MythA.18625.a

Ribosomal RNA small subunit methyltransferase G (EC 2.1.1.-) (16S rRNA 7-methylguanosine methyltransferase) (Rv3919c ortholog)

CENTER ID: MythA.18625.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.18625.a.B1.GE37656 full length 1 233
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.2105
RefSeq: WP_003925633.1
UniProt: G7CIN7

Orthologues for Rv3919c

Species % Identity % Coverage Clones Proteins Structures
marinum 77 98 1 0 0
ulcerans 75 72 0 0 0
paratuberculosis 73 97 1 0 0
avium 72 97 1 0 0
smegmatis 66 90 1 0 0
leprae 65 100 1 0 0
fortuitum 65 90 1 0 0
thermoresistibile 65 90 1 0 0
abscessus 60 97 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFHVKHGAVA APPPAAATIF GPALDRAQRY AAILAGAGVE RGLLGPREVD RIWDRHVLNS AVTSELIEPG ERVADIGSGA GLPGIPLALA RPDLSITLVE PMLRRSEFLH EVVAELDLEV RVVRGRAEDP AVRREVGEMD AVTSRAVAPL DKLARWSLPL LKPGGHMLAI KGARAEAEVD EHRRVLNRLK AVDVRVVRCG ADLLDPPATV VVARRAAAKQ GGYPPTRSGR RRG
NT Sequence
atgtttcacg tgaaacacgg tgcggtggcg gccccgcctc cggcggcggc gacgatcttc ggcccggcgc tggaccgggc gcagcggtac gcggccatcc tggccggagc gggtgtggag cgtgggttgt tgggtccgcg cgaagtggac cggatctggg atcggcacgt cctcaacagt gcggtgacca gcgagctgat cgagcccggt gagcgagtcg ccgatatcgg tagtggcgcc ggccttccgg ggataccgtt ggctctggcc cgtccggacc tcagtatcac cctcgtcgaa ccgatgctgc gccgaagtga attcctccac gaggtggtgg cggagctcga cctggaggtc cgggtggtgc ggggccgggc cgaggatccg gccgtacgcc gagaagtggg ggagatggac gccgtgacct cgcgggcggt cgccccgctg gacaagctcg cccggtggag cctgccgttg ctgaagccgg gcggccacat gctcgcgatc aagggcgccc gcgcggaggc ggaggtcgat gaacatcggc gtgtactgaa ccggttgaaa gcggtggatg tcagggtggt gagatgtggc gcggacttat tagatcctcc cgcgacggtg gtggtcgctc gccgcgcggc cgcgaagcag ggcggatatc cgccgacacg atcgggcagg agaaggggat ga
Details for MythA.18625.a.B1.GE37656
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 26kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF HVKHGAVAAP PPAAATIFGP ALDRAQRYAA ILAGAGVERG LLGPREVDRI WDRHVLNSAV TSELIEPGER VADIGSGAGL PGIPLALARP DLSITLVEPM LRRSEFLHEV VAELDLEVRV VRGRAEDPAV RREVGEMDAV TSRAVAPLDK LARWSLPLLK PGGHMLAIKG ARAEAEVDEH RRVLNRLKAV DVRVVRCGAD LLDPPATVVV ARRAAAKQGG YPPTRSGRRR G
Validated NT Sequence
tttnnctttn ggggggnntn tagcagccgg atcctatctt actcacttat ccccttctcc tgcccgatcg tgtcggcgga tatccgccct gcttcgcggc cgcgcggcga gcgaccacca ccgtcgcggg aggatctaat aagtccgcgc cacatctcac caccctgaca tccaccgctt tcaaccggtt cagtacacgc cgatgttcat cgacctccgc ctccgcgcgg gcgcccttga tcgcgagcat gtggccgccc ggcttcagca acggcaggct ccaccgggcg agcttgtcca gcggggcgac cgcccgcgag gtcacggcgt ccatctcccc cacttctcgg cgtacggccg gatcctcggc ccggccccgc accacccgga cctccaggtc gagctccgcc accacctcgt ggaggaattc acttcggcgc agcatcggtt cgacgagggt gatactgagg tccggacggg ccagagccaa cggtatcccc ggaaggccgg cgccactacc gatatcggcg actcgctcac cgggctcgat cagctcgctg gtcaccgcac tgttgaggac gtgccgatcc cagatccggt ccacttcgcg cggacccaac aacccacgct ccacacccgc tccggccagg atggccgcgt accgctgcgc ccggtccagc gccgggccga agatcgtcgc cgccgccgga ggcggggccg ccaccgcacc gtgtttcacg tgaaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMF HVKHGAVAAP PPAAATIFGP ALDRAQRYAA ILAGAGVERG LLGPREVDRI WDRHVLNSAV TSELIEPGER VADIGSGAGL PGIPLALARP DLSITLVEPM LRRSEFLHEV VAELDLEVRV VRGRAEDPAV RREVGEMDAV TSRAVAPLDK LARWSLPLLK PGGHMLAIKG ARAEAEVDEH RRVLNRLKAV DVRVVRCGAD LLDPPATVVV ARRAAAKQGG YPPTRSGRRR G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc acgtgaaaca cggtgcggtg gcggccccgc ctccggcggc ggcgacgatc ttcggcccgg cgctggaccg ggcgcagcgg tacgcggcca tcctggccgg agcgggtgtg gagcgtgggt tgttgggtcc gcgcgaagtg gaccggatct gggatcggca cgtcctcaac agtgcggtga ccagcgagct gatcgagccc ggtgagcgag tcgccgatat cggtagtggc gccggccttc cggggatacc gttggctctg gcccgtccgg acctcagtat caccctcgtc gaaccgatgc tgcgccgaag tgaattcctc cacgaggtgg tggcggagct cgacctggag gtccgggtgg tgcggggccg ggccgaggat ccggccgtac gccgagaagt gggggagatg gacgccgtga cctcgcgggc ggtcgccccg ctggacaagc tcgcccggtg gagcctgccg ttgctgaagc cgggcggcca catgctcgcg atcaagggcg cccgcgcgga ggcggaggtc gatgaacatc ggcgtgtact gaaccggttg aaagcggtgg atgtcagggt ggtgagatgt ggcgcggact tattagatcc tcccgcgacg gtggtggtcg ctcgccgcgc ggccgcgaag cagggcggat atccgccgac acgatcgggc aggagaaggg gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat