MyulA.00458.b

Acyl-CoA thioesterase II TesB2

CENTER ID: MyulA.00458.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00458.b.A1.GE29697 Full length( MyulA.00458.b ) 1 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00458.b.A1.PS00767 Full length( MyulA.00458.b ) 1 281
MyulA.00458.b.A1.PS00797 Full length( MyulA.00458.b ) 1 281

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3787
RefSeq: YP_906927.1
UniProt: A0PSY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIEEILDLE QLEVNIYRGS VFSPESGFLQ RTFGGHVAGQ SLVSAVRTVD PRYQVHSLHG YFLRSGDAQE PTVFLVERTR DGGSFVTRRV NAVQHGEVIF SMGASFQTAQ NGISHQDAMP AAPPPDDLPG LRSVRVFDDA GFRQFEEWDV RIVPRDLLAP LPGKASQQQV WFRHRDPLPD DPVLHICALA YMSDLTLLGS AQVTHLAERE HLQVASLDHA MWFMRGFRAD EWLLYDQSSP SAGGGRALTH GKIFTQGGEL VAAVMQEGLT RYPSGYQPGE N
NT Sequence
GTGGCAATCG AGGAGATTCT TGATCTCGAG CAACTCGAGG TCAACATCTA TCGCGGTAGT GTCTTCAGCC CGGAATCAGG CTTTCTGCAG CGCACATTCG GCGGCCATGT GGCCGGGCAG TCACTGGTGT CGGCGGTGCG CACGGTCGAC CCGCGCTATC AGGTCCACTC GTTGCACGGG TACTTTCTGC GCTCCGGGGA CGCCCAGGAG CCCACGGTTT TTCTGGTCGA GCGCACTCGC GATGGCGGAT CGTTTGTGAC CAGGCGGGTC AACGCCGTTC AGCACGGCGA GGTCATCTTC AGCATGGGGG CTTCGTTTCA GACCGCGCAG AACGGCATCA GCCACCAGGA CGCGATGCCG GCGGCGCCAC CACCCGACGA TCTACCCGGA TTGAGGTCGG TGCGGGTCTT CGACGACGCC GGGTTCCGGC AGTTCGAGGA GTGGGATGTG CGCATCGTGC CGCGGGACCT GCTCGCGCCG CTACCGGGTA AGGCATCCCA GCAGCAGGTG TGGTTTCGCC ATCGCGACCC GCTGCCCGAT GACCCGGTGC TGCACATCTG TGCCTTGGCC TACATGAGCG ATCTGACCCT GCTGGGTTCG GCGCAGGTCA CCCACCTGGC GGAGCGTGAG CATCTGCAGG TGGCGTCCCT GGACCACGCG ATGTGGTTCA TGCGGGGGTT TCGGGCCGAC GAGTGGCTGC TCTACGACCA GTCATCGCCG TCGGCAGGCG GGGGTCGCGC ACTTACGCAC GGCAAGATCT TCACCCAGGG AGGCGAGTTG GTGGCCGCGG TCATGCAGGA AGGGCTGACC CGCTACCCAA GCGGATATCA GCCGGGCGAA AAT
Details for MyulA.00458.b.A1.GE29697
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP G
Validated NT Sequence
cgcccggctg atatccgctt gggtagcggg tcagcccttc ctgcatgacc gcggccacca actcgcctcc ctgggtgaag atcttgccgt gcgtaagtgc gcgacccccg cctgccgacg gcgatgactg gtcgtagagc agccactcgt cggcccgaaa cccccgcatg aaccacatcg cgtggtccag ggacgccacc tgcagatgct cacgctccgc caggtgggtg acctgcgccg aacccagcag ggtcagatcg ctcatgtagg ccaaggcaca gatgtgcagc accgggtcat cgggcagcgg gtcgcgatgg cgaaaccaca cctgctgctg ggatgcctta cccggtagcg gcgcgagcag gtcccgcggc acgatgcgca catcccactc ctcgaactgc cggaacccgg cgtcgtcgaa gacccgcacc gacctcaatc cgggtagatc gtcgggtggt ggcgccgccg gcatcgcgtc ctggtggctg atgccgttct gcgcggtctg aaacgaagcc cccatgctga agatgacctc gccgtgctga acggcgttga cccgcctggt cacaaacgat ccgccatcgc gagtgcgctc gaccagaaaa accgtgggct cctgggcgtc cccggagcgc agaaagtacc cgtgcaacga gtggacctga tagcgcgggt cgaccgtgcg caccgccgac accagtgact gcccggccac atggccgccg aatgtgcgct gcagaaagcc tgattccggg ctgaagacac taccgcgata gatgttgacc tcgagttgct cgagatcaag aatctcctcg attgccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GEN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcaatc gaggagattc ttgatctcga gcaactcgag gtcaacatct atcgcggtag tgtcttcagc ccggaatcag gctttctgca gcgcacattc ggcggccatg tggccgggca gtcactggtg tcggcggtgc gcacggtcga cccgcgctat caggtccact cgttgcacgg gtactttctg cgctccgggg acgcccagga gcccacggtt tttctggtcg agcgcactcg cgatggcgga tcgtttgtga ccaggcgggt caacgccgtt cagcacggcg aggtcatctt cagcatgggg gcttcgtttc agaccgcgca gaacggcatc agccaccagg acgcgatgcc ggcggcgcca ccacccgacg atctacccgg attgaggtcg gtgcgggtct tcgacgacgc cgggttccgg cagttcgagg agtgggatgt gcgcatcgtg ccgcgggacc tgctcgcgcc gctaccgggt aaggcatccc agcagcaggt gtggtttcgc catcgcgacc cgctgcccga tgacccggtg ctgcacatct gtgccttggc ctacatgagc gatctgaccc tgctgggttc ggcgcaggtc acccacctgg cggagcgtga gcatctgcag gtggcgtccc tggaccacgc gatgtggttc atgcgggggt ttcgggccga cgagtggctg ctctacgacc agtcatcgcc gtcggcaggc gggggtcgcg cacttacgca cggcaagatc ttcacccagg gaggcgagtt ggtggccgcg gtcatgcagg aagggctgac ccgctaccca agcggatatc agccgggcga aaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyulA.00458.b.A1.PS00767
PURIFICATION DATe: 9/13/2010
CONCENTRATION: 30.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP G
Validated NT Sequence
cgcccggctg atatccgctt gggtagcggg tcagcccttc ctgcatgacc gcggccacca actcgcctcc ctgggtgaag atcttgccgt gcgtaagtgc gcgacccccg cctgccgacg gcgatgactg gtcgtagagc agccactcgt cggcccgaaa cccccgcatg aaccacatcg cgtggtccag ggacgccacc tgcagatgct cacgctccgc caggtgggtg acctgcgccg aacccagcag ggtcagatcg ctcatgtagg ccaaggcaca gatgtgcagc accgggtcat cgggcagcgg gtcgcgatgg cgaaaccaca cctgctgctg ggatgcctta cccggtagcg gcgcgagcag gtcccgcggc acgatgcgca catcccactc ctcgaactgc cggaacccgg cgtcgtcgaa gacccgcacc gacctcaatc cgggtagatc gtcgggtggt ggcgccgccg gcatcgcgtc ctggtggctg atgccgttct gcgcggtctg aaacgaagcc cccatgctga agatgacctc gccgtgctga acggcgttga cccgcctggt cacaaacgat ccgccatcgc gagtgcgctc gaccagaaaa accgtgggct cctgggcgtc cccggagcgc agaaagtacc cgtgcaacga gtggacctga tagcgcgggt cgaccgtgcg caccgccgac accagtgact gcccggccac atggccgccg aatgtgcgct gcagaaagcc tgattccggg ctgaagacac taccgcgata gatgttgacc tcgagttgct cgagatcaag aatctcctcg attgccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GEN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCAATC GAGGAGATTC TTGATCTCGA GCAACTCGAG GTCAACATCT ATCGCGGTAG TGTCTTCAGC CCGGAATCAG GCTTTCTGCA GCGCACATTC GGCGGCCATG TGGCCGGGCA GTCACTGGTG TCGGCGGTGC GCACGGTCGA CCCGCGCTAT CAGGTCCACT CGTTGCACGG GTACTTTCTG CGCTCCGGGG ACGCCCAGGA GCCCACGGTT TTTCTGGTCG AGCGCACTCG CGATGGCGGA TCGTTTGTGA CCAGGCGGGT CAACGCCGTT CAGCACGGCG AGGTCATCTT CAGCATGGGG GCTTCGTTTC AGACCGCGCA GAACGGCATC AGCCACCAGG ACGCGATGCC GGCGGCGCCA CCACCCGACG ATCTACCCGG ATTGAGGTCG GTGCGGGTCT TCGACGACGC CGGGTTCCGG CAGTTCGAGG AGTGGGATGT GCGCATCGTG CCGCGGGACC TGCTCGCGCC GCTACCGGGT AAGGCATCCC AGCAGCAGGT GTGGTTTCGC CATCGCGACC CGCTGCCCGA TGACCCGGTG CTGCACATCT GTGCCTTGGC CTACATGAGC GATCTGACCC TGCTGGGTTC GGCGCAGGTC ACCCACCTGG CGGAGCGTGA GCATCTGCAG GTGGCGTCCC TGGACCACGC GATGTGGTTC ATGCGGGGGT TTCGGGCCGA CGAGTGGCTG CTCTACGACC AGTCATCGCC GTCGGCAGGC GGGGGTCGCG CACTTACGCA CGGCAAGATC TTCACCCAGG GAGGCGAGTT GGTGGCCGCG GTCATGCAGG AAGGGCTGAC CCGCTACCCA AGCGGATATC AGCCGGGCGA AAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for MyulA.00458.b.A1.PS00797
PURIFICATION DATe: 10/8/2010
CONCENTRATION: 9.01mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP G
Validated NT Sequence
cgcccggctg atatccgctt gggtagcggg tcagcccttc ctgcatgacc gcggccacca actcgcctcc ctgggtgaag atcttgccgt gcgtaagtgc gcgacccccg cctgccgacg gcgatgactg gtcgtagagc agccactcgt cggcccgaaa cccccgcatg aaccacatcg cgtggtccag ggacgccacc tgcagatgct cacgctccgc caggtgggtg acctgcgccg aacccagcag ggtcagatcg ctcatgtagg ccaaggcaca gatgtgcagc accgggtcat cgggcagcgg gtcgcgatgg cgaaaccaca cctgctgctg ggatgcctta cccggtagcg gcgcgagcag gtcccgcggc acgatgcgca catcccactc ctcgaactgc cggaacccgg cgtcgtcgaa gacccgcacc gacctcaatc cgggtagatc gtcgggtggt ggcgccgccg gcatcgcgtc ctggtggctg atgccgttct gcgcggtctg aaacgaagcc cccatgctga agatgacctc gccgtgctga acggcgttga cccgcctggt cacaaacgat ccgccatcgc gagtgcgctc gaccagaaaa accgtgggct cctgggcgtc cccggagcgc agaaagtacc cgtgcaacga gtggacctga tagcgcgggt cgaccgtgcg caccgccgac accagtgact gcccggccac atggccgccg aatgtgcgct gcagaaagcc tgattccggg ctgaagacac taccgcgata gatgttgacc tcgagttgct cgagatcaag aatctcctcg attgccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAIEEILD LEQLEVNIYR GSVFSPESGF LQRTFGGHVA GQSLVSAVRT VDPRYQVHSL HGYFLRSGDA QEPTVFLVER TRDGGSFVTR RVNAVQHGEV IFSMGASFQT AQNGISHQDA MPAAPPPDDL PGLRSVRVFD DAGFRQFEEW DVRIVPRDLL APLPGKASQQ QVWFRHRDPL PDDPVLHICA LAYMSDLTLL GSAQVTHLAE REHLQVASLD HAMWFMRGFR ADEWLLYDQS SPSAGGGRAL THGKIFTQGG ELVAAVMQEG LTRYPSGYQP GEN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCAATC GAGGAGATTC TTGATCTCGA GCAACTCGAG GTCAACATCT ATCGCGGTAG TGTCTTCAGC CCGGAATCAG GCTTTCTGCA GCGCACATTC GGCGGCCATG TGGCCGGGCA GTCACTGGTG TCGGCGGTGC GCACGGTCGA CCCGCGCTAT CAGGTCCACT CGTTGCACGG GTACTTTCTG CGCTCCGGGG ACGCCCAGGA GCCCACGGTT TTTCTGGTCG AGCGCACTCG CGATGGCGGA TCGTTTGTGA CCAGGCGGGT CAACGCCGTT CAGCACGGCG AGGTCATCTT CAGCATGGGG GCTTCGTTTC AGACCGCGCA GAACGGCATC AGCCACCAGG ACGCGATGCC GGCGGCGCCA CCACCCGACG ATCTACCCGG ATTGAGGTCG GTGCGGGTCT TCGACGACGC CGGGTTCCGG CAGTTCGAGG AGTGGGATGT GCGCATCGTG CCGCGGGACC TGCTCGCGCC GCTACCGGGT AAGGCATCCC AGCAGCAGGT GTGGTTTCGC CATCGCGACC CGCTGCCCGA TGACCCGGTG CTGCACATCT GTGCCTTGGC CTACATGAGC GATCTGACCC TGCTGGGTTC GGCGCAGGTC ACCCACCTGG CGGAGCGTGA GCATCTGCAG GTGGCGTCCC TGGACCACGC GATGTGGTTC ATGCGGGGGT TTCGGGCCGA CGAGTGGCTG CTCTACGACC AGTCATCGCC GTCGGCAGGC GGGGGTCGCG CACTTACGCA CGGCAAGATC TTCACCCAGG GAGGCGAGTT GGTGGCCGCG GTCATGCAGG AAGGGCTGAC CCGCTACCCA AGCGGATATC AGCCGGGCGA AAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA