SSGCID: Target Page

MyavA.00458.b

Acyl-CoA thioesterase II

CENTER ID:	MyavA.00458.b
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00458.b.A1.GE28546	Full length( MyavA.00458.b )		1	278

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3409
RefSeq:	YP_882665.1
UniProt:	A0A0H2ZZS6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIEQILDLE QLEVNIYRGG VFSPDSGYFQ RTFGGHVAGQ SLVSAVRTVD PRYQVHSLHG YFLRPGDAKE RTVFIVERTR DGGSFATRRV NAIQHGEIIF SMGASFQTDQ EGIHHQDAMP AAPPPDGLPG LDSIKVFDDA GFKQFEEWDV CIVPREKLQL TPGKASQQQV WFRHRDPLPD DPVLHICALA YMSDLTLLGS AQVTHLDVRE HLQVASLDHA MWFMRAFRAD EWLLYDQSSP SANGGRSLCQ GKIFTQSGEM VAAVMQEGLT RFQRGYRQ

NT Sequence

GTGTCGATCG AGCAGATCCT TGATCTCGAG CAACTCGAGG TCAACATCTA CCGCGGGGGC GTCTTCAGCC CGGATTCCGG ATATTTCCAG CGCACCTTCG GCGGCCATGT GGCCGGCCAG TCGCTGGTGT CGGCGGTGCG CACGGTCGAC CCGCGCTACC AGGTGCACTC GCTGCACGGA TACTTCCTGC GGCCCGGGGA CGCCAAGGAG CGCACGGTGT TCATCGTGGA GCGCACCCGC GACGGCGGCT CGTTTGCCAC CCGGCGGGTC AACGCCATTC AGCACGGCGA GATCATCTTC TCCATGGGCG CGTCGTTCCA GACCGACCAG GAGGGCATCC ACCACCAGGA CGCCATGCCG GCCGCGCCGC CCCCGGACGG CCTGCCCGGG CTGGACTCGA TCAAGGTGTT CGACGACGCC GGGTTCAAGC AGTTCGAGGA GTGGGACGTC TGCATCGTGC CGCGGGAGAA GCTGCAGCTG ACCCCGGGCA AGGCGTCCCA GCAGCAGGTG TGGTTCCGCC ACCGCGACCC GTTGCCCGAC GACCCGGTGC TGCACATCTG CGCGCTGGCC TACATGAGCG ACCTGACGCT GCTGGGCTCG GCGCAGGTCA CCCACCTCGA CGTGCGCGAG CACCTGCAGG TGGCGTCGCT GGACCACGCC ATGTGGTTCA TGCGGGCGTT CCGCGCCGAC GAGTGGCTGC TCTACGACCA GTCCTCGCCG TCGGCCAACG GCGGCCGCTC GCTGTGCCAG GGCAAGATCT TCACCCAGTC CGGCGAGATG GTGGCCGCGG TCATGCAGGA GGGGCTGACC CGCTTCCAGC GGGGATATCG GCAG

Details for MyavA.00458.b.A1.GE28546

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSIEQILD LEQLEVNIYR GGVFSPDSGY FQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRPGDA KERTVFIVER TRDGGSFATR RVNAIQHGEI IFSMGASFQT DQEGIHHQDA MPAAPPPDGL PGLDSIKVFD DAGFKQFEEW DVCIVPREKL QLTPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLDV REHLQVASLD HAMWFMRAFR ADEWLLYDQS SPSANGGRSL CQGKIFTQSG EMVAAVMQEG LTRFQRGYRQ

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt cgatcgagca gatccttgat ctcgagcaac tcgaggtcaa catctaccgc gggggcgtct tcagcccgga ttccggatat ttccagcgca ccttcggcgg ccatgtggcc ggccagtcgc tggtgtcggc ggtgcgcacg gtcgacccgc gctaccaggt gcactcgctg cacggatact tcctgcggcc cggggacgcc aaggagcgca cggtgttcat cgtggagcgc acccgcgacg gcggctcgtt tgccacccgg cgggtcaacg ccattcagca cggcgagatc atcttctcca tgggcgcgtc gttccagacc gaccaggagg gcatccacca ccaggacgcc atgccggccg cgccgccccc ggacggcctg cccgggctgg actcgatcaa ggtgttcgac gacgccgggt tcaagcagtt cgaggagtgg gacgtctgca tcgtgccgcg ggagaagctg cagctgaccc cgggcaaggc gtcccagcag caggtgtggt tccgccaccg cgacccgttg cccgacgacc cggtgctgca catctgcgcg ctggcctaca tgagcgacct gacgctgctg ggctcggcgc aggtcaccca cctcgacgtg cgcgagcacc tgcaggtggc gtcgctggac cacgccatgt ggttcatgcg ggcgttccgc gccgacgagt ggctgctcta cgaccagtcc tcgccgtcgg ccaacggcgg ccgctcgctg tgccagggca agatcttcac ccagtccggc gagatggtgg ccgcggtcat gcaggagggg ctgacccgct tccagcgggg atatcggcag taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcgatc gagcagatcc ttgatctcga gcaactcgag gtcaacatct accgcggggg cgtcttcagc ccggattccg gatatttcca gcgcaccttc ggcggccatg tggccggcca gtcgctggtg tcggcggtgc gcacggtcga cccgcgctac caggtgcact cgctgcacgg atacttcctg cggcccgggg acgccaagga gcgcacggtg ttcatcgtgg agcgcacccg cgacggcggc tcgtttgcca cccggcgggt caacgccatt cagcacggcg agatcatctt ctccatgggc gcgtcgttcc agaccgacca ggagggcatc caccaccagg acgccatgcc ggccgcgccg cccccggacg gcctgcccgg gctggactcg atcaaggtgt tcgacgacgc cgggttcaag cagttcgagg agtgggacgt ctgcatcgtg ccgcgggaga agctgcagct gaccccgggc aaggcgtccc agcagcaggt gtggttccgc caccgcgacc cgttgcccga cgacccggtg ctgcacatct gcgcgctggc ctacatgagc gacctgacgc tgctgggctc ggcgcaggtc acccacctcg acgtgcgcga gcacctgcag gtggcgtcgc tggaccacgc catgtggttc atgcgggcgt tccgcgccga cgagtggctg ctctacgacc agtcctcgcc gtcggccaac ggcggccgct cgctgtgcca gggcaagatc ttcacccagt ccggcgagat ggtggccgcg gtcatgcagg aggggctgac ccgcttccag cggggatatc ggcagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa