SSGCID: Target Page

MypaA.18625.a

Ribosomal RNA small subunit methyltransferase G (Rv3919c ortholog)

CENTER ID:	MypaA.18625.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.18625.a.B1.GE37487	full length		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.4624
RefSeq:	NP_963279.1
UniProt:	Q9L7M3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFHVKHVGPV EPAAGDPEVP PVAELGAAPE SAAALFGPRL ATAQRYAEVL GTAGVERGLL GPREVDRIWD RHILNSAAVA ELLGRGDRII DIGSGAGLPG IPLAIARPDL EVVLLEPLLR RSEFLTEVVD ELGLAVEVVR GRAEERPVRD RFGERDAAVS RAVAALDKLT KWSMPLLRHD GRMLAIKGER AAEEVDRYRR VMTASGAADV RVVTCGANYL RPPATVVSAR RAKPPHPKSA RTGKAGTR

NT Sequence

atgtttcacg tgaaacatgt cggtccggtc gagccggctg cgggcgatcc ggaggtgcca cccgtcgcgg agctcggtgc cgcacccgag tccgccgcgg cgctgttcgg cccccgcctc gcgaccgcgc agcggtacgc ggaagtcctc ggcaccgcgg gtgtcgagcg gggcctgctc ggcccgcggg aagtcgaccg catctgggac cgtcacatcc tcaacagcgc ggcggtcgcc gaactcctgg gccgcggtga tcggatcatc gacatcggca gcggggcggg gctgcccggc atcccgttgg ccatcgcgcg acccgatctc gaggtggtat tgctggagcc gctcttgcgg cgcagcgagt ttctcaccga ggtggtggac gaactagggt tagccgtcga ggtggtccgc gggcgcgcgg aagagcggcc cgtgcgggac cggttcgggg agagggacgc ggcggtgtcg cgagcggttg cagcgttgga caagctgacg aagtggagta tgccgttgct acgccacgac ggccggatgc tcgccatcaa gggggagcgg gcggcggagg aagttgaccg ataccggcgt gtgatgacag catcgggcgc cgctgatgtc agggtggtga catgtggcgc gaactatttg cgtccccccg caaccgtagt ttcagctcga cgcgcaaagc cgccgcaccc caagtcggca cggaccggga aggccggaac ccgatga

Details for MypaA.18625.a.B1.GE37487

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF HVKHVGPVEP AAGDPEVPPV AELGAAPESA AALFGPRLAT AQRYAEVLGT AGVERGLLGP REVDRIWDRH ILNSAAVAEL LGRGDRIIDI GSGAGLPGIP LAIARPDLEV VLLEPLLRRS EFLTEVVDEL GLAVEVVRGR AEERPVRDRF GERDAAVSRA VAALDKLTKW SMPLLRHDGR MLAIKGERAA EEVDRYRRVM TASGAADVRV VTCGANYLRP PATVVSARRA KPPHPKSART GKAGTR

Validated NT Sequence

atcttactca cttatcgggt tccggccttc ccggtccgtg ccgacttggg gtgcggcggc tttgcgcgtc gagctgaaac tacggttgcg gggggacgca aatagttcgc gccacatgtc accaccctga catcagcggc gcccgatgct gtcatcacac gccggtatcg gtcaacttcc tccgccgccc gctccccctt gatggcgagc atccggccgt cgtggcgtag caacggcata ctccacttcg tcagcttgtc caacgctgca accgctcgcg acaccgccgc gtccctctcc ccgaaccggt cccgcacggg ccgctcttcc gcgcgcccgc ggaccacctc gacggctaac cctagttcgt ccaccacctc ggtgagaaac tcgctgcgcc gcaagagcgg ctccagcaat accacctcga gatcgggtcg cgcgatggcc aacgggatgc cgggcagccc cgccccgctg ccgatgtcga tgatccgatc accgcggccc aggagttcgg cgaccgccgc gctgttgagg atgtgacggt cccagatgcg gtcgacttcc cgcgggccga gcaggccccg ctcgacaccc gcggtgccga ggacttccgc gtaccgctgc gcggtcgcga ggcgggggcc gaacagcgcc gcggcggact cgggtgcggc accgagctcc gcgacgggtg gcacctccgg atcgcccgca gccggctcga ccggaccgac atgtttcacg tgaaacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc acgtgaaaca tgtcggtccg gtcgagccgg ctgcgggcga tccggaggtg ccacccgtcg cggagctcgg tgccgcaccc gagtccgccg cggcgctgtt cggcccccgc ctcgcgaccg cgcagcggta cgcggaagtc ctcggcaccg cgggtgtcga gcggggcctg ctcggcccgc gggaagtcga ccgcatctgg gaccgtcaca tcctcaacag cgcggcggtc gccgaactcc tgggccgcgg tgatcggatc atcgacatcg gcagcggggc ggggctgccc ggcatcccgt tggccatcgc gcgacccgat ctcgaggtgg tattgctgga gccgctcttg cggcgcagcg agtttctcac cgaggtggtg gacgaactag ggttagccgt cgaggtggtc cgcgggcgcg cggaagagcg gcccgtgcgg gaccggttcg gggagaggga cgcggcggtg tcgcgagcgg ttgcagcgtt ggacaagctg acgaagtgga gtatgccgtt gctacgccac gacggccgga tgctcgccat caagggggag cgggcggcgg aggaagttga ccgataccgg cgtgtgatga cagcatcggg cgccgctgat gtcagggtgg tgacatgtgg cgcgaactat ttgcgtcccc ccgcaaccgt agtttcagct cgacgcgcaa agccgccgca ccccaagtcg gcacggaccg ggaaggccgg aacccgatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at