MymaA.18623.b
Beta-1,3-glucanase (Rv0315 ortholog)
| CENTER ID: | MymaA.18623.b |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.18623.b.B2.GE37203 | mature protein | 29 | 277 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.18623.b.B2.PS01925 | mature protein | 29 | 277 |
Structures
4PQ9
DEPOSITED: 2/28/2014
DETERMINATION: XRay
CLONE: MymaA.18623.b.B2.GE37203
PROTEIN: MymaA.18623.b.B2.PS01925
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216594.6.peg.3100 |
| RefSeq: | YP_001851197.1 |
| UniProt: | B2HE62 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEMDRRRMM LMTGFGVLGA ALPVPAASAD SSRRDMPLAP APNGQSGPYL FHDEFDGPAG SAPDSSKWTV ARAREEMKDP TYWERPENVG QYRDDRQNVF LDGKSNLVIR AAKDGGTYYA GKIQSPWRGG IGHTWEARIK FDCLTAGCWP AWWLGNQDRG EIDIIEWYGN GSWPSATTVH AKANGSEWKT RNVALDSGWH TWRCQWDETG MRFWQDYAEG AQPYFTVAAH SLPDWPFNDP GYTVFPVLNL AVAGSGGGDP RPGSYPAQML VDWVRVW
NT Sequence
atgcccgaga tggaccgtcg tcggatgatg ctgatgaccg gatttggagt gctcggggcg gcactgcccg tcccggcggc cagcgccgac tcgtctcgac gggacatgcc tctggcgccg gcgcccaatg gccaatcggg cccctacctg tttcacgacg aattcgatgg tccggccggc tctgcccccg actcgtcgaa gtggacggtg gcgcgagccc gcgaggagat gaaggacccg acgtactggg aacggcccga gaacgtcggc cagtaccgcg acgaccggca gaacgtcttc ctggacggta agtcgaatct ggtcatccga gccgcgaagg acggcggcac ctactacgcc ggcaagatcc aaagtccgtg gcggggcggc atcggccata cctgggaagc ccgtatcaaa ttcgactgtc tgaccgccgg ctgctggccg gcctggtggc tgggtaacca ggatcgcggc gaaatcgaca tcatcgagtg gtacggcaac gggagctggc cgtcggccac cactgtccac gcgaaagcaa acggttcgga gtggaagacc cgcaacgtcg cgttggacag cgggtggcac acctggcgat gccaatggga cgagaccggt atgcggttct ggcaggacta cgccgagggg gcgcagccct acttcaccgt cgcggcgcat tcgctacccg actggccgtt caacgatccc ggctacaccg tgttccccgt cctcaacctc gcggttgccg gatccggcgg cggggatccc agaccgggca gctatccggc acagatgctg gtcgactggg tacgcgtctg gtga
Details for MymaA.18623.b.B2.GE37203
| HARVESTED ON: | 8/13/2013 |
| SEQUENCED ON: | 8/15/2013 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA DSSRRDMPLA PAPNGQSGPY LFHDEFDGPA GSAPDSSKWT VARAREEMKD PTYWERPENV GQYRDDRQNV FLDGKSNLVI RAAKDGGTYY AGKIQSPWRG GIGHTWEARI KFDCLTAGCW PAWWLGNQDR GEIDIIEWYG NGSWPSATTV HAKANGSEWK TRNVALDSGW HTWRCQWDET GMRFWQDYAE GAQPYFTVAA HSLPDWPFND PGYTVFPVLN LAVAGSGGGD PRPGSYPAQM LVDWVRVW
Validated NT Sequence
atcttactca cttaccagac gcgtacccag tcgaccagca tctgtgccgg atagctgccc ggtctgggat ccccgccgcc ggatccggca accgcgaggt tgaggacggg gaacacggtg tagccgggat cgttgaacgg ccagtcgggt agcgaatgcg ccgcgacggt gaagtagggc tgcgccccct cggcgtagtc ctgccagaac cgcataccgg tctcgtccca ttggcatcgc caggtgtgcc acccgctgtc caacgcgacg ttgcgggtct tccactccga accgtttgct ttcgcgtgga cagtggtggc cgacggccag ctcccgttgc cgtaccactc gatgatgtcg atttcgccgc gatcctggtt acccagccac caggccggcc agcagccggc ggtcagacag tcgaatttga tacgggcttc ccaggtatgg ccgatgccgc cccgccacgg actttggatc ttgccggcgt agtaggtgcc gccgtccttc gcggctcgga tgaccagatt cgacttaccg tccaggaaga cgttctgccg gtcgtcgcgg tactggccga cgttctcggg ccgttcccag tacgtcgggt ccttcatctc ctcgcgggct cgcgccaccg tccacttcga cgagtcgggg gcagagccgg ccggaccatc gaattcgtcg tgaaacaggt aggggcccga ttggccattg ggcgccggcg ccagaggcat gtcccgtcga gacgagtcgg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAD SSRRDMPLAP APNGQSGPYL FHDEFDGPAG SAPDSSKWTV ARAREEMKDP TYWERPENVG QYRDDRQNVF LDGKSNLVIR AAKDGGTYYA GKIQSPWRGG IGHTWEARIK FDCLTAGCWP AWWLGNQDRG EIDIIEWYGN GSWPSATTVH AKANGSEWKT RNVALDSGWH TWRCQWDETG MRFWQDYAEG AQPYFTVAAH SLPDWPFNDP GYTVFPVLNL AVAGSGGGDP RPGSYPAQML VDWVRVW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgact cgtctcgacg ggacatgcct ctggcgccgg cgcccaatgg ccaatcgggc ccctacctgt ttcacgacga attcgatggt ccggccggct ctgcccccga ctcgtcgaag tggacggtgg cgcgagcccg cgaggagatg aaggacccga cgtactggga acggcccgag aacgtcggcc agtaccgcga cgaccggcag aacgtcttcc tggacggtaa gtcgaatctg gtcatccgag ccgcgaagga cggcggcacc tactacgccg gcaagatcca aagtccgtgg cggggcggca tcggccatac ctgggaagcc cgtatcaaat tcgactgtct gaccgccggc tgctggccgg cctggtggct gggtaaccag gatcgcggcg aaatcgacat catcgagtgg tacggcaacg ggagctggcc gtcggccacc actgtccacg cgaaagcaaa cggttcggag tggaagaccc gcaacgtcgc gttggacagc gggtggcaca cctggcgatg ccaatgggac gagaccggta tgcggttctg gcaggactac gccgaggggg cgcagcccta cttcaccgtc gcggcgcatt cgctacccga ctggccgttc aacgatcccg gctacaccgt gttccccgtc ctcaacctcg cggttgccgg atccggcggc ggggatccca gaccgggcag ctatccggca cagatgctgg tcgactgggt acgcgtctgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MymaA.18623.b.B2.PS01925
| PURIFICATION DATe: | 10/29/2013 |
| CONCENTRATION: | 27mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DSSRRDMPLA PAPNGQSGPY LFHDEFDGPA GSAPDSSKWT VARAREEMKD PTYWERPENV GQYRDDRQNV FLDGKSNLVI RAAKDGGTYY AGKIQSPWRG GIGHTWEARI KFDCLTAGCW PAWWLGNQDR GEIDIIEWYG NGSWPSATTV HAKANGSEWK TRNVALDSGW HTWRCQWDET GMRFWQDYAE GAQPYFTVAA HSLPDWPFND PGYTVFPVLN LAVAGSGGGD PRPGSYPAQM LVDWVRVW
Validated NT Sequence
atcttactca cttaccagac gcgtacccag tcgaccagca tctgtgccgg atagctgccc ggtctgggat ccccgccgcc ggatccggca accgcgaggt tgaggacggg gaacacggtg tagccgggat cgttgaacgg ccagtcgggt agcgaatgcg ccgcgacggt gaagtagggc tgcgccccct cggcgtagtc ctgccagaac cgcataccgg tctcgtccca ttggcatcgc caggtgtgcc acccgctgtc caacgcgacg ttgcgggtct tccactccga accgtttgct ttcgcgtgga cagtggtggc cgacggccag ctcccgttgc cgtaccactc gatgatgtcg atttcgccgc gatcctggtt acccagccac caggccggcc agcagccggc ggtcagacag tcgaatttga tacgggcttc ccaggtatgg ccgatgccgc cccgccacgg actttggatc ttgccggcgt agtaggtgcc gccgtccttc gcggctcgga tgaccagatt cgacttaccg tccaggaaga cgttctgccg gtcgtcgcgg tactggccga cgttctcggg ccgttcccag tacgtcgggt ccttcatctc ctcgcgggct cgcgccaccg tccacttcga cgagtcgggg gcagagccgg ccggaccatc gaattcgtcg tgaaacaggt aggggcccga ttggccattg ggcgccggcg ccagaggcat gtcccgtcga gacgagtcgg ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHAD SSRRDMPLAP APNGQSGPYL FHDEFDGPAG SAPDSSKWTV ARAREEMKDP TYWERPENVG QYRDDRQNVF LDGKSNLVIR AAKDGGTYYA GKIQSPWRGG IGHTWEARIK FDCLTAGCWP AWWLGNQDRG EIDIIEWYGN GSWPSATTVH AKANGSEWKT RNVALDSGWH TWRCQWDETG MRFWQDYAEG AQPYFTVAAH SLPDWPFNDP GYTVFPVLNL AVAGSGGGDP RPGSYPAQML VDWVRVW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGACT CGTCTCGACG GGACATGCCT CTGGCGCCGG CGCCCAATGG CCAATCGGGC CCCTACCTGT TTCACGACGA ATTCGATGGT CCGGCCGGCT CTGCCCCCGA CTCGTCGAAG TGGACGGTGG CGCGAGCCCG CGAGGAGATG AAGGACCCGA CGTACTGGGA ACGGCCCGAG AACGTCGGCC AGTACCGCGA CGACCGGCAG AACGTCTTCC TGGACGGTAA GTCGAATCTG GTCATCCGAG CCGCGAAGGA CGGCGGCACC TACTACGCCG GCAAGATCCA AAGTCCGTGG CGGGGCGGCA TCGGCCATAC CTGGGAAGCC CGTATCAAAT TCGACTGTCT GACCGCCGGC TGCTGGCCGG CCTGGTGGCT GGGTAACCAG GATCGCGGCG AAATCGACAT CATCGAGTGG TACGGCAACG GGAGCTGGCC GTCGGCCACC ACTGTCCACG CGAAAGCAAA CGGTTCGGAG TGGAAGACCC GCAACGTCGC GTTGGACAGC GGGTGGCACA CCTGGCGATG CCAATGGGAC GAGACCGGTA TGCGGTTCTG GCAGGACTAC GCCGAGGGGG CGCAGCCCTA CTTCACCGTC GCGGCGCATT CGCTACCCGA CTGGCCGTTC AACGATCCCG GCTACACCGT GTTCCCCGTC CTCAACCTCG CGGTTGCCGG ATCCGGCGGC GGGGATCCCA GACCGGGCAG CTATCCGGCA CAGATGCTGG TCGACTGGGT ACGCGTCTGG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT