SSGCID: Target Page

MyfoA.18623.b

Beta-1,3-glucanase (Rv0315 ortholog)

CENTER ID:	MyfoA.18623.b
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.18623.b.B2.GE38109	mature protein		26	289

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.550
RefSeq:	WP_003880422.1
UniProt:	K0V9N4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRRSVMFMM GLGVAAAALP AGTANADPAA PGPAAPGAPT PPAAAAAQPT FLFQDEFNGP AGSPPDPTWW HLIPERETIK NPVEWDKPFN MGRYVTDTEH AFQDGKGNLV IRATRGPGTT IQEKYASAKV VGNWRGGIGT TWEARVKLNC LTDGAWPAFW LLNDDPVRGG EVDLVEWYGN RDWPSGTTVH ARLDGESFAT DPHPIDSGWH TWRMSWTEHG MYFWKDYAPG MEPFFEVPAN SLEDWPFNDP GYTMVPVFNI AVGGSGGRDP SGGSYPAEML VDWIRVFQG

NT Sequence

atggatcgtc gcagtgtgat gttcatgatg ggtctggggg tggcagccgc agcgctgcct gccgggacgg ccaacgccga cccggccgca cctggtcccg ccgcgcccgg agctcccacc ccgccggctg ccgcagcggc gcagccgacc ttcctgttcc aggatgagtt caacggtccg gccggctctc cgccggaccc gacgtggtgg cacctgatcc cggagcgtga gaccatcaag aacccggtcg agtgggacaa gccgttcaac atgggccggt atgtcaccga caccgagcac gcattccagg acggcaaggg caacctggtg attcgcgcga cgcgcggccc ggggaccacg atccaggaga agtacgccag cgccaaggtc gtcggcaact ggcgcggtgg catcggcacc acctgggagg cccgggtcaa gctcaactgc ctgaccgacg gtgcctggcc cgccttctgg ctgctgaatg acgatcccgt tcgcggtggc gaggtcgacc tggtggagtg gtacggcaac cgtgactggc cgtcgggcac caccgtgcac gcgcgcctgg acggcgagtc gttcgccacc gacccgcacc ccatcgacag cggctggcac acctggcgga tgtcgtggac ggaacacggc atgtacttct ggaaggacta tgccccgggc atggaaccct tcttcgaggt gcccgcaaac tcgttggaag actggccctt caacgatccg ggctacacca tggtgccggt gttcaacatc gctgtcggcg gctcgggcgg ccgtgacccg agcggtggca gctacccggc cgagatgctg gtcgactgga tccgcgtctt ccagggctga

Details for MyfoA.18623.b.B2.GE38109

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	30kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	84
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DPAAPGPAAP GAPTPPAAAA AQPTFLFQDE FNGPAGSPPD PTWWHLIPER ETIKNPVEWD KPFNMGRYVT DTEHAFQDGK GNLVIRATRG PGTTIQEKYA SAKVVGNWRG GIGTTWEARV KLNCLTDGAW PAFWLLNDDP VRGGEVDLVE WYGNRDWPSG TTVHARLDGE SFATDPHPID SGWHTWRMSW TEHGMYFWKD YAPGMEPFFE VAQTRWKTGP STIRATPWCR CSTSLSAARA AVTRAVAATR PRCWSTGSAS SRAK

Validated NT Sequence

annatnnntt nntttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggccgac ccggccgcac ctggtcccgc cgcgcccgga gctcccaccc cgccggctgc cgcagcggcg cagccgacct tcctgttcca ggatgagttc aacggtccgg ccggctctcc gccggacccg acgtggtggc acctgatccc ggagcgtgag accatcaaga acccggtcga gtgggacaag ccgttcaaca tgggccggta tgtcaccgac accgagcacg cattccagga cggcaagggc aacctggtga ttcgcgcgac gcgcggcccg gggaccacga tccaggagaa gtacgccagc gccaaggtcg tcggcaactg gcgcggtggc atcggcacca cctgggaggc ccgggtcaag ctcaactgcc tgaccgacgg tgcctggccc gccttctggc tgctgaatga cgatcccgtt cgcggtggcg aggtcgacct ggtggagtgg tacggcaacc gtgactggcc gtcgggcacc accgtgcacg cgcgcctgga cggcgagtcg ttcgccaccg acccgcaccc catcgacagc ggctggcaca cctggcggat gtcgtggacg gaacacggca tgtacttctg gaaggactat gccccgggca tggaaccctt cttcgaggtg gcgcaaactc gttggaagac tggcccttca acgatccggg ctacaccatg gtgccggtgt tcaacatcgc tgtcggcggc tcgggcggcc gtgacccgag cggtggcagc tacccggccg agatgctggt cgactggatc cgcgtcttcc agggctaagt gagtaagata ggatcnnctg nnnannnncc cccaaganan nnnnnannnn natccggtcg ancaagcatc tcggccgggt agctgccnac cgcttcgggg tcanggccgc ccncagcnnc cnngnnatnn tgaatnnnnn gacnccntgg tgtaggccgg aatcngtgat agggcaangc ntccannatn tctannnnnc ctntgcanna aggttnnnnn ngntggcntn nccttnnaaa nnnctgnccn gtatnnacnn nnnnnnnctn cngnccngcn nttncnnnnn nnngccnnnn ntgnnnnnnn nnncgnncgn tnnnnnaccg ncaagncngc ngnnncnnnn nnnncnnnnn nnnnnngnnn nnnncncnnn nnnnnctnnc nannnnnnnn nnngnnnnnn nng

Expected Protein Sequence

MAHHHHHHAD PAAPGPAAPG APTPPAAAAA QPTFLFQDEF NGPAGSPPDP TWWHLIPERE TIKNPVEWDK PFNMGRYVTD TEHAFQDGKG NLVIRATRGP GTTIQEKYAS AKVVGNWRGG IGTTWEARVK LNCLTDGAWP AFWLLNDDPV RGGEVDLVEW YGNRDWPSGT TVHARLDGES FATDPHPIDS GWHTWRMSWT EHGMYFWKDY APGMEPFFEV PANSLEDWPF NDPGYTMVPV FNIAVGGSGG RDPSGGSYPA EMLVDWIRVF QG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgacc cggccgcacc tggtcccgcc gcgcccggag ctcccacccc gccggctgcc gcagcggcgc agccgacctt cctgttccag gatgagttca acggtccggc cggctctccg ccggacccga cgtggtggca cctgatcccg gagcgtgaga ccatcaagaa cccggtcgag tgggacaagc cgttcaacat gggccggtat gtcaccgaca ccgagcacgc attccaggac ggcaagggca acctggtgat tcgcgcgacg cgcggcccgg ggaccacgat ccaggagaag tacgccagcg ccaaggtcgt cggcaactgg cgcggtggca tcggcaccac ctgggaggcc cgggtcaagc tcaactgcct gaccgacggt gcctggcccg ccttctggct gctgaatgac gatcccgttc gcggtggcga ggtcgacctg gtggagtggt acggcaaccg tgactggccg tcgggcacca ccgtgcacgc gcgcctggac ggcgagtcgt tcgccaccga cccgcacccc atcgacagcg gctggcacac ctggcggatg tcgtggacgg aacacggcat gtacttctgg aaggactatg ccccgggcat ggaacccttc ttcgaggtgc ccgcaaactc gttggaagac tggcccttca acgatccggg ctacaccatg gtgccggtgt tcaacatcgc tgtcggcggc tcgggcggcc gtgacccgag cggtggcagc tacccggccg agatgctggt cgactggatc cgcgtcttcc agggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat