SSGCID: Target Page

MythA.00458.b

Acyl-CoA thioesterase II (A0QWG9 homolog)

CENTER ID:	MythA.00458.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00458.b.A1.GE28737	Full length( MythA.00458.b )		1	293

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00458.b.A1.PS00673	Full length( MythA.00458.b )		1	293

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.838
RefSeq:	WP_003924315.1
UniProt:	G7CCZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIEEILDLE QLEVNIYRGK VFSPESGFLQ RTXXXXXXXX XXXLVSAVRT VDPKFQVHSL HGYFLLPGDA RAPTVYFVER LRDGGSFCTR RVSAIQHGEV IFSMSASFQT DQSGIEHQDA MPEAPPPDDL PGFRSARAFD DAGFAQFDEW DVRVVPRDKL KRVAGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVNHVAE RPHLQVASLD HAMWFMRPFR ADEWLLYDQS SPSACGGRAL TQGQIFNQYG EMVAAVMQEG LTRYPRNFTR TQQIQTEQTQ TRP

NT Sequence

ATGGCGATCG AGGAGATCCT CGATCTCGAG CAGCTCGAGG TCAACATCTA CCGCGGGAAG GTGTTCAGCC CGGAGTCCGG CTTCCTGCAA CGCACGTNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TGGTGTCGGC GGTGCGCACC GTCGACCCGA AGTTCCAGGT GCACTCGCTG CACGGCTATT TCCTGCTCCC CGGGGACGCC CGGGCGCCGA CGGTGTACTT CGTGGAGCGG CTGCGGGACG GGGGATCGTT CTGCACCCGC CGGGTCAGCG CGATCCAGCA CGGCGAGGTG ATCTTCTCGA TGTCGGCGTC GTTCCAGACC GACCAGAGCG GGATCGAGCA CCAGGACGCG ATGCCCGAGG CGCCGCCCCC GGACGATCTG CCCGGTTTCC GGTCCGCCCG GGCGTTCGAC GACGCCGGCT TCGCCCAGTT CGACGAATGG GATGTGCGGG TGGTGCCGCG CGACAAGTTG AAGCGCGTCG CCGGCAAGGC GTCCCAACAG CAGGTGTGGT TCCGGCACCG GGACCCGCTG CCGGACGACC CGGTGCTGCA CATCTGCGCG CTGGCCTACA TGAGCGACCT GACCCTGCTG GGGTCGGCAC AGGTCAACCA CGTCGCGGAG CGGCCCCACC TGCAGGTGGC GTCGCTGGAT CACGCGATGT GGTTCATGCG GCCGTTCCGC GCCGACGAGT GGCTGCTCTA CGACCAGTCC TCGCCGTCGG CCTGCGGCGG CCGGGCGCTG ACCCAGGGCC AGATCTTCAA CCAGTACGGG GAGATGGTGG CCGCGGTGAT GCAGGAGGGG CTCACCCGCT ACCCGCGCAA CTTCACCAGG ACCCAGCAGA TCCAGACCGA GCAGACGCAG ACCCGGCCG

Details for MythA.00458.b.A1.GE28737

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAIEEILDL EQLEVNIYRG KVFSPESGFL QRTFGGHVAG QSLVSAVRTV DPKFQVHSLH GYFLLPGDAR APTVYFVERL RDGGSFCTRR VSAIQHGEVI FSMSASFQTD QSGIEHQDAM PEAPPPDDLP GFRSARAFDD AGFAQFDEWD VRVVPRDKLK RVAGKASQQQ VWFRHRDPLP DDPVLHICAL AYMSDLTLLG SAQVNHVAER PHLQVASLDH AMWFMRPFRA DEWLLYDQSS PSACGGRALT QGQIFNQYGE MVAAVMQEGL TRYPRNFTRT QQIQTEQTQT RP

Validated NT Sequence

tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgat cgaggagatc ctcgatctcg agcagctcga ggtcaacatc taccgcggga aggtgttcag cccggagtcc ggcttcctgc aacgcacgtt cggcggtcac gtcgccggcc agtcgctggt gtcggcggtg cgcaccgtcg acccgaagtt ccaggtgcac tcgctgcacg gctatttcct gctccccggg gacgcccggg cgccgacggt gtacttcgtg gagcggctgc gggacggggg atcgttctgc acccgccggg tcagcgcgat ccagcacggc gaggtgatct tctcgatgtc ggcgtcgttc cagaccgacc agagcgggat cgagcaccag gacgcgatgc ccgaggcgcc gcccccggac gatctgcccg gtttccggtc cgcccgggcg ttcgacgacg ccggcttcgc ccagttcgac gaatgggatg tgcgggtggt gccgcgcgac aagttgaagc gcgtcgccgg caaggcgtcc caacagcagg tgtggttccg gcaccgggac ccgctgccgg acgacccggt gctgcacatc tgcgcgctgg cctacatgag cgacctgacc ctgctggggt cggcacaggt caaccacgtc gcggagcggc cccacctgca ggtggcgtcg ctggatcacg cgatgtggtt catgcggccg ttccgcgccg acgagtggct gctctacgac cagtcctcgc cgtcggcctg cggcggccgg gcgctgaccc agggccagat cttcaaccag tacggggaga tggtggccgc ggtgatgcag gaggggctca cccgctaccc gcgcaacttc accaggaccc agcagatcca gaccgagcag acgcagaccc ggccgtaata a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAIEEILDL EQLEVNIYRG KVFSPESGFL QRTXXXXXXX XXXXLVSAVR TVDPKFQVHS LHGYFLLPGD ARAPTVYFVE RLRDGGSFCT RRVSAIQHGE VIFSMSASFQ TDQSGIEHQD AMPEAPPPDD LPGFRSARAF DDAGFAQFDE WDVRVVPRDK LKRVAGKASQ QQVWFRHRDP LPDDPVLHIC ALAYMSDLTL LGSAQVNHVA ERPHLQVASL DHAMWFMRPF RADEWLLYDQ SSPSACGGRA LTQGQIFNQY GEMVAAVMQE GLTRYPRNFT RTQQIQTEQT QTRP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgatcgag gagatcctcg atctcgagca gctcgaggtc aacatctacc gcgggaaggt gttcagcccg gagtccggct tcctgcaacg cacgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg gtgtcggcgg tgcgcaccgt cgacccgaag ttccaggtgc actcgctgca cggctatttc ctgctccccg gggacgcccg ggcgccgacg gtgtacttcg tggagcggct gcgggacggg ggatcgttct gcacccgccg ggtcagcgcg atccagcacg gcgaggtgat cttctcgatg tcggcgtcgt tccagaccga ccagagcggg atcgagcacc aggacgcgat gcccgaggcg ccgcccccgg acgatctgcc cggtttccgg tccgcccggg cgttcgacga cgccggcttc gcccagttcg acgaatggga tgtgcgggtg gtgccgcgcg acaagttgaa gcgcgtcgcc ggcaaggcgt cccaacagca ggtgtggttc cggcaccggg acccgctgcc ggacgacccg gtgctgcaca tctgcgcgct ggcctacatg agcgacctga ccctgctggg gtcggcacag gtcaaccacg tcgcggagcg gccccacctg caggtggcgt cgctggatca cgcgatgtgg ttcatgcggc cgttccgcgc cgacgagtgg ctgctctacg accagtcctc gccgtcggcc tgcggcggcc gggcgctgac ccagggccag atcttcaacc agtacgggga gatggtggcc gcggtgatgc aggaggggct cacccgctac ccgcgcaact tcaccaggac ccagcagatc cagaccgagc agacgcagac ccggccgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MythA.00458.b.A1.PS00673

PURIFICATION DATe:	7/14/2010
CONCENTRATION:	47.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGATCGAG GAGATCCTCG ATCTCGAGCA GCTCGAGGTC AACATCTACC GCGGGAAGGT GTTCAGCCCG GAGTCCGGCT TCCTGCAACG CACGTNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCTG GTGTCGGCGG TGCGCACCGT CGACCCGAAG TTCCAGGTGC ACTCGCTGCA CGGCTATTTC CTGCTCCCCG GGGACGCCCG GGCGCCGACG GTGTACTTCG TGGAGCGGCT GCGGGACGGG GGATCGTTCT GCACCCGCCG GGTCAGCGCG ATCCAGCACG GCGAGGTGAT CTTCTCGATG TCGGCGTCGT TCCAGACCGA CCAGAGCGGG ATCGAGCACC AGGACGCGAT GCCCGAGGCG CCGCCCCCGG ACGATCTGCC CGGTTTCCGG TCCGCCCGGG CGTTCGACGA CGCCGGCTTC GCCCAGTTCG ACGAATGGGA TGTGCGGGTG GTGCCGCGCG ACAAGTTGAA GCGCGTCGCC GGCAAGGCGT CCCAACAGCA GGTGTGGTTC CGGCACCGGG ACCCGCTGCC GGACGACCCG GTGCTGCACA TCTGCGCGCT GGCCTACATG AGCGACCTGA CCCTGCTGGG GTCGGCACAG GTCAACCACG TCGCGGAGCG GCCCCACCTG CAGGTGGCGT CGCTGGATCA CGCGATGTGG TTCATGCGGC CGTTCCGCGC CGACGAGTGG CTGCTCTACG ACCAGTCCTC GCCGTCGGCC TGCGGCGGCC GGGCGCTGAC CCAGGGCCAG ATCTTCAACC AGTACGGGGA GATGGTGGCC GCGGTGATGC AGGAGGGGCT CACCCGCTAC CCGCGCAACT TCACCAGGAC CCAGCAGATC CAGACCGAGC AGACGCAGAC CCGGCCGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA