SSGCID: Target Page

MysmA.18625.a

Ribosomal RNA small subunit methyltransferase G (Rv3919c ortholog)

CENTER ID:	MysmA.18625.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.18625.a.B1.GE37631	full length		1	235

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.6753
RefSeq:	YP_891133.1
UniProt:	A0R7J5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHGSVPATP EAASLVFGDR LEAAELYARI LAGAGVEWGL LGPREVDRVW ERHILNSAAL GELMEPGERV ADIGSGAGLP GIPLALARPD IHVTLIEPLL RRSEFLRETV TELGLDVTVV RGRAEDREVR DRVGEMDVVT SRAVASLDKL TRWSVPFLRD GGRMLPIKGE RAEVEIEEHR RVMESLGAVD ARVVRCGANY LSPPVTVVDA RRRAAKPGRN KSGRTARSRG RTGRR

NT Sequence

gtgaaacatg gatccgttcc tgcgacccca gaggccgctt ctcttgtatt cggcgaccgg cttgaggcgg cggaactgta cgccaggatc ctcgccggcg ccggggtcga gtggggactc ctcggcccgc gcgaagtcga ccgggtgtgg gagcggcaca tcctcaacag cgcggctctc ggtgaactca tggagccggg ggagcgcgtc gccgacatcg gtagtggggc cggactcccc gggataccgt tggcgctggc gcgtcccgac atccacgtga cactgatcga gcccctcctc cggagaagcg agttcctccg cgagacggtg accgagctcg gcctcgacgt gacggtggtc cgtggacgtg ccgaggatcg agaggtacgc gaccgggtgg gggagatgga tgtggtgacg tcgcgcgcgg tcgcctcatt ggacaagctg acacggtgga gcgtgccgtt ccttcgggac ggaggccgga tgctcccgat caaaggcgag cgtgccgaag tcgagatcga ggaacaccgg cgtgtgatgg agtcactcgg agcggtggat gccagggtgg tgagatgtgg cgcgaactat ttgagcccgc ccgtaaccgt cgtcgacgca cggcggcggg cggccaaacc gggacggaac aagtcgggtc ggacggcgcg gtcccgggga agaacgggca ggagatga

Details for MysmA.18625.a.B1.GE37631

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	27kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KHGSVPATPE AASLVFGDRL EAAELYARIL AGAGVEWGLL GPREVDRVWE RHILNSAALG ELMEPGERVA DIGSGAGLPG IPLALARPDI HVTLIEPLLR RSEFLRETVT ELGLDVTVVR GRAEDREVRD RVGEMDVVTS RAVASLDKLT RWSVPFLRDG GRMLPIKGER AEVEIEEHRR VMESLGAVDA RVVRCGANYL SPPVTVVDAR RRAAKPGRNK SGRTARSRGR TGRR

Validated NT Sequence

atcttactca cttatctcct gcccgttctt ccccgggacc gcgccgtccg acccgacttg ttccgtcccg gtttggccgc ccgccgccgt gcgtcgacga cggttacggg cgggctcaaa tagttcgcgc cacatctcac caccctggca tccaccgctc cgagtgactc catcacacgc cggtgttcct cgatctcgac ttcggcacgc tcgcctttga tcgggagcat ccggcctccg tcccgaagga acggcacgct ccaccgtgtc agcttgtcca atgaggcgac cgcgcgcgac gtcaccacat ccatctcccc cacccggtcg cgtacctctc gatcctcggc acgtccacgg accaccgtca cgtcgaggcc gagctcggtc accgtctcgc ggaggaactc gcttctccgg aggaggggct cgatcagtgt cacgtggatg tcgggacgcg ccagcgccaa cggtatcccg gggagtccgg ccccactacc gatgtcggcg acgcgctccc ccggctccat gagttcaccg agagccgcgc tgttgaggat gtgccgctcc cacacccggt cgacttcgcg cgggccgagg agtccccact cgaccccggc gccggcgagg atcctggcgt acagttccgc cgcctcaagc cggtcgccga atacaagaga agcggcctct ggggtcgcag gaacggatcc atgtttcacc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMK HGSVPATPEA ASLVFGDRLE AAELYARILA GAGVEWGLLG PREVDRVWER HILNSAALGE LMEPGERVAD IGSGAGLPGI PLALARPDIH VTLIEPLLRR SEFLRETVTE LGLDVTVVRG RAEDREVRDR VGEMDVVTSR AVASLDKLTR WSVPFLRDGG RMLPIKGERA EVEIEEHRRV MESLGAVDAR VVRCGANYLS PPVTVVDARR RAAKPGRNKS GRTARSRGRT GRR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaac atggatccgt tcctgcgacc ccagaggccg cttctcttgt attcggcgac cggcttgagg cggcggaact gtacgccagg atcctcgccg gcgccggggt cgagtgggga ctcctcggcc cgcgcgaagt cgaccgggtg tgggagcggc acatcctcaa cagcgcggct ctcggtgaac tcatggagcc gggggagcgc gtcgccgaca tcggtagtgg ggccggactc cccgggatac cgttggcgct ggcgcgtccc gacatccacg tgacactgat cgagcccctc ctccggagaa gcgagttcct ccgcgagacg gtgaccgagc tcggcctcga cgtgacggtg gtccgtggac gtgccgagga tcgagaggta cgcgaccggg tgggggagat ggatgtggtg acgtcgcgcg cggtcgcctc attggacaag ctgacacggt ggagcgtgcc gttccttcgg gacggaggcc ggatgctccc gatcaaaggc gagcgtgccg aagtcgagat cgaggaacac cggcgtgtga tggagtcact cggagcggtg gatgccaggg tggtgagatg tggcgcgaac tatttgagcc cgcccgtaac cgtcgtcgac gcacggcggc gggcggccaa accgggacgg aacaagtcgg gtcggacggc gcggtcccgg ggaagaacgg gcaggagatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat