MypaA.18623.a
Putative uncharacterized protein (Rv0315 ortholog)
| CENTER ID: | MypaA.18623.a |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.18623.a.B2.GE37484 | mature protein | 31 | 275 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.18623.a.B2.PS01983 | mature protein | 31 | 275 |
Structures
4XXP
DEPOSITED: 1/30/2015
DETERMINATION: XRay
CLONE: MypaA.18623.a.B2.GE37484
PROTEIN: MypaA.18623.a.B2.PS01983
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|262316.17.peg.1754 |
| RefSeq: | NP_960595.1 |
| UniProt: | Q73ZE2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLMPEIDRRR MIVTTGIGVL AAALPNPRAG ASPAPPPAAP SGQSGTYLFQ DEFDGPAGSA PDASKWAVAK ARETIKDPTY WERPENIGQY RDDRRNVFLD GKSNLVLRAA KNGPTYYSGK VQSLWRGGVG HTWEARIKLD CLTAGAWPAY WLGNDDQGEI DVMEWYGNGN WPSATTVHAK ANGGEWKTHN IAVDSAWHTW RCQWDEAGIR FWKDYVDGAQ PYFDVPASSL PDWPFNGPGY TVFPVFNLAV AGSGGGDPGP GSYPADMLID WIRVW
NT Sequence
ttgcttatgc cggagatcga tcgccgccgc atgattgtga ccaccggaat cggcgtgctc gcagcggcgc tgccgaaccc gagagccggg gccagtccgg ccccaccgcc cgcggcgccc agcggccaat ccgggaccta cctcttccag gatgagttcg acggtccggc cggctcggcg cccgacgcgt ccaaatgggc ggtggccaaa gcccgcgaga cgatcaagga tccgacctac tgggaacgcc cggagaacat cgggcagtac cgcgacgacc gccgcaacgt cttcctcgac ggcaagtcca acctggtgct gcgcgccgcc aagaacggcc ccacctacta cagcggcaag gtgcagagcc tgtggcgcgg cggcgtcggc cacacctggg aggcccggat caaactggac tgcctgaccg ccggcgcctg gccggcttac tggctgggca atgacgacca gggcgaaatc gacgtcatgg agtggtacgg caacggcaac tggccctcgg ccaccaccgt gcatgccaaa gccaacggcg gcgagtggaa gacccacaac atcgcggtgg acagcgcctg gcacacctgg cgatgccagt gggacgaggc cggcatccgg ttctggaagg actacgtcga cggcgcgcaa ccgtacttcg acgtgcccgc cagttcgctg ccggattggc cgttcaacgg cccgggctac acggtttttc cggtgttcaa cctggcggtg gccgggtccg gcggcggcga ccccggcccg ggcagctatc ccgcggacat gctcatcgac tggatccgcg tctggtag
Details for MypaA.18623.a.B2.GE37484
| HARVESTED ON: | 10/31/2013 |
| SEQUENCED ON: | 11/5/2013 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA SPAPPPAAPS GQSGTYLFQD EFDGPAGSAP DASKWAVAKA RETIKDPTYW ERPENIGQYR DDRRNVFLDG KSNLVLRAAK NGPTYYSGKV QSLWRGGVGH TWEARIKLDC LTAGAWPAYW LGNDDQGEID VMEWYGNGNW PSATTVHAKA NGGEWKTHNI AVDSAWHTWR CQWDEAGIRF WKDYVDGAQP YFDVPASSLP DWPFNGPGYT VFPVFNLAVA GSGGGDPGPG SYPADMLIDW IRVW
Validated NT Sequence
atcttactca cttaccagac gcggatccag tcgatgagca tgtccgcggg atagctgccc gggccggggt cgccgccgcc ggacccggcc accgccaggt tgaacaccgg aaaaaccgtg tagcccgggc cgttgaacgg ccaatccggc agcgaactgg cgggcacgtc gaagtacggt tgcgcgccgt cgacgtagtc cttccagaac cggatgccgg cctcgtccca ctggcatcgc caggtgtgcc aggcgctgtc caccgcgatg ttgtgggtct tccactcgcc gccgttggct ttggcatgca cggtggtggc cgagggccag ttgccgttgc cgtaccactc catgacgtcg atttcgccct ggtcgtcatt gcccagccag taagccggcc aggcgccggc ggtcaggcag tccagtttga tccgggcctc ccaggtgtgg ccgacgccgc cgcgccacag gctctgcacc ttgccgctgt agtaggtggg gccgttcttg gcggcgcgca gcaccaggtt ggacttgccg tcgaggaaga cgttgcggcg gtcgtcgcgg tactgcccga tgttctccgg gcgttcccag taggtcggat ccttgatcgt ctcgcgggct ttggccaccg cccatttgga cgcgtcgggc gccgagccgg ccggaccgtc gaactcatcc tggaagaggt aggtcccgga ttggccgctg ggcgccgcgg gcggtggggc cggactggcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHAS PAPPPAAPSG QSGTYLFQDE FDGPAGSAPD ASKWAVAKAR ETIKDPTYWE RPENIGQYRD DRRNVFLDGK SNLVLRAAKN GPTYYSGKVQ SLWRGGVGHT WEARIKLDCL TAGAWPAYWL GNDDQGEIDV MEWYGNGNWP SATTVHAKAN GGEWKTHNIA VDSAWHTWRC QWDEAGIRFW KDYVDGAQPY FDVPASSLPD WPFNGPGYTV FPVFNLAVAG SGGGDPGPGS YPADMLIDWI RVW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccagtc cggccccacc gcccgcggcg cccagcggcc aatccgggac ctacctcttc caggatgagt tcgacggtcc ggccggctcg gcgcccgacg cgtccaaatg ggcggtggcc aaagcccgcg agacgatcaa ggatccgacc tactgggaac gcccggagaa catcgggcag taccgcgacg accgccgcaa cgtcttcctc gacggcaagt ccaacctggt gctgcgcgcc gccaagaacg gccccaccta ctacagcggc aaggtgcaga gcctgtggcg cggcggcgtc ggccacacct gggaggcccg gatcaaactg gactgcctga ccgccggcgc ctggccggct tactggctgg gcaatgacga ccagggcgaa atcgacgtca tggagtggta cggcaacggc aactggccct cggccaccac cgtgcatgcc aaagccaacg gcggcgagtg gaagacccac aacatcgcgg tggacagcgc ctggcacacc tggcgatgcc agtgggacga ggccggcatc cggttctgga aggactacgt cgacggcgcg caaccgtact tcgacgtgcc cgccagttcg ctgccggatt ggccgttcaa cggcccgggc tacacggttt ttccggtgtt caacctggcg gtggccgggt ccggcggcgg cgaccccggc ccgggcagct atcccgcgga catgctcatc gactggatcc gcgtctggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MypaA.18623.a.B2.PS01983
| PURIFICATION DATe: | 1/16/2014 |
| CONCENTRATION: | 26.3mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA SPAPPPAAPS GQSGTYLFQD EFDGPAGSAP DASKWAVAKA RETIKDPTYW ERPENIGQYR DDRRNVFLDG KSNLVLRAAK NGPTYYSGKV QSLWRGGVGH TWEARIKLDC LTAGAWPAYW LGNDDQGEID VMEWYGNGNW PSATTVHAKA NGGEWKTHNI AVDSAWHTWR CQWDEAGIRF WKDYVDGAQP YFDVPASSLP DWPFNGPGYT VFPVFNLAVA GSGGGDPGPG SYPADMLIDW IRVW
Validated NT Sequence
atcttactca cttaccagac gcggatccag tcgatgagca tgtccgcggg atagctgccc gggccggggt cgccgccgcc ggacccggcc accgccaggt tgaacaccgg aaaaaccgtg tagcccgggc cgttgaacgg ccaatccggc agcgaactgg cgggcacgtc gaagtacggt tgcgcgccgt cgacgtagtc cttccagaac cggatgccgg cctcgtccca ctggcatcgc caggtgtgcc aggcgctgtc caccgcgatg ttgtgggtct tccactcgcc gccgttggct ttggcatgca cggtggtggc cgagggccag ttgccgttgc cgtaccactc catgacgtcg atttcgccct ggtcgtcatt gcccagccag taagccggcc aggcgccggc ggtcaggcag tccagtttga tccgggcctc ccaggtgtgg ccgacgccgc cgcgccacag gctctgcacc ttgccgctgt agtaggtggg gccgttcttg gcggcgcgca gcaccaggtt ggacttgccg tcgaggaaga cgttgcggcg gtcgtcgcgg tactgcccga tgttctccgg gcgttcccag taggtcggat ccttgatcgt ctcgcgggct ttggccaccg cccatttgga cgcgtcgggc gccgagccgg ccggaccgtc gaactcatcc tggaagaggt aggtcccgga ttggccgctg ggcgccgcgg gcggtggggc cggactggcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHAS PAPPPAAPSG QSGTYLFQDE FDGPAGSAPD ASKWAVAKAR ETIKDPTYWE RPENIGQYRD DRRNVFLDGK SNLVLRAAKN GPTYYSGKVQ SLWRGGVGHT WEARIKLDCL TAGAWPAYWL GNDDQGEIDV MEWYGNGNWP SATTVHAKAN GGEWKTHNIA VDSAWHTWRC QWDEAGIRFW KDYVDGAQPY FDVPASSLPD WPFNGPGYTV FPVFNLAVAG SGGGDPGPGS YPADMLIDWI RVW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCAGTC CGGCCCCACC GCCCGCGGCG CCCAGCGGCC AATCCGGGAC CTACCTCTTC CAGGATGAGT TCGACGGTCC GGCCGGCTCG GCGCCCGACG CGTCCAAATG GGCGGTGGCC AAAGCCCGCG AGACGATCAA GGATCCGACC TACTGGGAAC GCCCGGAGAA CATCGGGCAG TACCGCGACG ACCGCCGCAA CGTCTTCCTC GACGGCAAGT CCAACCTGGT GCTGCGCGCC GCCAAGAACG GCCCCACCTA CTACAGCGGC AAGGTGCAGA GCCTGTGGCG CGGCGGCGTC GGCCACACCT GGGAGGCCCG GATCAAACTG GACTGCCTGA CCGCCGGCGC CTGGCCGGCT TACTGGCTGG GCAATGACGA CCAGGGCGAA ATCGACGTCA TGGAGTGGTA CGGCAACGGC AACTGGCCCT CGGCCACCAC CGTGCATGCC AAAGCCAACG GCGGCGAGTG GAAGACCCAC AACATCGCGG TGGACAGCGC CTGGCACACC TGGCGATGCC AGTGGGACGA GGCCGGCATC CGGTTCTGGA AGGACTACGT CGACGGCGCG CAACCGTACT TCGACGTGCC CGCCAGTTCG CTGCCGGATT GGCCGTTCAA CGGCCCGGGC TACACGGTTT TTCCGGTGTT CAACCTGGCG GTGGCCGGGT CCGGCGGCGG CGACCCCGGC CCGGGCAGCT ATCCCGCGGA CATGCTCATC GACTGGATCC GCGTCTGGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT