SSGCID: Target Page

MyabA.18625.a

Ribosomal RNA small subunit methyltransferase G (Rv3919c ortholog)

CENTER ID:	MyabA.18625.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.18625.a.B1.GE37331	full length		1	234

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.5033
RefSeq:	YP_001705673.1
UniProt:	B1MN93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHGETLPVP EAAAEVFGER LARAVEYVAA LATIGVERGL IGPRETGRLW GRHILNSAVL GELLEAGERV IDVGSGAGLP GIPLGIARPD VGIVLVEPML RRTEFLREMI EQLGLTNVSV VRGRAEEPAV IAEVGGADAV TSRAVAALDK IARWSLPLTR IGGRMLAIKG DRAEDEVEQY GSALGQLGAD DVRVVQCGTR YLDSPTTVVV ARRKECDGNR QRGSSRGGSR KRSR

NT Sequence

gtgaaacacg gcgagaccct cccggtgccg gaggccgctg ccgaggtgtt cggtgagcgg cttgcccggg cggtcgagta tgtagcggcg ctcgccacaa tcggcgtgga gcgaggtctg atcggacccc gcgagacggg tcgattgtgg gggcgccaca ttcttaacag cgctgtcctc ggggagctac tcgaggcggg ggagcgagtt attgacgtcg ggagcggcgc cgggctaccg ggaattcccc taggtatcgc gcggccagat gtcggtatcg ttctcgtcga gccgatgctt cggcgcacgg agtttctccg tgaaatgatc gagcagctgg ggctgaccaa tgtgtcggtg gtgcggggta gggcagagga gcccgctgtt atcgccgagg ttggcggggc ggacgcggtg acgtcccggg cggtcgcggc attggacaag atcgcccgct ggtctcttcc tttgacgcgg atcggcggac ggatgttggc gatcaaaggt gatcgtgcag aggatgaagt agagcagtac ggcagcgcgc tgggtcagct cggcgccgac gatgtgaggg tggtgcagtg tggcacgcgg tacctcgatt cgcccaccac ggtggtggtc gcgcggcgga aagaatgcga cggaaaccgg cagcggggaa gctcccgcgg cggttcgcgg aagcggagcc gatga

Details for MyabA.18625.a.B1.GE37331

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	26kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KHGETLPVPE AAAEVFGERL ARAVEYVAAL ATIGVERGLI GPRETGRLWG RHILNSAVLG ELLEAGERVI DVGSGAGLPG IPLGIARPDV GIVLVEPMLR RTEFLREMIE QLGLTNVSVV RGRAEEPAVI AEVGGADAVT SRAVAALDKI ARWSLPLTRI GGRMLAIKGD RAEDEVEQYG SALGQLGADD VRVVQCGTRY LDSPTTVVVA RRKECDGNRQ RGSSRGGSRK RSR

Validated NT Sequence

atcttactca cttatcggct ccgcttccgc gaaccgccgc gggagcttcc ccgctgccgg tttccgtcgc attctttccg ccgcgcgacc accaccgtgg tgggcgaatc gaggtaccgc gtgccacact gcaccaccct cacatcgtcg gcgccgagct gacccagcgc gctgccgtac tgctctactt catcctctgc acgatcacct ttgatcgcca acatccgtcc gccgatccgc gtcaaaggaa gagaccagcg ggcgatcttg tccaatgccg cgaccgcccg ggacgtcacc gcgtccgccc cgccaacctc ggcgataaca gcgggctcct ctgccctacc ccgcaccacc gacacattgg tcagccccag ctgctcgatc atttcacgga gaaactccgt gcgccgaagc atcggctcga cgagaacgat accgacatct ggccgcgcga tacctagggg aattcccggt agcccggcgc cgctcccgac gtcaataact cgctcccccg cctcgagtag ctccccgagg acagcgctgt taagaatgtg gcgcccccac aatcgacccg tctcgcgggg tccgatcaga cctcgctcca cgccgattgt ggcgagcgcc gctacatact cgaccgcccg ggcaagccgc tcaccgaaca cctcggcagc ggcctccggc accgggaggg tctcgccgtg tttcaccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMK HGETLPVPEA AAEVFGERLA RAVEYVAALA TIGVERGLIG PRETGRLWGR HILNSAVLGE LLEAGERVID VGSGAGLPGI PLGIARPDVG IVLVEPMLRR TEFLREMIEQ LGLTNVSVVR GRAEEPAVIA EVGGADAVTS RAVAALDKIA RWSLPLTRIG GRMLAIKGDR AEDEVEQYGS ALGQLGADDV RVVQCGTRYL DSPTTVVVAR RKECDGNRQR GSSRGGSRKR SR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaac acggcgagac cctcccggtg ccggaggccg ctgccgaggt gttcggtgag cggcttgccc gggcggtcga gtatgtagcg gcgctcgcca caatcggcgt ggagcgaggt ctgatcggac cccgcgagac gggtcgattg tgggggcgcc acattcttaa cagcgctgtc ctcggggagc tactcgaggc gggggagcga gttattgacg tcgggagcgg cgccgggcta ccgggaattc ccctaggtat cgcgcggcca gatgtcggta tcgttctcgt cgagccgatg cttcggcgca cggagtttct ccgtgaaatg atcgagcagc tggggctgac caatgtgtcg gtggtgcggg gtagggcaga ggagcccgct gttatcgccg aggttggcgg ggcggacgcg gtgacgtccc gggcggtcgc ggcattggac aagatcgccc gctggtctct tcctttgacg cggatcggcg gacggatgtt ggcgatcaaa ggtgatcgtg cagaggatga agtagagcag tacggcagcg cgctgggtca gctcggcgcc gacgatgtga gggtggtgca gtgtggcacg cggtacctcg attcgcccac cacggtggtg gtcgcgcggc ggaaagaatg cgacggaaac cggcagcggg gaagctcccg cggcggttcg cggaagcgga gccgatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat