SSGCID: Target Page

MymaA.18625.a

Ribosomal RNA small subunit methyltransferase G (EC 2.1.1.-) (16S rRNA 7-methylguanosine methyltransferase) (16S rRNA m7G methyltransferase) (Rv3919c ortholog)

CENTER ID:	MymaA.18625.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18625.a.B1.GE37205	full length		1	228

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5878
RefSeq:	YP_001853744.1
UniProt:	B2HNT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQPLDDAAS AIFGPRLELA QRYADWLATA GVERGLLGPR EVDRLWERHV LNSAVIGELL DQGERVVDIG SGAGLPGLPL AIARPDLQVV LLEPMLRRVE FLQEVVTDLG LAVEVVRGRA EERSVRERLG GSDAAVSRAV AALDKLTKWS MPLLKREGRM LAIKGERAPD EVREHRRVME SLGAADVRVV PCGANYLQPP ATVVVARRGD TRGPNRRVSP RRTGGAPA

NT Sequence

atgacacagc ccctcgatga tgccgcgtcg gcgatcttcg gaccgcgcct cgagctcgcc caacgttatg ccgattggct ggccactgcc ggggtcgagc gggggttgct cggaccgcgt gaggtggacc ggctctggga acgtcacgtc ttgaactccg cggtgatcgg tgaacttctc gatcagggtg aacgcgtcgt agacatcgga agcggggcgg gtctgccggg cctaccgttg gcgatagccc gccccgacct ccaggtcgtc ttgttggagc caatgctccg gcgcgttgaa tttctgcagg aggtcgtcac agacctcggc ctggccgtgg aagtcgtgcg aggccgcgcg gaggaacggt cggtgcgaga gcgcctcggc ggcagtgatg ccgcggtttc gagagcggtg gccgcgttgg acaaactgac gaagtggagt atgccgttgt tgaagcggga ggggcgaatg ctggcgatca aaggggagcg agctcccgac gaagtgcgtg agcaccggcg tgtaatggag tcgttgggtg ccgcggatgt cagggtagtg ccatgtggcg cgaactactt gcaaccgccc gcaaccgtgg tggtagcccg acgcggggat acgcgtgggc ccaatcgacg ggtctctccg cggcgaacgg ggggagcacc ggcatga

Details for MymaA.18625.a.B1.GE37205

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT QPLDDAASAI FGPRLELAQR YADWLATAGV ERGLLGPREV DRLWERHVLN SAVIGELLDQ GERVVDIGSG AGLPGLPLAI ARPDLQVVLL EPMLRRVEFL QEVVTDLGLA VEVVRGRAEE RSVRERLGGS DAAVSRAVAA LDKLTKWSMP LLKREGRMLA IKGERAPDEV REHRRVMESL GAADVRVVPC GANYLQPPAT VVVARRGDTR GPNRRVSPRR TGGAPA

Validated NT Sequence

atcttactca cttatgccgg tgctcccccc gttcgccgcg gagagacccg tcgattgggc ccacgcgtat ccccgcgtcg ggctaccacc acggttgcgg gcggttgcaa gtagttcgcg ccacatggca ctaccctgac atccgcggca cccaacgact ccattacacg ccggtgctca cgcacttcgt cgggagctcg ctcccctttg atcgccagca ttcgcccctc ccgcttcaac aacggcatac tccacttcgt cagtttgtcc aacgcggcca ccgctctcga aaccgcggca tcactgccgc cgaggcgctc tcgcaccgac cgttcctccg cgcggcctcg cacgacttcc acggccaggc cgaggtctgt gacgacctcc tgcagaaatt caacgcgccg gagcattggc tccaacaaga cgacctggag gtcggggcgg gctatcgcca acggtaggcc cggcagaccc gccccgcttc cgatgtctac gacgcgttca ccctgatcga gaagttcacc gatcaccgcg gagttcaaga cgtgacgttc ccagagccgg tccacctcac gcggtccgag caacccccgc tcgaccccgg cagtggccag ccaatcggca taacgttggg cgagctcgag gcgcggtccg aagatcgccg acgcggcatc atcgaggggc tgtgtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac agcccctcga tgatgccgcg tcggcgatct tcggaccgcg cctcgagctc gcccaacgtt atgccgattg gctggccact gccggggtcg agcgggggtt gctcggaccg cgtgaggtgg accggctctg ggaacgtcac gtcttgaact ccgcggtgat cggtgaactt ctcgatcagg gtgaacgcgt cgtagacatc ggaagcgggg cgggtctgcc gggcctaccg ttggcgatag cccgccccga cctccaggtc gtcttgttgg agccaatgct ccggcgcgtt gaatttctgc aggaggtcgt cacagacctc ggcctggccg tggaagtcgt gcgaggccgc gcggaggaac ggtcggtgcg agagcgcctc ggcggcagtg atgccgcggt ttcgagagcg gtggccgcgt tggacaaact gacgaagtgg agtatgccgt tgttgaagcg ggaggggcga atgctggcga tcaaagggga gcgagctccc gacgaagtgc gtgagcaccg gcgtgtaatg gagtcgttgg gtgccgcgga tgtcagggta gtgccatgtg gcgcgaacta cttgcaaccg cccgcaaccg tggtggtagc ccgacgcggg gatacgcgtg ggcccaatcg acgggtctct ccgcggcgaa cggggggagc accggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at