MysmA.18623.a

Putative beta-1,3-glucanase (Rv0315 ortholog)

CENTER ID: MysmA.18623.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18623.a.B2.GE37630 mature protein 21 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18623.a.B2.PS02010 mature protein 21 280
MysmA.18623.a.B2.PS02011 mature protein 21 280

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.641
RefSeq: YP_885055.1
UniProt: A0QQ67

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMFMMGLGVA AAALPVGTAG ADPLIGDNPP GPGPAAAAEP TFLFQDEFNG PAGSPPDPAW WYLVPARETI KNPVEWDKPY NMGRYVTDQE HAFQDGKGNL VIRATRGPGT TIQEKYASAK VVGLWRGGIG TTWEARVKLN CLTDGAWPAF WLLNDNPVRG GEVDLVEWYG NRDWPSGTTV HARLDGTSFA TNPHPVDSDW HTWRMSWKPE GMYFWKDYAP GMEPFFSVPA NSLQDWPFND PGYTMVPVFN IAVGGSGGRE PAGGTYPAEM LVDWIRVFAG
NT Sequence
gtgatgttca tgatgggtct gggggtggca gccgctgcac ttcccgtcgg gacggccggc gccgacccac tcatcggaga taacccgccc ggtcccgggc ccgccgcggc agccgagccc accttcctgt tccaggacga gttcaacggt cccgcgggat cgccgccgga tcccgcgtgg tggtacctgg tgcccgcccg cgagaccatc aagaaccccg tcgaatggga caagccgtac aacatggggc ggtatgtcac cgaccaggaa catgccttcc aggacggcaa gggcaatctg gtcatccgcg cgacgcgggg gccgggtacc accatccagg agaagtacgc cagtgccaag gtggtcggcc tgtggcgcgg tggcatcggc accacctggg aagcccgcgt caagctgaac tgcctgaccg acggtgcgtg gcccgcgttc tggcttctca acgacaaccc cgtgcgtggc ggcgaggtcg acctcgtcga gtggtacggc aaccgagact ggccgtcggg caccacggtg cacgcccgcc tcgacggcac gtcgttcgcg accaatccgc atcccgtcga cagcgactgg cacacctggc gcatgtcgtg gaaacccgaa ggcatgtact tctggaagga ctacgcgccg ggtatggagc cgttcttctc ggttcccgcg aactcgttgc aggactggcc tttcaacgat cccggttaca ccatggtccc ggtgttcaac atcgccgtcg gcggctccgg cggacgtgaa cccgccggag gcacctatcc ggccgagatg ctggtcgact ggatccgggt gttcgccggc tga
Details for MysmA.18623.a.B2.GE37630
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 30kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA DPLIGDNPPG PGPAAAAEPT FLFQDEFNGP AGSPPDPAWW YLVPARETIK NPVEWDKPYN MGRYVTDQEH AFQDGKGNLV IRATRGPGTT IQEKYASAKV VGLWRGGIGT TWEARVKLNC LTDGAWPAFW LLNDNPVRGG EVDLVEWYGN RDWPSGTTVH ARLDGTSFAT NPHPVDSDWH TWRMSWKPEG MYFWKDYAPG MEPFFSVPAN SLQDWPFNDP GYTMVPVFNI AVGGSGGREP AGGTYPAEML VDWIRVFAG
Validated NT Sequence
atcttactca cttagccggc gaacacccgg atccagtcga ccagcatctc ggccggatag gtgcctccgg cgggttcacg tccgccggag ccgccgacgg cgatgttgaa caccgggacc atggtgtaac cgggatcgtt gaaaggccag tcctgcaacg agttcgcggg aaccgagaag aacggctcca tacccggcgc gtagtccttc cagaagtaca tgccttcggg tttccacgac atgcgccagg tgtgccagtc gctgtcgacg ggatgcggat tggtcgcgaa cgacgtgccg tcgaggcggg cgtgcaccgt ggtgcccgac ggccagtctc ggttgccgta ccactcgacg aggtcgacct cgccgccacg cacggggttg tcgttgagaa gccagaacgc gggccacgca ccgtcggtca ggcagttcag cttgacgcgg gcttcccagg tggtgccgat gccaccgcgc cacaggccga ccaccttggc actggcgtac ttctcctgga tggtggtacc cggcccccgc gtcgcgcgga tgaccagatt gcccttgccg tcctggaagg catgttcctg gtcggtgaca taccgcccca tgttgtacgg cttgtcccat tcgacggggt tcttgatggt ctcgcgggcg ggcaccaggt accaccacgc gggatccggc ggcgatcccg cgggaccgtt gaactcgtcc tggaacagga aggtgggctc ggctgccgcg gcgggcccgg gaccgggcgg gttatctccg atgagtgggt cggccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHAD PLIGDNPPGP GPAAAAEPTF LFQDEFNGPA GSPPDPAWWY LVPARETIKN PVEWDKPYNM GRYVTDQEHA FQDGKGNLVI RATRGPGTTI QEKYASAKVV GLWRGGIGTT WEARVKLNCL TDGAWPAFWL LNDNPVRGGE VDLVEWYGNR DWPSGTTVHA RLDGTSFATN PHPVDSDWHT WRMSWKPEGM YFWKDYAPGM EPFFSVPANS LQDWPFNDPG YTMVPVFNIA VGGSGGREPA GGTYPAEMLV DWIRVFAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgacc cactcatcgg agataacccg cccggtcccg ggcccgccgc ggcagccgag cccaccttcc tgttccagga cgagttcaac ggtcccgcgg gatcgccgcc ggatcccgcg tggtggtacc tggtgcccgc ccgcgagacc atcaagaacc ccgtcgaatg ggacaagccg tacaacatgg ggcggtatgt caccgaccag gaacatgcct tccaggacgg caagggcaat ctggtcatcc gcgcgacgcg ggggccgggt accaccatcc aggagaagta cgccagtgcc aaggtggtcg gcctgtggcg cggtggcatc ggcaccacct gggaagcccg cgtcaagctg aactgcctga ccgacggtgc gtggcccgcg ttctggcttc tcaacgacaa ccccgtgcgt ggcggcgagg tcgacctcgt cgagtggtac ggcaaccgag actggccgtc gggcaccacg gtgcacgccc gcctcgacgg cacgtcgttc gcgaccaatc cgcatcccgt cgacagcgac tggcacacct ggcgcatgtc gtggaaaccc gaaggcatgt acttctggaa ggactacgcg ccgggtatgg agccgttctt ctcggttccc gcgaactcgt tgcaggactg gcctttcaac gatcccggtt acaccatggt cccggtgttc aacatcgccg tcggcggctc cggcggacgt gaacccgccg gaggcaccta tccggccgag atgctggtcg actggatccg ggtgttcgcc ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MysmA.18623.a.B2.PS02010
PURIFICATION DATe: 3/6/2014
CONCENTRATION: 15.7mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DPLIGDNPPG PGPAAAAEPT FLFQDEFNGP AGSPPDPAWW YLVPARETIK NPVEWDKPYN MGRYVTDQEH AFQDGKGNLV IRATRGPGTT IQEKYASAKV VGLWRGGIGT TWEARVKLNC LTDGAWPAFW LLNDNPVRGG EVDLVEWYGN RDWPSGTTVH ARLDGTSFAT NPHPVDSDWH TWRMSWKPEG MYFWKDYAPG MEPFFSVPAN SLQDWPFNDP GYTMVPVFNI AVGGSGGREP AGGTYPAEML VDWIRVFAG
Validated NT Sequence
atcttactca cttagccggc gaacacccgg atccagtcga ccagcatctc ggccggatag gtgcctccgg cgggttcacg tccgccggag ccgccgacgg cgatgttgaa caccgggacc atggtgtaac cgggatcgtt gaaaggccag tcctgcaacg agttcgcggg aaccgagaag aacggctcca tacccggcgc gtagtccttc cagaagtaca tgccttcggg tttccacgac atgcgccagg tgtgccagtc gctgtcgacg ggatgcggat tggtcgcgaa cgacgtgccg tcgaggcggg cgtgcaccgt ggtgcccgac ggccagtctc ggttgccgta ccactcgacg aggtcgacct cgccgccacg cacggggttg tcgttgagaa gccagaacgc gggccacgca ccgtcggtca ggcagttcag cttgacgcgg gcttcccagg tggtgccgat gccaccgcgc cacaggccga ccaccttggc actggcgtac ttctcctgga tggtggtacc cggcccccgc gtcgcgcgga tgaccagatt gcccttgccg tcctggaagg catgttcctg gtcggtgaca taccgcccca tgttgtacgg cttgtcccat tcgacggggt tcttgatggt ctcgcgggcg ggcaccaggt accaccacgc gggatccggc ggcgatcccg cgggaccgtt gaactcgtcc tggaacagga aggtgggctc ggctgccgcg gcgggcccgg gaccgggcgg gttatctccg atgagtgggt cggccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHAD PLIGDNPPGP GPAAAAEPTF LFQDEFNGPA GSPPDPAWWY LVPARETIKN PVEWDKPYNM GRYVTDQEHA FQDGKGNLVI RATRGPGTTI QEKYASAKVV GLWRGGIGTT WEARVKLNCL TDGAWPAFWL LNDNPVRGGE VDLVEWYGNR DWPSGTTVHA RLDGTSFATN PHPVDSDWHT WRMSWKPEGM YFWKDYAPGM EPFFSVPANS LQDWPFNDPG YTMVPVFNIA VGGSGGREPA GGTYPAEMLV DWIRVFAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGACC CACTCATCGG AGATAACCCG CCCGGTCCCG GGCCCGCCGC GGCAGCCGAG CCCACCTTCC TGTTCCAGGA CGAGTTCAAC GGTCCCGCGG GATCGCCGCC GGATCCCGCG TGGTGGTACC TGGTGCCCGC CCGCGAGACC ATCAAGAACC CCGTCGAATG GGACAAGCCG TACAACATGG GGCGGTATGT CACCGACCAG GAACATGCCT TCCAGGACGG CAAGGGCAAT CTGGTCATCC GCGCGACGCG GGGGCCGGGT ACCACCATCC AGGAGAAGTA CGCCAGTGCC AAGGTGGTCG GCCTGTGGCG CGGTGGCATC GGCACCACCT GGGAAGCCCG CGTCAAGCTG AACTGCCTGA CCGACGGTGC GTGGCCCGCG TTCTGGCTTC TCAACGACAA CCCCGTGCGT GGCGGCGAGG TCGACCTCGT CGAGTGGTAC GGCAACCGAG ACTGGCCGTC GGGCACCACG GTGCACGCCC GCCTCGACGG CACGTCGTTC GCGACCAATC CGCATCCCGT CGACAGCGAC TGGCACACCT GGCGCATGTC GTGGAAACCC GAAGGCATGT ACTTCTGGAA GGACTACGCG CCGGGTATGG AGCCGTTCTT CTCGGTTCCC GCGAACTCGT TGCAGGACTG GCCTTTCAAC GATCCCGGTT ACACCATGGT CCCGGTGTTC AACATCGCCG TCGGCGGCTC CGGCGGACGT GAACCCGCCG GAGGCACCTA TCCGGCCGAG ATGCTGGTCG ACTGGATCCG GGTGTTCGCC GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for MysmA.18623.a.B2.PS02011
PURIFICATION DATe: 3/6/2014
CONCENTRATION: 30.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DPLIGDNPPG PGPAAAAEPT FLFQDEFNGP AGSPPDPAWW YLVPARETIK NPVEWDKPYN MGRYVTDQEH AFQDGKGNLV IRATRGPGTT IQEKYASAKV VGLWRGGIGT TWEARVKLNC LTDGAWPAFW LLNDNPVRGG EVDLVEWYGN RDWPSGTTVH ARLDGTSFAT NPHPVDSDWH TWRMSWKPEG MYFWKDYAPG MEPFFSVPAN SLQDWPFNDP GYTMVPVFNI AVGGSGGREP AGGTYPAEML VDWIRVFAG
Validated NT Sequence
atcttactca cttagccggc gaacacccgg atccagtcga ccagcatctc ggccggatag gtgcctccgg cgggttcacg tccgccggag ccgccgacgg cgatgttgaa caccgggacc atggtgtaac cgggatcgtt gaaaggccag tcctgcaacg agttcgcggg aaccgagaag aacggctcca tacccggcgc gtagtccttc cagaagtaca tgccttcggg tttccacgac atgcgccagg tgtgccagtc gctgtcgacg ggatgcggat tggtcgcgaa cgacgtgccg tcgaggcggg cgtgcaccgt ggtgcccgac ggccagtctc ggttgccgta ccactcgacg aggtcgacct cgccgccacg cacggggttg tcgttgagaa gccagaacgc gggccacgca ccgtcggtca ggcagttcag cttgacgcgg gcttcccagg tggtgccgat gccaccgcgc cacaggccga ccaccttggc actggcgtac ttctcctgga tggtggtacc cggcccccgc gtcgcgcgga tgaccagatt gcccttgccg tcctggaagg catgttcctg gtcggtgaca taccgcccca tgttgtacgg cttgtcccat tcgacggggt tcttgatggt ctcgcgggcg ggcaccaggt accaccacgc gggatccggc ggcgatcccg cgggaccgtt gaactcgtcc tggaacagga aggtgggctc ggctgccgcg gcgggcccgg gaccgggcgg gttatctccg atgagtgggt cggccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHAD PLIGDNPPGP GPAAAAEPTF LFQDEFNGPA GSPPDPAWWY LVPARETIKN PVEWDKPYNM GRYVTDQEHA FQDGKGNLVI RATRGPGTTI QEKYASAKVV GLWRGGIGTT WEARVKLNCL TDGAWPAFWL LNDNPVRGGE VDLVEWYGNR DWPSGTTVHA RLDGTSFATN PHPVDSDWHT WRMSWKPEGM YFWKDYAPGM EPFFSVPANS LQDWPFNDPG YTMVPVFNIA VGGSGGREPA GGTYPAEMLV DWIRVFAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGACC CACTCATCGG AGATAACCCG CCCGGTCCCG GGCCCGCCGC GGCAGCCGAG CCCACCTTCC TGTTCCAGGA CGAGTTCAAC GGTCCCGCGG GATCGCCGCC GGATCCCGCG TGGTGGTACC TGGTGCCCGC CCGCGAGACC ATCAAGAACC CCGTCGAATG GGACAAGCCG TACAACATGG GGCGGTATGT CACCGACCAG GAACATGCCT TCCAGGACGG CAAGGGCAAT CTGGTCATCC GCGCGACGCG GGGGCCGGGT ACCACCATCC AGGAGAAGTA CGCCAGTGCC AAGGTGGTCG GCCTGTGGCG CGGTGGCATC GGCACCACCT GGGAAGCCCG CGTCAAGCTG AACTGCCTGA CCGACGGTGC GTGGCCCGCG TTCTGGCTTC TCAACGACAA CCCCGTGCGT GGCGGCGAGG TCGACCTCGT CGAGTGGTAC GGCAACCGAG ACTGGCCGTC GGGCACCACG GTGCACGCCC GCCTCGACGG CACGTCGTTC GCGACCAATC CGCATCCCGT CGACAGCGAC TGGCACACCT GGCGCATGTC GTGGAAACCC GAAGGCATGT ACTTCTGGAA GGACTACGCG CCGGGTATGG AGCCGTTCTT CTCGGTTCCC GCGAACTCGT TGCAGGACTG GCCTTTCAAC GATCCCGGTT ACACCATGGT CCCGGTGTTC AACATCGCCG TCGGCGGCTC CGGCGGACGT GAACCCGCCG GAGGCACCTA TCCGGCCGAG ATGCTGGTCG ACTGGATCCG GGTGTTCGCC GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT