MyabA.18623.a
Putative beta-glucanase (Rv0315 ortholog)
| CENTER ID: | MyabA.18623.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.18623.a.B2.GE37327 | mature protein | 17 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.18623.a.B2.PS02117 | mature protein | 17 | 265 |
Structures
5DXD
DEPOSITED: 9/23/2015
DETERMINATION: XRay
CLONE: MyabA.18623.a.B2.GE37327
PROTEIN: MyabA.18623.a.B2.PS02117
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|36809.5.peg.1873 |
| RefSeq: | YP_001702577.1 |
| UniProt: | B1MNM2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFGMAAAAL PLPKAQADRV HPPAAPPDAQ SSGFVFRDEF DGPAGSAPDG SKWVAAKFRE RIKNPVFWDR PENMGEYRDS RTNIFLDGNS NLVIRATKEG NKYFSGKLHG TFRGGMGHTF EARIKFNCLT DGCWPAWWLL NDNPERGGEI DMAEWYGNRD WPSGTTVHAR LDGTSFETLP VPIDSNWHTW RCTWTEAGLY FWMDYHDGME PYLTVDANSL DDWPFNDPGY TLQPMLNLAV AGSGGGDPAG GSYPAEMLVD YVRVW
NT Sequence
atgggcttcg gcatggccgc agctgctttg ccccttccca aggctcaggc cgatcgggtg catcctccgg cggcaccgcc cgacgcccag tccagcggtt ttgtgttccg tgacgagttc gacggccccg ccggttcggc gcccgacggt tccaagtggg tggccgcgaa gttccgcgag cgcatcaaga acccggtgtt ctgggatcgc cccgagaaca tgggcgagta ccgcgacagc cgcaccaaca tcttcctcga cggaaactcc aacctggtga tccgtgcgac caaggaaggc aacaagtact tcagcggcaa gctgcacggc accttccgcg gcggcatggg ccacaccttc gaggcacgca tcaagttcaa ctgcttgacc gacggttgct ggccggcctg gtggctgctg aacgacaacc cggagcgcgg cggcgagatc gacatggccg agtggtacgg caaccgcgac tggccctcgg gcaccacggt gcacgcacgc ctggacggca cctccttcga gacgctgccg gtgcccatcg acagcaactg gcacacctgg cgctgcacct ggaccgaggc cggactgtac ttctggatgg actaccacga cggcatggag ccgtacctga cggtcgacgc caattccctt gacgattggc cgtttaacga tccgggctac acgctgcagc cgatgctgaa cctggctgtc gccggttccg gcggtggcga tccggccggc ggctcgtacc cggcggagat gctcgtcgac tacgtgcgcg tctggtag
Details for MyabA.18623.a.B2.GE37327
| HARVESTED ON: | 8/28/2013 |
| SEQUENCED ON: | 9/3/2013 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA DRVHPPAAPP DAQSSGFVFR DEFDGPAGSA PDGSKWVAAK FRERIKNPVF WDRPENMGEY RDSRTNIFLD GNSNLVIRAT KEGNKYFSGK LHGTFRGGMG HTFEARIKFN CLTDGCWPAW WLLNDNPERG GEIDMAEWYG NRDWPSGTTV HARLDGTSFE TLPVPIDSNW HTWRCTWTEA GLYFWMDYHD GMEPYLTVDA NSLDDWPFND PGYTLQPMLN LAVAGSGGGD PAGGSYPAEM LVDYVRVW
Validated NT Sequence
atcttactca cttaccagac gcgcacgtag tcgacgagca tctccgccgg gtacgagccg ccggccggat cgccaccgcc ggaaccggcg acagccaggt tcagcatcgg ctgcagcgtg tagcccggat cgttaaacgg ccaatcgtca agggaattgg cgtcgaccgt caggtacggc tccatgccgt cgtggtagtc catccagaag tacagtccgg cctcggtcca ggtgcagcgc caggtgtgcc agttgctgtc gatgggcacc ggcagcgtct cgaaggaggt gccgtccagg cgtgcgtgca ccgtggtgcc cgagggccag tcgcggttgc cgtaccactc ggccatgtcg atctcgccgc cgcgctccgg gttgtcgttc agcagccacc aggccggcca gcaaccgtcg gtcaagcagt tgaacttgat gcgtgcctcg aaggtgtggc ccatgccgcc gcggaaggtg ccgtgcagct tgccgctgaa gtacttgttg ccttccttgg tcgcacggat caccaggttg gagtttccgt cgaggaagat gttggtgcgg ctgtcgcggt actcgcccat gttctcgggg cgatcccaga acaccgggtt cttgatgcgc tcgcggaact tcgcggccac ccacttggaa ccgtcgggcg ccgaaccggc ggggccgtcg aactcgtcac ggaacacaaa accgctggac tgggcgtcgg gcggtgccgc cggaggatgc acccgatcgg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAD RVHPPAAPPD AQSSGFVFRD EFDGPAGSAP DGSKWVAAKF RERIKNPVFW DRPENMGEYR DSRTNIFLDG NSNLVIRATK EGNKYFSGKL HGTFRGGMGH TFEARIKFNC LTDGCWPAWW LLNDNPERGG EIDMAEWYGN RDWPSGTTVH ARLDGTSFET LPVPIDSNWH TWRCTWTEAG LYFWMDYHDG MEPYLTVDAN SLDDWPFNDP GYTLQPMLNL AVAGSGGGDP AGGSYPAEML VDYVRVW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatc gggtgcatcc tccggcggca ccgcccgacg cccagtccag cggttttgtg ttccgtgacg agttcgacgg ccccgccggt tcggcgcccg acggttccaa gtgggtggcc gcgaagttcc gcgagcgcat caagaacccg gtgttctggg atcgccccga gaacatgggc gagtaccgcg acagccgcac caacatcttc ctcgacggaa actccaacct ggtgatccgt gcgaccaagg aaggcaacaa gtacttcagc ggcaagctgc acggcacctt ccgcggcggc atgggccaca ccttcgaggc acgcatcaag ttcaactgct tgaccgacgg ttgctggccg gcctggtggc tgctgaacga caacccggag cgcggcggcg agatcgacat ggccgagtgg tacggcaacc gcgactggcc ctcgggcacc acggtgcacg cacgcctgga cggcacctcc ttcgagacgc tgccggtgcc catcgacagc aactggcaca cctggcgctg cacctggacc gaggccggac tgtacttctg gatggactac cacgacggca tggagccgta cctgacggtc gacgccaatt cccttgacga ttggccgttt aacgatccgg gctacacgct gcagccgatg ctgaacctgg ctgtcgccgg ttccggcggt ggcgatccgg ccggcggctc gtacccggcg gagatgctcg tcgactacgt gcgcgtctgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyabA.18623.a.B2.PS02117
| PURIFICATION DATe: | 7/24/2014 |
| CONCENTRATION: | 34.23mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DRVHPPAAPP DAQSSGFVFR DEFDGPAGSA PDGSKWVAAK FRERIKNPVF WDRPENMGEY RDSRTNIFLD GNSNLVIRAT KEGNKYFSGK LHGTFRGGMG HTFEARIKFN CLTDGCWPAW WLLNDNPERG GEIDMAEWYG NRDWPSGTTV HARLDGTSFE TLPVPIDSNW HTWRCTWTEA GLYFWMDYHD GMEPYLTVDA NSLDDWPFND PGYTLQPMLN LAVAGSGGGD PAGGSYPAEM LVDYVRVW
Validated NT Sequence
atcttactca cttaccagac gcgcacgtag tcgacgagca tctccgccgg gtacgagccg ccggccggat cgccaccgcc ggaaccggcg acagccaggt tcagcatcgg ctgcagcgtg tagcccggat cgttaaacgg ccaatcgtca agggaattgg cgtcgaccgt caggtacggc tccatgccgt cgtggtagtc catccagaag tacagtccgg cctcggtcca ggtgcagcgc caggtgtgcc agttgctgtc gatgggcacc ggcagcgtct cgaaggaggt gccgtccagg cgtgcgtgca ccgtggtgcc cgagggccag tcgcggttgc cgtaccactc ggccatgtcg atctcgccgc cgcgctccgg gttgtcgttc agcagccacc aggccggcca gcaaccgtcg gtcaagcagt tgaacttgat gcgtgcctcg aaggtgtggc ccatgccgcc gcggaaggtg ccgtgcagct tgccgctgaa gtacttgttg ccttccttgg tcgcacggat caccaggttg gagtttccgt cgaggaagat gttggtgcgg ctgtcgcggt actcgcccat gttctcgggg cgatcccaga acaccgggtt cttgatgcgc tcgcggaact tcgcggccac ccacttggaa ccgtcgggcg ccgaaccggc ggggccgtcg aactcgtcac ggaacacaaa accgctggac tgggcgtcgg gcggtgccgc cggaggatgc acccgatcgg ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHAD RVHPPAAPPD AQSSGFVFRD EFDGPAGSAP DGSKWVAAKF RERIKNPVFW DRPENMGEYR DSRTNIFLDG NSNLVIRATK EGNKYFSGKL HGTFRGGMGH TFEARIKFNC LTDGCWPAWW LLNDNPERGG EIDMAEWYGN RDWPSGTTVH ARLDGTSFET LPVPIDSNWH TWRCTWTEAG LYFWMDYHDG MEPYLTVDAN SLDDWPFNDP GYTLQPMLNL AVAGSGGGDP AGGSYPAEML VDYVRVW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGATC GGGTGCATCC TCCGGCGGCA CCGCCCGACG CCCAGTCCAG CGGTTTTGTG TTCCGTGACG AGTTCGACGG CCCCGCCGGT TCGGCGCCCG ACGGTTCCAA GTGGGTGGCC GCGAAGTTCC GCGAGCGCAT CAAGAACCCG GTGTTCTGGG ATCGCCCCGA GAACATGGGC GAGTACCGCG ACAGCCGCAC CAACATCTTC CTCGACGGAA ACTCCAACCT GGTGATCCGT GCGACCAAGG AAGGCAACAA GTACTTCAGC GGCAAGCTGC ACGGCACCTT CCGCGGCGGC ATGGGCCACA CCTTCGAGGC ACGCATCAAG TTCAACTGCT TGACCGACGG TTGCTGGCCG GCCTGGTGGC TGCTGAACGA CAACCCGGAG CGCGGCGGCG AGATCGACAT GGCCGAGTGG TACGGCAACC GCGACTGGCC CTCGGGCACC ACGGTGCACG CACGCCTGGA CGGCACCTCC TTCGAGACGC TGCCGGTGCC CATCGACAGC AACTGGCACA CCTGGCGCTG CACCTGGACC GAGGCCGGAC TGTACTTCTG GATGGACTAC CACGACGGCA TGGAGCCGTA CCTGACGGTC GACGCCAATT CCCTTGACGA TTGGCCGTTT AACGATCCGG GCTACACGCT GCAGCCGATG CTGAACCTGG CTGTCGCCGG TTCCGGCGGT GGCGATCCGG CCGGCGGCTC GTACCCGGCG GAGATGCTCG TCGACTACGT GCGCGTCTGG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT