SSGCID: Target Page

MyavA.18625.a

Ribosomal RNA small subunit methyltransferase G (Rv3919c ortholog)

CENTER ID:	MyavA.18625.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.18625.a.B1.GE37417	full length		1	245

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.5211
RefSeq:	YP_884418.1
UniProt:	A0QND0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHVGPVEPA AGGPEVPPVA ALGAAPESAA ALFGPRLATA QRYAEVLGTA GVERGLLGPR EVDRIWDRHI LNSAAVAGLL GRGDRIIDIG SGAGLPGIPL AIARPDLEVV LLEPLLRRSE FLTEVVDELG LAVEVVRGRA EERPVRNRFG DRDAAVSRAV AALDKLTKWS MPLLRHDGRM LAIKGERAAE EVDRYRRVMT ASGAADVRVV TCGANYLRPP ATVVSARRAK PPHPKSARTG KAGTR

NT Sequence

gtgaaacatg tcggtccggt cgagccggct gcgggcggtc cggaggtgcc acccgtcgcg gcgctcggtg ccgcacccga gtccgccgcg gcgctgttcg gcccccgcct cgcgaccgcg cagcggtacg cggaagtcct cggcaccgcg ggtgtcgagc gtggcctgct cggcccgcgg gaagtcgacc gcatctggga ccgccacatc ctcaacagcg cggcggtcgc cggactcctg ggccgcggtg atcggatcat cgacatcggc agcggggcgg ggctgcccgg catcccgttg gccatcgcgc gacccgatct cgaggtggta ttgctggagc cgctcttgcg gcgcagcgag tttctcaccg aggtggtgga cgaactaggg ttagccgtcg aggtggtccg cgggcgcgcg gaagagcggc ccgtgcggaa ccggttcggg gacagggacg cggcggtgtc gcgagcggtt gcagcgttgg acaagctgac gaagtggagt atgccgttgc tacgccacga cggccggatg ctcgccatca agggggagcg ggcggcggag gaagttgacc gataccggcg tgtgatgaca gcatcgggcg ccgctgatgt cagggtggtg acatgtggcg cgaactattt gcgtcccccc gcaaccgtag tttcagcgcg acgcgcaaag ccgccgcacc ccaagtcggc acggaccggg aaggccggaa cccgatga

Details for MyavA.18625.a.B1.GE37417

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KHVGPVEPAA GGPEVPPVAA LGAAPESAAA LFGPRLATAQ RYAEVLGTAG VERGLLGPRE VDRIWDRHIL NSAAVAGLLG RGDRIIDIGS GAGLPGIPLA IARPDLEVVL LEPLLRRSEF LTEVVDELGL AVEVVRGRAE ERPVRNRFGD RDAAVSRAVA ALDKLTKWSM PLLRHDGRML AIKGERAAEE VDRYRRVMTA SGAADVRVVT CGANYLRPPA TVVSARRAKP PHPKSARTGK AGTR

Validated NT Sequence

atcttactca cttatcgggt tccggccttc ccggtccgtg ccgacttggg gtgcggcggc tttgcgcgtc gcgctgaaac tacggttgcg gggggacgca aatagttcgc gccacatgtc accaccctga catcagcggc gcccgatgct gtcatcacac gccggtatcg gtcaacttcc tccgccgccc gctccccctt gatggcgagc atccggccgt cgtggcgtag caacggcata ctccacttcg tcagcttgtc caacgctgca accgctcgcg acaccgccgc gtccctgtcc ccgaaccggt tccgcacggg ccgctcttcc gcgcgcccgc ggaccacctc gacggctaac cctagttcgt ccaccacctc ggtgagaaac tcgctgcgcc gcaagagcgg ctccagcaat accacctcga gatcgggtcg cgcgatggcc aacgggatgc cgggcagccc cgccccgctg ccgatgtcga tgatccgatc accgcggccc aggagtccgg cgaccgccgc gctgttgagg atgtggcggt cccagatgcg gtcgacttcc cgcgggccga gcaggccacg ctcgacaccc gcggtgccga ggacttccgc gtaccgctgc gcggtcgcga ggcgggggcc gaacagcgcc gcggcggact cgggtgcggc accgagcgcc gcgacgggtg gcacctccgg accgcccgca gccggctcga ccggaccgac atgtttcacc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMK HVGPVEPAAG GPEVPPVAAL GAAPESAAAL FGPRLATAQR YAEVLGTAGV ERGLLGPREV DRIWDRHILN SAAVAGLLGR GDRIIDIGSG AGLPGIPLAI ARPDLEVVLL EPLLRRSEFL TEVVDELGLA VEVVRGRAEE RPVRNRFGDR DAAVSRAVAA LDKLTKWSMP LLRHDGRMLA IKGERAAEEV DRYRRVMTAS GAADVRVVTC GANYLRPPAT VVSARRAKPP HPKSARTGKA GTR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaac atgtcggtcc ggtcgagccg gctgcgggcg gtccggaggt gccacccgtc gcggcgctcg gtgccgcacc cgagtccgcc gcggcgctgt tcggcccccg cctcgcgacc gcgcagcggt acgcggaagt cctcggcacc gcgggtgtcg agcgtggcct gctcggcccg cgggaagtcg accgcatctg ggaccgccac atcctcaaca gcgcggcggt cgccggactc ctgggccgcg gtgatcggat catcgacatc ggcagcgggg cggggctgcc cggcatcccg ttggccatcg cgcgacccga tctcgaggtg gtattgctgg agccgctctt gcggcgcagc gagtttctca ccgaggtggt ggacgaacta gggttagccg tcgaggtggt ccgcgggcgc gcggaagagc ggcccgtgcg gaaccggttc ggggacaggg acgcggcggt gtcgcgagcg gttgcagcgt tggacaagct gacgaagtgg agtatgccgt tgctacgcca cgacggccgg atgctcgcca tcaaggggga gcgggcggcg gaggaagttg accgataccg gcgtgtgatg acagcatcgg gcgccgctga tgtcagggtg gtgacatgtg gcgcgaacta tttgcgtccc cccgcaaccg tagtttcagc gcgacgcgca aagccgccgc accccaagtc ggcacggacc gggaaggccg gaacccgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat