg gatccgcacg cgtgagccgg tcgaggatga cccgctggtc cgggcgtgcg cgttgaccta catgtccgac ctcggcccgg tgcccaccgc cctgcccccg acggttccgc tgcggcccga tctggggttc gcgacgagcc tcgaccactc gatctggttc caccgcccgt tccggccgga catctggcac cgctacgagt tgcgagcggc caaccacaac gactcccgcg gcctcaccac gggctcgctg atcgacgaat cgggcacgct gatcgccagt gtgtgccagg aggcgctgtg gcgccttgac atctctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MysmA.00458.b.A1.PS00561
PURIFICATION DATe: 4/19/2010
CONCENTRATION: 13.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPSWIK GLLAFERDGD VFCIREPEPN TIERLFGGLI AAQSLGAAGA TVDDDKVPQS LHAYFVRGGR YDTDVEFRVT RIRDGRAFAT RQVSASQDGK VILEMIASFH APEPGVDWQP RTEPRLEFDA AVPKDPEIDF SDAFEVRTRT SDDSEFAVPP FWIRTREPVE DDPLVRACAL TYMSDLGPVP TALPPTVPLR PDLGFATSLD HSIWFHRPFR PDIWHRYELR AAXHNDSRGL TTGSLIDESG TLIASVCQEA LWR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgcgccgag ttggatcaag ggtcttctgg cattcgagcg cgacggtgac gtgttctgta tccgcgagcc cgagcccaac accatcgagc gcctgttcgg cggtctgatc gccgcgcagt cgctgggcgc cgcgggtgcc accgtcgacg acgacaaggt cccacagtcg ctgcatgcct acttcgtccg cggcggccgg tacgacaccg acgtcgagtt ccgcgtgacg cgcatccgcg acggccgcgc gttcgccacg cgccaggtca gtgccagcca ggacggcaag gtgatcctgg agatgatcgc gtcgttccac gcaccggagc cgggtgtgga ctggcagccc cggaccgagc cgcggctcga gttcgacgcg gccgtgccga aggatccgga gatcgacttc agcgacgcgt tcgaggttcg cacccgcacc agcgacgatt ctgagttcgc ggtgccgccg ttctggatcc gcacgcgtga gccggtcgag gatgacccgc tggtccgggc gtgcgcgttg acctacatgt ccgacctcgg cccggtgccc accgccctgc ccccgacggt tccgctgcgg cccgatctgg ggttcgcgac gagcctcgac cactcgatct ggttccaccg cccgttccgg ccggacatct ggcaccgcta cgagttgcga gcggccnacc acaacgactc ccgcggcctc accacgggct cgctgatcga cgaatcgggc acgctgatcg ccagtgtgtg ccaggaggcg ctgtggcgcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPSWIK GLLAFERDGD VFCIREPEPN TIERLFGGLI AAQSLGAAGA TVDDDKVPQS LHAYFVRGGR YDTDVEFRVT RIRDGRAFAT RQVSASQDGK VILEMIASFH APEPGVDWQP RTEPRLEFDA AVPKDPEIDF SDAFEVRTRT SDDSEFAVPP FWIRTREPVE DDPLVRACAL TYMSDLGPVP TALPPTVPLR PDLGFATSLD HSIWFHRPFR PDIWHRYELR AANHNDSRGL TTGSLIDESG TLIASVCQEA LWRLDIS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCG CCGAGTTGGA TCAAGGGTCT TCTGGCATTC GAGCGCGACG GTGACGTGTT CTGTATCCGC GAGCCCGAGC CCAACACCAT CGAGCGCCTG TTCGGCGGTC TGATCGCCGC GCAGTCGCTG GGCGCCGCGG GTGCCACCGT CGACGACGAC AAGGTCCCAC AGTCGCTGCA TGCCTACTTC GTCCGCGGCG GCCGGTACGA CACCGACGTC GAGTTCCGCG TGACGCGCAT CCGCGACGGC CGCGCGTTCG CCACGCGCCA GGTCAGTGCC AGCCAGGACG GCAAGGTGAT CCTGGAGATG ATCGCGTCGT TCCACGCACC GGAGCCGGGT GTGGACTGGC AGCCCCGGAC CGAGCCGCGG CTCGAGTTCG ACGCGGCCGT GCCGAAGGAT CCGGAGATCG ACTTCAGCGA CGCGTTCGAG GTTCGCACCC GCACCAGCGA CGATTCTGAG TTCGCGGTGC CGCCGTTCTG GATCCGCACG CGTGAGCCGG TCGAGGATGA CCCGCTGGTC CGGGCGTGCG CGTTGACCTA CATGTCCGAC CTCGGCCCGG TGCCCACCGC CCTGCCCCCG ACGGTTCCGC TGCGGCCCGA TCTGGGGTTC GCGACGAGCC TCGACCACTC GATCTGGTTC CACCGCCCGT TCCGGCCGGA CATCTGGCAC CGCTACGAGT TGCGAGCGGC CAACCACAAC GACTCCCGCG GCCTCACCAC GGGCTCGCTG ATCGACGAAT CGGGCACGCT GATCGCCAGT GTGTGCCAGG AGGCGCTGTG GCGCCTTGAC ATCTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA