MyleA.18625.a

Ribosomal RNA small subunit methyltransferase G (EC 2.1.1.-) (16S rRNA 7-methylguanosine methyltransferase) (16S rRNA m7G methyltransferase) (Rv3919c ortholog)

CENTER ID: MyleA.18625.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18625.a.B1.GE37465 full length 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.5233
RefSeq: YP_002504359.1
UniProt: B8ZTN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFHVKHVGSY EELASFTSVK GNLGFDTVFE AVFMIFGPRL NIAQRYVDLL ANTGIERGLL GPHEANRLWD RHLLNSAVVA ELLDPGDRVV DIGSGAGLPG LPLAIARPDL QVVLLEPLLR RVTFLREVVA ELGLDVEVVR GRAEELWVRD RIGERDVAVS RAVAALDKLT KWSIPLLRPG GQILAIKGEH VFDEIHQHRR VMASLGAVDV MVVVCGANYL CRPVTVVLTR CGQQMRHKPA RVGDRKTQ
NT Sequence
gtgtttcacg tgaaacatgt cggttcttac gaggagttgg catcctttac gtcagttaaa ggcaatcttg gatttgatac tgtgtttgaa gctgtattca tgatttttgg accacgtctt aatattgctc agcggtatgt cgatctactc gcaaatacag gaattgaacg tggattgctt ggtcctcatg aggctaaccg gctttgggat cggcatctat tgaacagtgc agtggtagcc gaacttcttg atcctggtga tcgggtagtg gatattggaa gtggtgcggg gttgccgggg ttgccgttgg ctattgcccg gccggatctt caggttgtgt tgcttgagcc actgttacgt cgagttactt ttcttcgaga agtagtggct gagcttggac ttgatgttga agtggttcgt ggccgagctg aggagttgtg ggtgcgtgat cggatcggtg aaagagatgt ggctgtatca cgagcggtag cggccttgga caagttgaca aagtggagca taccgttgtt acgaccaggt ggtcaaatac ttgcaatcaa aggggaacat gtttttgacg aaatccatca acatcggcgt gtaatggcct cattgggcgc cgttgatgtc atggtggtgg tatgtggtgc gaattatttg tgtcgtcccg taacagtagt cctaacgcga tgcggacagc agatgcggca caaaccagcg cgtgtggggg acaggaaaac acaatga
Details for MyleA.18625.a.B1.GE37465
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 28kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV FHVKHVGSYE ELASFTSVKG NLGFDTVFEA VFMIFGPRLN IAQRYVDLLA NTGIERGLLG PHEANRLWDR HLLNSAVVAE LLDPGDRVVD IGSGAGLPGL PLAIARPDLQ VVLLEPLLRR VTFLREVVAE LGLDVEVVRG RAEELWVRDR IGERDVAVSR AVAALDKLTK WSIPLLRPGG QILAIKGEHV FDEIHQHRRV MASLGAVDVM VVVCGANYLC RPVTVVLTRC GQQMRHKPAR VGDRKTQ
Validated NT Sequence
ttntttactt tanganggag anntaccatg gctcaccacc accaccacca tatggtgttt cacgtgaaac atgtcggttc ttacgaggag ttggcatcct ttacgtcagt taaaggcaat cttggatttg atactgtgtt tgaagctgta ttcatgattt ttggaccacg tcttaatatt gctcagcggt atgtcgatct actcgcaaat acaggaattg aacgtggatt gcttggtcct catgaggcta accggctttg ggatcggcat ctattgaaca gtgcagtggt agccgaactt cttgatcctg gtgatcgggt agtggatatt ggaagtggtg cggggttgcc ggggttgccg ttggctattg cccggccgga tcttcaggtt gtgttgcttg agccactgtt acgtcgagtt acttttcttc gagaagtagt ggctgagctt ggacttgatg ttgaagtggt tcgtggccga gctgaggagt tgtgggtgcg tgatcggatc ggtgaaagag atgtggctgt atcacgagcg gtagcggcct tggacaagtt gacaaagtgg agcataccgt tgttacgacc aggtggtcaa atacttgcaa tcaaagggga acatgttttt gacgaaatcc atcaacatcg gcgtgtaatg gcctcattgg gcgccgttga tgtcatggtg gtggtatgtg gtgcgaatta tttgtgtcgt cccgtaacag tagtcctaac gcgatgcgga cagcagatgc ggcacaaacc agcgcgtgtg ggggacagga aaacacaata agtgagtaan ataggatccn gctgctanan nccccccaan nnnannnnnn nnnnnnnnna nnnnnnnnnc nannccncan caaangnnnn ncntcnacnn nnnnnnnnnn nnccncnnna cccntnnncc nnnnncnnnn nnnnnnnntn nnncnnnnnn t
Expected Protein Sequence
MAHHHHHHMF HVKHVGSYEE LASFTSVKGN LGFDTVFEAV FMIFGPRLNI AQRYVDLLAN TGIERGLLGP HEANRLWDRH LLNSAVVAEL LDPGDRVVDI GSGAGLPGLP LAIARPDLQV VLLEPLLRRV TFLREVVAEL GLDVEVVRGR AEELWVRDRI GERDVAVSRA VAALDKLTKW SIPLLRPGGQ ILAIKGEHVF DEIHQHRRVM ASLGAVDVMV VVCGANYLCR PVTVVLTRCG QQMRHKPARV GDRKTQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtttc acgtgaaaca tgtcggttct tacgaggagt tggcatcctt tacgtcagtt aaaggcaatc ttggatttga tactgtgttt gaagctgtat tcatgatttt tggaccacgt cttaatattg ctcagcggta tgtcgatcta ctcgcaaata caggaattga acgtggattg cttggtcctc atgaggctaa ccggctttgg gatcggcatc tattgaacag tgcagtggta gccgaacttc ttgatcctgg tgatcgggta gtggatattg gaagtggtgc ggggttgccg gggttgccgt tggctattgc ccggccggat cttcaggttg tgttgcttga gccactgtta cgtcgagtta cttttcttcg agaagtagtg gctgagcttg gacttgatgt tgaagtggtt cgtggccgag ctgaggagtt gtgggtgcgt gatcggatcg gtgaaagaga tgtggctgta tcacgagcgg tagcggcctt ggacaagttg acaaagtgga gcataccgtt gttacgacca ggtggtcaaa tacttgcaat caaaggggaa catgtttttg acgaaatcca tcaacatcgg cgtgtaatgg cctcattggg cgccgttgat gtcatggtgg tggtatgtgg tgcgaattat ttgtgtcgtc ccgtaacagt agtcctaacg cgatgcggac agcagatgcg gcacaaacca gcgcgtgtgg gggacaggaa aacacaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at