MyfoA.18623.a
Glycosyl hydrolase family protein (Rv0315 ortholog)
| CENTER ID: | MyfoA.18623.a |
| ORGANISM: | Mycobacterium fortuitum ATCC 6841 / DSM 46621 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyfoA.18623.a.B2.GE38108 | mature protein | 29 | 279 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyfoA.18623.a.B2.PS02082 | mature protein | 29 | 279 |
Structures
4W65
DEPOSITED: 8/20/2014
DETERMINATION: XRay
CLONE: MyfoA.18623.a.B2.GE38108
PROTEIN: MyfoA.18623.a.B2.PS02082
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| RefSeq: | WP_003885076.1 |
| UniProt: | K0UYS3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANLDRRKMM MLSGLGVMAA ALPAPHAQAL PTKPQTPPVP VPSAPQAAVN YVFADEFDGP AGSSPNTSKW TIAKARETIK DPTYWEQPGR IGQYRDDRKN AFLDGKGNLV IRATKEGDTY YGAKLASVWE GGAGHTWEAR IKFNCLTAGA WPAFWLGTLG EGELDIVEWY GNGKWPSATT VHAKSNGGEW ETHNIAVDTA WHTWRTQWDA NGARFWQDYT EGAKPYFEVS ASQLPDWPFN QPGYTMFVVL NLAVAGSGGE DPSGGTYPAD MLVDYVRVW
NT Sequence
atggccaact tggatcgtcg aaaaatgatg atgctgtcag gtctgggcgt catggcggcg gcactgcccg ccccgcacgc ccaggcactc ccgaccaagc cccagacacc gcccgtcccg gtgccctcag cacctcaagc ggcagtgaac tacgtgttcg cggacgaatt cgacgggccg gcgggctcgt cgccgaacac ctcgaagtgg accatcgcca aggctcgcga gaccatcaaa gaccccacct actgggagca gcccggtcgc atcgggcagt accgggatga ccgcaagaac gccttcctcg acggcaaggg gaatctcgtc atccgggcca ccaaggaagg cgacacctat tacggcgcca agctggccag cgtgtgggaa ggcggtgccg ggcacacctg ggaagcgcgg atcaagttca actgcctgac cgcgggcgcc tggccggcct tctggctggg caccctgggc gagggcgagc tggacatcgt cgagtggtac ggcaacggca aatggccgtc ggccaccacc gtgcacgcca agtcgaacgg cggcgaatgg gagacccaca acatcgctgt cgacaccgca tggcacacct ggcgtaccca gtgggacgcc aacggcgccc ggttctggca ggactacacc gaaggcgcca agccgtactt cgaggtttcg gccagccagc tgcccgactg gccgttcaac cagccgggct acacgatgtt cgtggtgttg aacctcgccg tcgcgggctc gggcggagaa gaccccagcg ggggcaccta cccggccgac atgctcgtgg actacgtgcg cgtctggtag
Details for MyfoA.18623.a.B2.GE38108
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA LPTKPQTPPV PVPSAPQAAV NYVFADEFDG PAGSSPNTSK WTIAKARETI KDPTYWEQPG RIGQYRDDRK NAFLDGKGNL VIRATKEGDT YYGAKLASVW EGGAGHTWEA RIKFNCLTAG AWPAFWLGTL GEGELDIVEW YGNGKWPSAT TVHAKSNGGE WETHNIAVDT AWHTWRTQWD ANGARFWQDY TEGAKPYFEV SASQLPDWPF NQPGYTMFVV LNLAVAGSGG EDPSGGTYPA DMLVDYVRVW
Validated NT Sequence
atcttactca cttaccagac gcgcacgtag tccacgagca tgtcggccgg gtaggtgccc ccgctggggt cttctccgcc cgagcccgcg acggcgaggt tcaacaccac gaacatcgtg tagcccggct ggttgaacgg ccagtcgggc agctggctgg ccgaaacctc gaagtacggc ttggcgcctt cggtgtagtc ctgccagaac cgggcgccgt tggcgtccca ctgggtacgc caggtgtgcc atgcggtgtc gacagcgatg ttgtgggtct cccattcgcc gccgttcgac ttggcgtgca cggtggtggc cgacggccat ttgccgttgc cgtaccactc gactatgtcc agctcgccct cgcccagggt gcccagccag aaggccggcc aggcgcccgc ggtcaggcag ttgaacttga tccgcgcttc ccaggtgtgc ccggcaccgc cttcccacac gctggccagc ttggcgccgt aataggtgtc gccttccttg gtggcccgga tgacgagatt ccccttgccg tcgaggaagg cgttcttgcg gtcatcccgg tactgcccga tgcgaccggg ctgctcccag taggtggggt ctttgatggt ctcgcgagcc ttggcgatgg tccacttcga ggtgttcggc gacgagcccg ccggcccgtc gaattcgtcc gcgaacacgt agttcactgc cgcttgaggt gctgagggca ccgggacggg cggtgtctgg ggcttggtcg ggagtgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAL PTKPQTPPVP VPSAPQAAVN YVFADEFDGP AGSSPNTSKW TIAKARETIK DPTYWEQPGR IGQYRDDRKN AFLDGKGNLV IRATKEGDTY YGAKLASVWE GGAGHTWEAR IKFNCLTAGA WPAFWLGTLG EGELDIVEWY GNGKWPSATT VHAKSNGGEW ETHNIAVDTA WHTWRTQWDA NGARFWQDYT EGAKPYFEVS ASQLPDWPFN QPGYTMFVVL NLAVAGSGGE DPSGGTYPAD MLVDYVRVW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcactcc cgaccaagcc ccagacaccg cccgtcccgg tgccctcagc acctcaagcg gcagtgaact acgtgttcgc ggacgaattc gacgggccgg cgggctcgtc gccgaacacc tcgaagtgga ccatcgccaa ggctcgcgag accatcaaag accccaccta ctgggagcag cccggtcgca tcgggcagta ccgggatgac cgcaagaacg ccttcctcga cggcaagggg aatctcgtca tccgggccac caaggaaggc gacacctatt acggcgccaa gctggccagc gtgtgggaag gcggtgccgg gcacacctgg gaagcgcgga tcaagttcaa ctgcctgacc gcgggcgcct ggccggcctt ctggctgggc accctgggcg agggcgagct ggacatcgtc gagtggtacg gcaacggcaa atggccgtcg gccaccaccg tgcacgccaa gtcgaacggc ggcgaatggg agacccacaa catcgctgtc gacaccgcat ggcacacctg gcgtacccag tgggacgcca acggcgcccg gttctggcag gactacaccg aaggcgccaa gccgtacttc gaggtttcgg ccagccagct gcccgactgg ccgttcaacc agccgggcta cacgatgttc gtggtgttga acctcgccgt cgcgggctcg ggcggagaag accccagcgg gggcacctac ccggccgaca tgctcgtgga ctacgtgcgc gtctggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MyfoA.18623.a.B2.PS02082
| PURIFICATION DATe: | 6/19/2014 |
| CONCENTRATION: | 24.4mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA LPTKPQTPPV PVPSAPQAAV NYVFADEFDG PAGSSPNTSK WTIAKARETI KDPTYWEQPG RIGQYRDDRK NAFLDGKGNL VIRATKEGDT YYGAKLASVW EGGAGHTWEA RIKFNCLTAG AWPAFWLGTL GEGELDIVEW YGNGKWPSAT TVHAKSNGGE WETHNIAVDT AWHTWRTQWD ANGARFWQDY TEGAKPYFEV SASQLPDWPF NQPGYTMFVV LNLAVAGSGG EDPSGGTYPA DMLVDYVRVW
Validated NT Sequence
atcttactca cttaccagac gcgcacgtag tccacgagca tgtcggccgg gtaggtgccc ccgctggggt cttctccgcc cgagcccgcg acggcgaggt tcaacaccac gaacatcgtg tagcccggct ggttgaacgg ccagtcgggc agctggctgg ccgaaacctc gaagtacggc ttggcgcctt cggtgtagtc ctgccagaac cgggcgccgt tggcgtccca ctgggtacgc caggtgtgcc atgcggtgtc gacagcgatg ttgtgggtct cccattcgcc gccgttcgac ttggcgtgca cggtggtggc cgacggccat ttgccgttgc cgtaccactc gactatgtcc agctcgccct cgcccagggt gcccagccag aaggccggcc aggcgcccgc ggtcaggcag ttgaacttga tccgcgcttc ccaggtgtgc ccggcaccgc cttcccacac gctggccagc ttggcgccgt aataggtgtc gccttccttg gtggcccgga tgacgagatt ccccttgccg tcgaggaagg cgttcttgcg gtcatcccgg tactgcccga tgcgaccggg ctgctcccag taggtggggt ctttgatggt ctcgcgagcc ttggcgatgg tccacttcga ggtgttcggc gacgagcccg ccggcccgtc gaattcgtcc gcgaacacgt agttcactgc cgcttgaggt gctgagggca ccgggacggg cggtgtctgg ggcttggtcg ggagtgccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHAL PTKPQTPPVP VPSAPQAAVN YVFADEFDGP AGSSPNTSKW TIAKARETIK DPTYWEQPGR IGQYRDDRKN AFLDGKGNLV IRATKEGDTY YGAKLASVWE GGAGHTWEAR IKFNCLTAGA WPAFWLGTLG EGELDIVEWY GNGKWPSATT VHAKSNGGEW ETHNIAVDTA WHTWRTQWDA NGARFWQDYT EGAKPYFEVS ASQLPDWPFN QPGYTMFVVL NLAVAGSGGE DPSGGTYPAD MLVDYVRVW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCACTCC CGACCAAGCC CCAGACACCG CCCGTCCCGG TGCCCTCAGC ACCTCAAGCG GCAGTGAACT ACGTGTTCGC GGACGAATTC GACGGGCCGG CGGGCTCGTC GCCGAACACC TCGAAGTGGA CCATCGCCAA GGCTCGCGAG ACCATCAAAG ACCCCACCTA CTGGGAGCAG CCCGGTCGCA TCGGGCAGTA CCGGGATGAC CGCAAGAACG CCTTCCTCGA CGGCAAGGGG AATCTCGTCA TCCGGGCCAC CAAGGAAGGC GACACCTATT ACGGCGCCAA GCTGGCCAGC GTGTGGGAAG GCGGTGCCGG GCACACCTGG GAAGCGCGGA TCAAGTTCAA CTGCCTGACC GCGGGCGCCT GGCCGGCCTT CTGGCTGGGC ACCCTGGGCG AGGGCGAGCT GGACATCGTC GAGTGGTACG GCAACGGCAA ATGGCCGTCG GCCACCACCG TGCACGCCAA GTCGAACGGC GGCGAATGGG AGACCCACAA CATCGCTGTC GACACCGCAT GGCACACCTG GCGTACCCAG TGGGACGCCA ACGGCGCCCG GTTCTGGCAG GACTACACCG AAGGCGCCAA GCCGTACTTC GAGGTTTCGG CCAGCCAGCT GCCCGACTGG CCGTTCAACC AGCCGGGCTA CACGATGTTC GTGGTGTTGA ACCTCGCCGT CGCGGGCTCG GGCGGAGAAG ACCCCAGCGG GGGCACCTAC CCGGCCGACA TGCTCGTGGA CTACGTGCGC GTCTGGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T