MyabA.00458.a

Probable acyl-CoA thioesterase II (TesB2)

CENTER ID: MyabA.00458.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00458.a.A1.GE30306 Full length( MyabA.00458.a ) 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00458.a.A1.PS00883 Full length( MyabA.00458.a ) 1 283
MyabA.00458.a.A1.PS00884 Full length( MyabA.00458.a ) 1 283

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2938
RefSeq: YP_001703624.1
UniProt: B1MCJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADIEEILTL EQLEKDIFRG NVTPSNLRRT FGGQVAGQSL VSAVRTVEPE YLVHSLHGYF LRPGNPNEPT VFLVDRVRDG RSFVTRRVTA IQDGQAIFSM SASFQTLDTG IEHQDLMPPV PDPEDLPDAQ EQDSFNRDLF RQFAEWDIRI VPDELMDRNP RLAAQQRVWF RCRKHLPDDP VLHICALAYM SDLTLLSSSK VPHRDTVLQT ASLDHALWFL RPFRADEWML YDETSPSAGF GRALTQGRIF NRDGTMVAAV VQEGLTRIER NPEQASVARG NMA
NT Sequence
ATGGCCGATA TCGAGGAGAT CCTTACTCTC GAGCAGCTGG AGAAGGACAT CTTCCGGGGC AACGTGACCC CCAGCAATCT TCGTCGCACC TTCGGCGGGC AGGTCGCTGG GCAGTCACTG GTGTCCGCGG TTCGCACCGT GGAACCGGAG TATCTGGTGC ACTCGTTGCA CGGATACTTT CTGCGGCCCG GAAATCCCAA CGAGCCCACC GTGTTTCTCG TCGACCGGGT CCGCGACGGT AGATCGTTCG TAACGCGCCG AGTCACCGCG ATCCAGGACG GGCAGGCCAT CTTCAGCATG TCGGCCTCAT TTCAGACACT GGACACCGGT ATCGAGCATC AGGATCTGAT GCCGCCGGTA CCCGATCCGG AGGATCTGCC CGACGCACAG GAACAGGATT CGTTCAACCG GGATCTTTTC CGGCAGTTCG CCGAGTGGGA TATCCGGATC GTGCCGGACG AGCTCATGGA CAGGAATCCC CGGCTGGCCG CCCAGCAGCG GGTGTGGTTC CGCTGCCGTA AGCACCTGCC CGACGACCCG GTGCTACACA TCTGCGCGCT GGCGTATATG AGCGACCTGA CGTTGCTGAG TTCTTCGAAG GTGCCGCATC GGGATACCGT GCTGCAGACG GCTTCATTGG ACCACGCGCT GTGGTTCCTG CGGCCCTTCC GTGCCGACGA GTGGATGTTG TACGACGAGA CATCCCCGTC GGCAGGGTTC GGGCGCGCGT TGACGCAGGG CCGGATCTTC AACCGGGACG GAACCATGGT GGCTGCGGTG GTGCAAGAGG GGCTTACCCG CATTGAGCGT AACCCCGAGC AGGCGTCGGT GGCCCGAGGG AATATGGCG
Details for MyabA.00458.a.A1.GE30306
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Validated NT Sequence
ttattacgcc atattccctc gggccaccga cgcctgctcg gggttacgct caatgcgggt aagcccctct tgcaccaccg cagccaccat ggttccgtcc cggttgaaga tccggccctg cgtcaacgcg cgcccgaacc ctgccgacgg ggatgtctcg tcgtacaaca tccactcgtc ggcacggaag ggccgcagga accacagcgc gtggtccaat gaagccgtct gcagcacggt atcccgatgc ggcaccttcg aagaactcag caacgtcagg tcgctcatat acgccagcgc gcagatgtgt agcaccgggt cgtcgggcag gtgcttacgg cagcggaacc acacccgctg ctgggcggcc agccggggat tcctgtccat gagctcgtcc ggcacgatcc ggatatccca ctcggcgaac tgccggaaaa gatcccggtt gaacgaatcc tgttcctgtg cgtcgggcag atcctccgga tcgggtaccg gcggcatcag atcctgatgc tcgataccgg tgtccagtgt ctgaaatgag gccgacatgc tgaagatggc ctgcccgtcc tggatcgcgg tgactcggcg cgttacgaac gatctaccgt cgcggacccg gtcgacgaga aacacggtgg gctcgttggg atttccgggc cgcagaaagt atccgtgcaa cgagtgcacc agatactccg gttccacggt gcgaaccgcg gacaccagtg actgcccagc gacctgcccg ccgaaggtgc gacgaagatt gctgggggtc acgttgcccc ggaagatgtc cttctccagc tgctcgagag taaggatctc ctcgatatcg gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatatc gaggagatcc ttactctcga gcagctggag aaggacatct tccggggcaa cgtgaccccc agcaatcttc gtcgcacctt cggcgggcag gtcgctgggc agtcactggt gtccgcggtt cgcaccgtgg aaccggagta tctggtgcac tcgttgcacg gatactttct gcggcccgga aatcccaacg agcccaccgt gtttctcgtc gaccgggtcc gcgacggtag atcgttcgta acgcgccgag tcaccgcgat ccaggacggg caggccatct tcagcatgtc ggcctcattt cagacactgg acaccggtat cgagcatcag gatctgatgc cgccggtacc cgatccggag gatctgcccg acgcacagga acaggattcg ttcaaccggg atcttttccg gcagttcgcc gagtgggata tccggatcgt gccggacgag ctcatggaca ggaatccccg gctggccgcc cagcagcggg tgtggttccg ctgccgtaag cacctgcccg acgacccggt gctacacatc tgcgcgctgg cgtatatgag cgacctgacg ttgctgagtt cttcgaaggt gccgcatcgg gataccgtgc tgcagacggc ttcattggac cacgcgctgt ggttcctgcg gcccttccgt gccgacgagt ggatgttgta cgacgagaca tccccgtcgg cagggttcgg gcgcgcgttg acgcagggcc ggatcttcaa ccgggacgga accatggtgg ctgcggtggt gcaagagggg cttacccgca ttgagcgtaa ccccgagcag gcgtcggtgg cccgagggaa tatggcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyabA.00458.a.A1.PS00883
PURIFICATION DATe: 11/29/2010
CONCENTRATION: 53.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Validated NT Sequence
ttattacgcc atattccctc gggccaccga cgcctgctcg gggttacgct caatgcgggt aagcccctct tgcaccaccg cagccaccat ggttccgtcc cggttgaaga tccggccctg cgtcaacgcg cgcccgaacc ctgccgacgg ggatgtctcg tcgtacaaca tccactcgtc ggcacggaag ggccgcagga accacagcgc gtggtccaat gaagccgtct gcagcacggt atcccgatgc ggcaccttcg aagaactcag caacgtcagg tcgctcatat acgccagcgc gcagatgtgt agcaccgggt cgtcgggcag gtgcttacgg cagcggaacc acacccgctg ctgggcggcc agccggggat tcctgtccat gagctcgtcc ggcacgatcc ggatatccca ctcggcgaac tgccggaaaa gatcccggtt gaacgaatcc tgttcctgtg cgtcgggcag atcctccgga tcgggtaccg gcggcatcag atcctgatgc tcgataccgg tgtccagtgt ctgaaatgag gccgacatgc tgaagatggc ctgcccgtcc tggatcgcgg tgactcggcg cgttacgaac gatctaccgt cgcggacccg gtcgacgaga aacacggtgg gctcgttggg atttccgggc cgcagaaagt atccgtgcaa cgagtgcacc agatactccg gttccacggt gcgaaccgcg gacaccagtg actgcccagc gacctgcccg ccgaaggtgc gacgaagatt gctgggggtc acgttgcccc ggaagatgtc cttctccagc tgctcgagag taaggatctc ctcgatatcg gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATATC GAGGAGATCC TTACTCTCGA GCAGCTGGAG AAGGACATCT TCCGGGGCAA CGTGACCCCC AGCAATCTTC GTCGCACCTT CGGCGGGCAG GTCGCTGGGC AGTCACTGGT GTCCGCGGTT CGCACCGTGG AACCGGAGTA TCTGGTGCAC TCGTTGCACG GATACTTTCT GCGGCCCGGA AATCCCAACG AGCCCACCGT GTTTCTCGTC GACCGGGTCC GCGACGGTAG ATCGTTCGTA ACGCGCCGAG TCACCGCGAT CCAGGACGGG CAGGCCATCT TCAGCATGTC GGCCTCATTT CAGACACTGG ACACCGGTAT CGAGCATCAG GATCTGATGC CGCCGGTACC CGATCCGGAG GATCTGCCCG ACGCACAGGA ACAGGATTCG TTCAACCGGG ATCTTTTCCG GCAGTTCGCC GAGTGGGATA TCCGGATCGT GCCGGACGAG CTCATGGACA GGAATCCCCG GCTGGCCGCC CAGCAGCGGG TGTGGTTCCG CTGCCGTAAG CACCTGCCCG ACGACCCGGT GCTACACATC TGCGCGCTGG CGTATATGAG CGACCTGACG TTGCTGAGTT CTTCGAAGGT GCCGCATCGG GATACCGTGC TGCAGACGGC TTCATTGGAC CACGCGCTGT GGTTCCTGCG GCCCTTCCGT GCCGACGAGT GGATGTTGTA CGACGAGACA TCCCCGTCGG CAGGGTTCGG GCGCGCGTTG ACGCAGGGCC GGATCTTCAA CCGGGACGGA ACCATGGTGG CTGCGGTGGT GCAAGAGGGG CTTACCCGCA TTGAGCGTAA CCCCGAGCAG GCGTCGGTGG CCCGAGGGAA TATGGCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for MyabA.00458.a.A1.PS00884
PURIFICATION DATe: 11/29/2010
CONCENTRATION: 46.48mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Validated NT Sequence
ttattacgcc atattccctc gggccaccga cgcctgctcg gggttacgct caatgcgggt aagcccctct tgcaccaccg cagccaccat ggttccgtcc cggttgaaga tccggccctg cgtcaacgcg cgcccgaacc ctgccgacgg ggatgtctcg tcgtacaaca tccactcgtc ggcacggaag ggccgcagga accacagcgc gtggtccaat gaagccgtct gcagcacggt atcccgatgc ggcaccttcg aagaactcag caacgtcagg tcgctcatat acgccagcgc gcagatgtgt agcaccgggt cgtcgggcag gtgcttacgg cagcggaacc acacccgctg ctgggcggcc agccggggat tcctgtccat gagctcgtcc ggcacgatcc ggatatccca ctcggcgaac tgccggaaaa gatcccggtt gaacgaatcc tgttcctgtg cgtcgggcag atcctccgga tcgggtaccg gcggcatcag atcctgatgc tcgataccgg tgtccagtgt ctgaaatgag gccgacatgc tgaagatggc ctgcccgtcc tggatcgcgg tgactcggcg cgttacgaac gatctaccgt cgcggacccg gtcgacgaga aacacggtgg gctcgttggg atttccgggc cgcagaaagt atccgtgcaa cgagtgcacc agatactccg gttccacggt gcgaaccgcg gacaccagtg actgcccagc gacctgcccg ccgaaggtgc gacgaagatt gctgggggtc acgttgcccc ggaagatgtc cttctccagc tgctcgagag taaggatctc ctcgatatcg gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADIEEILT LEQLEKDIFR GNVTPSNLRR TFGGQVAGQS LVSAVRTVEP EYLVHSLHGY FLRPGNPNEP TVFLVDRVRD GRSFVTRRVT AIQDGQAIFS MSASFQTLDT GIEHQDLMPP VPDPEDLPDA QEQDSFNRDL FRQFAEWDIR IVPDELMDRN PRLAAQQRVW FRCRKHLPDD PVLHICALAY MSDLTLLSSS KVPHRDTVLQ TASLDHALWF LRPFRADEWM LYDETSPSAG FGRALTQGRI FNRDGTMVAA VVQEGLTRIE RNPEQASVAR GNMA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATATC GAGGAGATCC TTACTCTCGA GCAGCTGGAG AAGGACATCT TCCGGGGCAA CGTGACCCCC AGCAATCTTC GTCGCACCTT CGGCGGGCAG GTCGCTGGGC AGTCACTGGT GTCCGCGGTT CGCACCGTGG AACCGGAGTA TCTGGTGCAC TCGTTGCACG GATACTTTCT GCGGCCCGGA AATCCCAACG AGCCCACCGT GTTTCTCGTC GACCGGGTCC GCGACGGTAG ATCGTTCGTA ACGCGCCGAG TCACCGCGAT CCAGGACGGG CAGGCCATCT TCAGCATGTC GGCCTCATTT CAGACACTGG ACACCGGTAT CGAGCATCAG GATCTGATGC CGCCGGTACC CGATCCGGAG GATCTGCCCG ACGCACAGGA ACAGGATTCG TTCAACCGGG ATCTTTTCCG GCAGTTCGCC GAGTGGGATA TCCGGATCGT GCCGGACGAG CTCATGGACA GGAATCCCCG GCTGGCCGCC CAGCAGCGGG TGTGGTTCCG CTGCCGTAAG CACCTGCCCG ACGACCCGGT GCTACACATC TGCGCGCTGG CGTATATGAG CGACCTGACG TTGCTGAGTT CTTCGAAGGT GCCGCATCGG GATACCGTGC TGCAGACGGC TTCATTGGAC CACGCGCTGT GGTTCCTGCG GCCCTTCCGT GCCGACGAGT GGATGTTGTA CGACGAGACA TCCCCGTCGG CAGGGTTCGG GCGCGCGTTG ACGCAGGGCC GGATCTTCAA CCGGGACGGA ACCATGGTGG CTGCGGTGGT GCAAGAGGGG CTTACCCGCA TTGAGCGTAA CCCCGAGCAG GCGTCGGTGG CCCGAGGGAA TATGGCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA