MythA.18623.a
Glycoside hydrolase family protein (Rv0315 ortholog)
| CENTER ID: | MythA.18623.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.18623.a.B2.GE37655 | mature protein | 20 | 278 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.18623.a.B2.PS02026 | mature protein | 20 | 278 |
Structures
4XDQ
DEPOSITED: 12/19/2014
DETERMINATION: XRay
CLONE: MythA.18623.a.B2.GE37655
PROTEIN: MythA.18623.a.B2.PS02026
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.2044 |
| UniProt: | G7CI90 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMMLGFGAVA AALPLPQAGA QPVLDAPAPG PDTPEAASGP GLLFADEFDG PAGSPPDPAK WFIVPERETI RNPVEWDKPY NMGRYVTDQE HVFHDGNGNL VIRATRGPGA NIREKYASAK IVGLWRGGVG TTWEARVKLN CLTDGAWPAF WLLNDDPVRG AEIDIFEWYG NRDWPSGATV HAKLDGTMFQ TQNYPVDSAW HTWRMTWLPS GMYFWQDYEP GKEPFFTVLA NSLPEWPFND PGYTMVPVFN IAVGGSGGRE PAGGSYPADM IIDWIRVF
NT Sequence
atgatgatgc tgggattcgg tgcggtcgcc gccgcgctgc cgcttccgca ggccggcgcg cagccggtgc tcgatgcgcc ggctccggga ccggacacac cggaggcggc ctccgggcct ggtctgctgt tcgccgacga gttcgacggg ccggcggggt cgcccccgga tccggcgaag tggttcatcg ttccggagcg ggagacgatc cggaacccgg tcgaatggga caagccgtac aacatgggcc gctacgtcac cgaccaggag cacgtcttcc acgacggcaa cggcaacctg gtcatccgtg ccacccgcgg cccgggggcg aacatccggg agaagtacgc cagcgccaag atcgtcggcc tgtggcgtgg cggggtgggc accacctggg aggcacgcgt caaactcaac tgcctgaccg acggcgcctg gccggcgttc tggctgctca acgacgaccc ggtgcgcggc gccgagatcg acatcttcga gtggtacggc aaccgggact ggccgtcggg cgcaacggtg cacgccaaac tcgacggtac gatgttccag acccagaact acccggtgga cagtgcctgg cacacctggc ggatgacctg gctgccgtcg ggcatgtact tctggcagga ctacgagccg ggcaaggagc ccttcttcac cgtgctggcg aactcgttgc cggagtggcc gttcaacgat cccggttaca ccatggtgcc ggtcttcaac atcgccgtcg gagggtccgg cggccgtgag ccggccggag gcagctatcc ggcggacatg atcatcgact ggatccgggt gttctag
Details for MythA.18623.a.B2.GE37655
| HARVESTED ON: | 1/13/2014 |
| SEQUENCED ON: | 1/9/2014 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA QPVLDAPAPG PDTPEAASGP GLLFADEFDG PAGSPPDPAK WFIVPERETI RNPVEWDKPY NMGRYVTDQE HVFHDGNGNL VIRATRGPGA NIREKYASAK IVGLWRGGVG TTWEARVKLN CLTDGAWPAF WLLNDDPVRG AEIDIFEWYG NRDWPSGATV HAKLDGTMFQ TQNYPVDSAW HTWRMTWLPS GMYFWQDYEP GKEPFFTVLA NSLPEWPFND PGYTMVPVFN IAVGGSGGRE PAGGSYPADM IIDWIRVF
Validated NT Sequence
atcttactca cttagaacac ccggatccag tcgatgatca tgtccgccgg atagctgcct ccggccggct cacggccgcc ggaccctccg acggcgatgt tgaagaccgg caccatggtg taaccgggat cgttgaacgg ccactccggc aacgagttcg ccagcacggt gaagaagggc tccttgcccg gctcgtagtc ctgccagaag tacatgcccg acggcagcca ggtcatccgc caggtgtgcc aggcactgtc caccgggtag ttctgggtct ggaacatcgt accgtcgagt ttggcgtgca ccgttgcgcc cgacggccag tcccggttgc cgtaccactc gaagatgtcg atctcggcgc cgcgcaccgg gtcgtcgttg agcagccaga acgccggcca ggcgccgtcg gtcaggcagt tgagtttgac gcgtgcctcc caggtggtgc ccaccccgcc acgccacagg ccgacgatct tggcgctggc gtacttctcc cggatgttcg cccccgggcc gcgggtggca cggatgacca ggttgccgtt gccgtcgtgg aagacgtgct cctggtcggt gacgtagcgg cccatgttgt acggcttgtc ccattcgacc gggttccgga tcgtctcccg ctccggaacg atgaaccact tcgccggatc cgggggcgac cccgccggcc cgtcgaactc gtcggcgaac agcagaccag gcccggaggc cgcctccggt gtgtccggtc ccggagccgg cgcatcgagc accggctgcg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAQ PVLDAPAPGP DTPEAASGPG LLFADEFDGP AGSPPDPAKW FIVPERETIR NPVEWDKPYN MGRYVTDQEH VFHDGNGNLV IRATRGPGAN IREKYASAKI VGLWRGGVGT TWEARVKLNC LTDGAWPAFW LLNDDPVRGA EIDIFEWYGN RDWPSGATVH AKLDGTMFQT QNYPVDSAWH TWRMTWLPSG MYFWQDYEPG KEPFFTVLAN SLPEWPFNDP GYTMVPVFNI AVGGSGGREP AGGSYPADMI IDWIRVF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcagc cggtgctcga tgcgccggct ccgggaccgg acacaccgga ggcggcctcc gggcctggtc tgctgttcgc cgacgagttc gacgggccgg cggggtcgcc cccggatccg gcgaagtggt tcatcgttcc ggagcgggag acgatccgga acccggtcga atgggacaag ccgtacaaca tgggccgcta cgtcaccgac caggagcacg tcttccacga cggcaacggc aacctggtca tccgtgccac ccgcggcccg ggggcgaaca tccgggagaa gtacgccagc gccaagatcg tcggcctgtg gcgtggcggg gtgggcacca cctgggaggc acgcgtcaaa ctcaactgcc tgaccgacgg cgcctggccg gcgttctggc tgctcaacga cgacccggtg cgcggcgccg agatcgacat cttcgagtgg tacggcaacc gggactggcc gtcgggcgca acggtgcacg ccaaactcga cggtacgatg ttccagaccc agaactaccc ggtggacagt gcctggcaca cctggcggat gacctggctg ccgtcgggca tgtacttctg gcaggactac gagccgggca aggagccctt cttcaccgtg ctggcgaact cgttgccgga gtggccgttc aacgatcccg gttacaccat ggtgccggtc ttcaacatcg ccgtcggagg gtccggcggc cgtgagccgg ccggaggcag ctatccggcg gacatgatca tcgactggat ccgggtgttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MythA.18623.a.B2.PS02026
| PURIFICATION DATe: | 3/27/2014 |
| CONCENTRATION: | 20.8mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA QPVLDAPAPG PDTPEAASGP GLLFADEFDG PAGSPPDPAK WFIVPERETI RNPVEWDKPY NMGRYVTDQE HVFHDGNGNL VIRATRGPGA NIREKYASAK IVGLWRGGVG TTWEARVKLN CLTDGAWPAF WLLNDDPVRG AEIDIFEWYG NRDWPSGATV HAKLDGTMFQ TQNYPVDSAW HTWRMTWLPS GMYFWQDYEP GKEPFFTVLA NSLPEWPFND PGYTMVPVFN IAVGGSGGRE PAGGSYPADM IIDWIRVF
Validated NT Sequence
atcttactca cttagaacac ccggatccag tcgatgatca tgtccgccgg atagctgcct ccggccggct cacggccgcc ggaccctccg acggcgatgt tgaagaccgg caccatggtg taaccgggat cgttgaacgg ccactccggc aacgagttcg ccagcacggt gaagaagggc tccttgcccg gctcgtagtc ctgccagaag tacatgcccg acggcagcca ggtcatccgc caggtgtgcc aggcactgtc caccgggtag ttctgggtct ggaacatcgt accgtcgagt ttggcgtgca ccgttgcgcc cgacggccag tcccggttgc cgtaccactc gaagatgtcg atctcggcgc cgcgcaccgg gtcgtcgttg agcagccaga acgccggcca ggcgccgtcg gtcaggcagt tgagtttgac gcgtgcctcc caggtggtgc ccaccccgcc acgccacagg ccgacgatct tggcgctggc gtacttctcc cggatgttcg cccccgggcc gcgggtggca cggatgacca ggttgccgtt gccgtcgtgg aagacgtgct cctggtcggt gacgtagcgg cccatgttgt acggcttgtc ccattcgacc gggttccgga tcgtctcccg ctccggaacg atgaaccact tcgccggatc cgggggcgac cccgccggcc cgtcgaactc gtcggcgaac agcagaccag gcccggaggc cgcctccggt gtgtccggtc ccggagccgg cgcatcgagc accggctgcg ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHAQ PVLDAPAPGP DTPEAASGPG LLFADEFDGP AGSPPDPAKW FIVPERETIR NPVEWDKPYN MGRYVTDQEH VFHDGNGNLV IRATRGPGAN IREKYASAKI VGLWRGGVGT TWEARVKLNC LTDGAWPAFW LLNDDPVRGA EIDIFEWYGN RDWPSGATVH AKLDGTMFQT QNYPVDSAWH TWRMTWLPSG MYFWQDYEPG KEPFFTVLAN SLPEWPFNDP GYTMVPVFNI AVGGSGGREP AGGSYPADMI IDWIRVF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGCAGC CGGTGCTCGA TGCGCCGGCT CCGGGACCGG ACACACCGGA GGCGGCCTCC GGGCCTGGTC TGCTGTTCGC CGACGAGTTC GACGGGCCGG CGGGGTCGCC CCCGGATCCG GCGAAGTGGT TCATCGTTCC GGAGCGGGAG ACGATCCGGA ACCCGGTCGA ATGGGACAAG CCGTACAACA TGGGCCGCTA CGTCACCGAC CAGGAGCACG TCTTCCACGA CGGCAACGGC AACCTGGTCA TCCGTGCCAC CCGCGGCCCG GGGGCGAACA TCCGGGAGAA GTACGCCAGC GCCAAGATCG TCGGCCTGTG GCGTGGCGGG GTGGGCACCA CCTGGGAGGC ACGCGTCAAA CTCAACTGCC TGACCGACGG CGCCTGGCCG GCGTTCTGGC TGCTCAACGA CGACCCGGTG CGCGGCGCCG AGATCGACAT CTTCGAGTGG TACGGCAACC GGGACTGGCC GTCGGGCGCA ACGGTGCACG CCAAACTCGA CGGTACGATG TTCCAGACCC AGAACTACCC GGTGGACAGT GCCTGGCACA CCTGGCGGAT GACCTGGCTG CCGTCGGGCA TGTACTTCTG GCAGGACTAC GAGCCGGGCA AGGAGCCCTT CTTCACCGTG CTGGCGAACT CGTTGCCGGA GTGGCCGTTC AACGATCCCG GTTACACCAT GGTGCCGGTC TTCAACATCG CCGTCGGAGG GTCCGGCGGC CGTGAGCCGG CCGGAGGCAG CTATCCGGCG GACATGATCA TCGACTGGAT CCGGGTGTTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT